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The map label for this gene is yhcR [H]

Identifier: 145294457

GI number: 145294457

Start: 448329

End: 450395

Strand: Direct

Name: yhcR [H]

Synonym: cgR_0412

Alternate gene names: 145294457

Gene position: 448329-450395 (Clockwise)

Preceding gene: 145294456

Following gene: 145294459

Centisome position: 13.53

GC content: 56.36

Gene sequence:

>2067_bases
ATGAAGCGGCTTTCCCGCGCAGCACTCGCAGTGGTCGCCACCACCGCGGTTAGCTTCGGCGCACTCGCAGTCCCAGCTTT
CGCAGATGAAGCAAACAATGTTGAACTCAACATCCTCGGTGTTACCGACTTCCACGGTCACATCGAGCAGAAGGCTGTTA
AAGACGATGACGGTGTAATCACCGGTTACTCAGAAATGGGCGCTAGCGGCGTTGCCTGCTACGTCGACGCTGAGCGTGAG
GCCAACCCAAACACCAGCTTCATCACCGTTGGTGACAACATTGGTGGATCCCCATTCGTATCCTCCATCCTGAAAGACGA
GCCAACCCTTCAAGCTCTCAACGCCATCGGTGTTGACGCATCCGCACTGGGTAACCACGAATTCGACCAGGGCTACGCAG
ACCTGACCGACCGCGTCTCCCTCGACGGCACCGGCAGCGCACAGTTCCCATACCTCGGCGCAAACGTTGAAGGTGGCAAC
CCAGCACCTGCAAAATCTGAAATCATCGAGATGGACGGCGTCAAGATCGCTTACGTCGGTGCCGTAACCGAAGAGACCGC
AACCTTGGTCTCCCCGGCAGGCATCGAAGGCATCACCTTCACCGGCGACATTGACGCCATTAACGCAGAAGCAGATCGCG
TCATTGAGGCAGGCGAAGCAGACGTAGTCATCGCACTGCTCCACGCTGAAGCCGCTCCAACCGATCCATTCTCCAACAAC
GTTGACGTGGTATTCTCCGGCCACACCCACTTTGATTACGTTGCTGAAGGCGAAGCACGTGGCGACAAGCAGCCACTCGT
TGTCATCCAGGGCCACGAATACGGCAAGGTCATCTCCGACGCGGAGATCTCCTACGACCGCGAAGCAGGCAAGATCACCA
ACATTGAGGCGAAGAATATCTCTGCTACTGACGTTGTGGAAAACTGTGAGACTCCAAACGCAGAAGTTGACACAATCGTT
GCGGCTGCAGTTGATGCTGCTGAAGAAGCAGGTAATGAAGTTGTTGCAACCATTGACAACGGCTTCTTCCGTGGTGCAAA
CGAAGAGGGTGCAACCGGTTCCAACCGTGGTGTTGAGTCTTCCCTGAGCAACCTCATCGCAGAAGCTGGTCTGTGGGCAG
TCAACGACGCGACCATCCTGAACGCTGATATCGGCATCATGAACGCAGGCGGCGTGCGTGCGGACCTCGAAGCAGGCGAA
GTTACCTTCGCAGATGCATACGCAACCCAGAACTTCTCCAACACCTATGGCGCACGTGACCTGACCGGTGCGCAGTTCAA
AGAAGCGCTGGAACAGCAGTGGAAGCTGACCGGCGACCGCCCACGTCTGGCATTGGGACTGTCCAGCAACGTCCAGTACT
CCTACGACGAGACCCGCGAATACGGCGACCGCATCACCCACATCACCTTCAACGGTGAGCCAATGGATATGAAGGAGACC
TACCGCGTCACAGGATCATCCTTCCTGCTGGCAGGTGGCGACTCCTTCACTGCATTCGCTGAAGGCGGCCCAATCGCTGA
AACCGGCATGGTTGACATTGACCTGTTCAACAACTACATCGCAGCTCACCCAGATGCACCAATTCGTGCAAATCAGAGCT
CTGTAGGCATCGCCCTTTCCGGCCCAGCAGTTGCAGAAGACGGAACTTTGGTCCCTGGTGAAGAGCTGACCGTTGAGCTT
TCCTCCCTGTCCTACACCGGACCTGAAGAAAAGCCTTCCACTGTTGAAGTGACCGTCGGTTCTGAGAAGAAGACTGCGGA
CGTTGATAACACCATCGTTCCTCAGCTGGATCAGACCGGTACCGCATCTGTCACCCTGACTGTTCCTGCGGGAGCAACTT
CTGTTGAGATCGCTACCGACAATGGCACCACGTTCACCCTTCCAGTATCCGCAACTGGTATGGACGACTCTGAGCAGCTG
TCCTCCGGATCCTCCGACGCGGGTTCCCTGGTGGCAGTTCTCGGTGTTCTTGGAGCACTTGGTGGCCTGGTCGCATTCTT
CCTGAACTCTGCACAGGGCGCACCATTCCTGGCTCAGATTCAGGCTGTGCTTGCGCAGTTCATGTAA

Upstream 100 bases:

>100_bases
AATATCATCTTTCGCTAATGGAAAGCCCCAGCTCACCGAATTCTCCATTCGTTTTAATTGCTTCGTTAACTAAAACCAGG
CTCTTTTCCAGGAGAATTTA

Downstream 100 bases:

>100_bases
GAACTTGCAGTAAGTAAATCGGGCCCTTCCCAAGAGATTCTTTCTTGGGAAGGACCTGATTTTTGCTTGGAAAGCTAGAT
TTAAAAAATAGCCCCTGATT

Product: hypothetical protein

Products: Phosphate; Uridine [C]

Alternate protein names: NA

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MKRLSRAALAVVATTAVSFGALAVPAFADEANNVELNILGVTDFHGHIEQKAVKDDDGVITGYSEMGASGVACYVDAERE
ANPNTSFITVGDNIGGSPFVSSILKDEPTLQALNAIGVDASALGNHEFDQGYADLTDRVSLDGTGSAQFPYLGANVEGGN
PAPAKSEIIEMDGVKIAYVGAVTEETATLVSPAGIEGITFTGDIDAINAEADRVIEAGEADVVIALLHAEAAPTDPFSNN
VDVVFSGHTHFDYVAEGEARGDKQPLVVIQGHEYGKVISDAEISYDREAGKITNIEAKNISATDVVENCETPNAEVDTIV
AAAVDAAEEAGNEVVATIDNGFFRGANEEGATGSNRGVESSLSNLIAEAGLWAVNDATILNADIGIMNAGGVRADLEAGE
VTFADAYATQNFSNTYGARDLTGAQFKEALEQQWKLTGDRPRLALGLSSNVQYSYDETREYGDRITHITFNGEPMDMKET
YRVTGSSFLLAGGDSFTAFAEGGPIAETGMVDIDLFNNYIAAHPDAPIRANQSSVGIALSGPAVAEDGTLVPGEELTVEL
SSLSYTGPEEKPSTVEVTVGSEKKTADVDNTIVPQLDQTGTASVTLTVPAGATSVEIATDNGTTFTLPVSATGMDDSEQL
SSGSSDAGSLVAVLGVLGALGGLVAFFLNSAQGAPFLAQIQAVLAQFM

Sequences:

>Translated_688_residues
MKRLSRAALAVVATTAVSFGALAVPAFADEANNVELNILGVTDFHGHIEQKAVKDDDGVITGYSEMGASGVACYVDAERE
ANPNTSFITVGDNIGGSPFVSSILKDEPTLQALNAIGVDASALGNHEFDQGYADLTDRVSLDGTGSAQFPYLGANVEGGN
PAPAKSEIIEMDGVKIAYVGAVTEETATLVSPAGIEGITFTGDIDAINAEADRVIEAGEADVVIALLHAEAAPTDPFSNN
VDVVFSGHTHFDYVAEGEARGDKQPLVVIQGHEYGKVISDAEISYDREAGKITNIEAKNISATDVVENCETPNAEVDTIV
AAAVDAAEEAGNEVVATIDNGFFRGANEEGATGSNRGVESSLSNLIAEAGLWAVNDATILNADIGIMNAGGVRADLEAGE
VTFADAYATQNFSNTYGARDLTGAQFKEALEQQWKLTGDRPRLALGLSSNVQYSYDETREYGDRITHITFNGEPMDMKET
YRVTGSSFLLAGGDSFTAFAEGGPIAETGMVDIDLFNNYIAAHPDAPIRANQSSVGIALSGPAVAEDGTLVPGEELTVEL
SSLSYTGPEEKPSTVEVTVGSEKKTADVDNTIVPQLDQTGTASVTLTVPAGATSVEIATDNGTTFTLPVSATGMDDSEQL
SSGSSDAGSLVAVLGVLGALGGLVAFFLNSAQGAPFLAQIQAVLAQFM
>Mature_688_residues
MKRLSRAALAVVATTAVSFGALAVPAFADEANNVELNILGVTDFHGHIEQKAVKDDDGVITGYSEMGASGVACYVDAERE
ANPNTSFITVGDNIGGSPFVSSILKDEPTLQALNAIGVDASALGNHEFDQGYADLTDRVSLDGTGSAQFPYLGANVEGGN
PAPAKSEIIEMDGVKIAYVGAVTEETATLVSPAGIEGITFTGDIDAINAEADRVIEAGEADVVIALLHAEAAPTDPFSNN
VDVVFSGHTHFDYVAEGEARGDKQPLVVIQGHEYGKVISDAEISYDREAGKITNIEAKNISATDVVENCETPNAEVDTIV
AAAVDAAEEAGNEVVATIDNGFFRGANEEGATGSNRGVESSLSNLIAEAGLWAVNDATILNADIGIMNAGGVRADLEAGE
VTFADAYATQNFSNTYGARDLTGAQFKEALEQQWKLTGDRPRLALGLSSNVQYSYDETREYGDRITHITFNGEPMDMKET
YRVTGSSFLLAGGDSFTAFAEGGPIAETGMVDIDLFNNYIAAHPDAPIRANQSSVGIALSGPAVAEDGTLVPGEELTVEL
SSLSYTGPEEKPSTVEVTVGSEKKTADVDNTIVPQLDQTGTASVTLTVPAGATSVEIATDNGTTFTLPVSATGMDDSEQL
SSGSSDAGSLVAVLGVLGALGGLVAFFLNSAQGAPFLAQIQAVLAQFM

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=555, Percent_Identity=24.6846846846847, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1786687, Length=571, Percent_Identity=24.1681260945709, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1790658, Length=564, Percent_Identity=23.936170212766, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24641187, Length=486, Percent_Identity=25.9259259259259, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28573524, Length=571, Percent_Identity=23.9929947460595, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI221329836, Length=529, Percent_Identity=22.8733459357278, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922446, Length=544, Percent_Identity=22.9779411764706, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654424, Length=544, Percent_Identity=22.9779411764706, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922444, Length=542, Percent_Identity=23.6162361623616, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161076508, Length=457, Percent_Identity=24.0700218818381, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921980, Length=457, Percent_Identity=24.0700218818381, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652512, Length=457, Percent_Identity=24.0700218818381, Blast_Score=85, Evalue=2e-16,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.6.1.45; 3.1.3.5 [C]

Molecular weight: Translated: 71314; Mature: 71314

Theoretical pI: Translated: 3.85; Mature: 3.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLSRAALAVVATTAVSFGALAVPAFADEANNVELNILGVTDFHGHIEQKAVKDDDGVI
CCCHHHHHHHHHHHHHHHCCCEECCHHCCCCCCEEEEEEEEECCCCHHHHHHCCCCCCEE
TGYSEMGASGVACYVDAEREANPNTSFITVGDNIGGSPFVSSILKDEPTLQALNAIGVDA
ECHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHCCCCH
SALGNHEFDQGYADLTDRVSLDGTGSAQFPYLGANVEGGNPAPAKSEIIEMDGVKIAYVG
HHCCCCCCCCCHHHHHHCEEECCCCCCCCCEEECCCCCCCCCCCHHHEEEECCEEEEEEE
AVTEETATLVSPAGIEGITFTGDIDAINAEADRVIEAGEADVVIALLHAEAAPTDPFSNN
EECCCHHHEECCCCCCCEEEECCCCEECCCHHHHEECCCCCEEEEEEECCCCCCCCCCCC
VDVVFSGHTHFDYVAEGEARGDKQPLVVIQGHEYGKVISDAEISYDREAGKITNIEAKNI
EEEEEECCCCEEEEECCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCCCCEEEEECCCC
SATDVVENCETPNAEVDTIVAAAVDAAEEAGNEVVATIDNGFFRGANEEGATGSNRGVES
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCCCCCHHH
SLSNLIAEAGLWAVNDATILNADIGIMNAGGVRADLEAGEVTFADAYATQNFSNTYGARD
HHHHHHHHCCCEEECCCEEEECCCEEEECCCCEEECCCCCEEEEHHHHHCCCCCCCCCCC
LTGAQFKEALEQQWKLTGDRPRLALGLSSNVQYSYDETREYGDRITHITFNGEPMDMKET
CCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEECHHHHHHHCCEEEEEEECCCCCCHHHH
YRVTGSSFLLAGGDSFTAFAEGGPIAETGMVDIDLFNNYIAAHPDAPIRANQSSVGIALS
EEECCCEEEEECCCCEEEEECCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCEEEEEC
GPAVAEDGTLVPGEELTVELSSLSYTGPEEKPSTVEVTVGSEKKTADVDNTIVPQLDQTG
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
TASVTLTVPAGATSVEIATDNGTTFTLPVSATGMDDSEQLSSGSSDAGSLVAVLGVLGAL
CEEEEEEECCCCCEEEEEECCCCEEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHH
GGLVAFFLNSAQGAPFLAQIQAVLAQFM
HHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKRLSRAALAVVATTAVSFGALAVPAFADEANNVELNILGVTDFHGHIEQKAVKDDDGVI
CCCHHHHHHHHHHHHHHHCCCEECCHHCCCCCCEEEEEEEEECCCCHHHHHHCCCCCCEE
TGYSEMGASGVACYVDAEREANPNTSFITVGDNIGGSPFVSSILKDEPTLQALNAIGVDA
ECHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHCCCCH
SALGNHEFDQGYADLTDRVSLDGTGSAQFPYLGANVEGGNPAPAKSEIIEMDGVKIAYVG
HHCCCCCCCCCHHHHHHCEEECCCCCCCCCEEECCCCCCCCCCCHHHEEEECCEEEEEEE
AVTEETATLVSPAGIEGITFTGDIDAINAEADRVIEAGEADVVIALLHAEAAPTDPFSNN
EECCCHHHEECCCCCCCEEEECCCCEECCCHHHHEECCCCCEEEEEEECCCCCCCCCCCC
VDVVFSGHTHFDYVAEGEARGDKQPLVVIQGHEYGKVISDAEISYDREAGKITNIEAKNI
EEEEEECCCCEEEEECCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCCCCEEEEECCCC
SATDVVENCETPNAEVDTIVAAAVDAAEEAGNEVVATIDNGFFRGANEEGATGSNRGVES
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCCCCCHHH
SLSNLIAEAGLWAVNDATILNADIGIMNAGGVRADLEAGEVTFADAYATQNFSNTYGARD
HHHHHHHHCCCEEECCCEEEECCCEEEECCCCEEECCCCCEEEEHHHHHCCCCCCCCCCC
LTGAQFKEALEQQWKLTGDRPRLALGLSSNVQYSYDETREYGDRITHITFNGEPMDMKET
CCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEECHHHHHHHCCEEEEEEECCCCCCHHHH
YRVTGSSFLLAGGDSFTAFAEGGPIAETGMVDIDLFNNYIAAHPDAPIRANQSSVGIALS
EEECCCEEEEECCCCEEEEECCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCEEEEEC
GPAVAEDGTLVPGEELTVELSSLSYTGPEEKPSTVEVTVGSEKKTADVDNTIVPQLDQTG
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
TASVTLTVPAGATSVEIATDNGTTFTLPVSATGMDDSEQLSSGSSDAGSLVAVLGVLGAL
CEEEEEEECCCCCEEEEEECCCCEEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHH
GGLVAFFLNSAQGAPFLAQIQAVLAQFM
HHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; UMP [C]

Specific reaction: H2O + UMP --> Phosphate + Uridine [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Inhibitor from Salmonella typhimurium cytoplasm [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]