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The map label for this gene is miaB

Identifier: 145295854

GI number: 145295854

Start: 1976927

End: 1978507

Strand: Reverse

Name: miaB

Synonym: cgR_1779

Alternate gene names: 145295854

Gene position: 1978507-1976927 (Counterclockwise)

Preceding gene: 145295859

Following gene: 145295853

Centisome position: 59.7

GC content: 55.34

Gene sequence:

>1581_bases
GTGACGCAGCAATTGAACCACGCAAAGGTAAATCAACATCCCGGTCAAGCCACCCTCCCAGAAACCGCAGAGGGGCAGGT
TCGTACCTACGAGGTAAAAACCTACGGCTGCCAGATGAATGTCCACGACTCTGAGCGCCTTTCGGGCCTGCTCGAGGAAG
CTGGATACGTTGCTGCTCCGGAGGACGCCACTCCGGATCTTGTCGTATTTAATACGTGCGCCGTGCGTGAAAACGCCGAC
ATGCGCCTTTATGGCACTTTGGGCAACCTGCGCAGCGTGAAAGAAAAGAACCCAGGCATGCAAATCGCTGTCGGTGGTTG
TTTGGCTCAAAAAGACAAAGATACCGTGGTGAAAAAAGCACCGTGGGTGGACGTGGTGTTTGGTACCCACAACATTGGTT
CCTTGCCAACCTTGCTTCAGCGCGCGGAGCACAATGCCCAAGCGGAAGTCGAAATTGTCGATTCCCTCGAGCAGTTCCCG
TCAGTACTTCCTGCAAAGCGCGAGTCTGCTTACGCTGGTTGGGTGTCCGTATCAGTCGGATGTAACAACACCTGTACTTT
CTGTATCGTTCCGTCGCTGCGCGGTAAAGAGCAGGACCGTCGACCAGGAGATATCCTCGCAGAGGTACAGGCACTGGTGG
ATCAGGGAGTTACCGAGGTAACTCTACTTGGCCAAAACGTAAATGCTTACGGCGTGAACTTTGTTGATCCTGAGCTAGAG
CGCGATCGCAGTGCATTTTCCAAGCTGCTTCGTGCCTGTGGTGAGATCGAAGGCCTCGAGCGGGTTCGCTTCACCAGCCC
TCACCCTGCGGAATTCACCTCTGATGTCATTGACGCCATGGCAGAGACCCCAAACATCTGCCCGCAGCTGCACATGCCAC
TGCAGTCCGGATCTGACAAGGTGCTCAAAGAGATGCGTCGCTCCTACCGATCCAAGAAGTTCCTCTCCATCTTGGATGAG
GTCCGTGCGAAGATCCCTCACGCCTCTATCACCACCGATATTATTGTCGGATTCCCTGGCGAAACAGAGGAGGATTTCCA
AGCAACCCTCGACGTTGTCAAGAAGGCACGCTTTACTTCTGCTTACACCTTCCAATACAGCCCACGCCCTGGCACCCCTG
CAGCGGAATATGAAAACCAGCTTCCAAAAGAAGTTGTGCAGGAACGCTACGAGCGCCTCATGGTCGTTCAGGAACAAGTT
TGCGAAGAAGAAAACCAAAAGCTCATCGGCACCACCGTCGAATTGCTGGTCCAGGCTGGCGGAGGCCGCAAGAACGATGC
CACCAAGCGCATGAGTGGTCGCGCACGCGATGGACGCCTCGTGCACTTTGCGCCAGAGGGCGACATTGATGGTGAGATCC
GCCCCGGCGATTTTGTCACTGTCACGGTGACTGAGGCCAAGCCTTTCTTCCTCATCGCAGACTCCGGTGTGCAGACCCAC
CGCCGCACCAAAGCTGGTGACAACTCTGCAGTTGGTCAAGTTCCAACCACAGCACCGATCGGTGTGGGCTTGGGACTGCC
ACAAATCGGCGCACCAAAGGTGGCTCCTGCCACAGAATCTGCCTGCTGCTCCATTAACTAA

Upstream 100 bases:

>100_bases
CTAGGAATTCTCAAGGCAAGATCGGAGACGAGCCTTGTTAATTCAGAGTCCTAAATAGGCGTTTATGTCAGCTCGCGCAA
CAGGGATACAATGAGCAACC

Downstream 100 bases:

>100_bases
AAACTGCAGGCTAGAATAGAGGGTCGATAGGAGTCGATGAAAAAGTCGGCGCCAACGAGGAGATGAACAACAACGTGAGT
GATCAAAAGCTAAGTGGTAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MTQQLNHAKVNQHPGQATLPETAEGQVRTYEVKTYGCQMNVHDSERLSGLLEEAGYVAAPEDATPDLVVFNTCAVRENAD
MRLYGTLGNLRSVKEKNPGMQIAVGGCLAQKDKDTVVKKAPWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIVDSLEQFP
SVLPAKRESAYAGWVSVSVGCNNTCTFCIVPSLRGKEQDRRPGDILAEVQALVDQGVTEVTLLGQNVNAYGVNFVDPELE
RDRSAFSKLLRACGEIEGLERVRFTSPHPAEFTSDVIDAMAETPNICPQLHMPLQSGSDKVLKEMRRSYRSKKFLSILDE
VRAKIPHASITTDIIVGFPGETEEDFQATLDVVKKARFTSAYTFQYSPRPGTPAAEYENQLPKEVVQERYERLMVVQEQV
CEEENQKLIGTTVELLVQAGGGRKNDATKRMSGRARDGRLVHFAPEGDIDGEIRPGDFVTVTVTEAKPFFLIADSGVQTH
RRTKAGDNSAVGQVPTTAPIGVGLGLPQIGAPKVAPATESACCSIN

Sequences:

>Translated_526_residues
MTQQLNHAKVNQHPGQATLPETAEGQVRTYEVKTYGCQMNVHDSERLSGLLEEAGYVAAPEDATPDLVVFNTCAVRENAD
MRLYGTLGNLRSVKEKNPGMQIAVGGCLAQKDKDTVVKKAPWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIVDSLEQFP
SVLPAKRESAYAGWVSVSVGCNNTCTFCIVPSLRGKEQDRRPGDILAEVQALVDQGVTEVTLLGQNVNAYGVNFVDPELE
RDRSAFSKLLRACGEIEGLERVRFTSPHPAEFTSDVIDAMAETPNICPQLHMPLQSGSDKVLKEMRRSYRSKKFLSILDE
VRAKIPHASITTDIIVGFPGETEEDFQATLDVVKKARFTSAYTFQYSPRPGTPAAEYENQLPKEVVQERYERLMVVQEQV
CEEENQKLIGTTVELLVQAGGGRKNDATKRMSGRARDGRLVHFAPEGDIDGEIRPGDFVTVTVTEAKPFFLIADSGVQTH
RRTKAGDNSAVGQVPTTAPIGVGLGLPQIGAPKVAPATESACCSIN
>Mature_525_residues
TQQLNHAKVNQHPGQATLPETAEGQVRTYEVKTYGCQMNVHDSERLSGLLEEAGYVAAPEDATPDLVVFNTCAVRENADM
RLYGTLGNLRSVKEKNPGMQIAVGGCLAQKDKDTVVKKAPWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIVDSLEQFPS
VLPAKRESAYAGWVSVSVGCNNTCTFCIVPSLRGKEQDRRPGDILAEVQALVDQGVTEVTLLGQNVNAYGVNFVDPELER
DRSAFSKLLRACGEIEGLERVRFTSPHPAEFTSDVIDAMAETPNICPQLHMPLQSGSDKVLKEMRRSYRSKKFLSILDEV
RAKIPHASITTDIIVGFPGETEEDFQATLDVVKKARFTSAYTFQYSPRPGTPAAEYENQLPKEVVQERYERLMVVQEQVC
EEENQKLIGTTVELLVQAGGGRKNDATKRMSGRARDGRLVHFAPEGDIDGEIRPGDFVTVTVTEAKPFFLIADSGVQTHR
RTKAGDNSAVGQVPTTAPIGVGLGLPQIGAPKVAPATESACCSIN

