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The map label for this gene is potH [H]

Identifier: 138896860

GI number: 138896860

Start: 3334497

End: 3335417

Strand: Reverse

Name: potH [H]

Synonym: GTNG_3225

Alternate gene names: 138896860

Gene position: 3335417-3334497 (Counterclockwise)

Preceding gene: 138896861

Following gene: 138896859

Centisome position: 93.95

GC content: 35.4

Gene sequence:

>921_bases
ATGCAGGTCGAAATAAAAACATACCAAAATCAGGAGATACAAAAACAAAAGAAAAGGAAATTAGATAAAAAGCAGTTGGA
GTCTTGGCTACTATTGTTTCCTACTCTTATGATTTTTATCTTTTTCTTCCTATTTCCTTTACTTTCTTTATTTATCACGA
GTTTTAAGACATTTGATCCAAACAGTGGAATTGGGGATAAATGGACATTGGAAAATTACGTGAAGTTTCTTTCAGACCCC
TTTTACCTAGGAGTTATTTGGAGAACGATAAAGATTGGATTGTTAACTACATTATTAACAGTTATCATTTCTTATCCAGT
CGCTTTTCATATTTCTAGAGCCAGTGGGAAGGTTAAAAATTATTTAACATTGATAGTGTTATCACCATTATTGATAAGTA
TGGTCATCAGATGCTATGGGTGGGTTATTTTGTTATCGAATAATGGTGTTGTTAATCGCACTTTGATCAATCTCGGATGG
ATTGATAAACCGCTTACGTTATTGTATACAGAATTAAGCGTTGTGATTGGAATGGTGCATGTACTATTTCCGTATATGGT
ATTAAGCATCATGGGATCCTTAGAAAGGATCGATCCTTCTATTATCAGGGCTTCACAAAGTCTAGGAGCAGGTTTGATTA
GAACATTCTTTTCCATAATATTACCTCTGACATTACCTGGGGTATTTGCCGGTTCAGTTATGGTGTTTAGTTTAAGTGTA
AGCTCATTTGTGACTCCTGCTATTCTCGGCGGTCCACAGGTAAAAGTTATGTCCTATCTAACTTACGAACAAGTTGCAGT
GATATTAAATTGGCCTTATGGGTCTGCCATTGGGTTTGTATTAATTTTTATGGCAACTGTTACGATTATCGTATATAGCA
GAATTCTTGCCCGTTCCGAAAAAGGAGTGGCGATTCAATGA

Upstream 100 bases:

>100_bases
CAGTTTTAAGCCCTGACCAAATTCAGGAAGGGGAAAAAGTATCGATTTTTTGGGATCCAAAAGATTTATTCCTCTCTAAA
AAGTGAGGTGAGTTAACTAG

Downstream 100 bases:

>100_bases
AGAAAAGACTATATAATTTTATATTTCATTCGTTTTGCTTTGTCGTATATGTTTTTTTGTTAGCACCAATTATTGTAGTT
TTACTATCTTCATTGACAAC

Product: ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQVEIKTYQNQEIQKQKKRKLDKKQLESWLLLFPTLMIFIFFFLFPLLSLFITSFKTFDPNSGIGDKWTLENYVKFLSDP
FYLGVIWRTIKIGLLTTLLTVIISYPVAFHISRASGKVKNYLTLIVLSPLLISMVIRCYGWVILLSNNGVVNRTLINLGW
IDKPLTLLYTELSVVIGMVHVLFPYMVLSIMGSLERIDPSIIRASQSLGAGLIRTFFSIILPLTLPGVFAGSVMVFSLSV
SSFVTPAILGGPQVKVMSYLTYEQVAVILNWPYGSAIGFVLIFMATVTIIVYSRILARSEKGVAIQ

Sequences:

>Translated_306_residues
MQVEIKTYQNQEIQKQKKRKLDKKQLESWLLLFPTLMIFIFFFLFPLLSLFITSFKTFDPNSGIGDKWTLENYVKFLSDP
FYLGVIWRTIKIGLLTTLLTVIISYPVAFHISRASGKVKNYLTLIVLSPLLISMVIRCYGWVILLSNNGVVNRTLINLGW
IDKPLTLLYTELSVVIGMVHVLFPYMVLSIMGSLERIDPSIIRASQSLGAGLIRTFFSIILPLTLPGVFAGSVMVFSLSV
SSFVTPAILGGPQVKVMSYLTYEQVAVILNWPYGSAIGFVLIFMATVTIIVYSRILARSEKGVAIQ
>Mature_306_residues
MQVEIKTYQNQEIQKQKKRKLDKKQLESWLLLFPTLMIFIFFFLFPLLSLFITSFKTFDPNSGIGDKWTLENYVKFLSDP
FYLGVIWRTIKIGLLTTLLTVIISYPVAFHISRASGKVKNYLTLIVLSPLLISMVIRCYGWVILLSNNGVVNRTLINLGW
IDKPLTLLYTELSVVIGMVHVLFPYMVLSIMGSLERIDPSIIRASQSLGAGLIRTFFSIILPLTLPGVFAGSVMVFSLSV
SSFVTPAILGGPQVKVMSYLTYEQVAVILNWPYGSAIGFVLIFMATVTIIVYSRILARSEKGVAIQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=236, Percent_Identity=36.0169491525424, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1787369, Length=267, Percent_Identity=32.2097378277154, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1787713, Length=266, Percent_Identity=30.4511278195489, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1789861, Length=287, Percent_Identity=26.4808362369338, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786980, Length=171, Percent_Identity=29.8245614035088, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34439; Mature: 34439

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVEIKTYQNQEIQKQKKRKLDKKQLESWLLLFPTLMIFIFFFLFPLLSLFITSFKTFDP
CEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NSGIGDKWTLENYVKFLSDPFYLGVIWRTIKIGLLTTLLTVIISYPVAFHISRASGKVKN
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHCCHHHH
YLTLIVLSPLLISMVIRCYGWVILLSNNGVVNRTLINLGWIDKPLTLLYTELSVVIGMVH
HHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEHHEEECCCCCCHHHHHHHHHHHHHHHHH
VLFPYMVLSIMGSLERIDPSIIRASQSLGAGLIRTFFSIILPLTLPGVFAGSVMVFSLSV
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSFVTPAILGGPQVKVMSYLTYEQVAVILNWPYGSAIGFVLIFMATVTIIVYSRILARSE
HHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KGVAIQ
CCCCCC
>Mature Secondary Structure
MQVEIKTYQNQEIQKQKKRKLDKKQLESWLLLFPTLMIFIFFFLFPLLSLFITSFKTFDP
CEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NSGIGDKWTLENYVKFLSDPFYLGVIWRTIKIGLLTTLLTVIISYPVAFHISRASGKVKN
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHCCHHHH
YLTLIVLSPLLISMVIRCYGWVILLSNNGVVNRTLINLGWIDKPLTLLYTELSVVIGMVH
HHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEHHEEECCCCCCHHHHHHHHHHHHHHHHH
VLFPYMVLSIMGSLERIDPSIIRASQSLGAGLIRTFFSIILPLTLPGVFAGSVMVFSLSV
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSFVTPAILGGPQVKVMSYLTYEQVAVILNWPYGSAIGFVLIFMATVTIIVYSRILARSE
HHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KGVAIQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]