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The map label for this gene is miaA

Identifier: 145295849

GI number: 145295849

Start: 1971751

End: 1972656

Strand: Reverse

Name: miaA

Synonym: cgR_1774

Alternate gene names: 145295849

Gene position: 1972656-1971751 (Counterclockwise)

Preceding gene: 145295850

Following gene: 145295848

Centisome position: 59.52

GC content: 55.63

Gene sequence:

>906_bases
GTGGTAACACCGATCGCAGTGGTTGGACCCACTGCATCTGGAAAATCAGCTTTGGGAATTGCTCTAGCCCACAAGCTTGA
CGGTGAAGTAGTCAATGTGGATTCCATGCAGCTGTACAAAGGCATGGACATCGGCACTGCAAAGCTGACTGTCGAAGAAC
GCGAAGGCATTGCGCATCATCAGCTCGATGTCTGGGACGTTACCGAAACTGCGTCAGTGGCGAGGTTTCAATCCGACGCC
GTTGCCGATGTGGAAGATATTATGTCCCGAGGCAAAACCCCTATCTTGGTTGGCGGCTCCATGTTGTACGTCCAATCTTT
GGTCGATGATTGGCAATTCCCACCTACCGACAGCGCTGTTCGCGCACGCTTTGAGGCCCGCCTGGCAGACATCGGTGTCG
AAGCACTACACGCTGAACTTACTCAGCTTGACCCAGAAGCAGCAGCCGTCATCGAAAACAATGATCCCCGACGCACCGTC
CGAGCATTAGAAGTCATTGAACTAACCGGACAGCCCTTCCAGGCAAGCCAACCGCCCAAAGACGCGCCACCTCGCTGGGG
AACTCGAATCATTGGCCTGAAAACCACTCCAGAATGGCTAAATCCACGCATCGAGCAGCGCACCGCCATGATGTTTGAAC
AAGGCTTTGTCGCCGAAGTGGAACACCTTGTGCAGCAAGGACTCATCGCTGACTCCACCGCGGGACGAGCAATCGGCTAC
TCCCAAGTACTGGCAGCCATGGCAGGGGAGATGACCTGGGAAGACGCTTTCGAACGCACGGTCACCGGAACCAGACGCTA
TGTCAGACGCCAACGCAGCTGGTTCAACAGAGACCACCGCGTGTCCTGGGTCGACGCCTCTGGCGATCCCACCGCACAAG
CCTTGGAGATTTTGGGTCTACAATAG

Upstream 100 bases:

>100_bases
ACAGAAGCGTCAACTGCTCAACTTCTCGACAGCGACGACACACACGGCGTGGGACGCTACGTTGCTCGCTGGACTTTTTA
ACTTAAGGGAGATCTAGATC

Downstream 100 bases:

>100_bases
CGAGGGTGAATTTGACCATCCCCTTTGCCAAAGGCCACGCCACCGAAAATGACTTCATCATCATCCCCGATGAGGATGCG
CGCCTAGATTTAACTCCAGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MVTPIAVVGPTASGKSALGIALAHKLDGEVVNVDSMQLYKGMDIGTAKLTVEEREGIAHHQLDVWDVTETASVARFQSDA
VADVEDIMSRGKTPILVGGSMLYVQSLVDDWQFPPTDSAVRARFEARLADIGVEALHAELTQLDPEAAAVIENNDPRRTV
RALEVIELTGQPFQASQPPKDAPPRWGTRIIGLKTTPEWLNPRIEQRTAMMFEQGFVAEVEHLVQQGLIADSTAGRAIGY
SQVLAAMAGEMTWEDAFERTVTGTRRYVRRQRSWFNRDHRVSWVDASGDPTAQALEILGLQ

Sequences:

>Translated_301_residues
MVTPIAVVGPTASGKSALGIALAHKLDGEVVNVDSMQLYKGMDIGTAKLTVEEREGIAHHQLDVWDVTETASVARFQSDA
VADVEDIMSRGKTPILVGGSMLYVQSLVDDWQFPPTDSAVRARFEARLADIGVEALHAELTQLDPEAAAVIENNDPRRTV
RALEVIELTGQPFQASQPPKDAPPRWGTRIIGLKTTPEWLNPRIEQRTAMMFEQGFVAEVEHLVQQGLIADSTAGRAIGY
SQVLAAMAGEMTWEDAFERTVTGTRRYVRRQRSWFNRDHRVSWVDASGDPTAQALEILGLQ
>Mature_301_residues
MVTPIAVVGPTASGKSALGIALAHKLDGEVVNVDSMQLYKGMDIGTAKLTVEEREGIAHHQLDVWDVTETASVARFQSDA
VADVEDIMSRGKTPILVGGSMLYVQSLVDDWQFPPTDSAVRARFEARLADIGVEALHAELTQLDPEAAAVIENNDPRRTV
RALEVIELTGQPFQASQPPKDAPPRWGTRIIGLKTTPEWLNPRIEQRTAMMFEQGFVAEVEHLVQQGLIADSTAGRAIGY
SQVLAAMAGEMTWEDAFERTVTGTRRYVRRQRSWFNRDHRVSWVDASGDPTAQALEILGLQ

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=307, Percent_Identity=28.9902280130293, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1790613, Length=300, Percent_Identity=40, Blast_Score=199, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI25144712, Length=237, Percent_Identity=32.0675105485232, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=32.6388888888889, Blast_Score=149, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24649830, Length=233, Percent_Identity=32.618025751073, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CORGB (A4QEV2)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138670.1
- ProteinModelPortal:   A4QEV2
- SMR:   A4QEV2
- STRING:   A4QEV2
- GeneID:   4991666
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1774
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33085; Mature: 33085

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTPIAVVGPTASGKSALGIALAHKLDGEVVNVDSMQLYKGMDIGTAKLTVEEREGIAHH
CCCCEEEECCCCCCCCHHHHHHHHHCCCCEECCCHHHHHCCCCCCEEEEEEHHHCCCCCC
QLDVWDVTETASVARFQSDAVADVEDIMSRGKTPILVGGSMLYVQSLVDDWQFPPTDSAV
CCEEECCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCCHHH
RARFEARLADIGVEALHAELTQLDPEAAAVIENNDPRRTVRALEVIELTGQPFQASQPPK
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCC
DAPPRWGTRIIGLKTTPEWLNPRIEQRTAMMFEQGFVAEVEHLVQQGLIADSTAGRAIGY
CCCCCCCCEEEEEECCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCH
SQVLAAMAGEMTWEDAFERTVTGTRRYVRRQRSWFNRDHRVSWVDASGDPTAQALEILGL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCC
Q
C
>Mature Secondary Structure
MVTPIAVVGPTASGKSALGIALAHKLDGEVVNVDSMQLYKGMDIGTAKLTVEEREGIAHH
CCCCEEEECCCCCCCCHHHHHHHHHCCCCEECCCHHHHHCCCCCCEEEEEEHHHCCCCCC
QLDVWDVTETASVARFQSDAVADVEDIMSRGKTPILVGGSMLYVQSLVDDWQFPPTDSAV
CCEEECCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCCHHH
RARFEARLADIGVEALHAELTQLDPEAAAVIENNDPRRTVRALEVIELTGQPFQASQPPK
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCC
DAPPRWGTRIIGLKTTPEWLNPRIEQRTAMMFEQGFVAEVEHLVQQGLIADSTAGRAIGY
CCCCCCCCEEEEEECCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCH
SQVLAAMAGEMTWEDAFERTVTGTRRYVRRQRSWFNRDHRVSWVDASGDPTAQALEILGL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA