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The map label for this gene is ptsI [H]

Identifier: 145299967

GI number: 145299967

Start: 3295323

End: 3297050

Strand: Direct

Name: ptsI [H]

Synonym: ASA_3061

Alternate gene names: 145299967

Gene position: 3295323-3297050 (Clockwise)

Preceding gene: 145299966

Following gene: 145299968

Centisome position: 70.08

GC content: 54.57

Gene sequence:

>1728_bases
ATGATATCTGGCATTCTTGCATCCCCCGGTATCGCATTCGGTAAGGCGCTTCTTCTTAAAGAAGATGAAATCGTTATCAA
TCAGGGCAAGATTTCTGCTGACCAGATTGACGCCGAGATCAACCGCTTCCTCGAAGCCCGCACCAAGTCAGCTGCACAGC
TGGAAGCCATCAAAGCCATGGCTGGTCGCACCTTCGGTGAGGAAAAAGAGGCCATCTTCGAAGGCCACATCATGCTGCTC
GAAGATGAGGAGCTGGAAGGGGATATCAGATCCTTCATCAAAGACAACAAGGCCACCGCTGATAAGGCCGTCTACGAGGT
CATCGAGCAATACGCCAAGATGATGGCCGATCTGGACGATCCCTACCTGCGCGAGCGCGCCACCGACTTCCGTGACATCG
GAACCCGTCTGGTGAAGAACGTGCTGGGCATCGCCGTTGTCAATCTGAGCACCATAGACGAAGAGGTCATCCTGGTCGCC
AAAGACCTGACTCCGTCCGAAACTGCCCAGATCAACCTGAAATATGTGTTGGGTTTCGTTACCGATATCGGTGGCCGTAC
CTCTCACACCTCCATCATGGCGCGCTCCCTTGAGCTGCCGGCCATAGTGGGCACCAACGACATCACCGAGCGCGTCAACA
ATGGCGAGATGCTGATACTGGATGCGATCAACAACCAGATCATCATCAACCCGACCGCTGAACAGCTGAACGAAGCCAAG
CAATTCCAGGCCCAGTTCCAGGCTGAAAAAGATGAGCTGGCCAAGCTGAAAGATCTGCCTGCCATCACCCTGGATGGTCA
CCAGGTCGAAGTCTGCGCCAACATAGGTACCGTCAAGGATACCGAAGGTGCCATCCGCAATGGCGCCGAAGGCGTGGGTC
TGTACCGTACCGAGTTCCTGTTCATGGATCGCGATGCGCTGCCGACCGAAGACGAGCAGTTCAAGGCCTACAAAGAAGTG
GCAGAGGCCATGCCGGATCAGCCGATCATCGTCCGTACCATGGACATTGGCGGCGACAAAGAACTGCCTTACATGAATTT
CCCGAAAGAGATGAACCCCTTCCTGGGCTGGCGTGCCGTGCGTATTTTCTTCGATCGCGAAGACATCATGCACGCCCAGC
TGCGAGCCATACTGCGTGCGTCAGCCTTCGGCAAACTGCGCATCATGTTCCCGATGATCATCTCCGTCGAAGAGTTCCGC
AGCCTCAAGGCCACCGTAGAGAAGCTGAAAGCCGAACTGCGCACCGAAGGCAAAGCCTTTGATGAATCCATCGAAGTGGG
TATCATGATCGAGACTCCAGCTGCTGCCGTGATGGCTCATCATCTGGCTAAAGAAGTGGATTTCTTCAGTATCGGTACCA
ACGACCTGACCCAGTACACTCTGGCAGTCGACCGTGGTAACGAGATGATCTCCGCCATGTACAACCCGCTGTCACCCTCC
GTCCTGACCCTGATCAAGATGGTTATCGACGCTTCCCATGACAATGGCAAGTGGACCGGTATGTGTGGTGAGCTGGCTGG
TGACGAACGCGCCACACTGCTGCTGCTGGGTATGGGTCTGGATGAGTTCTCCATGAGCTCCATCTCTGTACCGCGTATCA
AGAAGCTTATCCGCAACACCAACTTCGAAGATGTGAAGGCAATGGCTGACCAGGCTCTGAGCTATGCAACCGCCGCCGAA
ATCGAAGCCTGTGTAGATAACTTTATTAAGCAGAAGGCAGTCTGCTAA

Upstream 100 bases:

>100_bases
CGGACGCTCAGAAAGCCGTTGAGAAGCTGGTTGCCCTGATGGACGAGCTGGAGTAATCCAGCTTTATTCCTCCCCCACAG
TCGTTTTAATAGGAAGGATT

Downstream 100 bases:

>100_bases
GATCGGCTGCAAACAGCGACAATCATCTCTAGGAGCTTTTACTATGGGTCTGTTTGATAAACTGAAGAAACTGGTTTCCG
ACGATAGCTCTGATACAGGC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MISGILASPGIAFGKALLLKEDEIVINQGKISADQIDAEINRFLEARTKSAAQLEAIKAMAGRTFGEEKEAIFEGHIMLL
EDEELEGDIRSFIKDNKATADKAVYEVIEQYAKMMADLDDPYLRERATDFRDIGTRLVKNVLGIAVVNLSTIDEEVILVA
KDLTPSETAQINLKYVLGFVTDIGGRTSHTSIMARSLELPAIVGTNDITERVNNGEMLILDAINNQIIINPTAEQLNEAK
QFQAQFQAEKDELAKLKDLPAITLDGHQVEVCANIGTVKDTEGAIRNGAEGVGLYRTEFLFMDRDALPTEDEQFKAYKEV
AEAMPDQPIIVRTMDIGGDKELPYMNFPKEMNPFLGWRAVRIFFDREDIMHAQLRAILRASAFGKLRIMFPMIISVEEFR
SLKATVEKLKAELRTEGKAFDESIEVGIMIETPAAAVMAHHLAKEVDFFSIGTNDLTQYTLAVDRGNEMISAMYNPLSPS
VLTLIKMVIDASHDNGKWTGMCGELAGDERATLLLLGMGLDEFSMSSISVPRIKKLIRNTNFEDVKAMADQALSYATAAE
IEACVDNFIKQKAVC

Sequences:

>Translated_575_residues
MISGILASPGIAFGKALLLKEDEIVINQGKISADQIDAEINRFLEARTKSAAQLEAIKAMAGRTFGEEKEAIFEGHIMLL
EDEELEGDIRSFIKDNKATADKAVYEVIEQYAKMMADLDDPYLRERATDFRDIGTRLVKNVLGIAVVNLSTIDEEVILVA
KDLTPSETAQINLKYVLGFVTDIGGRTSHTSIMARSLELPAIVGTNDITERVNNGEMLILDAINNQIIINPTAEQLNEAK
QFQAQFQAEKDELAKLKDLPAITLDGHQVEVCANIGTVKDTEGAIRNGAEGVGLYRTEFLFMDRDALPTEDEQFKAYKEV
AEAMPDQPIIVRTMDIGGDKELPYMNFPKEMNPFLGWRAVRIFFDREDIMHAQLRAILRASAFGKLRIMFPMIISVEEFR
SLKATVEKLKAELRTEGKAFDESIEVGIMIETPAAAVMAHHLAKEVDFFSIGTNDLTQYTLAVDRGNEMISAMYNPLSPS
VLTLIKMVIDASHDNGKWTGMCGELAGDERATLLLLGMGLDEFSMSSISVPRIKKLIRNTNFEDVKAMADQALSYATAAE
IEACVDNFIKQKAVC
>Mature_575_residues
MISGILASPGIAFGKALLLKEDEIVINQGKISADQIDAEINRFLEARTKSAAQLEAIKAMAGRTFGEEKEAIFEGHIMLL
EDEELEGDIRSFIKDNKATADKAVYEVIEQYAKMMADLDDPYLRERATDFRDIGTRLVKNVLGIAVVNLSTIDEEVILVA
KDLTPSETAQINLKYVLGFVTDIGGRTSHTSIMARSLELPAIVGTNDITERVNNGEMLILDAINNQIIINPTAEQLNEAK
QFQAQFQAEKDELAKLKDLPAITLDGHQVEVCANIGTVKDTEGAIRNGAEGVGLYRTEFLFMDRDALPTEDEQFKAYKEV
AEAMPDQPIIVRTMDIGGDKELPYMNFPKEMNPFLGWRAVRIFFDREDIMHAQLRAILRASAFGKLRIMFPMIISVEEFR
SLKATVEKLKAELRTEGKAFDESIEVGIMIETPAAAVMAHHLAKEVDFFSIGTNDLTQYTLAVDRGNEMISAMYNPLSPS
VLTLIKMVIDASHDNGKWTGMCGELAGDERATLLLLGMGLDEFSMSSISVPRIKKLIRNTNFEDVKAMADQALSYATAAE
IEACVDNFIKQKAVC

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=575, Percent_Identity=74.0869565217391, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=584, Percent_Identity=38.1849315068493, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI48994992, Length=500, Percent_Identity=37.4, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1789193, Length=587, Percent_Identity=32.8790459965928, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1787994, Length=449, Percent_Identity=28.5077951002227, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI226510935, Length=193, Percent_Identity=26.9430051813472, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63709; Mature: 63709

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGILASPGIAFGKALLLKEDEIVINQGKISADQIDAEINRFLEARTKSAAQLEAIKAM
CCCCCCCCCCHHHHHEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRTFGEEKEAIFEGHIMLLEDEELEGDIRSFIKDNKATADKAVYEVIEQYAKMMADLDD
HCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
PYLRERATDFRDIGTRLVKNVLGIAVVNLSTIDEEVILVAKDLTPSETAQINLKYVLGFV
HHHHHHCCCHHHHHHHHHHHHHHHEEEEEHHCCCEEEEEEECCCCCCCCEEEHHHHHHHH
TDIGGRTSHTSIMARSLELPAIVGTNDITERVNNGEMLILDAINNQIIINPTAEQLNEAK
HHCCCCCCHHHHHHHHCCCCEEECCCHHHHHCCCCCEEEEEECCCEEEECCCHHHHHHHH
QFQAQFQAEKDELAKLKDLPAITLDGHQVEVCANIGTVKDTEGAIRNGAEGVGLYRTEFL
HHHHHHHCCHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCHHHHCCCCCCCEEEEEEE
FMDRDALPTEDEQFKAYKEVAEAMPDQPIIVRTMDIGGDKELPYMNFPKEMNPFLGWRAV
EEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHCCHHHCEEEE
RIFFDREDIMHAQLRAILRASAFGKLRIMFPMIISVEEFRSLKATVEKLKAELRTEGKAF
EEEECHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DESIEVGIMIETPAAAVMAHHLAKEVDFFSIGTNDLTQYTLAVDRGNEMISAMYNPLSPS
CCCCEEEEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEEECCHHHHHHHHCCCCHH
VLTLIKMVIDASHDNGKWTGMCGELAGDERATLLLLGMGLDEFSMSSISVPRIKKLIRNT
HHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCHHCCCCCCHHHHHHHHHCC
NFEDVKAMADQALSYATAAEIEACVDNFIKQKAVC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISGILASPGIAFGKALLLKEDEIVINQGKISADQIDAEINRFLEARTKSAAQLEAIKAM
CCCCCCCCCCHHHHHEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRTFGEEKEAIFEGHIMLLEDEELEGDIRSFIKDNKATADKAVYEVIEQYAKMMADLDD
HCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
PYLRERATDFRDIGTRLVKNVLGIAVVNLSTIDEEVILVAKDLTPSETAQINLKYVLGFV
HHHHHHCCCHHHHHHHHHHHHHHHEEEEEHHCCCEEEEEEECCCCCCCCEEEHHHHHHHH
TDIGGRTSHTSIMARSLELPAIVGTNDITERVNNGEMLILDAINNQIIINPTAEQLNEAK
HHCCCCCCHHHHHHHHCCCCEEECCCHHHHHCCCCCEEEEEECCCEEEECCCHHHHHHHH
QFQAQFQAEKDELAKLKDLPAITLDGHQVEVCANIGTVKDTEGAIRNGAEGVGLYRTEFL
HHHHHHHCCHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCHHHHCCCCCCCEEEEEEE
FMDRDALPTEDEQFKAYKEVAEAMPDQPIIVRTMDIGGDKELPYMNFPKEMNPFLGWRAV
EEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHCCHHHCEEEE
RIFFDREDIMHAQLRAILRASAFGKLRIMFPMIISVEEFRSLKATVEKLKAELRTEGKAF
EEEECHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DESIEVGIMIETPAAAVMAHHLAKEVDFFSIGTNDLTQYTLAVDRGNEMISAMYNPLSPS
CCCCEEEEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEEECCHHHHHHHHCCCCHH
VLTLIKMVIDASHDNGKWTGMCGELAGDERATLLLLGMGLDEFSMSSISVPRIKKLIRNT
HHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCHHCCCCCCHHHHHHHHHCC
NFEDVKAMADQALSYATAAEIEACVDNFIKQKAVC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2457575; 2960675; 9205837; 9278503; 2411636; 3290198; 9298646; 7876255; 8805571; 9054557; 9541412; 10048929 [H]