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The map label for this gene is acsA1 [H]

Identifier: 145297059

GI number: 145297059

Start: 3277514

End: 3279541

Strand: Reverse

Name: acsA1 [H]

Synonym: cgR_2955

Alternate gene names: 145297059

Gene position: 3279541-3277514 (Counterclockwise)

Preceding gene: 145297060

Following gene: 145297058

Centisome position: 98.95

GC content: 50.84

Gene sequence:

>2028_bases
ATGTCAACCGCCTTTGCCCCTGAATCACCGGAAGCAACCATCACCTGGAACCCCACACCAGAACAAGTGCGTTCATCAAG
AATGGCTGATTTCGCCCGCTACTCAGAAGTTCCTGCGGGACGTTCACTACCTGATTTCCACTCTTTGCTGGATTGGTCTG
TCACAGAAGTAGAAGCATTCTGGCGTTCTATCCAAAGCTATTTTGATGTCATTGGTGAAGGCTTTGACACTTCTGAAGTA
CTCAAAGAACACGTCATGCCATTTGCATCCTGGTTTCCCGGAGCAAGGGTGAATTTCGCTGAAAATGTTCTCCGACATGC
GCACAACCCCCTGCTTGCCAATGAGCCTGCCATCTACCAAGTAGAAGAAGATGGAAGCCACACGGTGCTGAGCTGGGTTC
AGCTGGAATCCCAAGTGGCGTCCCTTGCTGCAACATTTAAAGAACTTGGTGTTGAGCGCGGAGATCGCATCGCTGCTGTG
CTGCCGAATGTTCCTGAAGCAATCATCGGTCTTCTAGCTGCTGCCAGCATCGGTGCAGTATGGACAATTAACTCTCCAGA
CCTTTCTGCTGAAGCAAGTATCCGCCGAGTAAAGCAGCTGGAACCTAAAGTTCTAGTGGCCAGTGATGGATATGTGTTCG
GTGGTAAAGAAATTGATCGCCGTGAACACACCGCTGAGGTGGAAGCAGGCCTGCCTTCAATTTCCGCAACTGTACTTGTC
CGTATTTTGGATACTCAGCGCGAGCCAGGAGTAGTTTCGGGTAAAGACGTGAAGCGACTTGCCTTCGACGAGTGTGTGAA
CAAGCCACACGCACCTGCATATGAACGCGTTGGTTTCAATGATCCTTTGTGGATTCTCTTTTCTTCCGGCACCACCGGTG
AACCAAAAGGCATTGTTCATGGGCACGGTGGAATGGTCCTTGATGGACTAAAGAATATTTCTCTCCACCAAGACATTGGT
CCCGGCGATGTGTACTACGTTGCCGCGAACACCTCATGGATGGTGTGGAACACCCTGGTGCAAAATCTCCTTGCAGGAGC
TTCAGCAGTGACCTATGCAGGTTCACCAAAAGTCACCGGCAAGGATCATCACTTCCAGATCATTTCTGATTTCGGCGTGA
CGATGTTTGCCACTGGAGCTGCCTATTTAACCATGGTGGAAAAAGCAGGAATTGATCCCCGAGCAGGTCGTGATTTTTCT
GCACTGCGTTCCATTTTGTCTACCGGTTCCCCACTGCCACCAAGCACGTGGCGCTGGGTGCACGATGCCGTGAAATCAGA
TGTGCACTTGGGATCTGATTCTGGCGGAACTGATATTTGCGGTGGATTCCTAGGCTCAAACCCCATGGAGCCGGTTCACC
TGGGCTATCTGCAAGGCCCACTTTTAGGCGTGGCGGTAGAAGCTCACGATGCAGACGGTCAGCCACAAATTGATCAGGTA
GGAGCCATGGCGGTGACAAAGCCGCTTCCCTCCATGCCAGTGATGTTGTGGGGTGATACAGATGGAAGTCGCTACAAGTC
CTCTTATTTCACTGAAGGTGAAGGGATTTGGATGCATGGTGATTGGATCACCCGCACTTCCAGTGGAGAGTTCATTGTTC
ACGGCCGAGCTGATGCCACCCTCAACCGACAAGGTGTGAGAATCGGACCTTCTGATATTTACGATGCACTGACTGATATT
GAAGAACTATCCGACTACCTTGTACTTGGTATTGAAGAGGACAACGGTGGATACTGGATGCCTCTCTTTGTTGTTCTGGA
AGATGGGGTTGAGCTAGATGATGCCCTCCAACAGCGAATTTGTACTACGCTTCGCGCACGCACAAGCGCTCGCCACGTCC
CTGATGAGATCATCGTTGCCCCAGACATTCCAGTCACCCACACCATGAAACGCCTGGAAGTGCCCTTGAAGAAATTCTTT
AGTCCGCAGTGTGCAGGGCGGGAAGTCAACCCTGATTCTGTAAAAAATCCAGACGCCCTCGCCTGGTTTAAGGAATACGC
TCAACAACGATCTGAAGCAAAGGAATAA

Upstream 100 bases:

>100_bases
ATCATCGCAGGTGTTGCTGCACTGACTCTTTTGCTTGGCCGTACTAAAGCAACACTCGCTGGCTAAAACTACTTTTAAAT
TTTTTTTACGGAGGTCACTC

Downstream 100 bases:

>100_bases
AAACTTATGTCACAACAACGAGTGTTGATCACAGCCGGTGCTGGAGGGATTGGTTGGGCTATAGCTCAAGCATTTCTCAA
TACAAACAGCTTGGTGCACA

Product: hypothetical protein

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 675; Mature: 674

Protein sequence:

>675_residues
MSTAFAPESPEATITWNPTPEQVRSSRMADFARYSEVPAGRSLPDFHSLLDWSVTEVEAFWRSIQSYFDVIGEGFDTSEV
LKEHVMPFASWFPGARVNFAENVLRHAHNPLLANEPAIYQVEEDGSHTVLSWVQLESQVASLAATFKELGVERGDRIAAV
LPNVPEAIIGLLAAASIGAVWTINSPDLSAEASIRRVKQLEPKVLVASDGYVFGGKEIDRREHTAEVEAGLPSISATVLV
RILDTQREPGVVSGKDVKRLAFDECVNKPHAPAYERVGFNDPLWILFSSGTTGEPKGIVHGHGGMVLDGLKNISLHQDIG
PGDVYYVAANTSWMVWNTLVQNLLAGASAVTYAGSPKVTGKDHHFQIISDFGVTMFATGAAYLTMVEKAGIDPRAGRDFS
ALRSILSTGSPLPPSTWRWVHDAVKSDVHLGSDSGGTDICGGFLGSNPMEPVHLGYLQGPLLGVAVEAHDADGQPQIDQV
GAMAVTKPLPSMPVMLWGDTDGSRYKSSYFTEGEGIWMHGDWITRTSSGEFIVHGRADATLNRQGVRIGPSDIYDALTDI
EELSDYLVLGIEEDNGGYWMPLFVVLEDGVELDDALQQRICTTLRARTSARHVPDEIIVAPDIPVTHTMKRLEVPLKKFF
SPQCAGREVNPDSVKNPDALAWFKEYAQQRSEAKE

Sequences:

>Translated_675_residues
MSTAFAPESPEATITWNPTPEQVRSSRMADFARYSEVPAGRSLPDFHSLLDWSVTEVEAFWRSIQSYFDVIGEGFDTSEV
LKEHVMPFASWFPGARVNFAENVLRHAHNPLLANEPAIYQVEEDGSHTVLSWVQLESQVASLAATFKELGVERGDRIAAV
LPNVPEAIIGLLAAASIGAVWTINSPDLSAEASIRRVKQLEPKVLVASDGYVFGGKEIDRREHTAEVEAGLPSISATVLV
RILDTQREPGVVSGKDVKRLAFDECVNKPHAPAYERVGFNDPLWILFSSGTTGEPKGIVHGHGGMVLDGLKNISLHQDIG
PGDVYYVAANTSWMVWNTLVQNLLAGASAVTYAGSPKVTGKDHHFQIISDFGVTMFATGAAYLTMVEKAGIDPRAGRDFS
ALRSILSTGSPLPPSTWRWVHDAVKSDVHLGSDSGGTDICGGFLGSNPMEPVHLGYLQGPLLGVAVEAHDADGQPQIDQV
GAMAVTKPLPSMPVMLWGDTDGSRYKSSYFTEGEGIWMHGDWITRTSSGEFIVHGRADATLNRQGVRIGPSDIYDALTDI
EELSDYLVLGIEEDNGGYWMPLFVVLEDGVELDDALQQRICTTLRARTSARHVPDEIIVAPDIPVTHTMKRLEVPLKKFF
SPQCAGREVNPDSVKNPDALAWFKEYAQQRSEAKE
>Mature_674_residues
STAFAPESPEATITWNPTPEQVRSSRMADFARYSEVPAGRSLPDFHSLLDWSVTEVEAFWRSIQSYFDVIGEGFDTSEVL
KEHVMPFASWFPGARVNFAENVLRHAHNPLLANEPAIYQVEEDGSHTVLSWVQLESQVASLAATFKELGVERGDRIAAVL
PNVPEAIIGLLAAASIGAVWTINSPDLSAEASIRRVKQLEPKVLVASDGYVFGGKEIDRREHTAEVEAGLPSISATVLVR
ILDTQREPGVVSGKDVKRLAFDECVNKPHAPAYERVGFNDPLWILFSSGTTGEPKGIVHGHGGMVLDGLKNISLHQDIGP
GDVYYVAANTSWMVWNTLVQNLLAGASAVTYAGSPKVTGKDHHFQIISDFGVTMFATGAAYLTMVEKAGIDPRAGRDFSA
LRSILSTGSPLPPSTWRWVHDAVKSDVHLGSDSGGTDICGGFLGSNPMEPVHLGYLQGPLLGVAVEAHDADGQPQIDQVG
AMAVTKPLPSMPVMLWGDTDGSRYKSSYFTEGEGIWMHGDWITRTSSGEFIVHGRADATLNRQGVRIGPSDIYDALTDIE
ELSDYLVLGIEEDNGGYWMPLFVVLEDGVELDDALQQRICTTLRARTSARHVPDEIIVAPDIPVTHTMKRLEVPLKKFFS
PQCAGREVNPDSVKNPDALAWFKEYAQQRSEAKE

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=672, Percent_Identity=33.3333333333333, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI8923896, Length=673, Percent_Identity=26.8945022288262, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI253970489, Length=686, Percent_Identity=26.3848396501458, Blast_Score=210, Evalue=3e-54,
Organism=Homo sapiens, GI28416953, Length=563, Percent_Identity=26.1101243339254, Blast_Score=202, Evalue=1e-51,
Organism=Homo sapiens, GI13375727, Length=592, Percent_Identity=23.1418918918919, Blast_Score=123, Evalue=6e-28,
Organism=Homo sapiens, GI42544132, Length=553, Percent_Identity=23.1464737793852, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI122937307, Length=547, Percent_Identity=22.6691042047532, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI38505220, Length=553, Percent_Identity=22.9656419529837, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI42544134, Length=358, Percent_Identity=23.7430167597765, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1790505, Length=559, Percent_Identity=26.2969588550984, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI1786529, Length=638, Percent_Identity=23.6677115987461, Blast_Score=140, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17569507, Length=649, Percent_Identity=28.0431432973806, Blast_Score=277, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI71982997, Length=621, Percent_Identity=26.2479871175523, Blast_Score=178, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI71983001, Length=621, Percent_Identity=26.2479871175523, Blast_Score=178, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6323182, Length=588, Percent_Identity=27.2108843537415, Blast_Score=206, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6319264, Length=575, Percent_Identity=25.9130434782609, Blast_Score=183, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24667955, Length=660, Percent_Identity=24.5454545454545, Blast_Score=189, Evalue=5e-48,
Organism=Drosophila melanogaster, GI62472339, Length=529, Percent_Identity=26.0869565217391, Blast_Score=181, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24667959, Length=498, Percent_Identity=25.9036144578313, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI21355225, Length=578, Percent_Identity=26.4705882352941, Blast_Score=160, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 73614; Mature: 73482

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAFAPESPEATITWNPTPEQVRSSRMADFARYSEVPAGRSLPDFHSLLDWSVTEVEAF
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHH
WRSIQSYFDVIGEGFDTSEVLKEHVMPFASWFPGARVNFAENVLRHAHNPLLANEPAIYQ
HHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCEEE
VEEDGSHTVLSWVQLESQVASLAATFKELGVERGDRIAAVLPNVPEAIIGLLAAASIGAV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCEE
WTINSPDLSAEASIRRVKQLEPKVLVASDGYVFGGKEIDRREHTAEVEAGLPSISATVLV
EEECCCCCCHHHHHHHHHHCCCCEEEEECCEEECCCHHHHHHHHHHHHCCCCCHHHHHHH
RILDTQREPGVVSGKDVKRLAFDECVNKPHAPAYERVGFNDPLWILFSSGTTGEPKGIVH
HHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCEEE
GHGGMVLDGLKNISLHQDIGPGDVYYVAANTSWMVWNTLVQNLLAGASAVTYAGSPKVTG
CCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCC
KDHHFQIISDFGVTMFATGAAYLTMVEKAGIDPRAGRDFSALRSILSTGSPLPPSTWRWV
CCCCEEEEHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHH
HDAVKSDVHLGSDSGGTDICGGFLGSNPMEPVHLGYLQGPLLGVAVEAHDADGQPQIDQV
HHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCEEECHHCCCEEEEEEEECCCCCCCCHHHH
GAMAVTKPLPSMPVMLWGDTDGSRYKSSYFTEGEGIWMHGDWITRTSSGEFIVHGRADAT
CCHHCCCCCCCCCEEEEECCCCCHHHHCCEECCCEEEEECCEEEECCCCCEEEECCCCCC
LNRQGVRIGPSDIYDALTDIEELSDYLVLGIEEDNGGYWMPLFVVLEDGVELDDALQQRI
CCCCCCEECHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEEEECCCCHHHHHHHHH
CTTLRARTSARHVPDEIIVAPDIPVTHTMKRLEVPLKKFFSPQCAGREVNPDSVKNPDAL
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
AWFKEYAQQRSEAKE
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
STAFAPESPEATITWNPTPEQVRSSRMADFARYSEVPAGRSLPDFHSLLDWSVTEVEAF
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHH
WRSIQSYFDVIGEGFDTSEVLKEHVMPFASWFPGARVNFAENVLRHAHNPLLANEPAIYQ
HHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCEEE
VEEDGSHTVLSWVQLESQVASLAATFKELGVERGDRIAAVLPNVPEAIIGLLAAASIGAV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCEE
WTINSPDLSAEASIRRVKQLEPKVLVASDGYVFGGKEIDRREHTAEVEAGLPSISATVLV
EEECCCCCCHHHHHHHHHHCCCCEEEEECCEEECCCHHHHHHHHHHHHCCCCCHHHHHHH
RILDTQREPGVVSGKDVKRLAFDECVNKPHAPAYERVGFNDPLWILFSSGTTGEPKGIVH
HHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCEEE
GHGGMVLDGLKNISLHQDIGPGDVYYVAANTSWMVWNTLVQNLLAGASAVTYAGSPKVTG
CCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCC
KDHHFQIISDFGVTMFATGAAYLTMVEKAGIDPRAGRDFSALRSILSTGSPLPPSTWRWV
CCCCEEEEHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHH
HDAVKSDVHLGSDSGGTDICGGFLGSNPMEPVHLGYLQGPLLGVAVEAHDADGQPQIDQV
HHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCEEECHHCCCEEEEEEEECCCCCCCCHHHH
GAMAVTKPLPSMPVMLWGDTDGSRYKSSYFTEGEGIWMHGDWITRTSSGEFIVHGRADAT
CCHHCCCCCCCCCEEEEECCCCCHHHHCCEECCCEEEEECCEEEECCCCCEEEECCCCCC
LNRQGVRIGPSDIYDALTDIEELSDYLVLGIEEDNGGYWMPLFVVLEDGVELDDALQQRI
CCCCCCEECHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEEEECCCCHHHHHHHHH
CTTLRARTSARHVPDEIIVAPDIPVTHTMKRLEVPLKKFFSPQCAGREVNPDSVKNPDAL
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
AWFKEYAQQRSEAKE
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]