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=473, Percent_Identity=34.0380549682875, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI28872782, Length=473, Percent_Identity=34.0380549682875, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI93277076, Length=408, Percent_Identity=29.1666666666667, Blast_Score=157, Evalue=3e-38,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=37.5838926174497, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1787057, Length=457, Percent_Identity=31.2910284463895, Blast_Score=204, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17553146, Length=481, Percent_Identity=32.6403326403326, Blast_Score=251, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=219, Percent_Identity=34.2465753424658, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21356207, Length=437, Percent_Identity=35.0114416475973, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI19922432, Length=378, Percent_Identity=30.952380952381, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_CORGB (A4QEV7)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138675.1
- ProteinModelPortal:   A4QEV7
- SMR:   A4QEV7
- STRING:   A4QEV7
- GeneID:   4993240
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1779
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14326
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 57391; Mature: 57260

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQLNHAKVNQHPGQATLPETAEGQVRTYEVKTYGCQMNVHDSERLSGLLEEAGYVAAP
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHCCCEECC
EDATPDLVVFNTCAVRENADMRLYGTLGNLRSVKEKNPGMQIAVGGCLAQKDKDTVVKKA
CCCCCCEEEEEEEEECCCCCEEEEEECHHHHHHHHCCCCCEEEECCHHCCCCCHHHHHCC
PWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIVDSLEQFPSVLPAKRESAYAGWVSVSVG
CCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEECEEEEEEC
CNNTCTFCIVPSLRGKEQDRRPGDILAEVQALVDQGVTEVTLLGQNVNAYGVNFVDPELE
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCHHH
RDRSAFSKLLRACGEIEGLERVRFTSPHPAEFTSDVIDAMAETPNICPQLHMPLQSGSDK
HHHHHHHHHHHHHHCCCCHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
VLKEMRRSYRSKKFLSILDEVRAKIPHASITTDIIVGFPGETEEDFQATLDVVKKARFTS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCE
AYTFQYSPRPGTPAAEYENQLPKEVVQERYERLMVVQEQVCEEENQKLIGTTVELLVQAG
EEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
GGRKNDATKRMSGRARDGRLVHFAPEGDIDGEIRPGDFVTVTVTEAKPFFLIADSGVQTH
CCCCCCHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCHH
RRTKAGDNSAVGQVPTTAPIGVGLGLPQIGAPKVAPATESACCSIN
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQQLNHAKVNQHPGQATLPETAEGQVRTYEVKTYGCQMNVHDSERLSGLLEEAGYVAAP
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHCCCEECC
EDATPDLVVFNTCAVRENADMRLYGTLGNLRSVKEKNPGMQIAVGGCLAQKDKDTVVKKA
CCCCCCEEEEEEEEECCCCCEEEEEECHHHHHHHHCCCCCEEEECCHHCCCCCHHHHHCC
PWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIVDSLEQFPSVLPAKRESAYAGWVSVSVG
CCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEECEEEEEEC
CNNTCTFCIVPSLRGKEQDRRPGDILAEVQALVDQGVTEVTLLGQNVNAYGVNFVDPELE
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCHHH
RDRSAFSKLLRACGEIEGLERVRFTSPHPAEFTSDVIDAMAETPNICPQLHMPLQSGSDK
HHHHHHHHHHHHHHCCCCHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
VLKEMRRSYRSKKFLSILDEVRAKIPHASITTDIIVGFPGETEEDFQATLDVVKKARFTS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCE
AYTFQYSPRPGTPAAEYENQLPKEVVQERYERLMVVQEQVCEEENQKLIGTTVELLVQAG
EEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
GGRKNDATKRMSGRARDGRLVHFAPEGDIDGEIRPGDFVTVTVTEAKPFFLIADSGVQTH
CCCCCCHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCHH
RRTKAGDNSAVGQVPTTAPIGVGLGLPQIGAPKVAPATESACCSIN
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA