Click here to switch to the map view.

The map label for this gene is aroE

Identifier: 145294543

GI number: 145294543

Start: 551649

End: 552500

Strand: Direct

Name: aroE

Synonym: cgR_0495

Alternate gene names: 145294543

Gene position: 551649-552500 (Clockwise)

Preceding gene: 145294542

Following gene: 145294548

Centisome position: 16.65

GC content: 61.38

Gene sequence:

>852_bases
ATGAACGACAGTATTCTCCTCGGCCTAATCGGCCAGGGCCTCGACCTATCGCGCACCCCCGCAATGCACGAGGCGGAAGG
CCTCGCGCAGGGACGTGCGACCGTGTACAGGCGCATCGACACGCTTGGGTCGCGTGCTTCCGGGCAAGATTTAAAGACGC
TTCTCGACGCCGCCCTCTACCTTGGCTTCAACGGCCTGAACATCACTCACCCGTACAAACAAGCAGTATTACCCCTGCTT
GACGAAGTCTCCGAACAAGCCACCCAACTCGGCGCAGTGAATACTGTCGTTATCGACGCCAACGGCCACACCACCGGCCA
CAACACCGACGTCTCCGGATTTGGCCGCGGAATGGAAGAAGGCCTCCCCAACGCCAAGCTCGATTCCGTCGTGCAGGTCG
GCGCCGGCGGCGTAGGAAACGCAGTGGCATACGCCCTGGTCACCCATGGTGTGCAGAAACTTCAGGTCGCTGACCTCGAC
ACTTCCCGCGCACAGGCATTAGCGGATGTCATCAACAATGCAGTCGGCCGCGAAGCTGTCGTGGGAGTAGACGCCCGCGG
CATCGAAGACGTCATCGCAGCCGCCGACGGAGTAGTCAACGCAACCCCCATGGGAATGCCCGCACACCCCGGCACCGCCT
TTGATGTCAGCTGCCTCACCAAGGATCACTGGGTTGGCGACGTCGTGTACATGCCCATCGAAACTGAACTTCTCAAGGCC
GCCCGTGCCCTCGGCTGCGAAACCCTCGACGGAACCCGCATGGCAATCCACCAAGCCGTCGATGCCTTCCGCCTGTTCAC
CGGCCTCGAACCCGACGTCTCCCGCATGCGGGAAACTTTCCTATCCCTCTAA

Upstream 100 bases:

>100_bases
CCCTCGCCGCGGTCTCCGTTATCGCTGGCGCTGGCATCCAGGGTTACCGTTTCGCGGTCGATATCCTGGCAAATCTCAAA
AAGTAGAAAGCCCAAAAAAT

Downstream 100 bases:

>100_bases
AAGAGTCAGTAAAACCTCGACGCTTCGACCGTTTCCTTCGCTTCCTTCAACCCTGCCCCGGTAAGTTCACGGTGACGTTT
GATCGCCGCGATCTTCTTAC

Product: quinate/shikimate dehydrogenase

Products: NA

Alternate protein names: NAD(+)-dependent quinate dehydrogenase; QDH

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MNDSILLGLIGQGLDLSRTPAMHEAEGLAQGRATVYRRIDTLGSRASGQDLKTLLDAALYLGFNGLNITHPYKQAVLPLL
DEVSEQATQLGAVNTVVIDANGHTTGHNTDVSGFGRGMEEGLPNAKLDSVVQVGAGGVGNAVAYALVTHGVQKLQVADLD
TSRAQALADVINNAVGREAVVGVDARGIEDVIAAADGVVNATPMGMPAHPGTAFDVSCLTKDHWVGDVVYMPIETELLKA
ARALGCETLDGTRMAIHQAVDAFRLFTGLEPDVSRMRETFLSL

Sequences:

>Translated_283_residues
MNDSILLGLIGQGLDLSRTPAMHEAEGLAQGRATVYRRIDTLGSRASGQDLKTLLDAALYLGFNGLNITHPYKQAVLPLL
DEVSEQATQLGAVNTVVIDANGHTTGHNTDVSGFGRGMEEGLPNAKLDSVVQVGAGGVGNAVAYALVTHGVQKLQVADLD
TSRAQALADVINNAVGREAVVGVDARGIEDVIAAADGVVNATPMGMPAHPGTAFDVSCLTKDHWVGDVVYMPIETELLKA
ARALGCETLDGTRMAIHQAVDAFRLFTGLEPDVSRMRETFLSL
>Mature_283_residues
MNDSILLGLIGQGLDLSRTPAMHEAEGLAQGRATVYRRIDTLGSRASGQDLKTLLDAALYLGFNGLNITHPYKQAVLPLL
DEVSEQATQLGAVNTVVIDANGHTTGHNTDVSGFGRGMEEGLPNAKLDSVVQVGAGGVGNAVAYALVTHGVQKLQVADLD
TSRAQALADVINNAVGREAVVGVDARGIEDVIAAADGVVNATPMGMPAHPGTAFDVSCLTKDHWVGDVVYMPIETELLKA
ARALGCETLDGTRMAIHQAVDAFRLFTGLEPDVSRMRETFLSL

Specific function: Catalyzes the NAD(+)-dependent oxidation of both quinate and shikimate to 3-dehydroquinate and 3-dehydroshikimate, respectively. Seems to play a key role in the quinate degradation pathway

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787983, Length=282, Percent_Identity=27.3049645390071, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789675, Length=270, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320332, Length=293, Percent_Identity=25.5972696245734, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_CORGB (A4QB65)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001137364.1
- ProteinModelPortal:   A4QB65
- SMR:   A4QB65
- STRING:   A4QB65
- GeneID:   4994338
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_0495
- eggNOG:   COG0169
- HOGENOM:   HBG553408
- OMA:   GFNGLNI
- ProtClustDB:   PRK14027
- GO:   GO:0005737
- GO:   GO:0005488
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.24

Molecular weight: Translated: 29710; Mature: 29710

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00962 RIBOSOMAL_S2_1

Important sites: ACT_SITE 73-73 BINDING 110-110 BINDING 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSILLGLIGQGLDLSRTPAMHEAEGLAQGRATVYRRIDTLGSRASGQDLKTLLDAALY
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LGFNGLNITHPYKQAVLPLLDEVSEQATQLGAVNTVVIDANGHTTGHNTDVSGFGRGMEE
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCCHHH
GLPNAKLDSVVQVGAGGVGNAVAYALVTHGVQKLQVADLDTSRAQALADVINNAVGREAV
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHCHHHEEEECCCHHHHHHHHHHHHHHCCCHHE
VGVDARGIEDVIAAADGVVNATPMGMPAHPGTAFDVSCLTKDHWVGDVVYMPIETELLKA
EECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHH
ARALGCETLDGTRMAIHQAVDAFRLFTGLEPDVSRMRETFLSL
HHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNDSILLGLIGQGLDLSRTPAMHEAEGLAQGRATVYRRIDTLGSRASGQDLKTLLDAALY
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LGFNGLNITHPYKQAVLPLLDEVSEQATQLGAVNTVVIDANGHTTGHNTDVSGFGRGMEE
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCCHHH
GLPNAKLDSVVQVGAGGVGNAVAYALVTHGVQKLQVADLDTSRAQALADVINNAVGREAV
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHCHHHEEEECCCHHHHHHHHHHHHHHCCCHHE
VGVDARGIEDVIAAADGVVNATPMGMPAHPGTAFDVSCLTKDHWVGDVVYMPIETELLKA
EECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHH
ARALGCETLDGTRMAIHQAVDAFRLFTGLEPDVSRMRETFLSL
HHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA