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The map label for this gene is pcaK [H]

Identifier: 145297060

GI number: 145297060

Start: 3279576

End: 3280937

Strand: Reverse

Name: pcaK [H]

Synonym: cgR_2956

Alternate gene names: 145297060

Gene position: 3280937-3279576 (Counterclockwise)

Preceding gene: 145297061

Following gene: 145297059

Centisome position: 99.0

GC content: 52.72

Gene sequence:

>1362_bases
GTGACCGTGACAATTAACCAATCGGTTAGAGGACCAGAGCAAATTCTTGCTGAGGTTCGCTCCAACAAAATGTCTAAGTT
CCAAATCGTTACCGTGACCATTGCCTTGCTGATGGTGGTCCTCGACGGTTTCGAAGTGGGCATCATGGCATTCGCAGCAC
CACAAATCCAAGAACAACTAGGAATCAGCCCAGACATCCTGGGCTATGTACTCAGCGGCAGCCTCTTTGGTATGGCCATC
GGCTCTATCTTCCTCACTCCCCTCGCTGACCGTTTTGGTCGCCGACCCCTCACCTTGGCAATGCTCACACTCATCGTTGT
GGGCATGGCGCTAACGTTGACGGCACCCACAGCACTCTGGCTCATCGTCTGGCGCATCGTCACCGGTTTAGGCATCGGTG
GAATGATGGCAAACCTCAATGCACTTGTTGCGGAGTACTCCTCTGATAAACGACGCACCACAGCGATTGCTATTTACGCA
GCCGGCTACCCTATCGGAGCTACAGTTGCTGGATTCATTGCTAGGCCACTAATTCCACAATATGGATGGCATTCCATGTT
TATCGCTGGAACCATCCTGGCTGTTGTTGCACTCATCGGCGCTTTTGCTTTGCTTCCAGAATCCTTGGATTATCTGCTGG
GTCGTCGACCAAACCGTGCCCTTGACCGTGTCAACAAGATTTTGGGCAAGATGAATGTTGCTCCACTAGACGCACTGCCA
GAACGCACAAATACTGAAGAAGACCGCGTAAGCACTAAAAACACCATCCGCGAAGTTCTTTCCCCACCAACCCTTCGCCT
GACCATGGCATTGTGGCTTGGCTACGCACTCCTTGTTGCCGCATACTACTTTGCTAATACCTGGATCCCCACCATCTTGA
CCAATGTCAGTGGTGACCCACAACTTGGAACCACAATGGGTATTGGCGCAAACCTCGGTGGCGTGTTGGGCTGCTTCGTA
TTCGCAGCTCTTGCCATCCGATTCAGCGGACGCCACCTGCTCTTTATCACCTTGTTTGCCGCAGCCGCCGCATACATCAT
CTTCGGTATGGTCTTCAGCATTATCCCCGTTGCCATCATGGTTGGTTTCGTCCTCGGAGTGCTCACCACCGGTGCTATTG
CAGGTTTCTACGCTGTCGCACCAACCATCTACTCCTCAAAGGCACGCGCTACCGGCATCGGTTGGATGATCGGCATCGGA
CGTCTGGTCTCTGTGGCAGCACCCATCATCGTTGGATACATCCTCGCCGCAGGTGCAGAACCAGAAAAGGTATTCCTGTA
CTTCTCTATCCCGTTGATCATCGCAGGTGTTGCTGCACTGACTCTTTTGCTTGGCCGTACTAAAGCAACACTCGCTGGCT
AA

Upstream 100 bases:

>100_bases
TGATAAGTAAATGCAATCCGGATCACAGTCTGTTTATGATGCTACTCACATTGTTTGGTAGGTGGAAGTTAACTCATAGA
CGAAAGATCAGGAGGTCACC

Downstream 100 bases:

>100_bases
AACTACTTTTAAATTTTTTTTACGGAGGTCACTCATGTCAACCGCCTTTGCCCCTGAATCACCGGAAGCAACCATCACCT
GGAACCCCACACCAGAACAA

Product: hypothetical protein

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTVTINQSVRGPEQILAEVRSNKMSKFQIVTVTIALLMVVLDGFEVGIMAFAAPQIQEQLGISPDILGYVLSGSLFGMAI
GSIFLTPLADRFGRRPLTLAMLTLIVVGMALTLTAPTALWLIVWRIVTGLGIGGMMANLNALVAEYSSDKRRTTAIAIYA
AGYPIGATVAGFIARPLIPQYGWHSMFIAGTILAVVALIGAFALLPESLDYLLGRRPNRALDRVNKILGKMNVAPLDALP
ERTNTEEDRVSTKNTIREVLSPPTLRLTMALWLGYALLVAAYYFANTWIPTILTNVSGDPQLGTTMGIGANLGGVLGCFV
FAALAIRFSGRHLLFITLFAAAAAYIIFGMVFSIIPVAIMVGFVLGVLTTGAIAGFYAVAPTIYSSKARATGIGWMIGIG
RLVSVAAPIIVGYILAAGAEPEKVFLYFSIPLIIAGVAALTLLLGRTKATLAG

Sequences:

>Translated_453_residues
MTVTINQSVRGPEQILAEVRSNKMSKFQIVTVTIALLMVVLDGFEVGIMAFAAPQIQEQLGISPDILGYVLSGSLFGMAI
GSIFLTPLADRFGRRPLTLAMLTLIVVGMALTLTAPTALWLIVWRIVTGLGIGGMMANLNALVAEYSSDKRRTTAIAIYA
AGYPIGATVAGFIARPLIPQYGWHSMFIAGTILAVVALIGAFALLPESLDYLLGRRPNRALDRVNKILGKMNVAPLDALP
ERTNTEEDRVSTKNTIREVLSPPTLRLTMALWLGYALLVAAYYFANTWIPTILTNVSGDPQLGTTMGIGANLGGVLGCFV
FAALAIRFSGRHLLFITLFAAAAAYIIFGMVFSIIPVAIMVGFVLGVLTTGAIAGFYAVAPTIYSSKARATGIGWMIGIG
RLVSVAAPIIVGYILAAGAEPEKVFLYFSIPLIIAGVAALTLLLGRTKATLAG
>Mature_452_residues
TVTINQSVRGPEQILAEVRSNKMSKFQIVTVTIALLMVVLDGFEVGIMAFAAPQIQEQLGISPDILGYVLSGSLFGMAIG
SIFLTPLADRFGRRPLTLAMLTLIVVGMALTLTAPTALWLIVWRIVTGLGIGGMMANLNALVAEYSSDKRRTTAIAIYAA
GYPIGATVAGFIARPLIPQYGWHSMFIAGTILAVVALIGAFALLPESLDYLLGRRPNRALDRVNKILGKMNVAPLDALPE
RTNTEEDRVSTKNTIREVLSPPTLRLTMALWLGYALLVAAYYFANTWIPTILTNVSGDPQLGTTMGIGANLGGVLGCFVF
AALAIRFSGRHLLFITLFAAAAAYIIFGMVFSIIPVAIMVGFVLGVLTTGAIAGFYAVAPTIYSSKARATGIGWMIGIGR
LVSVAAPIIVGYILAAGAEPEKVFLYFSIPLIIAGVAALTLLLGRTKATLAG

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI213021148, Length=477, Percent_Identity=23.4800838574423, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI24308167, Length=423, Percent_Identity=23.1678486997636, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI87081723, Length=426, Percent_Identity=24.6478873239437, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1786229, Length=378, Percent_Identity=24.8677248677249, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87082404, Length=387, Percent_Identity=24.5478036175711, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082231, Length=206, Percent_Identity=27.1844660194175, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87082159, Length=399, Percent_Identity=21.8045112781955, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32564663, Length=435, Percent_Identity=25.2873563218391, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19922874, Length=243, Percent_Identity=23.8683127572016, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48042; Mature: 47911

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTINQSVRGPEQILAEVRSNKMSKFQIVTVTIALLMVVLDGFEVGIMAFAAPQIQEQL
CEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHH
GISPDILGYVLSGSLFGMAIGSIFLTPLADRFGRRPLTLAMLTLIVVGMALTLTAPTALW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LIVWRIVTGLGIGGMMANLNALVAEYSSDKRRTTAIAIYAAGYPIGATVAGFIARPLIPQ
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCC
YGWHSMFIAGTILAVVALIGAFALLPESLDYLLGRRPNRALDRVNKILGKMNVAPLDALP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC
ERTNTEEDRVSTKNTIREVLSPPTLRLTMALWLGYALLVAAYYFANTWIPTILTNVSGDP
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QLGTTMGIGANLGGVLGCFVFAALAIRFSGRHLLFITLFAAAAAYIIFGMVFSIIPVAIM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGFVLGVLTTGAIAGFYAVAPTIYSSKARATGIGWMIGIGRLVSVAAPIIVGYILAAGAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEKVFLYFSIPLIIAGVAALTLLLGRTKATLAG
CCEEEEEEEHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
TVTINQSVRGPEQILAEVRSNKMSKFQIVTVTIALLMVVLDGFEVGIMAFAAPQIQEQL
EEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHH
GISPDILGYVLSGSLFGMAIGSIFLTPLADRFGRRPLTLAMLTLIVVGMALTLTAPTALW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LIVWRIVTGLGIGGMMANLNALVAEYSSDKRRTTAIAIYAAGYPIGATVAGFIARPLIPQ
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCC
YGWHSMFIAGTILAVVALIGAFALLPESLDYLLGRRPNRALDRVNKILGKMNVAPLDALP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC
ERTNTEEDRVSTKNTIREVLSPPTLRLTMALWLGYALLVAAYYFANTWIPTILTNVSGDP
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QLGTTMGIGANLGGVLGCFVFAALAIRFSGRHLLFITLFAAAAAYIIFGMVFSIIPVAIM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGFVLGVLTTGAIAGFYAVAPTIYSSKARATGIGWMIGIGRLVSVAAPIIVGYILAAGAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEKVFLYFSIPLIIAGVAALTLLLGRTKATLAG
CCEEEEEEEHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]