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The map label for this gene is patB [H]

Identifier: 145300018

GI number: 145300018

Start: 3366986

End: 3368125

Strand: Direct

Name: patB [H]

Synonym: ASA_3117

Alternate gene names: 145300018

Gene position: 3366986-3368125 (Clockwise)

Preceding gene: 145300016

Following gene: 145300019

Centisome position: 71.6

GC content: 63.51

Gene sequence:

>1140_bases
ATGTTTGATTTTGACCGGGAAATAGATCGTCGCGACACCATGAGCCTGAAATGGGACAAGTACAAAGGCGAGGACGTGCT
GCCCATGTGGGTTGCCGACACCGACTTTGTCTCGCCGCCCGCCGTCGTCGAGGCCCTCACCAAGCGGGTCGCCCACGGTA
TCTTCGGTTACTCGCGCCCCTCTGCCCGGCTGATCGAACTCATCATGGCGCGTCTGGCCAGCCGCTACGGCTGGACCATA
CAGCCAGAGTGGCTCATCTTCCTGCCCGGCGTGGTACCAGGTCTGAACCTGGCGTGCAAAGCCTGGAGCCAGCAAGGCCG
CGGCATCATCACCCCCAAGCCGGTCTACTACCCCTTCCTGCAAGCCCCCGGTTTCAACGACCGTCCCCTGCTCACAGTCC
CCATGGTGGAAGAGGCGGGTCGCTGGGTGCTGGATCTGGAAGCGCTGGAACGTCAGGCCCCGTTTGGTGATCTGCTGCTG
CTGTGCAACCCGCACAACCCGGGTGGGACAGTGTTTACCCGCGAGGAGCTCGAAGCCATCGATGCCATCGCCGAGCGCCA
CAATCTGGTGGTCTGCTCGGACGAGATCCATTGCGATCTGCTGCTGGACAAAAATGCCCGTCACATACCCTATGGCGCCG
TCAGCCCGGAAGCCGCCGATCGCTGCGCCGTCATGATGGCACCGAGCAAGACCTTCAATATTGCGGGTCTGTGCTGCGCC
TTCGCCGTGGTGCCCAACGCCCGCCTGCGCCTCAAGCTGCAACAGGCAATGCGCGGCATCAGCGCCGACGTCAACCTGCT
GGGCTTTGTCGCGGCCGAAGCGGCCTATGAAGGGGGCGACCCCTGGCTTGCCGAACAGCTCGACTATCTGGCTGCCAATC
TGGCGCTGATCGAGCAGGCGGTCGCCCGCTGGCCTGGCGTCACGCTGGCGCGCCATCAGGCGACCTACCTCGCCTGGATC
GATGTCAGTGCGCTCGGGCTCGACGATCCCATCGCCTTCTTCGAACAGGCGGAAGTGGGACTCTCCCCCGGCGCCCAGTT
CGGCAATGGCCAGTTCGTGCGGCTCAACTTCGGTTGCACCAGAGCACGGCTGACCGAAGCACTGGCTCGGATGGAGAAAG
CGATCCAGACTCGCATCTGA

Upstream 100 bases:

>100_bases
GCCGCTTATTTTATGTCATTATCAAGAGTTACCTCAAGGCTTGCGACCCCGCGCCCGACCCCGTACATTGAAAGCCCACT
GGCCAGTATAAGGATTTCAC

Downstream 100 bases:

>100_bases
CGGCAATAGCGTATATTTTTGCAGCTGCATGTCGGCGGCAACCAACTACGCTTGAGCGCAGTCGTTGCGGGCCACAATGG
TTGACTCCATTGTGGCCCGC

Product: aminotransferase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MFDFDREIDRRDTMSLKWDKYKGEDVLPMWVADTDFVSPPAVVEALTKRVAHGIFGYSRPSARLIELIMARLASRYGWTI
QPEWLIFLPGVVPGLNLACKAWSQQGRGIITPKPVYYPFLQAPGFNDRPLLTVPMVEEAGRWVLDLEALERQAPFGDLLL
LCNPHNPGGTVFTREELEAIDAIAERHNLVVCSDEIHCDLLLDKNARHIPYGAVSPEAADRCAVMMAPSKTFNIAGLCCA
FAVVPNARLRLKLQQAMRGISADVNLLGFVAAEAAYEGGDPWLAEQLDYLAANLALIEQAVARWPGVTLARHQATYLAWI
DVSALGLDDPIAFFEQAEVGLSPGAQFGNGQFVRLNFGCTRARLTEALARMEKAIQTRI

Sequences:

>Translated_379_residues
MFDFDREIDRRDTMSLKWDKYKGEDVLPMWVADTDFVSPPAVVEALTKRVAHGIFGYSRPSARLIELIMARLASRYGWTI
QPEWLIFLPGVVPGLNLACKAWSQQGRGIITPKPVYYPFLQAPGFNDRPLLTVPMVEEAGRWVLDLEALERQAPFGDLLL
LCNPHNPGGTVFTREELEAIDAIAERHNLVVCSDEIHCDLLLDKNARHIPYGAVSPEAADRCAVMMAPSKTFNIAGLCCA
FAVVPNARLRLKLQQAMRGISADVNLLGFVAAEAAYEGGDPWLAEQLDYLAANLALIEQAVARWPGVTLARHQATYLAWI
DVSALGLDDPIAFFEQAEVGLSPGAQFGNGQFVRLNFGCTRARLTEALARMEKAIQTRI
>Mature_379_residues
MFDFDREIDRRDTMSLKWDKYKGEDVLPMWVADTDFVSPPAVVEALTKRVAHGIFGYSRPSARLIELIMARLASRYGWTI
QPEWLIFLPGVVPGLNLACKAWSQQGRGIITPKPVYYPFLQAPGFNDRPLLTVPMVEEAGRWVLDLEALERQAPFGDLLL
LCNPHNPGGTVFTREELEAIDAIAERHNLVVCSDEIHCDLLLDKNARHIPYGAVSPEAADRCAVMMAPSKTFNIAGLCCA
FAVVPNARLRLKLQQAMRGISADVNLLGFVAAEAAYEGGDPWLAEQLDYLAANLALIEQAVARWPGVTLARHQATYLAWI
DVSALGLDDPIAFFEQAEVGLSPGAQFGNGQFVRLNFGCTRARLTEALARMEKAIQTRI

Specific function: Catalyzes the transformation of cystathionine to homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine [H]

COG id: COG1168

COG function: function code E; Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787909, Length=391, Percent_Identity=33.7595907928389, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788722, Length=373, Percent_Identity=24.6648793565684, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994476, Length=203, Percent_Identity=27.0935960591133, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71994472, Length=202, Percent_Identity=26.2376237623762, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 41965; Mature: 41965

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDFDREIDRRDTMSLKWDKYKGEDVLPMWVADTDFVSPPAVVEALTKRVAHGIFGYSRP
CCCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCC
SARLIELIMARLASRYGWTIQPEWLIFLPGVVPGLNLACKAWSQQGRGIITPKPVYYPFL
HHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEE
QAPGFNDRPLLTVPMVEEAGRWVLDLEALERQAPFGDLLLLCNPHNPGGTVFTREELEAI
CCCCCCCCCEEEECCHHHCCCEEEEHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHH
DAIAERHNLVVCSDEIHCDLLLDKNARHIPYGAVSPEAADRCAVMMAPSKTFNIAGLCCA
HHHHHHCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHH
FAVVPNARLRLKLQQAMRGISADVNLLGFVAAEAAYEGGDPWLAEQLDYLAANLALIEQA
HHHCCCCCEEEEHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VARWPGVTLARHQATYLAWIDVSALGLDDPIAFFEQAEVGLSPGAQFGNGQFVRLNFGCT
HHHCCCCEEECCCEEEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCC
RARLTEALARMEKAIQTRI
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFDFDREIDRRDTMSLKWDKYKGEDVLPMWVADTDFVSPPAVVEALTKRVAHGIFGYSRP
CCCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCC
SARLIELIMARLASRYGWTIQPEWLIFLPGVVPGLNLACKAWSQQGRGIITPKPVYYPFL
HHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEE
QAPGFNDRPLLTVPMVEEAGRWVLDLEALERQAPFGDLLLLCNPHNPGGTVFTREELEAI
CCCCCCCCCEEEECCHHHCCCEEEEHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHH
DAIAERHNLVVCSDEIHCDLLLDKNARHIPYGAVSPEAADRCAVMMAPSKTFNIAGLCCA
HHHHHHCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHH
FAVVPNARLRLKLQQAMRGISADVNLLGFVAAEAAYEGGDPWLAEQLDYLAANLALIEQA
HHHCCCCCEEEEHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VARWPGVTLARHQATYLAWIDVSALGLDDPIAFFEQAEVGLSPGAQFGNGQFVRLNFGCT
HHHCCCCEEECCCEEEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCC
RARLTEALARMEKAIQTRI
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]