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The map label for this gene is hisH

Identifier: 138896660

GI number: 138896660

Start: 3119030

End: 3119671

Strand: Reverse

Name: hisH

Synonym: GTNG_3023

Alternate gene names: 138896660

Gene position: 3119671-3119030 (Counterclockwise)

Preceding gene: 138896661

Following gene: 138896659

Centisome position: 87.87

GC content: 54.98

Gene sequence:

>642_bases
ATGACGATGATCGGGGTAATCGATTATGGCATGGGCAACTTATATAGCGTCAGCAAAGCGCTCGAGCGGCTCGGCTGCCC
GTATATCGTGAGCGGCGACAAAGAGGAGTTGGCGCGGGTGCGCGGGCTCATTTTGCCTGGGGTTGGTTCGTTCCGCGATG
CGATGCACATTTTACGTGAAACCGGGTTGGCTGATTTTATTCGTTCGGCGGTCCAGGATGGCACGCCGTTGTTAGGCATT
TGTTTAGGGATGCAGTTGCTGTTTGACGAAAGTGAGGAAAACGGACCGACCGAAGGGCTCGGTTTGCTTCGCGGTCGCGT
TGTCCGTTTTCCAGGTGTGACGAATGACGGCGAGCCGTACAAAGTGCCGCATATGGGGTGGAATCGGCTTCGTTTCCATC
GCTCGTCGCCGCTTCTTGACGGCGTCGAGGAAGGGCATGTGTATTTTGTCCATTCGTATTACGTCGTCCCAGGCGAAGAA
GAAGTCGTGCTCGCAAGCAGTGAATACGACGTTGATGTTCCAGCGGTCGTTGGGCGCGACAATGTGTTTGGCACACAGTT
TCATCCGGAAAAAAGCGGCGCGGTCGGCATGAGCATTTTGAACCGTTATGTCGGCATCGTCACAGGAAGGGAGAATGGCT
GA

Upstream 100 bases:

>100_bases
CGATGAAGCGACGATGGTCGACCCGCGCGTCAAAGGCGTCCCGTCGACAAAAGGCATGCTTTGACGCTGCGCTTGACGTA
CCATTGATGAAAGGAATGGG

Downstream 100 bases:

>100_bases
TGGCATCGTTTACGATTTACCCGGCCATCGATATGCGCGGCGGCAAATGTGTTCGCCTGCTGCAAGGTGATTACAACAAA
GAAACGGTGTACGGTGATTC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTMIGVIDYGMGNLYSVSKALERLGCPYIVSGDKEELARVRGLILPGVGSFRDAMHILRETGLADFIRSAVQDGTPLLGI
CLGMQLLFDESEENGPTEGLGLLRGRVVRFPGVTNDGEPYKVPHMGWNRLRFHRSSPLLDGVEEGHVYFVHSYYVVPGEE
EVVLASSEYDVDVPAVVGRDNVFGTQFHPEKSGAVGMSILNRYVGIVTGRENG

Sequences:

>Translated_213_residues
MTMIGVIDYGMGNLYSVSKALERLGCPYIVSGDKEELARVRGLILPGVGSFRDAMHILRETGLADFIRSAVQDGTPLLGI
CLGMQLLFDESEENGPTEGLGLLRGRVVRFPGVTNDGEPYKVPHMGWNRLRFHRSSPLLDGVEEGHVYFVHSYYVVPGEE
EVVLASSEYDVDVPAVVGRDNVFGTQFHPEKSGAVGMSILNRYVGIVTGRENG
>Mature_212_residues
TMIGVIDYGMGNLYSVSKALERLGCPYIVSGDKEELARVRGLILPGVGSFRDAMHILRETGLADFIRSAVQDGTPLLGIC
LGMQLLFDESEENGPTEGLGLLRGRVVRFPGVTNDGEPYKVPHMGWNRLRFHRSSPLLDGVEEGHVYFVHSYYVVPGEEE
VVLASSEYDVDVPAVVGRDNVFGTQFHPEKSGAVGMSILNRYVGIVTGRENG

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=41, Blast_Score=130, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=32.4074074074074, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_GEOTN (A4ISR4)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001127113.1
- ProteinModelPortal:   A4ISR4
- SMR:   A4ISR4
- STRING:   A4ISR4
- GeneID:   4967949
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_3023
- NMPDR:   fig|420246.5.peg.2900
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- PhylomeDB:   A4ISR4
- ProtClustDB:   PRK13141
- BioCyc:   GTHE420246:GTNG_3023-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23302; Mature: 23171

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 81-81 ACT_SITE 188-188 ACT_SITE 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMIGVIDYGMGNLYSVSKALERLGCPYIVSGDKEELARVRGLILPGVGSFRDAMHILRE
CEEEEEEECCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHEECCCCCCHHHHHHHHHH
TGLADFIRSAVQDGTPLLGICLGMQLLFDESEENGPTEGLGLLRGRVVRFPGVTNDGEPY
CCHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHCEEEECCCCCCCCCCE
KVPHMGWNRLRFHRSSPLLDGVEEGHVYFVHSYYVVPGEEEVVLASSEYDVDVPAVVGRD
ECCCCCCHHEEEECCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCEEECCC
NVFGTQFHPEKSGAVGMSILNRYVGIVTGRENG
CCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCC
>Mature Secondary Structure 
TMIGVIDYGMGNLYSVSKALERLGCPYIVSGDKEELARVRGLILPGVGSFRDAMHILRE
EEEEEEECCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHEECCCCCCHHHHHHHHHH
TGLADFIRSAVQDGTPLLGICLGMQLLFDESEENGPTEGLGLLRGRVVRFPGVTNDGEPY
CCHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHCEEEECCCCCCCCCCE
KVPHMGWNRLRFHRSSPLLDGVEEGHVYFVHSYYVVPGEEEVVLASSEYDVDVPAVVGRD
ECCCCCCHHEEEECCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCEEECCC
NVFGTQFHPEKSGAVGMSILNRYVGIVTGRENG
CCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA