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The map label for this gene is moeZ [H]

Identifier: 145296006

GI number: 145296006

Start: 2117037

End: 2118173

Strand: Direct

Name: moeZ [H]

Synonym: cgR_1930

Alternate gene names: 145296006

Gene position: 2117037-2118173 (Clockwise)

Preceding gene: 145296005

Following gene: 145296011

Centisome position: 63.88

GC content: 59.54

Gene sequence:

>1137_bases
ATGCGGTGGCCTCATCAAGTTTTGAAGGTTTGGCATCCTGGGCGGAGCAGGTGCTTTAACATGGCGCAGCTTCCCACATC
TGAGCTTCACCGCACGGCCCGCCAACTCGCGCTCCCCGGATATGGCATTGAGCAGCAGGAGCGACTTTTCAACGCCCACG
TTCTAGTCATCGGCGCAGGCGGTCTCGGCTGCCCAGTCATGCAGTCACTGGCTTCCGCAGGCGTTGGAACCATCACGGTC
ATCGATGATGACACCGTCGACATTTCCAACATTCACCGCCAAATCCTCTTCGGCGCAAGCGATGTCGGTCGACCCAAGGT
CGAGGTTGCCGCCGAGCGCCTCAAAGAACTCCAACCAGACATCACCGTCAACGCGTTGCACGAACGGATCACTCCAGAAA
ACGCCTGCGAGCTGCTCAATTCCGTGGACCTCGTCTTGGACGGCTCCGATTCTTTCTCCACAAAATACTTAGTGTCTGAT
GCCGCCGAAATCACCGGAACTCCCCTCATCTGGGCAACGGTACTGCGCTTTCACGGCGAACTGGCACTCTTCAACTCTGG
CCCCGACCACCGCGGAGTCGGCCTGCGCGACGTCTTCCCCGAACAACCCTCCGCCGATTTCGTCCCCGACTGCGCCACCG
CTGGTGTTCTTGGCGCCACCACAGCCATCATCGGCGCACTCATGGCCACTCACGCCATCGGATTTCTCACAGAAATCGGC
GACGTCCAACCAGGCACAATCCTCTCCTACGACGCATTCCCCGCCACCACGCGCAGATTCCGCGTCTCCGCCGACCCGGC
GCGCCCACTGGTCACCCGCCTCCGCGCCTCCTACGAGGCAGCGCGCACCGATACAACTTCGCTTATCGACGCCACCCTCA
ACGGCTCCCTCACCGCCCTCGATATCCGAGAGGCACATGAAGTTCTGCTCAAAGACCTCCCCGAGGGCGCAACGTCACTG
AAGCTCCCCTTAAGCCACATCTCCTCAGATGACGATGTCCTAGAAGCACTCTCTGCCATCGACGGCGATATTTTGGTCTA
CTGTGCTTCAGGAATCCGCAGTTCCGATTTCATCGACAAGTACTCCCACCTCGGCCACAACTTTGTGAATCTTCCCGGTG
GGGTAAACGCGCTGTAG

Upstream 100 bases:

>100_bases
AACCGCGCCCAAAATCCCGTCGCGATGGCCAAATCCATGTTCCACGCCGTTGAAGCCGGAAGATTAGCCGCCCAAGCAGG
CCGAATCCCGCAACGCCAAC

Downstream 100 bases:

>100_bases
CCGTCTGTTTAATAGGCTCAGGGGCGATTCTGTGAGGCCAATTTCTCGTGCCCCTGGATCACTTTTGCGGTGTTTCACGG
CAGTCTGCTTGATTAGGCCA

Product: thiamine biosynthesis protein ThiF

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MRWPHQVLKVWHPGRSRCFNMAQLPTSELHRTARQLALPGYGIEQQERLFNAHVLVIGAGGLGCPVMQSLASAGVGTITV
IDDDTVDISNIHRQILFGASDVGRPKVEVAAERLKELQPDITVNALHERITPENACELLNSVDLVLDGSDSFSTKYLVSD
AAEITGTPLIWATVLRFHGELALFNSGPDHRGVGLRDVFPEQPSADFVPDCATAGVLGATTAIIGALMATHAIGFLTEIG
DVQPGTILSYDAFPATTRRFRVSADPARPLVTRLRASYEAARTDTTSLIDATLNGSLTALDIREAHEVLLKDLPEGATSL
KLPLSHISSDDDVLEALSAIDGDILVYCASGIRSSDFIDKYSHLGHNFVNLPGGVNAL

Sequences:

>Translated_378_residues
MRWPHQVLKVWHPGRSRCFNMAQLPTSELHRTARQLALPGYGIEQQERLFNAHVLVIGAGGLGCPVMQSLASAGVGTITV
IDDDTVDISNIHRQILFGASDVGRPKVEVAAERLKELQPDITVNALHERITPENACELLNSVDLVLDGSDSFSTKYLVSD
AAEITGTPLIWATVLRFHGELALFNSGPDHRGVGLRDVFPEQPSADFVPDCATAGVLGATTAIIGALMATHAIGFLTEIG
DVQPGTILSYDAFPATTRRFRVSADPARPLVTRLRASYEAARTDTTSLIDATLNGSLTALDIREAHEVLLKDLPEGATSL
KLPLSHISSDDDVLEALSAIDGDILVYCASGIRSSDFIDKYSHLGHNFVNLPGGVNAL
>Mature_378_residues
MRWPHQVLKVWHPGRSRCFNMAQLPTSELHRTARQLALPGYGIEQQERLFNAHVLVIGAGGLGCPVMQSLASAGVGTITV
IDDDTVDISNIHRQILFGASDVGRPKVEVAAERLKELQPDITVNALHERITPENACELLNSVDLVLDGSDSFSTKYLVSD
AAEITGTPLIWATVLRFHGELALFNSGPDHRGVGLRDVFPEQPSADFVPDCATAGVLGATTAIIGALMATHAIGFLTEIG
DVQPGTILSYDAFPATTRRFRVSADPARPLVTRLRASYEAARTDTTSLIDATLNGSLTALDIREAHEVLLKDLPEGATSL
KLPLSHISSDDDVLEALSAIDGDILVYCASGIRSSDFIDKYSHLGHNFVNLPGGVNAL

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=341, Percent_Identity=34.8973607038123, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI38045942, Length=72, Percent_Identity=55.5555555555556, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI38045944, Length=72, Percent_Identity=55.5555555555556, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4885649, Length=149, Percent_Identity=32.2147651006711, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI38045948, Length=147, Percent_Identity=36.0544217687075, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1787048, Length=249, Percent_Identity=33.7349397590361, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87082356, Length=212, Percent_Identity=38.2075471698113, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789177, Length=116, Percent_Identity=35.3448275862069, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17540406, Length=247, Percent_Identity=31.1740890688259, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71984476, Length=118, Percent_Identity=41.5254237288136, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193203301, Length=145, Percent_Identity=31.7241379310345, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI86565433, Length=127, Percent_Identity=36.2204724409449, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI86565431, Length=127, Percent_Identity=36.2204724409449, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6321903, Length=253, Percent_Identity=34.3873517786561, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322639, Length=161, Percent_Identity=31.055900621118, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325323, Length=76, Percent_Identity=38.1578947368421, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321965, Length=69, Percent_Identity=47.8260869565217, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24582879, Length=266, Percent_Identity=37.218045112782, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24660640, Length=143, Percent_Identity=34.2657342657343, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28573937, Length=107, Percent_Identity=34.5794392523364, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20129973, Length=109, Percent_Identity=35.7798165137615, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 40585; Mature: 40585

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWPHQVLKVWHPGRSRCFNMAQLPTSELHRTARQLALPGYGIEQQERLFNAHVLVIGAG
CCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEEECC
GLGCPVMQSLASAGVGTITVIDDDTVDISNIHRQILFGASDVGRPKVEVAAERLKELQPD
CCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
ITVNALHERITPENACELLNSVDLVLDGSDSFSTKYLVSDAAEITGTPLIWATVLRFHGE
CCHHHHHHHCCHHHHHHHHCCCEEEEECCCCCCCHHHHHCHHHHCCCHHHHHHHHHHCCC
LALFNSGPDHRGVGLRDVFPEQPSADFVPDCATAGVLGATTAIIGALMATHAIGFLTEIG
EEEECCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DVQPGTILSYDAFPATTRRFRVSADPARPLVTRLRASYEAARTDTTSLIDATLNGSLTAL
CCCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEE
DIREAHEVLLKDLPEGATSLKLPLSHISSDDDVLEALSAIDGDILVYCASGIRSSDFIDK
EHHHHHHHHHHHCCCCCCEEECCHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
YSHLGHNFVNLPGGVNAL
HHHCCCCEEECCCCCCCC
>Mature Secondary Structure
MRWPHQVLKVWHPGRSRCFNMAQLPTSELHRTARQLALPGYGIEQQERLFNAHVLVIGAG
CCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEEECC
GLGCPVMQSLASAGVGTITVIDDDTVDISNIHRQILFGASDVGRPKVEVAAERLKELQPD
CCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
ITVNALHERITPENACELLNSVDLVLDGSDSFSTKYLVSDAAEITGTPLIWATVLRFHGE
CCHHHHHHHCCHHHHHHHHCCCEEEEECCCCCCCHHHHHCHHHHCCCHHHHHHHHHHCCC
LALFNSGPDHRGVGLRDVFPEQPSADFVPDCATAGVLGATTAIIGALMATHAIGFLTEIG
EEEECCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DVQPGTILSYDAFPATTRRFRVSADPARPLVTRLRASYEAARTDTTSLIDATLNGSLTAL
CCCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEE
DIREAHEVLLKDLPEGATSLKLPLSHISSDDDVLEALSAIDGDILVYCASGIRSSDFIDK
EHHHHHHHHHHHCCCCCCEEECCHHHCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
YSHLGHNFVNLPGGVNAL
HHHCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]