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The map label for this gene is mcrC [H]

Identifier: 145294213

GI number: 145294213

Start: 186192

End: 186713

Strand: Direct

Name: mcrC [H]

Synonym: cgR_0170

Alternate gene names: 145294213

Gene position: 186192-186713 (Clockwise)

Preceding gene: 145294212

Following gene: 145294216

Centisome position: 5.62

GC content: 50.57

Gene sequence:

>522_bases
ATGAAGCTTGCTTCGTGGCTTTCAGATGATGTGATGAGCAGCCTGTATGAACGATTTTTGCGCGAGTATTATCGCTTCCA
TCACCCTGATCTCTCGCCTAAGGGGTCTGTTCTGTCATGGCACTATGATCACGGCAGTGCTTTTGGGGCGGAGCAGTTGC
CAGTAATGCGCACAGACCTCACGCTCCGAAAAGCGGGGCGCACACTTATTATCGACGCGAAATATTACAGTCAGTCGATG
CAGAGTGGGATGTCGGGAAAAGAGACAGTGCATTCAGCGCATCTTTACCAAATATTGGCTTACGCCAAGAATGCAGATGT
CGACCAGAATGGCTCGGTCAGTGGCCTGCTTCTCTACGCACGCACAGAGGCAGTAAAGCAGCCTGATCTGGATGTAGTTA
TCCAGGGCAACAGGATTGGTGCTCGAACGCTGGATCTTAATTGTCCGTGGAGTGAAATTAGTGCTGAGCTGGAAAGTATC
GTGAGATGGCTTGAGGACGGAGCTGAATATTCGCGGTCATGA

Upstream 100 bases:

>100_bases
CACGCTTACTTATCATCGCGCTAATGCTTCATATCGGCTTTTGTTGGGTGTCTGTGAGCTAGTTGTGTGTGGGCTTCTTC
CTGCCCAGGGTGCTGGGAAC

Downstream 100 bases:

>100_bases
GAGCCAGTGATGGTGTGCATCAGAGCCACTTTCTCTTAAGAGCGGTTGGTCTCCCCGGGAGCCATCCGTGGTGTCCAGGA
GAGCCGCTGCGGTTCCTTCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MKLASWLSDDVMSSLYERFLREYYRFHHPDLSPKGSVLSWHYDHGSAFGAEQLPVMRTDLTLRKAGRTLIIDAKYYSQSM
QSGMSGKETVHSAHLYQILAYAKNADVDQNGSVSGLLLYARTEAVKQPDLDVVIQGNRIGARTLDLNCPWSEISAELESI
VRWLEDGAEYSRS

Sequences:

>Translated_173_residues
MKLASWLSDDVMSSLYERFLREYYRFHHPDLSPKGSVLSWHYDHGSAFGAEQLPVMRTDLTLRKAGRTLIIDAKYYSQSM
QSGMSGKETVHSAHLYQILAYAKNADVDQNGSVSGLLLYARTEAVKQPDLDVVIQGNRIGARTLDLNCPWSEISAELESI
VRWLEDGAEYSRS
>Mature_173_residues
MKLASWLSDDVMSSLYERFLREYYRFHHPDLSPKGSVLSWHYDHGSAFGAEQLPVMRTDLTLRKAGRTLIIDAKYYSQSM
QSGMSGKETVHSAHLYQILAYAKNADVDQNGSVSGLLLYARTEAVKQPDLDVVIQGNRIGARTLDLNCPWSEISAELESI
VRWLEDGAEYSRS

Specific function: Modifies the specificity of mcrB restriction by expanding the range of modified sequences restricted. Does not bind to DNA [H]

COG id: COG4268

COG function: function code V; McrBC 5-methylcytosine restriction system component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790804, Length=150, Percent_Identity=36, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014407
- InterPro:   IPR019292 [H]

Pfam domain/function: PF10117 McrBC [H]

EC number: NA

Molecular weight: Translated: 19562; Mature: 19562

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLASWLSDDVMSSLYERFLREYYRFHHPDLSPKGSVLSWHYDHGSAFGAEQLPVMRTDL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHCH
TLRKAGRTLIIDAKYYSQSMQSGMSGKETVHSAHLYQILAYAKNADVDQNGSVSGLLLYA
HHHHCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
RTEAVKQPDLDVVIQGNRIGARTLDLNCPWSEISAELESIVRWLEDGAEYSRS
EHHHHCCCCCEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLASWLSDDVMSSLYERFLREYYRFHHPDLSPKGSVLSWHYDHGSAFGAEQLPVMRTDL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHCH
TLRKAGRTLIIDAKYYSQSMQSGMSGKETVHSAHLYQILAYAKNADVDQNGSVSGLLLYA
HHHHCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
RTEAVKQPDLDVVIQGNRIGARTLDLNCPWSEISAELESIVRWLEDGAEYSRS
EHHHHCCCCCEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 2050643 [H]