Click here to switch to the map view.

The map label for this gene is brnQ [H]

Identifier: 138896825

GI number: 138896825

Start: 3291486

End: 3292796

Strand: Reverse

Name: brnQ [H]

Synonym: GTNG_3188

Alternate gene names: 138896825

Gene position: 3292796-3291486 (Counterclockwise)

Preceding gene: 138896826

Following gene: 138896824

Centisome position: 92.75

GC content: 52.56

Gene sequence:

>1311_bases
ATGAAAACGAGAGATGTGCTGTTTACAGGATTGATGCTATTTTCTCTCTTTTTTGGCGCGGGGAACTTGATTTTTCCTCC
ATATTTAGGAATGGAAGCAGGAACGGCGGTATGGCCAGCAATTCTTGGATTTATCGTTACCGGTGTTGGGTTGCCGCTAT
TGTCGGTCGCGGCGGTGGCGTTCGCCAAAGACGGTGTCCGTTCGCTCGGCAACGCGGTCCATCCGGTGTTCAGCCTTTGC
TTTTCGCTCGCTGTGTATTTGGCAATTGGTCCGTTTTTCGGCATTCCGCGCGCGGCCAGCGTTGCCTATGAAATTGGGGT
CAAGCCGTTTTTTTCTGAAAGCGCTTCTTTCGCGATGTGGTTGTTTACTGGTATCTTTTTCGCCCTTGTGTACTGGCTTA
GTTTAAACCCATCGAAGCTTGTCGATCGAATCGGCCAACTATTAACGCCGCTCTTGCTGCTTTCGATTGCTGTGCTTTGC
ATTACCGGTTTGATCCGGCTTGATAACCCGCATGCGGTGCCGGCGGAGAAGTACGCCTCTGCGCCGTTTGCGAAAGGGGT
ACTGGAAGGGTATTTAACGATGGATACGATCGCTGCCCTTGCTTTTGGCATCGTCGTCATCCACGCGCTTCGTGACCGCG
GAGTTAACCAGCCGGCGTCGCAGGCAAAGGCGACGATTCAAGCCGGGATGATCGCCGGATTAGGACTCGCGCTCGTTTAT
ACTGGCATTGCTATCATTGGTGCAAGAATGGCCGATGCCGGCTCATTTGCCAACGGAGCGGAGCTGTTGTCATACGTGTC
ATCGCTGCTGTTTGGCAACGGCGGGAAGTTGTTGTTGGGTGTGATCGTAGCTCTTGCTTGCTTGACGACGGCGGTTGGCC
TTGTTGCCGCTTGTGCGCAATATTTTCAGGAGCTTACACCGACGATTTCGTACCGGACGTATGTCGCTGTGTTGACTGTA
TTTAGCTTTTTGATGTCGAACATAGGGTTAAATGCGCTGATCGCCGTTTCTGTTCCAGTGTTGACGATGATATATCCATT
GGCGATCGTGTTGGTTGCTTTATCGTTTTTCCGCCGCTTTTACCGCGGTTCGACGAAAGTGTATGGTGTTGCGTTGCTCT
TTACCGGCGTGTTTAGCTTGTACGACGGTTTAAAAGCGATCGGGTTGGAAACGGCATGGTTAGAACCGCTCCTCTCGTGG
GCGCCGCTGTTTTCGGTCGGGCTCGGTTGGATCGTACCAGCATTGATCGGTGCTGTGCTCGGCTTTATCATGGACCGGCC
GTCCGCAACGAGACGGAAACATGCTGCGTAA

Upstream 100 bases:

>100_bases
TTAGGATAGTAATATTTCATTATAATCCTAAGTGGCGCGTTTGTTTTGTGATATATCAGAATATTCAATTCATTTTACTA
ACATAGAAATGAGGGATATG

Downstream 100 bases:

>100_bases
GGGACTGACTTAACAGACCCTCCGCTCTCTAGTTAAACAAAAAATGATAGAATAATAAAGGTGTTTGGTGTGTATATATG
GTATTAAAAGGGTGCCTCGT

Product: branched chain amino acid ABC transporter carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKTRDVLFTGLMLFSLFFGAGNLIFPPYLGMEAGTAVWPAILGFIVTGVGLPLLSVAAVAFAKDGVRSLGNAVHPVFSLC
FSLAVYLAIGPFFGIPRAASVAYEIGVKPFFSESASFAMWLFTGIFFALVYWLSLNPSKLVDRIGQLLTPLLLLSIAVLC
ITGLIRLDNPHAVPAEKYASAPFAKGVLEGYLTMDTIAALAFGIVVIHALRDRGVNQPASQAKATIQAGMIAGLGLALVY
TGIAIIGARMADAGSFANGAELLSYVSSLLFGNGGKLLLGVIVALACLTTAVGLVAACAQYFQELTPTISYRTYVAVLTV
FSFLMSNIGLNALIAVSVPVLTMIYPLAIVLVALSFFRRFYRGSTKVYGVALLFTGVFSLYDGLKAIGLETAWLEPLLSW
APLFSVGLGWIVPALIGAVLGFIMDRPSATRRKHAA

Sequences:

>Translated_436_residues
MKTRDVLFTGLMLFSLFFGAGNLIFPPYLGMEAGTAVWPAILGFIVTGVGLPLLSVAAVAFAKDGVRSLGNAVHPVFSLC
FSLAVYLAIGPFFGIPRAASVAYEIGVKPFFSESASFAMWLFTGIFFALVYWLSLNPSKLVDRIGQLLTPLLLLSIAVLC
ITGLIRLDNPHAVPAEKYASAPFAKGVLEGYLTMDTIAALAFGIVVIHALRDRGVNQPASQAKATIQAGMIAGLGLALVY
TGIAIIGARMADAGSFANGAELLSYVSSLLFGNGGKLLLGVIVALACLTTAVGLVAACAQYFQELTPTISYRTYVAVLTV
FSFLMSNIGLNALIAVSVPVLTMIYPLAIVLVALSFFRRFYRGSTKVYGVALLFTGVFSLYDGLKAIGLETAWLEPLLSW
APLFSVGLGWIVPALIGAVLGFIMDRPSATRRKHAA
>Mature_436_residues
MKTRDVLFTGLMLFSLFFGAGNLIFPPYLGMEAGTAVWPAILGFIVTGVGLPLLSVAAVAFAKDGVRSLGNAVHPVFSLC
FSLAVYLAIGPFFGIPRAASVAYEIGVKPFFSESASFAMWLFTGIFFALVYWLSLNPSKLVDRIGQLLTPLLLLSIAVLC
ITGLIRLDNPHAVPAEKYASAPFAKGVLEGYLTMDTIAALAFGIVVIHALRDRGVNQPASQAKATIQAGMIAGLGLALVY
TGIAIIGARMADAGSFANGAELLSYVSSLLFGNGGKLLLGVIVALACLTTAVGLVAACAQYFQELTPTISYRTYVAVLTV
FSFLMSNIGLNALIAVSVPVLTMIYPLAIVLVALSFFRRFYRGSTKVYGVALLFTGVFSLYDGLKAIGLETAWLEPLLSW
APLFSVGLGWIVPALIGAVLGFIMDRPSATRRKHAA

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=440, Percent_Identity=40.2272727272727, Blast_Score=320, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46173; Mature: 46173

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCHHHHCCCC
>Mature Secondary Structure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HHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]