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The map label for this gene is pnp

Identifier: 145295879

GI number: 145295879

Start: 2001955

End: 2004216

Strand: Reverse

Name: pnp

Synonym: cgR_1804

Alternate gene names: 145295879

Gene position: 2004216-2001955 (Counterclockwise)

Preceding gene: 145295880

Following gene: 145295877

Centisome position: 60.47

GC content: 56.94

Gene sequence:

>2262_bases
ATGAGCGATGTAAAGTACTTCGAAGACACCGAATTTGGCCTGATCGAGGCCGTCGCAACCATCGACAACGGTGACTTCGG
AACCCGCACCATCCGTTTTGAAACCGGCCAACTTGCCCGCCAGGCAGATGGTGCAGTGACCACCTACCTCGACGATGACA
CGATGCTGCTGGCAACCACCACCGCATCCAACCAGCCACGCGAGGGCTTTGACTTCTTCCCACTGACCGTGGACGTTGAA
GAGCGTATGTACGCAGCTGGTCGCATCCCTGGCTCTTTCTTCCGTCGTGAGGGCCGCCCATCCACCGAAGCTATCCTGGC
TTGCCGTCTCATCGACCGCCCACTGCGCCCAACCTTTGTTAAGGGCCTGCGCAATGAGGTTCAGATCGTTGTCACCGTCA
TGTCCATGAACCCTGAGGATTACTACGATGTCGTAGCAATCAACGGAGCTTCCGCAGCAACCCGCATCTCCGGACTTCCT
GTCTCCGGCGCTGTCGGTGGCGTTCGCATGGCACTGGTTGTTGATGAAAAGCACCCAGAAGGCCAATGGGTTGCATTCCC
AACCCACGCTCAACATGAGCAGTCCGTATTTGAAATCGTTGTGGCTGGTCGCCTCGTCGAGCGCAAGCGCGGCAACAAGA
CCTTCTCCGACGTCGCAGTGATGATGGTGGAAGCTGGCGCTTCCGAAAACGTTGTCAACCGCGTCAAGGACGGTGCACCA
GCACCAACCGAAAAGATCGTCTCCGACGGCCTTGAAGCAGCTAAGCCATTCATCGACATCCTGTGCCGCGCACAGGAAGG
TCTGGCACAGCGCGTTGGAAACGCAGCCAAGGAATTCCCACTGTTCCCTCCATACACCGACGAGGTGTACTCCGCAGTGG
AGCGCAAGGTATCCAAGAAGCTAGCTTCTTTGCTGACCCTGAAGGCAAAGCAAGAGCGCGACGACGCTACCAACGCCTAC
ATGGAAGAAATCGAAGCCGAACTGCTTCCAAAGTTCGAGGCTTCCTACAGCTCTGCAGCTGAAGCGTCCAAGGAAATCCG
CGCAGCATACAACGCTGTCATGAAGTCCATCGTGCGTCGTATGATCCTCACCGATCACTTCCGCATCGACGGCCGCGGAG
TCACCGACATCCGTGACCTGGCAGTAGAAGTTGAGCTCATCCCACGTGCGCACGGTTCCTCCCTTTTCGAGCGTGGCGAG
ACTCAGATCCTCGGTGTCACCACCCTGGACATGCTCAAGATGGAACAGCAAATCGACTCCCTGGCACCAGGCGATGCGAA
GCGCTACATGCACCACTACAACTTCCCTCCATACTCCACTGGAGAAACCGGTCGCGTGGGCTCACCAAAGCGCCGCGAAA
TCGGCCACGGTGCACTTGCAGAACGCGCAGTTTTGCCGGTAATCCCATCTCGTGAGGAATTCCCATACTCAATCCGTCAG
GTCTCTGAGGCTCTGGGCTCCAACGGCTCCACCTCCATGGGCTCTGTCTGTGCATCCACCCTGTCCCTGTACAACGCTGG
TGTTCCACTGAAGGCACCTGTTGCAGGTATCGCCATGGGACTTGTTTCCGGTGAAATCGACGGCAAGACCGAATACGTTG
CACTGACCGATATCCTCGGCGCAGAAGACGCATTCGGCGACATGGACTTCAAGGTTGCCGGCACCGCAGACTTCATCACC
GCACTTCAGCTGGACACCAAGCTGGACGGCATTCCTTCCAAGGTGCTCTCCGATGCGCTTGAGCAGGCACGCGATGCCCG
ACTGACCATCCTGAACACCATGGCTGATGTCATCAACGGACCTGATGAGATGAGCAAATTCGCTCCTCGCATCACCACCG
TGAAGATCCCAGTGGCAAAGATCGGTGAGCTGATCGGACCAAAGGGTAAGAACATCAACGCTCTTACCGAAGAGACCGGC
GCAAACATCTCCATCGAAGATGACGGCACCGTGTTCATCTCTGCAGCTGACGGCGCATCTGCTGAAGCGGCGATCGAAAA
GATCAACGCTCTGGCGAACCCACAGCTGCCAAAGGTTGGCGAGCGCTTCCTCGGAACCGTCGTCAAGACCACCGCATTCG
GCGCATTCGTGTCCTTGCTCCCAGGTCGCGATGGCCTTGTTCACATCTCCAAGCTTGGTAACGGCAAGCGAGTAGAAAAG
GTCGACGATGTGGTGAAGGTTGGCGAGAAGATTCAGGTCGAAATCGCTGACATCGACAACCGCGGCAAGATCTCCTTGGT
CCCAGTTGCTGAAGAGGACTAA

Upstream 100 bases:

>100_bases
AGAGAACTGGTAAGGTTTTTACCGTTCTAGACCGCAGAAATCTTCGCGGCGACACCGATGATCGCCGAGCAGAACTAAAC
ATGAGGAGACCTACTCGCAT

Downstream 100 bases:

>100_bases
TTAATTCTGGCTAGATCGGGCTAGATCGGGCTAAAAATAAATGTGGCGCTAAAACCTTATTGCGAGGTTTTAGCGCCACC
TTTTTCTATCTGTAGTTGAC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 753; Mature: 752

Protein sequence:

>753_residues
MSDVKYFEDTEFGLIEAVATIDNGDFGTRTIRFETGQLARQADGAVTTYLDDDTMLLATTTASNQPREGFDFFPLTVDVE
ERMYAAGRIPGSFFRREGRPSTEAILACRLIDRPLRPTFVKGLRNEVQIVVTVMSMNPEDYYDVVAINGASAATRISGLP
VSGAVGGVRMALVVDEKHPEGQWVAFPTHAQHEQSVFEIVVAGRLVERKRGNKTFSDVAVMMVEAGASENVVNRVKDGAP
APTEKIVSDGLEAAKPFIDILCRAQEGLAQRVGNAAKEFPLFPPYTDEVYSAVERKVSKKLASLLTLKAKQERDDATNAY
MEEIEAELLPKFEASYSSAAEASKEIRAAYNAVMKSIVRRMILTDHFRIDGRGVTDIRDLAVEVELIPRAHGSSLFERGE
TQILGVTTLDMLKMEQQIDSLAPGDAKRYMHHYNFPPYSTGETGRVGSPKRREIGHGALAERAVLPVIPSREEFPYSIRQ
VSEALGSNGSTSMGSVCASTLSLYNAGVPLKAPVAGIAMGLVSGEIDGKTEYVALTDILGAEDAFGDMDFKVAGTADFIT
ALQLDTKLDGIPSKVLSDALEQARDARLTILNTMADVINGPDEMSKFAPRITTVKIPVAKIGELIGPKGKNINALTEETG
ANISIEDDGTVFISAADGASAEAAIEKINALANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGNGKRVEK
VDDVVKVGEKIQVEIADIDNRGKISLVPVAEED

Sequences:

>Translated_753_residues
MSDVKYFEDTEFGLIEAVATIDNGDFGTRTIRFETGQLARQADGAVTTYLDDDTMLLATTTASNQPREGFDFFPLTVDVE
ERMYAAGRIPGSFFRREGRPSTEAILACRLIDRPLRPTFVKGLRNEVQIVVTVMSMNPEDYYDVVAINGASAATRISGLP
VSGAVGGVRMALVVDEKHPEGQWVAFPTHAQHEQSVFEIVVAGRLVERKRGNKTFSDVAVMMVEAGASENVVNRVKDGAP
APTEKIVSDGLEAAKPFIDILCRAQEGLAQRVGNAAKEFPLFPPYTDEVYSAVERKVSKKLASLLTLKAKQERDDATNAY
MEEIEAELLPKFEASYSSAAEASKEIRAAYNAVMKSIVRRMILTDHFRIDGRGVTDIRDLAVEVELIPRAHGSSLFERGE
TQILGVTTLDMLKMEQQIDSLAPGDAKRYMHHYNFPPYSTGETGRVGSPKRREIGHGALAERAVLPVIPSREEFPYSIRQ
VSEALGSNGSTSMGSVCASTLSLYNAGVPLKAPVAGIAMGLVSGEIDGKTEYVALTDILGAEDAFGDMDFKVAGTADFIT
ALQLDTKLDGIPSKVLSDALEQARDARLTILNTMADVINGPDEMSKFAPRITTVKIPVAKIGELIGPKGKNINALTEETG
ANISIEDDGTVFISAADGASAEAAIEKINALANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGNGKRVEK
VDDVVKVGEKIQVEIADIDNRGKISLVPVAEED
>Mature_752_residues
SDVKYFEDTEFGLIEAVATIDNGDFGTRTIRFETGQLARQADGAVTTYLDDDTMLLATTTASNQPREGFDFFPLTVDVEE
RMYAAGRIPGSFFRREGRPSTEAILACRLIDRPLRPTFVKGLRNEVQIVVTVMSMNPEDYYDVVAINGASAATRISGLPV
SGAVGGVRMALVVDEKHPEGQWVAFPTHAQHEQSVFEIVVAGRLVERKRGNKTFSDVAVMMVEAGASENVVNRVKDGAPA
PTEKIVSDGLEAAKPFIDILCRAQEGLAQRVGNAAKEFPLFPPYTDEVYSAVERKVSKKLASLLTLKAKQERDDATNAYM
EEIEAELLPKFEASYSSAAEASKEIRAAYNAVMKSIVRRMILTDHFRIDGRGVTDIRDLAVEVELIPRAHGSSLFERGET
QILGVTTLDMLKMEQQIDSLAPGDAKRYMHHYNFPPYSTGETGRVGSPKRREIGHGALAERAVLPVIPSREEFPYSIRQV
SEALGSNGSTSMGSVCASTLSLYNAGVPLKAPVAGIAMGLVSGEIDGKTEYVALTDILGAEDAFGDMDFKVAGTADFITA
LQLDTKLDGIPSKVLSDALEQARDARLTILNTMADVINGPDEMSKFAPRITTVKIPVAKIGELIGPKGKNINALTEETGA
NISIEDDGTVFISAADGASAEAAIEKINALANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGNGKRVEKV
DDVVKVGEKIQVEIADIDNRGKISLVPVAEED

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=729, Percent_Identity=34.8422496570645, Blast_Score=385, Evalue=1e-107,
Organism=Escherichia coli, GI145693187, Length=728, Percent_Identity=43.8186813186813, Blast_Score=535, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI115534063, Length=747, Percent_Identity=29.9866131191432, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI281362905, Length=722, Percent_Identity=34.6260387811634, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24651641, Length=722, Percent_Identity=34.6260387811634, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24651643, Length=722, Percent_Identity=34.6260387811634, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI161079377, Length=667, Percent_Identity=34.4827586206897, Blast_Score=345, Evalue=5e-95,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_CORGB (A4QEY2)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138700.1
- ProteinModelPortal:   A4QEY2
- SMR:   A4QEY2
- STRING:   A4QEY2
- GeneID:   4991706
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1804
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR014069
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591
- TIGRFAMs:   TIGR02696

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 81290; Mature: 81159

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVKYFEDTEFGLIEAVATIDNGDFGTRTIRFETGQLARQADGAVTTYLDDDTMLLATT
CCCCCCCCCCCCHHHHHHEECCCCCCCCEEEEEECHHHHHHCCCCEEEEECCCEEEEEEE
TASNQPREGFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTEAILACRLIDRPLRPTFV
CCCCCCCCCCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCHHHH
KGLRNEVQIVVTVMSMNPEDYYDVVAINGASAATRISGLPVSGAVGGVRMALVVDEKHPE
HHHCCCEEEEEEEEECCCCCCEEEEEECCCCHHHHCCCCCCCCCCCCEEEEEEEECCCCC
GQWVAFPTHAQHEQSVFEIVVAGRLVERKRGNKTFSDVAVMMVEAGASENVVNRVKDGAP
CCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCC
APTEKIVSDGLEAAKPFIDILCRAQEGLAQRVGNAAKEFPLFPPYTDEVYSAVERKVSKK
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHH
LASLLTLKAKQERDDATNAYMEEIEAELLPKFEASYSSAAEASKEIRAAYNAVMKSIVRR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MILTDHFRIDGRGVTDIRDLAVEVELIPRAHGSSLFERGETQILGVTTLDMLKMEQQIDS
HHHHHCEEECCCCCCHHHHHEEEEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHHH
LAPGDAKRYMHHYNFPPYSTGETGRVGSPKRREIGHGALAERAVLPVIPSREEFPYSIRQ
CCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHCCCCCCCCCCCCCCHHHHH
VSEALGSNGSTSMGSVCASTLSLYNAGVPLKAPVAGIAMGLVSGEIDGKTEYVALTDILG
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEHHHC
AEDAFGDMDFKVAGTADFITALQLDTKLDGIPSKVLSDALEQARDARLTILNTMADVING
CCCCCCCCCEEEECCHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDEMSKFAPRITTVKIPVAKIGELIGPKGKNINALTEETGANISIEDDGTVFISAADGAS
HHHHHHHCCCEEEEEECHHHHHHHHCCCCCCCCHHHHCCCCEEEEECCCEEEEEECCCCC
AEAAIEKINALANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGNGKRVEK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHH
VDDVVKVGEKIQVEIADIDNRGKISLVPVAEED
HHHHHHCCCEEEEEEEECCCCCCEEEEECCCCC
>Mature Secondary Structure 
SDVKYFEDTEFGLIEAVATIDNGDFGTRTIRFETGQLARQADGAVTTYLDDDTMLLATT
CCCCCCCCCCCHHHHHHEECCCCCCCCEEEEEECHHHHHHCCCCEEEEECCCEEEEEEE
TASNQPREGFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTEAILACRLIDRPLRPTFV
CCCCCCCCCCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCHHHH
KGLRNEVQIVVTVMSMNPEDYYDVVAINGASAATRISGLPVSGAVGGVRMALVVDEKHPE
HHHCCCEEEEEEEEECCCCCCEEEEEECCCCHHHHCCCCCCCCCCCCEEEEEEEECCCCC
GQWVAFPTHAQHEQSVFEIVVAGRLVERKRGNKTFSDVAVMMVEAGASENVVNRVKDGAP
CCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCC
APTEKIVSDGLEAAKPFIDILCRAQEGLAQRVGNAAKEFPLFPPYTDEVYSAVERKVSKK
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHH
LASLLTLKAKQERDDATNAYMEEIEAELLPKFEASYSSAAEASKEIRAAYNAVMKSIVRR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MILTDHFRIDGRGVTDIRDLAVEVELIPRAHGSSLFERGETQILGVTTLDMLKMEQQIDS
HHHHHCEEECCCCCCHHHHHEEEEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHHH
LAPGDAKRYMHHYNFPPYSTGETGRVGSPKRREIGHGALAERAVLPVIPSREEFPYSIRQ
CCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHCCCCCCCCCCCCCCHHHHH
VSEALGSNGSTSMGSVCASTLSLYNAGVPLKAPVAGIAMGLVSGEIDGKTEYVALTDILG
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEHHHC
AEDAFGDMDFKVAGTADFITALQLDTKLDGIPSKVLSDALEQARDARLTILNTMADVING
CCCCCCCCCEEEECCHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDEMSKFAPRITTVKIPVAKIGELIGPKGKNINALTEETGANISIEDDGTVFISAADGAS
HHHHHHHCCCEEEEEECHHHHHHHHCCCCCCCCHHHHCCCCEEEEECCCEEEEEECCCCC
AEAAIEKINALANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGNGKRVEK
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHH
VDDVVKVGEKIQVEIADIDNRGKISLVPVAEED
HHHHHHCCCEEEEEEEECCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA