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The map label for this gene is exoA [H]

Identifier: 145294844

GI number: 145294844

Start: 875012

End: 875926

Strand: Direct

Name: exoA [H]

Synonym: cgR_0791

Alternate gene names: 145294844

Gene position: 875012-875926 (Clockwise)

Preceding gene: 145294843

Following gene: 145294845

Centisome position: 26.4

GC content: 55.41

Gene sequence:

>915_bases
ATGAGTTTTCACATCACATCCGTCAATGTCAACGGCATTAGGGCAGCGGTCAAACAGCGAAGCGAAACAAACCTAGGTTT
CCTTCCGTGGCTTGAAGAAACTCGCCCGGACGTTGTCCTCCTCCAAGAAGTCCGCGCAAGCGAAAAAGACACCGCCACCG
CACTGCAACCCGCCTTAGATAACGGATGGCACTACATTGGTGCCCCAGCAGCTGCCAAGGGACGTGCCGGTGTCGGCATT
TTGTCTAGGCATGAACTTGAAGATGTGAACATCGGTTTTGGATCTTTCCTTGACTCCGGCCGCTACATTGAAGCAACCAT
CAAAGACACCACCCTGGAAGTGCCAGTAACCGTGGCATCTCTTTACCTCCCCTCAGGTTCAGCGGGCACCGACAAGCAGG
ATGAAAAGTACCGCTTCCTCGATGAATTCGAAGGGTTCCTGGACCAGCGCGCTAAAGAACGCTCCCACATGGTCATCGGT
GGCGACTGGAACATCTGCCACCGCCGCGAAGACCTAAAAAACTGGAAAACCAACCAAAAGAAATCCGGTTTCCTTCCCGA
CGAACGCGCATTCATGGATTCAGTCTTTGGCACCTTCCCAGATGAGGCAACCCAGGTTGCAGGGGCCGGCGACTTCTTCG
GTGCCGTGGACTATGAAGGAACGAGGCGTCGAGAAGCAACTACGGACCCTGCGTGGTTCGACGTTGCACGTCGCCTGCAA
CCTGAAGGCGACGGCCCCTACACTTGGTGGACCTACCGCGGAAAAGCCTTCGACACCGGTGCAGGATGGCGCATCGACTA
CCAAGCAGCAACCGCAGCGATGCTCGAACGCGCAGAACGCTCCTGGGTAGACAAAGCCACTGCATACGATTTGCGCTGGT
CAGATCACTCACCACTGAACGTGATCTACTCCTAA

Upstream 100 bases:

>100_bases
TGCCATCGTGGTGTGGTTCCCCATGGTGGCCTTGCGTAAACGCACATAAACGGGGTTGGCGGATGGAGGATTTCCCCAAG
CACCCACTAGTGTGACAAGC

Downstream 100 bases:

>100_bases
AATGCTGCTGACAATTCTATGGGCCATCGGCATCACAGCCGAAGGCATGACAGGCGCGCTGGCCGCAGGCCGACAAAAAA
TGGATCTCTTCGGAGTATCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSFHITSVNVNGIRAAVKQRSETNLGFLPWLEETRPDVVLLQEVRASEKDTATALQPALDNGWHYIGAPAAAKGRAGVGI
LSRHELEDVNIGFGSFLDSGRYIEATIKDTTLEVPVTVASLYLPSGSAGTDKQDEKYRFLDEFEGFLDQRAKERSHMVIG
GDWNICHRREDLKNWKTNQKKSGFLPDERAFMDSVFGTFPDEATQVAGAGDFFGAVDYEGTRRREATTDPAWFDVARRLQ
PEGDGPYTWWTYRGKAFDTGAGWRIDYQAATAAMLERAERSWVDKATAYDLRWSDHSPLNVIYS

Sequences:

>Translated_304_residues
MSFHITSVNVNGIRAAVKQRSETNLGFLPWLEETRPDVVLLQEVRASEKDTATALQPALDNGWHYIGAPAAAKGRAGVGI
LSRHELEDVNIGFGSFLDSGRYIEATIKDTTLEVPVTVASLYLPSGSAGTDKQDEKYRFLDEFEGFLDQRAKERSHMVIG
GDWNICHRREDLKNWKTNQKKSGFLPDERAFMDSVFGTFPDEATQVAGAGDFFGAVDYEGTRRREATTDPAWFDVARRLQ
PEGDGPYTWWTYRGKAFDTGAGWRIDYQAATAAMLERAERSWVDKATAYDLRWSDHSPLNVIYS
>Mature_303_residues
SFHITSVNVNGIRAAVKQRSETNLGFLPWLEETRPDVVLLQEVRASEKDTATALQPALDNGWHYIGAPAAAKGRAGVGIL
SRHELEDVNIGFGSFLDSGRYIEATIKDTTLEVPVTVASLYLPSGSAGTDKQDEKYRFLDEFEGFLDQRAKERSHMVIGG
DWNICHRREDLKNWKTNQKKSGFLPDERAFMDSVFGTFPDEATQVAGAGDFFGAVDYEGTRRREATTDPAWFDVARRLQP
EGDGPYTWWTYRGKAFDTGAGWRIDYQAATAAMLERAERSWVDKATAYDLRWSDHSPLNVIYS

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=302, Percent_Identity=29.1390728476821, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI18375503, Length=302, Percent_Identity=29.1390728476821, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI18375501, Length=302, Percent_Identity=29.1390728476821, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1788046, Length=307, Percent_Identity=25.7328990228013, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 34130; Mature: 33999

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFHITSVNVNGIRAAVKQRSETNLGFLPWLEETRPDVVLLQEVRASEKDTATALQPALD
CEEEEEEECCHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEHHHHHCCCCHHHHHHHHHHH
NGWHYIGAPAAAKGRAGVGILSRHELEDVNIGFGSFLDSGRYIEATIKDTTLEVPVTVAS
CCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCEEECCEEEEE
LYLPSGSAGTDKQDEKYRFLDEFEGFLDQRAKERSHMVIGGDWNICHRREDLKNWKTNQK
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCC
KSGFLPDERAFMDSVFGTFPDEATQVAGAGDFFGAVDYEGTRRREATTDPAWFDVARRLQ
CCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
PEGDGPYTWWTYRGKAFDTGAGWRIDYQAATAAMLERAERSWVDKATAYDLRWSDHSPLN
CCCCCCEEEEEECCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCE
VIYS
EEEC
>Mature Secondary Structure 
SFHITSVNVNGIRAAVKQRSETNLGFLPWLEETRPDVVLLQEVRASEKDTATALQPALD
EEEEEEECCHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEHHHHHCCCCHHHHHHHHHHH
NGWHYIGAPAAAKGRAGVGILSRHELEDVNIGFGSFLDSGRYIEATIKDTTLEVPVTVAS
CCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCEEECCEEEEE
LYLPSGSAGTDKQDEKYRFLDEFEGFLDQRAKERSHMVIGGDWNICHRREDLKNWKTNQK
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCC
KSGFLPDERAFMDSVFGTFPDEATQVAGAGDFFGAVDYEGTRRREATTDPAWFDVARRLQ
CCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
PEGDGPYTWWTYRGKAFDTGAGWRIDYQAATAAMLERAERSWVDKATAYDLRWSDHSPLN
CCCCCCEEEEEECCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCE
VIYS
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]