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The map label for this gene is dcuB [H]

Identifier: 145299801

GI number: 145299801

Start: 3107410

End: 3108753

Strand: Direct

Name: dcuB [H]

Synonym: ASA_2882

Alternate gene names: 145299801

Gene position: 3107410-3108753 (Clockwise)

Preceding gene: 145299800

Following gene: 145299802

Centisome position: 66.08

GC content: 61.01

Gene sequence:

>1344_bases
ATGATCTTGATGGAATTTATGGTGGTACTCGGCTGCTTGTTGCTGGGTACCCGATTTGGGGGCATGGGACTGGGTCTCAT
CAGTGGCATAGGCCTGTTCCTGCTGACCTTCGTGTTCGGCCTGACACCGGGCGAACCGCCGGTGCAAGTCATGCTGACCA
TACTCGCGGTGATCGGCTGCGCCGCCACCCTGCAGACCGCCGGTGGCCTCAACCTGATGATGCAGGTGGCCGAGCGTCTG
CTGCGCCGTCACCCGCAATACATCACCATACTGGCCCCGCTCACCACCTGGACCCTCACCTTCCTGTGCGGTACCGGCCA
CGTGGTTTACACCATGTTCCCCATCATCTCCGACATAGCGCTGCAAAAGAACATCCGCCCGGAACGGCCGATGGCGGTCG
CCTCCGTCGCCTCCCAGATGGCGATTTGCGCCTCGCCAGTCTCGGTCGCCGTGGTCTCCATGGTCTCCATATTGGCAGCC
GGACACGGTATCGGTGAGGCTTACGGCCTGCTGGATATCCTGATGATTGCCATCCCCTCCTCGTTGACCGGCGTGATGAT
GGCGGCCCTGTGGAGCCTGCGTCGCGGCAAGGATCTGGACAAGGATGAAGAGTTTCAGGCCAGGATCAAGGATCCGGAGC
AGCACGCCTTCATCTATGGCGGCGGTGAAACCCTGCTCAACACCAAGTTCCCGAAAGAGGCCTACTGGTCTACCTGGATC
TTCTTCGCCGCCATCGCTGCCGTGGTATTGCTTGGCGCCAACGAAGGGCTGCGCCCCGTCTTCACCATCAAGGACAAGAC
AGGCCCTCTCTCCATGAACCTGGTGATCCAGATGATGATGCTGATCGCCGGTGCCATCATTCTGATGCGCTGCAAGGTCA
AACCGGGCGAGATCGCCAACGGGGCTGTCTTCAAGGCCGGCATGGTCGCCATCTTCTCCGTGTTCGGGGTTGCATGGATG
AGCGAAACCTTCTTCCAGGCCCACATGCCCCTGCTGAAAGAAACCCTGTCCCACGTGGTACAGGCCCAGCCCTGGACCTA
CGCCCTGGTGCTGTTCCTCATCTCCAAGCTGGTCAACAGCCAGGCTGCCGCCCTGACCGCCATCGCCCCCATGGGTCTGG
CGCTGGGGGTGGAACCCAAGCTGCTGATCGCCTTCCTGCCGGCCAGCTACGGTTACTTCGTGCTGCCGACCTACCCGAGC
GATCTGGCCTGCATCGGTTTCGACCGTTCCGGCACCACCCGCATCGGCAAGTTCATCATCAACCACAGCTTCATCATTCC
GGGGCTGATCGGGGTGAGCAGCTCCTGCGCCCTGGGTTACGTACTGACCAGCATACTGCTGTAA

Upstream 100 bases:

>100_bases
ACAATACCCACTAACCCTTTAGGGCTAAATAAAAACAGACAATCCCGGTCCATGAGCTCAACACAGCACAGATATGGATT
AAATCACGCGAGGTAATGGA

Downstream 100 bases:

>100_bases
TCCCAGCCGCGTTTATCACGCCATTGCCACACAGGCCCCCAACTTCGGGGGCCTGCTCTTTTCTCCCCCTGCCTATTCCC
GTTACGATTTCACGCCACGG

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MILMEFMVVLGCLLLGTRFGGMGLGLISGIGLFLLTFVFGLTPGEPPVQVMLTILAVIGCAATLQTAGGLNLMMQVAERL
LRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIALQKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAA
GHGIGEAYGLLDILMIAIPSSLTGVMMAALWSLRRGKDLDKDEEFQARIKDPEQHAFIYGGGETLLNTKFPKEAYWSTWI
FFAAIAAVVLLGANEGLRPVFTIKDKTGPLSMNLVIQMMMLIAGAIILMRCKVKPGEIANGAVFKAGMVAIFSVFGVAWM
SETFFQAHMPLLKETLSHVVQAQPWTYALVLFLISKLVNSQAAALTAIAPMGLALGVEPKLLIAFLPASYGYFVLPTYPS
DLACIGFDRSGTTRIGKFIINHSFIIPGLIGVSSSCALGYVLTSILL

Sequences:

>Translated_447_residues
MILMEFMVVLGCLLLGTRFGGMGLGLISGIGLFLLTFVFGLTPGEPPVQVMLTILAVIGCAATLQTAGGLNLMMQVAERL
LRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIALQKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAA
GHGIGEAYGLLDILMIAIPSSLTGVMMAALWSLRRGKDLDKDEEFQARIKDPEQHAFIYGGGETLLNTKFPKEAYWSTWI
FFAAIAAVVLLGANEGLRPVFTIKDKTGPLSMNLVIQMMMLIAGAIILMRCKVKPGEIANGAVFKAGMVAIFSVFGVAWM
SETFFQAHMPLLKETLSHVVQAQPWTYALVLFLISKLVNSQAAALTAIAPMGLALGVEPKLLIAFLPASYGYFVLPTYPS
DLACIGFDRSGTTRIGKFIINHSFIIPGLIGVSSSCALGYVLTSILL
>Mature_447_residues
MILMEFMVVLGCLLLGTRFGGMGLGLISGIGLFLLTFVFGLTPGEPPVQVMLTILAVIGCAATLQTAGGLNLMMQVAERL
LRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIALQKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAA
GHGIGEAYGLLDILMIAIPSSLTGVMMAALWSLRRGKDLDKDEEFQARIKDPEQHAFIYGGGETLLNTKFPKEAYWSTWI
FFAAIAAVVLLGANEGLRPVFTIKDKTGPLSMNLVIQMMMLIAGAIILMRCKVKPGEIANGAVFKAGMVAIFSVFGVAWM
SETFFQAHMPLLKETLSHVVQAQPWTYALVLFLISKLVNSQAAALTAIAPMGLALGVEPKLLIAFLPASYGYFVLPTYPS
DLACIGFDRSGTTRIGKFIINHSFIIPGLIGVSSSCALGYVLTSILL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=409, Percent_Identity=56.7237163814181, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1790580, Length=450, Percent_Identity=38.2222222222222, Blast_Score=275, Evalue=5e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 47984; Mature: 47984

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILMEFMVVLGCLLLGTRFGGMGLGLISGIGLFLLTFVFGLTPGEPPVQVMLTILAVIGC
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AATLQTAGGLNLMMQVAERLLRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIA
HHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LQKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAAGHGIGEAYGLLDILMIAIPS
HHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
SLTGVMMAALWSLRRGKDLDKDEEFQARIKDPEQHAFIYGGGETLLNTKFPKEAYWSTWI
HHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCCCEECCCCCHHHHHHHHH
FFAAIAAVVLLGANEGLRPVFTIKDKTGPLSMNLVIQMMMLIAGAIILMRCKVKPGEIAN
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GAVFKAGMVAIFSVFGVAWMSETFFQAHMPLLKETLSHVVQAQPWTYALVLFLISKLVNS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
QAAALTAIAPMGLALGVEPKLLIAFLPASYGYFVLPTYPSDLACIGFDRSGTTRIGKFII
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHH
NHSFIIPGLIGVSSSCALGYVLTSILL
CCCHHHHHHHCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MILMEFMVVLGCLLLGTRFGGMGLGLISGIGLFLLTFVFGLTPGEPPVQVMLTILAVIGC
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AATLQTAGGLNLMMQVAERLLRRHPQYITILAPLTTWTLTFLCGTGHVVYTMFPIISDIA
HHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LQKNIRPERPMAVASVASQMAICASPVSVAVVSMVSILAAGHGIGEAYGLLDILMIAIPS
HHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
SLTGVMMAALWSLRRGKDLDKDEEFQARIKDPEQHAFIYGGGETLLNTKFPKEAYWSTWI
HHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCCCEECCCCCHHHHHHHHH
FFAAIAAVVLLGANEGLRPVFTIKDKTGPLSMNLVIQMMMLIAGAIILMRCKVKPGEIAN
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GAVFKAGMVAIFSVFGVAWMSETFFQAHMPLLKETLSHVVQAQPWTYALVLFLISKLVNS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
QAAALTAIAPMGLALGVEPKLLIAFLPASYGYFVLPTYPSDLACIGFDRSGTTRIGKFII
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHH
NHSFIIPGLIGVSSSCALGYVLTSILL
CCCHHHHHHHCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]