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The map label for this gene is mcrB [H]

Identifier: 145294212

GI number: 145294212

Start: 184222

End: 185643

Strand: Direct

Name: mcrB [H]

Synonym: cgR_0169

Alternate gene names: 145294212

Gene position: 184222-185643 (Clockwise)

Preceding gene: 145294211

Following gene: 145294213

Centisome position: 5.56

GC content: 48.8

Gene sequence:

>1422_bases
GTGGAACGCTGGAATAATAAACCTCAGCCAGAAGCATCTGAAGATAGTGAAGATCTTGAGCTTGCTGAACCACGTCCGCG
ACATTACTGGCTGTATTCTCCGGGGCCACAAGCCTCAGAGTGGGATGAATTTTCCTCTGAGTCGATTATGGCGATTGGTT
GGGATGATCTGGACGATCTCTCGACCTATCCCAGTCGAGAAGCGATTCGGCAGGCCTTAAATGTCGATGGGGCCGGTGGG
TCGCAGACGAATGCGACTCTAGCGCTCTGGCAATTCCAGAATGATATGACCGTGGGAGATATTGTTTATGCCAAAGAAGG
TAGACAGAAAATAGTTGGCCGCGGGGAGATCACGTCTAATGCTCGTTTTGAACCGGAACGTGAATCATATCGCCATGTCA
GGTCAGTAAAATGGACTCATATTGGAAAGTGGGACCATCCTGGCGATGCGGTAACTAAAACGCTCACCGACATCACCAAA
GATCATGACTATGTAGAAAAGCTTGAAGCACTCATCACTGATGAAGTGGATACAGAACAGCTGGTGTCACCTTCCACGGC
CCCGATTTATGACAAAGCTGCTTTTCTGAGTGAAGTCTATCTTTCCGAGACTCGGTATGTCCGGCTGCGATCCTTGCTGG
GCCGCAAGAAAAACGTCATCCTCGCAGGTCCTCCCGGAGTAGGTAAAACCTACGCCGCTAAACGTCTCGCCTACTCCATT
ATGGGAACAAAAGATCCCAGTCGAGTGCAGATGATCCAATTCCATCAGAGCTACTCTTATGAAGATTTCATGATGGGATA
CCGGCCTACTGAAAATGGGGGGTTCATTATCTCCGAAGGCCCCTTCTACCGATTTTGTGAAAAAGCTCGGGCAGATGATG
CGGACCGGCCCTACTTTTTCATCATTGACGAAATCAACCGCGGCAATATTTCCAAGATTTTTGGTGAGCTACTCATGCTC
ATTGAGTCAGATAAGCGCGGCCAGGAACTACGACTTCTGTATAAAAATGAGACTTTTTCCATTCCTGCCAATGTCCATAT
TATCGGCATGATGAATACGGCTGATCGTAGCCTCGCGGTGCTCGATTATGCATTGCGACGCCGCTTCGGATTCTTCGAGC
TCGCCCCTGCCTACGAATCTGCTGGATTTGCTCAGTGGATTCAAAGTGCTGATAACCCAATGCTTAATCGTCTTGTATCG
GTGATTCTTGACCTTAATAGGGAAATTATTGACGATCCCACCCTTGGGCAAGGTTTTGCGATCGGGCACAGTTTCCTCTC
TCAACCTGTAGGCAACGCTGATGAGGCCTGGCTTTATTCCGTAGTCGAAGACGAAATTATCCCTCTACTCAACGAATACT
GGTTTGATGAACCTGTTAAGGCTGAGCAATGGGCAGATAAGCTCCGGGCAGTGGTCGCATGA

Upstream 100 bases:

>100_bases
AGGCAGTACCCCAGCTGCAATCGATAGTGATCTACTTGCTATCAGATGAGTTTTAGCTCTGGAGATAGAAGAGCCTTTCG
ATTTTTGGTCACCAGGTGTC

Downstream 100 bases:

>100_bases
TTACTGGGCCCATTGTCATACGCAACATTTATGTGATGATGGCCTACGCTTTTCGGACGTTGAACAGGGAAAACACTGAA
CCTATATCCACCGAGCCGTT

Product: hypothetical protein

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MERWNNKPQPEASEDSEDLELAEPRPRHYWLYSPGPQASEWDEFSSESIMAIGWDDLDDLSTYPSREAIRQALNVDGAGG
SQTNATLALWQFQNDMTVGDIVYAKEGRQKIVGRGEITSNARFEPERESYRHVRSVKWTHIGKWDHPGDAVTKTLTDITK
DHDYVEKLEALITDEVDTEQLVSPSTAPIYDKAAFLSEVYLSETRYVRLRSLLGRKKNVILAGPPGVGKTYAAKRLAYSI
MGTKDPSRVQMIQFHQSYSYEDFMMGYRPTENGGFIISEGPFYRFCEKARADDADRPYFFIIDEINRGNISKIFGELLML
IESDKRGQELRLLYKNETFSIPANVHIIGMMNTADRSLAVLDYALRRRFGFFELAPAYESAGFAQWIQSADNPMLNRLVS
VILDLNREIIDDPTLGQGFAIGHSFLSQPVGNADEAWLYSVVEDEIIPLLNEYWFDEPVKAEQWADKLRAVVA

Sequences:

>Translated_473_residues
MERWNNKPQPEASEDSEDLELAEPRPRHYWLYSPGPQASEWDEFSSESIMAIGWDDLDDLSTYPSREAIRQALNVDGAGG
SQTNATLALWQFQNDMTVGDIVYAKEGRQKIVGRGEITSNARFEPERESYRHVRSVKWTHIGKWDHPGDAVTKTLTDITK
DHDYVEKLEALITDEVDTEQLVSPSTAPIYDKAAFLSEVYLSETRYVRLRSLLGRKKNVILAGPPGVGKTYAAKRLAYSI
MGTKDPSRVQMIQFHQSYSYEDFMMGYRPTENGGFIISEGPFYRFCEKARADDADRPYFFIIDEINRGNISKIFGELLML
IESDKRGQELRLLYKNETFSIPANVHIIGMMNTADRSLAVLDYALRRRFGFFELAPAYESAGFAQWIQSADNPMLNRLVS
VILDLNREIIDDPTLGQGFAIGHSFLSQPVGNADEAWLYSVVEDEIIPLLNEYWFDEPVKAEQWADKLRAVVA
>Mature_473_residues
MERWNNKPQPEASEDSEDLELAEPRPRHYWLYSPGPQASEWDEFSSESIMAIGWDDLDDLSTYPSREAIRQALNVDGAGG
SQTNATLALWQFQNDMTVGDIVYAKEGRQKIVGRGEITSNARFEPERESYRHVRSVKWTHIGKWDHPGDAVTKTLTDITK
DHDYVEKLEALITDEVDTEQLVSPSTAPIYDKAAFLSEVYLSETRYVRLRSLLGRKKNVILAGPPGVGKTYAAKRLAYSI
MGTKDPSRVQMIQFHQSYSYEDFMMGYRPTENGGFIISEGPFYRFCEKARADDADRPYFFIIDEINRGNISKIFGELLML
IESDKRGQELRLLYKNETFSIPANVHIIGMMNTADRSLAVLDYALRRRFGFFELAPAYESAGFAQWIQSADNPMLNRLVS
VILDLNREIIDDPTLGQGFAIGHSFLSQPVGNADEAWLYSVVEDEIIPLLNEYWFDEPVKAEQWADKLRAVVA

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=285, Percent_Identity=48.4210526315789, Blast_Score=265, Evalue=5e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 53891; Mature: 53891

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERWNNKPQPEASEDSEDLELAEPRPRHYWLYSPGPQASEWDEFSSESIMAIGWDDLDDL
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHCCCCCEEEECCCCCHHH
STYPSREAIRQALNVDGAGGSQTNATLALWQFQNDMTVGDIVYAKEGRQKIVGRGEITSN
HCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCHHHCCCCCCCCC
ARFEPERESYRHVRSVKWTHIGKWDHPGDAVTKTLTDITKDHDYVEKLEALITDEVDTEQ
CCCCCHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHH
LVSPSTAPIYDKAAFLSEVYLSETRYVRLRSLLGRKKNVILAGPPGVGKTYAAKRLAYSI
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHH
MGTKDPSRVQMIQFHQSYSYEDFMMGYRPTENGGFIISEGPFYRFCEKARADDADRPYFF
HCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCEEE
IIDEINRGNISKIFGELLMLIESDKRGQELRLLYKNETFSIPANVHIIGMMNTADRSLAV
EEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCEEECCCCEEEEEEECCCCCHHHH
LDYALRRRFGFFELAPAYESAGFAQWIQSADNPMLNRLVSVILDLNREIIDDPTLGQGFA
HHHHHHHHCCCEEECCCHHCCHHHHHHHCCCCHHHHHHHHHHHHHCHHHCCCCCCCCCHH
IGHSFLSQPVGNADEAWLYSVVEDEIIPLLNEYWFDEPVKAEQWADKLRAVVA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MERWNNKPQPEASEDSEDLELAEPRPRHYWLYSPGPQASEWDEFSSESIMAIGWDDLDDL
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHCCCCCEEEECCCCCHHH
STYPSREAIRQALNVDGAGGSQTNATLALWQFQNDMTVGDIVYAKEGRQKIVGRGEITSN
HCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCHHHCCCCCCCCC
ARFEPERESYRHVRSVKWTHIGKWDHPGDAVTKTLTDITKDHDYVEKLEALITDEVDTEQ
CCCCCHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHH
LVSPSTAPIYDKAAFLSEVYLSETRYVRLRSLLGRKKNVILAGPPGVGKTYAAKRLAYSI
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHH
MGTKDPSRVQMIQFHQSYSYEDFMMGYRPTENGGFIISEGPFYRFCEKARADDADRPYFF
HCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCEEE
IIDEINRGNISKIFGELLMLIESDKRGQELRLLYKNETFSIPANVHIIGMMNTADRSLAV
EEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCEEECCCCEEEEEEECCCCCHHHH
LDYALRRRFGFFELAPAYESAGFAQWIQSADNPMLNRLVSVILDLNREIIDDPTLGQGFA
HHHHHHHHCCCEEECCCHHCCHHHHHHHCCCCHHHHHHHHHHHHHCHHHCCCCCCCCCHH
IGHSFLSQPVGNADEAWLYSVVEDEIIPLLNEYWFDEPVKAEQWADKLRAVVA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]