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The map label for this gene is prpC2 [H]

Identifier: 145294832

GI number: 145294832

Start: 862179

End: 863330

Strand: Direct

Name: prpC2 [H]

Synonym: cgR_0779

Alternate gene names: 145294832

Gene position: 862179-863330 (Clockwise)

Preceding gene: 145294831

Following gene: 145294843

Centisome position: 26.01

GC content: 57.55

Gene sequence:

>1152_bases
ATGTCCAGCACCACAACCACTGATGTTCGCAAAGGACTCTACGGAGTCATCGCGGATTACACGGCCGTTTCCAAAGTCAT
GCCAGAGACCAATTCACTGACCTACCGTGGCTACGCGGTGGAAGATTTGGTGGAAAACTGCAGCTTCGAGGAGGTGTTTT
ACCTCCTGTGGCACGGCGAGCTGCCCACTGCGCAACAACTTGCGGAGTTCAATGAGCGTGGCCGTTCCTACCGCTGTCTG
GATGCCGGTTTGATCTCCCTGATCCACTCTTTGCCTAAAGAAGCCCACCCGATGGATGTTATGCGCACCGCGGTGTCCTA
CATGGGCACCAAGGATTCCGAGTATTTCACCACCGATTCTGAGCACATCCGCAAAGTCGGCCACACCTTGTTGGCGCAGC
TTCCGATGGTGCTAGCCATGGATATTCGTCGCCGCAAAGGCCTCGATATCATCGCCCCTGATTCCACCAAGTCAGTCGCC
GAAAACCTGCTGTCTATGGTGTTTGGCACCGGCCCGGAATCACCTGCATCCAACCCAGCTGACGTCCGCGATTTTGAGAA
ATCACTGATCCTCTACGCCGAGCACTCCTTCAACGCCTCCACCTTCACCGCCCGCGTGATCACCTCCACTAAATCGGATG
TGTACTCCGCAATCACCGGCGCGATCGGTGCTCTCAAGGGCCCATTGCACGGCGGCGCGAACGAGTTTGTCATGCACACC
ATGTTGGAGATCGACGATCCAAATAAGGCCGCCGACTGGATCAACAACGCTTTGGACAACAAGAATGTAGTCATGGGCTT
TGGTCACCGCGTGTACAAGCGTGGCGATTCCCGCGTGCCATCCATGGAGAAGTCCTTCCGGGAACTAGCCGCCCGCCACG
ATGGCGAAAAGTGGGTTGCCATGTATGAAAACATGCGCGACGCCATGGACGCCCGCACCGGCATCAAGCCGAATCTCGAT
TTCCCCGCTGGCCCTGCCTACCACCTGCTCGGCTTCCCGGTCGATTTCTTCACCCCGCTGTTCGTCATCGCGCGCGTCGC
CGGCTGGACCGCACACATCGTGGAGCAGTACGAAAACAACTCGCTCATCCGCCCACTGTCCGAGTACAACGGCGAGGAGC
AGCGCGAGGTCGCGCCCATTGAAAAGCGTTAA

Upstream 100 bases:

>100_bases
TACAACGAGTACAACGCTTTCGACCAGCAAGTATTCACCTATTCCGCTGACAGCTACAAGCCCACCTTCTAACCCGCCTA
TATATAAGGAGTGAATCACC

Downstream 100 bases:

>100_bases
AAGATTTTCGCTTTTCGACGCCCCCTCCCACTCATTTCAGCCATGGTGCAACATTCTCGTCCCCACCATATTCTTCTGAC
TCCCAGGAACAACATGTTTC

Product: citrate synthase

Products: NA

Alternate protein names: Methylcitrate synthase 2; Citrate synthase 2 [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSSTTTTDVRKGLYGVIADYTAVSKVMPETNSLTYRGYAVEDLVENCSFEEVFYLLWHGELPTAQQLAEFNERGRSYRCL
DAGLISLIHSLPKEAHPMDVMRTAVSYMGTKDSEYFTTDSEHIRKVGHTLLAQLPMVLAMDIRRRKGLDIIAPDSTKSVA
ENLLSMVFGTGPESPASNPADVRDFEKSLILYAEHSFNASTFTARVITSTKSDVYSAITGAIGALKGPLHGGANEFVMHT
MLEIDDPNKAADWINNALDNKNVVMGFGHRVYKRGDSRVPSMEKSFRELAARHDGEKWVAMYENMRDAMDARTGIKPNLD
FPAGPAYHLLGFPVDFFTPLFVIARVAGWTAHIVEQYENNSLIRPLSEYNGEEQREVAPIEKR

Sequences:

>Translated_383_residues
MSSTTTTDVRKGLYGVIADYTAVSKVMPETNSLTYRGYAVEDLVENCSFEEVFYLLWHGELPTAQQLAEFNERGRSYRCL
DAGLISLIHSLPKEAHPMDVMRTAVSYMGTKDSEYFTTDSEHIRKVGHTLLAQLPMVLAMDIRRRKGLDIIAPDSTKSVA
ENLLSMVFGTGPESPASNPADVRDFEKSLILYAEHSFNASTFTARVITSTKSDVYSAITGAIGALKGPLHGGANEFVMHT
MLEIDDPNKAADWINNALDNKNVVMGFGHRVYKRGDSRVPSMEKSFRELAARHDGEKWVAMYENMRDAMDARTGIKPNLD
FPAGPAYHLLGFPVDFFTPLFVIARVAGWTAHIVEQYENNSLIRPLSEYNGEEQREVAPIEKR
>Mature_382_residues
SSTTTTDVRKGLYGVIADYTAVSKVMPETNSLTYRGYAVEDLVENCSFEEVFYLLWHGELPTAQQLAEFNERGRSYRCLD
AGLISLIHSLPKEAHPMDVMRTAVSYMGTKDSEYFTTDSEHIRKVGHTLLAQLPMVLAMDIRRRKGLDIIAPDSTKSVAE
NLLSMVFGTGPESPASNPADVRDFEKSLILYAEHSFNASTFTARVITSTKSDVYSAITGAIGALKGPLHGGANEFVMHTM
LEIDDPNKAADWINNALDNKNVVMGFGHRVYKRGDSRVPSMEKSFRELAARHDGEKWVAMYENMRDAMDARTGIKPNLDF
PAGPAYHLLGFPVDFFTPLFVIARVAGWTAHIVEQYENNSLIRPLSEYNGEEQREVAPIEKR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also has a citrate synthase activity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=384, Percent_Identity=24.21875, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786527, Length=372, Percent_Identity=39.7849462365591, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1786939, Length=361, Percent_Identity=31.0249307479224, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17555174, Length=368, Percent_Identity=23.3695652173913, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319850, Length=412, Percent_Identity=24.7572815533981, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324328, Length=368, Percent_Identity=27.1739130434783, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 42758; Mature: 42627

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTTTTDVRKGLYGVIADYTAVSKVMPETNSLTYRGYAVEDLVENCSFEEVFYLLWHGE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHCCCHHHHHHHEECCC
LPTAQQLAEFNERGRSYRCLDAGLISLIHSLPKEAHPMDVMRTAVSYMGTKDSEYFTTDS
CCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEECCH
EHIRKVGHTLLAQLPMVLAMDIRRRKGLDIIAPDSTKSVAENLLSMVFGTGPESPASNPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCC
DVRDFEKSLILYAEHSFNASTFTARVITSTKSDVYSAITGAIGALKGPLHGGANEFVMHT
HHHHHHHHEEEEEECCCCCHHHEEHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MLEIDDPNKAADWINNALDNKNVVMGFGHRVYKRGDSRVPSMEKSFRELAARHDGEKWVA
HHHCCCCCHHHHHHHHHCCCCCEEEECCHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHH
MYENMRDAMDARTGIKPNLDFPAGPAYHLLGFPVDFFTPLFVIARVAGWTAHIVEQYENN
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLIRPLSEYNGEEQREVAPIEKR
CCCCCHHHCCCCHHHHCCCCCCC
>Mature Secondary Structure 
SSTTTTDVRKGLYGVIADYTAVSKVMPETNSLTYRGYAVEDLVENCSFEEVFYLLWHGE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHCCCHHHHHHHEECCC
LPTAQQLAEFNERGRSYRCLDAGLISLIHSLPKEAHPMDVMRTAVSYMGTKDSEYFTTDS
CCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEECCH
EHIRKVGHTLLAQLPMVLAMDIRRRKGLDIIAPDSTKSVAENLLSMVFGTGPESPASNPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCC
DVRDFEKSLILYAEHSFNASTFTARVITSTKSDVYSAITGAIGALKGPLHGGANEFVMHT
HHHHHHHHEEEEEECCCCCHHHEEHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MLEIDDPNKAADWINNALDNKNVVMGFGHRVYKRGDSRVPSMEKSFRELAARHDGEKWVA
HHHCCCCCHHHHHHHHHCCCCCEEEECCHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHH
MYENMRDAMDARTGIKPNLDFPAGPAYHLLGFPVDFFTPLFVIARVAGWTAHIVEQYENN
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLIRPLSEYNGEEQREVAPIEKR
CCCCCHHHCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626 [H]