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The map label for this gene is aadR [H]

Identifier: 146300290

GI number: 146300290

Start: 3017979

End: 3018659

Strand: Reverse

Name: aadR [H]

Synonym: Fjoh_2535

Alternate gene names: 146300290

Gene position: 3018659-3017979 (Counterclockwise)

Preceding gene: 146300297

Following gene: 146300286

Centisome position: 49.51

GC content: 34.07

Gene sequence:

>681_bases
ATGAGTAAATGTGATCAATGTATCGTTAGACAGCTTTCTTCATTAAAGGCTCTTAATAAAGATGAAGTTGTAAAACTTGC
CAGCAGTAAAACGACTTACACTATTAAAAAAGGTGAGGCTATTTTTGAAGAAGGTGAAGTTACCAACGGCGTTTTTTGTG
TTAAGGACGGAGTTGGAAAACTATCTAAATTAAGCGCCAACGGAAAAGACCAGATTGTAAAACTTGTAAAATCTGGTGAA
CTTTTAGGCCAGCGTTCTATGATTAGCAATGAACCAGCCAATTTATCTGCAAAAGCAATTGCTGATATGGAAGTTTGTTT
TATTCCGAAATCTGAAATTATTCATTTCTTTAATAACAACAATCAATTTTCTTTGAACCTGATGCAGTCTGTATGCGAAG
ATTTGAAGGAATCTGATAATGACAAAATCGCTTTAGTTCAAAAAACGGTAAAACAGCGTCTGGCTGAAACCTTACTGCAG
CTTCATGATGATTTTGGTGAAAATCCAGATAAAACCCTTAAAGTACAGCTTACCAGAGAAGAACTTGCCGGAATAATTGG
TACTGCAACTGAAAGCTGTATTCGATTACTATCTGATTTTAATAAATTGGAATTGATTGAATTAGTTGGTAAAAAAATTA
TGCTGAAAAACGTAAAAGCCTTAAAAAAACTGGCAGAATAG

Upstream 100 bases:

>100_bases
TCAAACAGCGATAATCACTTTTTTATGTATTTTTTAGACAATAAAATTACCAAGAAAGCCCAATATTGGTTAATTTTGCA
TCAAACTTTTTTTAATTGCT

Downstream 100 bases:

>100_bases
TTTTATTGCTTTTTCGCTTCATTCCAAAAAACATCCATTTCGGCCAGAGTCATATCCATTAAAGGTTTTCCTAATTCTGA
AGCTTTGCTTTCTAAATATT

Product: CRP/FNR family transcriptional regulator

Products: NA

Alternate protein names: Anaerobic aromatic degradation regulator [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSKCDQCIVRQLSSLKALNKDEVVKLASSKTTYTIKKGEAIFEEGEVTNGVFCVKDGVGKLSKLSANGKDQIVKLVKSGE
LLGQRSMISNEPANLSAKAIADMEVCFIPKSEIIHFFNNNNQFSLNLMQSVCEDLKESDNDKIALVQKTVKQRLAETLLQ
LHDDFGENPDKTLKVQLTREELAGIIGTATESCIRLLSDFNKLELIELVGKKIMLKNVKALKKLAE

Sequences:

>Translated_226_residues
MSKCDQCIVRQLSSLKALNKDEVVKLASSKTTYTIKKGEAIFEEGEVTNGVFCVKDGVGKLSKLSANGKDQIVKLVKSGE
LLGQRSMISNEPANLSAKAIADMEVCFIPKSEIIHFFNNNNQFSLNLMQSVCEDLKESDNDKIALVQKTVKQRLAETLLQ
LHDDFGENPDKTLKVQLTREELAGIIGTATESCIRLLSDFNKLELIELVGKKIMLKNVKALKKLAE
>Mature_225_residues
SKCDQCIVRQLSSLKALNKDEVVKLASSKTTYTIKKGEAIFEEGEVTNGVFCVKDGVGKLSKLSANGKDQIVKLVKSGEL
LGQRSMISNEPANLSAKAIADMEVCFIPKSEIIHFFNNNNQFSLNLMQSVCEDLKESDNDKIALVQKTVKQRLAETLLQL
HDDFGENPDKTLKVQLTREELAGIIGTATESCIRLLSDFNKLELIELVGKKIMLKNVKALKKLAE

Specific function: Transcriptional activator of anaerobic gene expression. For aromatic acid degradation. Also required for the anaerobic degradation of benzoate [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=227, Percent_Identity=25.9911894273128, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25018; Mature: 24887

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKCDQCIVRQLSSLKALNKDEVVKLASSKTTYTIKKGEAIFEEGEVTNGVFCVKDGVGK
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCEEEEECCCCH
LSKLSANGKDQIVKLVKSGELLGQRSMISNEPANLSAKAIADMEVCFIPKSEIIHFFNNN
HHHHCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHCCCEEECCHHHHEEEECCC
NQFSLNLMQSVCEDLKESDNDKIALVQKTVKQRLAETLLQLHDDFGENPDKTLKVQLTRE
CEEEHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHH
ELAGIIGTATESCIRLLSDFNKLELIELVGKKIMLKNVKALKKLAE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKCDQCIVRQLSSLKALNKDEVVKLASSKTTYTIKKGEAIFEEGEVTNGVFCVKDGVGK
CHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCEEEEECCCCH
LSKLSANGKDQIVKLVKSGELLGQRSMISNEPANLSAKAIADMEVCFIPKSEIIHFFNNN
HHHHCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHCCCEEECCHHHHEEEECCC
NQFSLNLMQSVCEDLKESDNDKIALVQKTVKQRLAETLLQLHDDFGENPDKTLKVQLTRE
CEEEHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHH
ELAGIIGTATESCIRLLSDFNKLELIELVGKKIMLKNVKALKKLAE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1522059; 10094687 [H]