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The map label for this gene is prpB2 [H]

Identifier: 145294831

GI number: 145294831

Start: 861227

End: 862150

Strand: Direct

Name: prpB2 [H]

Synonym: cgR_0778

Alternate gene names: 145294831

Gene position: 861227-862150 (Clockwise)

Preceding gene: 145294830

Following gene: 145294832

Centisome position: 25.99

GC content: 60.17

Gene sequence:

>924_bases
ATGGCGGGTTTGTTTTCCTCTGCTGTTGCACCAACGGAGCGTCGAAAAGCATTACGCGCAGCACTGGCTGCGCCTGAAAT
TGCCCGCATGCCTGGTGCATTCTCCCCGCTGGCGGCGCGCGCAATCCAGGAAGCCGGATTTGAAGGCGTGTACGTCTCGG
GCGCCGTCGTGGCGGCTGACCTTGCATTGCCGGATATCGGCCTGACCACATTGACCGAAGTAGCGCACCGCTCCCGGCAG
ATCGCACGCGTGACAGACTTGCCCGTGCTGGTCGACGCCGACACCGGATTCGGCGAACCCATGTCCGCAGCGCGCACCGT
CTCCGAACTCGAAGATGCAGGTGTCGCGGGCTGCCACCTGGAAGATCAAGTCAACCCCAAACGCTGTGGGCACCTGGACG
GAAAAGAAGTAGTGGGCACGGACATCATGGTTCGTCGCATCGCCGCAGCTGTCAACGAGCGTCGCGATGAGCAATTCGTC
ATCTGCGCCCGCACCGACGCCGCGGGAGTGGAAGGCATCGACTCCGCGATCGAGCGCGCCAAAGCTTACGCGGATGCCGG
CGCCGACATGATCTTCACCGAAGCGCTGTACAGCCCTGCAGATTTTGAAAAATTCCGCGCGGCCGTCGACATTCCGCTGC
TGGCCAACATGACGGAATTTGGCAAAACCGAACTTCTGCCCGCGCAGCTTCTAGAAGACATCGGCTACAACGCAGTGATC
TACCCAGTGACCCTGCTGCGCATTGCGATGGGACAGGTCGAACAAGCTCTCGGCGACATCGCAAACACCGGAACCCAAAC
CGACTGGGTCGACCGGATGCAACACCGATCCAGGCTGTATGAGCTGCTGCGCTACAACGAGTACAACGCTTTCGACCAGC
AAGTATTCACCTATTCCGCTGACAGCTACAAGCCCACCTTCTAA

Upstream 100 bases:

>100_bases
CACAGCGTACGCACGAGCTTGAGGATCTTTCAGAACTCAACATTGAATTGGATGCCGATATTTTGGCCAAGGCTCCTGTG
ATTCCGGAAGGACTGTTCTG

Downstream 100 bases:

>100_bases
CCCGCCTATATATAAGGAGTGAATCACCATGTCCAGCACCACAACCACTGATGTTCGCAAAGGACTCTACGGAGTCATCG
CGGATTACACGGCCGTTTCC

Product: hypothetical protein

Products: NA

Alternate protein names: 2-methylisocitrate lyase 2 [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MAGLFSSAVAPTERRKALRAALAAPEIARMPGAFSPLAARAIQEAGFEGVYVSGAVVAADLALPDIGLTTLTEVAHRSRQ
IARVTDLPVLVDADTGFGEPMSAARTVSELEDAGVAGCHLEDQVNPKRCGHLDGKEVVGTDIMVRRIAAAVNERRDEQFV
ICARTDAAGVEGIDSAIERAKAYADAGADMIFTEALYSPADFEKFRAAVDIPLLANMTEFGKTELLPAQLLEDIGYNAVI
YPVTLLRIAMGQVEQALGDIANTGTQTDWVDRMQHRSRLYELLRYNEYNAFDQQVFTYSADSYKPTF

Sequences:

>Translated_307_residues
MAGLFSSAVAPTERRKALRAALAAPEIARMPGAFSPLAARAIQEAGFEGVYVSGAVVAADLALPDIGLTTLTEVAHRSRQ
IARVTDLPVLVDADTGFGEPMSAARTVSELEDAGVAGCHLEDQVNPKRCGHLDGKEVVGTDIMVRRIAAAVNERRDEQFV
ICARTDAAGVEGIDSAIERAKAYADAGADMIFTEALYSPADFEKFRAAVDIPLLANMTEFGKTELLPAQLLEDIGYNAVI
YPVTLLRIAMGQVEQALGDIANTGTQTDWVDRMQHRSRLYELLRYNEYNAFDQQVFTYSADSYKPTF
>Mature_306_residues
AGLFSSAVAPTERRKALRAALAAPEIARMPGAFSPLAARAIQEAGFEGVYVSGAVVAADLALPDIGLTTLTEVAHRSRQI
ARVTDLPVLVDADTGFGEPMSAARTVSELEDAGVAGCHLEDQVNPKRCGHLDGKEVVGTDIMVRRIAAAVNERRDEQFVI
CARTDAAGVEGIDSAIERAKAYADAGADMIFTEALYSPADFEKFRAAVDIPLLANMTEFGKTELLPAQLLEDIGYNAVIY
PVTLLRIAMGQVEQALGDIANTGTQTDWVDRMQHRSRLYELLRYNEYNAFDQQVFTYSADSYKPTF

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=286, Percent_Identity=43.7062937062937, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1790445, Length=215, Percent_Identity=29.3023255813954, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 33251; Mature: 33120

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLFSSAVAPTERRKALRAALAAPEIARMPGAFSPLAARAIQEAGFEGVYVSGAVVAAD
CCCCHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCCCEEECCHHHHHH
LALPDIGLTTLTEVAHRSRQIARVTDLPVLVDADTGFGEPMSAARTVSELEDAGVAGCHL
HCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
EDQVNPKRCGHLDGKEVVGTDIMVRRIAAAVNERRDEQFVICARTDAAGVEGIDSAIERA
CCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
KAYADAGADMIFTEALYSPADFEKFRAAVDIPLLANMTEFGKTELLPAQLLEDIGYNAVI
HHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCHHHHCHHHHCCCCCCHHHHHHHCCCCEEH
YPVTLLRIAMGQVEQALGDIANTGTQTDWVDRMQHRSRLYELLRYNEYNAFDQQVFTYSA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEECC
DSYKPTF
CCCCCCC
>Mature Secondary Structure 
AGLFSSAVAPTERRKALRAALAAPEIARMPGAFSPLAARAIQEAGFEGVYVSGAVVAAD
CCCHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCCCEEECCHHHHHH
LALPDIGLTTLTEVAHRSRQIARVTDLPVLVDADTGFGEPMSAARTVSELEDAGVAGCHL
HCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCC
EDQVNPKRCGHLDGKEVVGTDIMVRRIAAAVNERRDEQFVICARTDAAGVEGIDSAIERA
CCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
KAYADAGADMIFTEALYSPADFEKFRAAVDIPLLANMTEFGKTELLPAQLLEDIGYNAVI
HHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCHHHHCHHHHCCCCCCHHHHHHHCCCCEEH
YPVTLLRIAMGQVEQALGDIANTGTQTDWVDRMQHRSRLYELLRYNEYNAFDQQVFTYSA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEECC
DSYKPTF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626 [H]