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The map label for this gene is nusA [H]

Identifier: 145295891

GI number: 145295891

Start: 2016407

End: 2017405

Strand: Reverse

Name: nusA [H]

Synonym: cgR_1816

Alternate gene names: 145295891

Gene position: 2017405-2016407 (Counterclockwise)

Preceding gene: 145295892

Following gene: 145295890

Centisome position: 60.87

GC content: 54.55

Gene sequence:

>999_bases
GTGAATATTGATGTCCAGGCTTTAAAAGCCATCGAGTCTGAAAAAGGAATCCCAGTTCCAGACTTGCTGCGCACCATCGC
CTCTGCACTTTTGCATTCGTACATGGATAATCGCGAAACTGTTGCGTCTGCGAACCTGAAACCACGCGTGGACATCGATT
CCACAACTGGCACGGTCAACGTCATCGTCTCAGAATTTGACGAAAACGGAGAGCTCGCTTCCGAATACGACGACACCCCA
TCCAACTTCGGACGAGTCAGCGCCCGCGCTGTTCGCGACGCGATCGTTAAGTCCCTGCGCGAAGCAGAAGCAAGCCGAGC
ATTCGATGCGTACGCAGATTATGAAGGCACCGTTGTGTCCGGCATCGTTCAAGCAGATGCCCGCGCAGCTGAACGCGGAA
TCATCATCGTGCAGCTGGGTACCGAAGCGGACAACCAAGACGGCGTTTTGCTCCCAGCCGAGCAGATCCCTGGCGAAAAG
CTCAAGCACGGCGACCGCGTTAAGTGTTTCGTCGTTGGCGTGGGCAAGGGCAACACTGACATCCAGATCAACCTGTCTCG
TACCCACCCTGAGCTGGTGCGCCGACTGTTTGAACTGGAAATCCCAGAAGTTGCTGACGGATCCGTGGAAATTGTTGCTA
TCTCCCGCGAAGCCGGACACCGCTCCAAGGTTGCTGTTCAAGCCAAGGTGAAGAACCTCAACGCCAAGGGCGCTTGCATT
GGCCCACGTGGACAGCGTGTGTCCAACATCATGCGTGAACTCGGTGGAGAAAAAATCGACATCATCGATTACTCCGAAGA
TCCAGCAACCTTCGTTGGAAATGCACTGGCACCATCCAAGGTTGTCAACGTAGAGGTCACCGATCTTGAAGCTCAAACCG
CGCGCGTAACTGTCCCTGACTACCAGCTTTCACTAGCAATCGGTAAAGAAGGTCAAAACGCCCGCTTGGCTGCCCGCCTG
ACCGGCTGGAAGATCGACATCCACTCTGACATCGATTAA

Upstream 100 bases:

>100_bases
ATTCGCTAAACCTGCGCAGGATGAGACTGCCCTCGCAGAAAGCACATTCGACGAAGCCACCGCGTAAACAGTACGTGGTG
GAAGCTTGAGAGGAAGACAA

Downstream 100 bases:

>100_bases
AAGTCGCTTGAACCGGCATGAAAATCTCGTGCTGGTTTTGGGCTCACAAGGCCATATAGGAACTTTGTAATTAGTTGCAG
GTTCCAATTTTGGGTCAATG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MNIDVQALKAIESEKGIPVPDLLRTIASALLHSYMDNRETVASANLKPRVDIDSTTGTVNVIVSEFDENGELASEYDDTP
SNFGRVSARAVRDAIVKSLREAEASRAFDAYADYEGTVVSGIVQADARAAERGIIIVQLGTEADNQDGVLLPAEQIPGEK
LKHGDRVKCFVVGVGKGNTDIQINLSRTHPELVRRLFELEIPEVADGSVEIVAISREAGHRSKVAVQAKVKNLNAKGACI
GPRGQRVSNIMRELGGEKIDIIDYSEDPATFVGNALAPSKVVNVEVTDLEAQTARVTVPDYQLSLAIGKEGQNARLAARL
TGWKIDIHSDID

Sequences:

>Translated_332_residues
MNIDVQALKAIESEKGIPVPDLLRTIASALLHSYMDNRETVASANLKPRVDIDSTTGTVNVIVSEFDENGELASEYDDTP
SNFGRVSARAVRDAIVKSLREAEASRAFDAYADYEGTVVSGIVQADARAAERGIIIVQLGTEADNQDGVLLPAEQIPGEK
LKHGDRVKCFVVGVGKGNTDIQINLSRTHPELVRRLFELEIPEVADGSVEIVAISREAGHRSKVAVQAKVKNLNAKGACI
GPRGQRVSNIMRELGGEKIDIIDYSEDPATFVGNALAPSKVVNVEVTDLEAQTARVTVPDYQLSLAIGKEGQNARLAARL
TGWKIDIHSDID
>Mature_332_residues
MNIDVQALKAIESEKGIPVPDLLRTIASALLHSYMDNRETVASANLKPRVDIDSTTGTVNVIVSEFDENGELASEYDDTP
SNFGRVSARAVRDAIVKSLREAEASRAFDAYADYEGTVVSGIVQADARAAERGIIIVQLGTEADNQDGVLLPAEQIPGEK
LKHGDRVKCFVVGVGKGNTDIQINLSRTHPELVRRLFELEIPEVADGSVEIVAISREAGHRSKVAVQAKVKNLNAKGACI
GPRGQRVSNIMRELGGEKIDIIDYSEDPATFVGNALAPSKVVNVEVTDLEAQTARVTVPDYQLSLAIGKEGQNARLAARL
TGWKIDIHSDID

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=352, Percent_Identity=31.5340909090909, Blast_Score=177, Evalue=8e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N [H]

EC number: NA

Molecular weight: Translated: 35790; Mature: 35790

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDVQALKAIESEKGIPVPDLLRTIASALLHSYMDNRETVASANLKPRVDIDSTTGTVN
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCEEECCCCCEEE
VIVSEFDENGELASEYDDTPSNFGRVSARAVRDAIVKSLREAEASRAFDAYADYEGTVVS
EEEEECCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
GIVQADARAAERGIIIVQLGTEADNQDGVLLPAEQIPGEKLKHGDRVKCFVVGVGKGNTD
HHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEHHHCCCHHHCCCCCEEEEEEEECCCCCE
IQINLSRTHPELVRRLFELEIPEVADGSVEIVAISREAGHRSKVAVQAKVKNLNAKGACI
EEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCEE
GPRGQRVSNIMRELGGEKIDIIDYSEDPATFVGNALAPSKVVNVEVTDLEAQTARVTVPD
CCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCCCCEEEEEEEECCCCCEEEEECCC
YQLSLAIGKEGQNARLAARLTGWKIDIHSDID
EEEEEEECCCCCCCEEEEEEEEEEEEECCCCC
>Mature Secondary Structure
MNIDVQALKAIESEKGIPVPDLLRTIASALLHSYMDNRETVASANLKPRVDIDSTTGTVN
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCEEECCCCCEEE
VIVSEFDENGELASEYDDTPSNFGRVSARAVRDAIVKSLREAEASRAFDAYADYEGTVVS
EEEEECCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
GIVQADARAAERGIIIVQLGTEADNQDGVLLPAEQIPGEKLKHGDRVKCFVVGVGKGNTD
HHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEHHHCCCHHHCCCCCEEEEEEEECCCCCE
IQINLSRTHPELVRRLFELEIPEVADGSVEIVAISREAGHRSKVAVQAKVKNLNAKGACI
EEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCEE
GPRGQRVSNIMRELGGEKIDIIDYSEDPATFVGNALAPSKVVNVEVTDLEAQTARVTVPD
CCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCCCCEEEEEEEECCCCCEEEEECCC
YQLSLAIGKEGQNARLAARLTGWKIDIHSDID
EEEEEEECCCCCCCEEEEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]