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The map label for this gene is yubD [H]

Identifier: 145301515

GI number: 145301515

Start: 130148

End: 130792

Strand: Reverse

Name: yubD [H]

Synonym: ASA_P5G137

Alternate gene names: 145301515

Gene position: 130792-130148 (Counterclockwise)

Preceding gene: 145301518

Following gene: 145301514

Centisome position: 84.33

GC content: 57.36

Gene sequence:

>645_bases
ATGTCACGATTCATGTTGGGTGATAGTGTTCAGATCATGTCTACGTTTCCAGGTGCCGCAGTGGACTTCATTTTGACCGA
TCCGCCGTACCTGGTCGGATTTAAAGACCGCTCCGGGCGATCGATTGCCAATGACGTTAATGATGAATGGGTGCTGCCTG
CCTTTACGCAGATGTATCGGGTGTTAAAGCCCCACTCGCTGGCGGTGAGTTTCTACGGCTGGAACCGGGTAGATACCTTT
ATGGCCGCCTGGAAGCAGGCCGGGTTTCGGGTTGTCGGTCATCTGGTGTTCACCAAGTCCTATGCGTCCAAAGCGGCCTT
TATCGGCTATCGCCATGAATGCGCTTACTTGCTCGCCAAAGGCCGTCCCCAGGTGCCCGCGCAGCCGTTGCCGGATGTGA
TGCCCTGGGCCTACTCAGGTAATCGACACCATCCCACCGAGAAGCCGGTGAGCGCGTTGCAGCCCCTGATAGAGTCATTT
ACCCGTCCGGGTGACATTGTGCTCGACCCGTTCGCAGGCTCAGGCTCTACGTGCGTGGCGGCGGCCAAGTGTGGGCGGCG
CTATATAGGGATCGAGTTGCTGCCGCATTATCACCAGGCAGGGTTAAATCGACTGAATCTGGCCAATGGACTGCGGGCCG
CATAG

Upstream 100 bases:

>100_bases
GCTTGACCCGCTGCGATGCATTGCGTGTTGGCCTCGGGCAGCAGGGAATGGCAACCATCCCGGAGACCTCCTGCCGCCCT
TCTATGACGAGGCCAATACC

Downstream 100 bases:

>100_bases
GGGAGGGGAAGAAGATGCGAGAACTTGAAACACGGTTAGGCGACATGGCAAAGCAAGCCCTTGAACTGCTCAAGACCATG
GAGGCGGTCAGAATGGAGGC

Product: putative methylase

Products: S-Adenosyl-L-Homocysteine; DNA 6-Methylaminopurine. [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSRFMLGDSVQIMSTFPGAAVDFILTDPPYLVGFKDRSGRSIANDVNDEWVLPAFTQMYRVLKPHSLAVSFYGWNRVDTF
MAAWKQAGFRVVGHLVFTKSYASKAAFIGYRHECAYLLAKGRPQVPAQPLPDVMPWAYSGNRHHPTEKPVSALQPLIESF
TRPGDIVLDPFAGSGSTCVAAAKCGRRYIGIELLPHYHQAGLNRLNLANGLRAA

Sequences:

>Translated_214_residues
MSRFMLGDSVQIMSTFPGAAVDFILTDPPYLVGFKDRSGRSIANDVNDEWVLPAFTQMYRVLKPHSLAVSFYGWNRVDTF
MAAWKQAGFRVVGHLVFTKSYASKAAFIGYRHECAYLLAKGRPQVPAQPLPDVMPWAYSGNRHHPTEKPVSALQPLIESF
TRPGDIVLDPFAGSGSTCVAAAKCGRRYIGIELLPHYHQAGLNRLNLANGLRAA
>Mature_213_residues
SRFMLGDSVQIMSTFPGAAVDFILTDPPYLVGFKDRSGRSIANDVNDEWVLPAFTQMYRVLKPHSLAVSFYGWNRVDTFM
AAWKQAGFRVVGHLVFTKSYASKAAFIGYRHECAYLLAKGRPQVPAQPLPDVMPWAYSGNRHHPTEKPVSALQPLIESFT
RPGDIVLDPFAGSGSTCVAAAKCGRRYIGIELLPHYHQAGLNRLNLANGLRAA

Specific function: Unknown

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=254, Percent_Identity=28.3464566929134, Blast_Score=81, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: 2.1.1.72 [C]

Molecular weight: Translated: 23657; Mature: 23526

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFMLGDSVQIMSTFPGAAVDFILTDPPYLVGFKDRSGRSIANDVNDEWVLPAFTQMYR
CCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEECCCCCCCHHHCCCCCEECHHHHHHHH
VLKPHSLAVSFYGWNRVDTFMAAWKQAGFRVVGHLVFTKSYASKAAFIGYRHECAYLLAK
HHCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
GRPQVPAQPLPDVMPWAYSGNRHHPTEKPVSALQPLIESFTRPGDIVLDPFAGSGSTCVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHH
AAKCGRRYIGIELLPHYHQAGLNRLNLANGLRAA
HHHCCCEEEEEEECCCHHHHCCCHHHHCCCCCCC
>Mature Secondary Structure 
SRFMLGDSVQIMSTFPGAAVDFILTDPPYLVGFKDRSGRSIANDVNDEWVLPAFTQMYR
CCCCCCCCEEEEECCCCCEEEEEECCCCEEEEECCCCCCCHHHCCCCCEECHHHHHHHH
VLKPHSLAVSFYGWNRVDTFMAAWKQAGFRVVGHLVFTKSYASKAAFIGYRHECAYLLAK
HHCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
GRPQVPAQPLPDVMPWAYSGNRHHPTEKPVSALQPLIESFTRPGDIVLDPFAGSGSTCVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHH
AAKCGRRYIGIELLPHYHQAGLNRLNLANGLRAA
HHHCCCEEEEEEECCCHHHHCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; DNA Adenine; DNA [C]

Specific reaction: S-Adenosyl-L-Methionine + DNA Adenine = S-Adenosyl-L-Homocysteine + DNA 6-Methylaminopurine. Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10366527 [H]