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The map label for this gene is azoR [H]

Identifier: 138897003

GI number: 138897003

Start: 3479743

End: 3480213

Strand: Reverse

Name: azoR [H]

Synonym: GTNG_3374

Alternate gene names: 138897003

Gene position: 3480213-3479743 (Counterclockwise)

Preceding gene: 138897004

Following gene: 138897002

Centisome position: 98.03

GC content: 48.2

Gene sequence:

>471_bases
GTGTTCAGCGGTTGGGGCAAGCTTCGCTCTGGTCAGTCGTTTGAACAGCTGTCGGCCGAAGAAAAAACGAAAGTTGGACG
GATGAATGAGCTGTGCGATCAATTCATCTCCGCTGATAAATACGTGTTCGTCACGCCGATGTGGAACTTTTCCTTCCCAC
CGGTATTGAAAGCATACATTGATGCAGTGGCTGTAGCTGGAAAAACGTTCAAATACACGGCACAAGGACCGATCGGGTTG
CTCACCGATAAAAAAGCGCTCCACATTCAGGCGCGTGGTGGCTTTTATTCGGAAGGCCCAGCAGCACAAATGGAAATGGG
ACACCGTTATTTAGAAATTATCATGCAATTTTTTGGCGTACCATCATTTGAAGGCCTGTTTGTCGAGGGACATGCGGCCG
TGCCGGAAAAAGCGGAAGAAATTAAAGCGAACGCCATTGCTCGGGCAAAAGAATTGGCTCATACATTTTGA

Upstream 100 bases:

>100_bases
CGGGAAGGCATTTATTGAGACATATAAACAAGTGCATCCGGACCATGAGGTCATTCATCTTGACTTATATAAAGAGTATA
TTCCGGAAATCGATGTCGAT

Downstream 100 bases:

>100_bases
TCGAGACAGGCAGGATGTGTTTCTCGATTCTGTTATGATAGTAAATATTGCGCGGGAAATGGCCAACTATTCATCTGGGG
AGGTTCTCTGTTTGGAGAGC

Product: NAD(P)H dehydrogenase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MFSGWGKLRSGQSFEQLSAEEKTKVGRMNELCDQFISADKYVFVTPMWNFSFPPVLKAYIDAVAVAGKTFKYTAQGPIGL
LTDKKALHIQARGGFYSEGPAAQMEMGHRYLEIIMQFFGVPSFEGLFVEGHAAVPEKAEEIKANAIARAKELAHTF

Sequences:

>Translated_156_residues
MFSGWGKLRSGQSFEQLSAEEKTKVGRMNELCDQFISADKYVFVTPMWNFSFPPVLKAYIDAVAVAGKTFKYTAQGPIGL
LTDKKALHIQARGGFYSEGPAAQMEMGHRYLEIIMQFFGVPSFEGLFVEGHAAVPEKAEEIKANAIARAKELAHTF
>Mature_156_residues
MFSGWGKLRSGQSFEQLSAEEKTKVGRMNELCDQFISADKYVFVTPMWNFSFPPVLKAYIDAVAVAGKTFKYTAQGPIGL
LTDKKALHIQARGGFYSEGPAAQMEMGHRYLEIIMQFFGVPSFEGLFVEGHAAVPEKAEEIKANAIARAKELAHTF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=142, Percent_Identity=35.9154929577465, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 17317; Mature: 17317

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSGWGKLRSGQSFEQLSAEEKTKVGRMNELCDQFISADKYVFVTPMWNFSFPPVLKAYI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
DAVAVAGKTFKYTAQGPIGLLTDKKALHIQARGGFYSEGPAAQMEMGHRYLEIIMQFFGV
HHHHHCCCCEEEECCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PSFEGLFVEGHAAVPEKAEEIKANAIARAKELAHTF
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFSGWGKLRSGQSFEQLSAEEKTKVGRMNELCDQFISADKYVFVTPMWNFSFPPVLKAYI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
DAVAVAGKTFKYTAQGPIGLLTDKKALHIQARGGFYSEGPAAQMEMGHRYLEIIMQFFGV
HHHHHCCCCEEEECCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PSFEGLFVEGHAAVPEKAEEIKANAIARAKELAHTF
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA