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The map label for this gene is dapF

Identifier: 145295848

GI number: 145295848

Start: 1970912

End: 1971745

Strand: Reverse

Name: dapF

Synonym: cgR_1773

Alternate gene names: 145295848

Gene position: 1971745-1970912 (Counterclockwise)

Preceding gene: 145295849

Following gene: 145295845

Centisome position: 59.49

GC content: 55.88

Gene sequence:

>834_bases
GTGAATTTGACCATCCCCTTTGCCAAAGGCCACGCCACCGAAAATGACTTCATCATCATCCCCGATGAGGATGCGCGCCT
AGATTTAACTCCAGAAATGGTGGTCAAGCTGTGTGACCGCCGCGCCGGGATCGGTGCTGATGGTATCCTCCGCGTGGTTA
AAGCTGCAGACGTAGAAGGCTCCACGGTCGACCCATCGCTGTGGTTCATGGACTACCGCAACGCCGATGGATCCTTGGCT
GAAATGTGCGGCAATGGTGTGCGCCTGTTCGCGCACTGGCTGTACTCCCGCAGTCTTGTTGATAATACGAGCTTTGATAT
CGGTACCCGCGCCGGTGTCCGCCACGTTGATATTTTGCAGGCAGATCAACATTCTGCGCAGGTCCGCGTTGATATGGGCA
TCCCTGACGTCACGGGATTATCCACCTGCGACATCAACGGCCAAGTATTTGCTGGCCTTGGCGTTGATATGGGTAACCCA
CACCTAGCGTGCGTTGTGCCGGGCTTAAGTGCGTCGGCTCTTGCCGATATGGAACTGCGCGCACCTACGTTTGATCAGGA
ATTCTTCCCCCACGGTGTGAACGTAGAAATCGTCACAGAATTAGAAGATGACGCAGTATCGATGCGCGTGTGGGAACGCG
GAGTGGGCGAAACCCGCTCCTGTGGCACGGGAACCGTTGCTGCAGCGTGTGCTGCTTTAGCTGATGCTGGATTGGGAGAA
GGCACAGTTAAAGTGTGCGTTCCAGGTGGGGAAGTAGAAGTCCAGATCTTTGACGACGGCTCCACACTCACCGGCCCAAG
CGCCATCATCGCACTCGGTGAGGTGCAGATCTAA

Upstream 100 bases:

>100_bases
AACGCAGCTGGTTCAACAGAGACCACCGCGTGTCCTGGGTCGACGCCTCTGGCGATCCCACCGCACAAGCCTTGGAGATT
TTGGGTCTACAATAGCGAGG

Downstream 100 bases:

>100_bases
GATTCGCGATTGTAGTTCGGCCCAAGTTTCTGGGCCGCTTTACGCGCATCCAGCCACGTTTCCCGCAGCTCTAGTGCGCG
CTCGTCCGTTACTTTGAGAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNLTIPFAKGHATENDFIIIPDEDARLDLTPEMVVKLCDRRAGIGADGILRVVKAADVEGSTVDPSLWFMDYRNADGSLA
EMCGNGVRLFAHWLYSRSLVDNTSFDIGTRAGVRHVDILQADQHSAQVRVDMGIPDVTGLSTCDINGQVFAGLGVDMGNP
HLACVVPGLSASALADMELRAPTFDQEFFPHGVNVEIVTELEDDAVSMRVWERGVGETRSCGTGTVAAACAALADAGLGE
GTVKVCVPGGEVEVQIFDDGSTLTGPSAIIALGEVQI

Sequences:

>Translated_277_residues
MNLTIPFAKGHATENDFIIIPDEDARLDLTPEMVVKLCDRRAGIGADGILRVVKAADVEGSTVDPSLWFMDYRNADGSLA
EMCGNGVRLFAHWLYSRSLVDNTSFDIGTRAGVRHVDILQADQHSAQVRVDMGIPDVTGLSTCDINGQVFAGLGVDMGNP
HLACVVPGLSASALADMELRAPTFDQEFFPHGVNVEIVTELEDDAVSMRVWERGVGETRSCGTGTVAAACAALADAGLGE
GTVKVCVPGGEVEVQIFDDGSTLTGPSAIIALGEVQI
>Mature_277_residues
MNLTIPFAKGHATENDFIIIPDEDARLDLTPEMVVKLCDRRAGIGADGILRVVKAADVEGSTVDPSLWFMDYRNADGSLA
EMCGNGVRLFAHWLYSRSLVDNTSFDIGTRAGVRHVDILQADQHSAQVRVDMGIPDVTGLSTCDINGQVFAGLGVDMGNP
HLACVVPGLSASALADMELRAPTFDQEFFPHGVNVEIVTELEDDAVSMRVWERGVGETRSCGTGTVAAACAALADAGLGE
GTVKVCVPGGEVEVQIFDDGSTLTGPSAIIALGEVQI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=289, Percent_Identity=29.4117647058824, Blast_Score=107, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_CORGB (A4QEV1)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138669.1
- ProteinModelPortal:   A4QEV1
- SMR:   A4QEV1
- STRING:   A4QEV1
- GeneID:   4994319
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1773
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 29260; Mature: 29260

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 83-83 ACT_SITE 221-221

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTIPFAKGHATENDFIIIPDEDARLDLTPEMVVKLCDRRAGIGADGILRVVKAADVEG
CCEEEEECCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
STVDPSLWFMDYRNADGSLAEMCGNGVRLFAHWLYSRSLVDNTSFDIGTRAGVRHVDILQ
CCCCCEEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
ADQHSAQVRVDMGIPDVTGLSTCDINGQVFAGLGVDMGNPHLACVVPGLSASALADMELR
CCCCCCEEEEEECCCCCCCCEEECCCCEEEEEECCCCCCCEEEEEECCCCHHHEECEEEC
APTFDQEFFPHGVNVEIVTELEDDAVSMRVWERGVGETRSCGTGTVAAACAALADAGLGE
CCCCCHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCC
GTVKVCVPGGEVEVQIFDDGSTLTGPSAIIALGEVQI
CEEEEEECCCEEEEEEEECCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MNLTIPFAKGHATENDFIIIPDEDARLDLTPEMVVKLCDRRAGIGADGILRVVKAADVEG
CCEEEEECCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
STVDPSLWFMDYRNADGSLAEMCGNGVRLFAHWLYSRSLVDNTSFDIGTRAGVRHVDILQ
CCCCCEEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
ADQHSAQVRVDMGIPDVTGLSTCDINGQVFAGLGVDMGNPHLACVVPGLSASALADMELR
CCCCCCEEEEEECCCCCCCCEEECCCCEEEEEECCCCCCCEEEEEECCCCHHHEECEEEC
APTFDQEFFPHGVNVEIVTELEDDAVSMRVWERGVGETRSCGTGTVAAACAALADAGLGE
CCCCCHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCC
GTVKVCVPGGEVEVQIFDDGSTLTGPSAIIALGEVQI
CEEEEEECCCEEEEEEEECCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA