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The map label for this gene is thiG

Identifier: 145296005

GI number: 145296005

Start: 2116313

End: 2117095

Strand: Direct

Name: thiG

Synonym: cgR_1929

Alternate gene names: 145296005

Gene position: 2116313-2117095 (Clockwise)

Preceding gene: 145296004

Following gene: 145296006

Centisome position: 63.86

GC content: 59.26

Gene sequence:

>783_bases
ATGCTGCATATTGCTGATAAAACTTTCGATTCCCACCTCATCATGGGCACCGGCGGAGCTACCTCTCAGGCGTTGCTGGA
GGAATCCCTTGTCGCCAGTGGAACTCAATTGACCACCGTGGCGATGCGTCGACACCAAGCAACCACCTCTAGCGGAGAGT
CCATCTTTGCCATGCTGCGCCGTCTCGAGATCGCCCTTCTCCCCAATACCGCAGGCTGCCGCACTGCCCGCGACGCCGTC
CTCACTGCACAATTGGCTCGGGAAGCCCTCGATACCAACTGGGTGAAAGTGGAGGTCATCGCCGATGAACACACATTGCT
GCCCGACACCGTTGAGCTTCTTGATGCCTGCGAACTCCTCGTCCACGACGGCTTCACCGTTCTGGCTTACACCTCTGACG
ATCCCATCACCGCCAGCCGTTTGGAAGACTGTGGCGTTGCAGCCGTCATGCCATTGGGCTCCCCGATCGGAACCGGCCTG
GGCATTCTCAACCCGCACAACATTGAACTGATTTGCAGCCGCGCGAGCGTACCCGTGATCCTCGACGCCGGTGTAGGCAC
TGCCTCTGATGCCACACTCGCAATGGAACTCGGCTGCGACGGCGTCCTTCTGGCCTCCGCTATCAACCGCGCCCAAAATC
CCGTCGCGATGGCCAAATCCATGTTCCACGCCGTTGAAGCCGGAAGATTAGCCGCCCAAGCAGGCCGAATCCCGCAACGC
CAACATGCGGTGGCCTCATCAAGTTTTGAAGGTTTGGCATCCTGGGCGGAGCAGGTGCTTTAA

Upstream 100 bases:

>100_bases
GTGGCAATCAACGCCGCGGTCGTACCCAGATCCCAGTGGTCACGCGCCATTTGTGACAACGATTCCGTAGAAGTTCTCAC
CGCAATTCAGGGAGGTTAAA

Downstream 100 bases:

>100_bases
CATGGCGCAGCTTCCCACATCTGAGCTTCACCGCACGGCCCGCCAACTCGCGCTCCCCGGATATGGCATTGAGCAGCAGG
AGCGACTTTTCAACGCCCAC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLHIADKTFDSHLIMGTGGATSQALLEESLVASGTQLTTVAMRRHQATTSSGESIFAMLRRLEIALLPNTAGCRTARDAV
LTAQLAREALDTNWVKVEVIADEHTLLPDTVELLDACELLVHDGFTVLAYTSDDPITASRLEDCGVAAVMPLGSPIGTGL
GILNPHNIELICSRASVPVILDAGVGTASDATLAMELGCDGVLLASAINRAQNPVAMAKSMFHAVEAGRLAAQAGRIPQR
QHAVASSSFEGLASWAEQVL

Sequences:

>Translated_260_residues
MLHIADKTFDSHLIMGTGGATSQALLEESLVASGTQLTTVAMRRHQATTSSGESIFAMLRRLEIALLPNTAGCRTARDAV
LTAQLAREALDTNWVKVEVIADEHTLLPDTVELLDACELLVHDGFTVLAYTSDDPITASRLEDCGVAAVMPLGSPIGTGL
GILNPHNIELICSRASVPVILDAGVGTASDATLAMELGCDGVLLASAINRAQNPVAMAKSMFHAVEAGRLAAQAGRIPQR
QHAVASSSFEGLASWAEQVL
>Mature_260_residues
MLHIADKTFDSHLIMGTGGATSQALLEESLVASGTQLTTVAMRRHQATTSSGESIFAMLRRLEIALLPNTAGCRTARDAV
LTAQLAREALDTNWVKVEVIADEHTLLPDTVELLDACELLVHDGFTVLAYTSDDPITASRLEDCGVAAVMPLGSPIGTGL
GILNPHNIELICSRASVPVILDAGVGTASDATLAMELGCDGVLLASAINRAQNPVAMAKSMFHAVEAGRLAAQAGRIPQR
QHAVASSSFEGLASWAEQVL

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=48.4126984126984, Blast_Score=221, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_CORGB (A4QFA8)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138826.1
- ProteinModelPortal:   A4QFA8
- SMR:   A4QFA8
- STRING:   A4QFA8
- GeneID:   4992570
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1929
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27248; Mature: 27248

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: ACT_SITE 96-96 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHIADKTFDSHLIMGTGGATSQALLEESLVASGTQLTTVAMRRHQATTSSGESIFAMLR
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHH
RLEIALLPNTAGCRTARDAVLTAQLAREALDTNWVKVEVIADEHTLLPDTVELLDACELL
HHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
VHDGFTVLAYTSDDPITASRLEDCGVAAVMPLGSPIGTGLGILNPHNIELICSRASVPVI
HHCCEEEEEECCCCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCEE
LDAGVGTASDATLAMELGCDGVLLASAINRAQNPVAMAKSMFHAVEAGRLAAQAGRIPQR
EECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
QHAVASSSFEGLASWAEQVL
HHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MLHIADKTFDSHLIMGTGGATSQALLEESLVASGTQLTTVAMRRHQATTSSGESIFAMLR
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHH
RLEIALLPNTAGCRTARDAVLTAQLAREALDTNWVKVEVIADEHTLLPDTVELLDACELL
HHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
VHDGFTVLAYTSDDPITASRLEDCGVAAVMPLGSPIGTGLGILNPHNIELICSRASVPVI
HHCCEEEEEECCCCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCEE
LDAGVGTASDATLAMELGCDGVLLASAINRAQNPVAMAKSMFHAVEAGRLAAQAGRIPQR
EECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
QHAVASSSFEGLASWAEQVL
HHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA