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The map label for this gene is ptsH [H]

Identifier: 145299966

GI number: 145299966

Start: 3295021

End: 3295278

Strand: Direct

Name: ptsH [H]

Synonym: ASA_3060

Alternate gene names: 145299966

Gene position: 3295021-3295278 (Clockwise)

Preceding gene: 145299965

Following gene: 145299967

Centisome position: 70.07

GC content: 56.98

Gene sequence:

>258_bases
ATGTACGAGAAGTCTGTTGTTATCACTGCTGAAAACGGCCTGCACACCCGTCCGGCAGCTCAGTTCGTGAAAGAAGCCAA
AGAATTCCAAAGCGAGATCACTGTGGTCTCCGGTGGCAAATCCGCCAGCGCCAAGAGCCTGTTCAAGCTGCAGACCCTGG
GCCTGACCAAAGGCACCAACGTGACCATCCAGGCCGACGGCCCGGACGCTCAGAAAGCCGTTGAGAAGCTGGTTGCCCTG
ATGGACGAGCTGGAGTAA

Upstream 100 bases:

>100_bases
ATTTCGTTACTTTACAACATATCCATACTGACACGGTGAGACAAGGAAGCGTCAAAAGCATCCATCTCTCCAAAAAATAT
CAATAATATGGGGTGAAACC

Downstream 100 bases:

>100_bases
TCCAGCTTTATTCCTCCCCCACAGTCGTTTTAATAGGAAGGATTATGATATCTGGCATTCTTGCATCCCCCGGTATCGCA
TTCGGTAAGGCGCTTCTTCT

Product: phosphocarrier protein HPr

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; D-glucosamine-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; galactitol-1-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm]; fructose-1-phosphate [Cytoplasm]; glucose-6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm]; salicin-6-phosphate [Cytoplasm]; arbutin-6-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein [H]

Number of amino acids: Translated: 85; Mature: 85

Protein sequence:

>85_residues
MYEKSVVITAENGLHTRPAAQFVKEAKEFQSEITVVSGGKSASAKSLFKLQTLGLTKGTNVTIQADGPDAQKAVEKLVAL
MDELE

Sequences:

>Translated_85_residues
MYEKSVVITAENGLHTRPAAQFVKEAKEFQSEITVVSGGKSASAKSLFKLQTLGLTKGTNVTIQADGPDAQKAVEKLVAL
MDELE
>Mature_85_residues
MYEKSVVITAENGLHTRPAAQFVKEAKEFQSEITVVSGGKSASAKSLFKLQTLGLTKGTNVTIQADGPDAQKAVEKLVAL
MDELE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

Organism=Escherichia coli, GI1788755, Length=85, Percent_Identity=77.6470588235294, Blast_Score=128, Evalue=9e-32,

Paralogues:

None

Copy number: 4180 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 2100 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 3235 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 9094; Mature: 9094

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEKSVVITAENGLHTRPAAQFVKEAKEFQSEITVVSGGKSASAKSLFKLQTLGLTKGTN
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCCCC
VTIQADGPDAQKAVEKLVALMDELE
EEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYEKSVVITAENGLHTRPAAQFVKEAKEFQSEITVVSGGKSASAKSLFKLQTLGLTKGTN
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCCCC
VTIQADGPDAQKAVEKLVALMDELE
EEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sorbitol [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm]; mannitol [Periplasm]; glucosamine [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; galactitol [Periplasm]; fructose [Periplasm]; beta-D-glucose [Periplasm]; diacetylchitobiose [Periplasm]; cellobiose [Periplasm]; salicin [Periplasm]; arbutin [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + mannitol [Periplasm] = mannitol-1-phosp

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]