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The map label for this gene is hisF [H]

Identifier: 138896658

GI number: 138896658

Start: 3117538

End: 3118296

Strand: Reverse

Name: hisF [H]

Synonym: GTNG_3021

Alternate gene names: 138896658

Gene position: 3118296-3117538 (Counterclockwise)

Preceding gene: 138896659

Following gene: 138896657

Centisome position: 87.83

GC content: 55.6

Gene sequence:

>759_bases
GTGATCACGAAGCGCATCATTCCGTGCTTGGATGTGAAAGACGGCCGCGTTGTCAAAGGGGTGCAGTTCGTTCAGTTGCG
CGATGCTGGTGATCCAGTCGAACTCGCGAAAGCATACGATGAACAAGGGGCGGACGAGCTCGTCTTTTTAGATATTTCCG
CCTCGCACGAAGGACGGAAAACGATGGTTGATGTCGTCGAGCGCGTCGCTGCCCAGCTGGCTATTCCGTTTACAGTTGGC
GGCGGCATTCATTCACTTGACGATATGAAACGCATTTTACGCGCGGGCGCGGACAAAGTGTCGCTCAATACGGCGGCGGT
GCTTCATCCATCGTTAGTAACCGAAGGAGCGGACTTTTTCGGCTCGCAATGCATCGTCGTTGCGATTGATGCGAAATATG
ATGACACGATCGGGTCATGGCGCGTCTATACGCACGGCGGCCGCAATGCGACAGAATGGGAAGTCGTCGCCTGGGCGAAA
GAAGCGGTGCGCCTTGGTGCTGGAGAAATTTTGTTAACGAGCATGGATGCCGACGGCGGGAAAAACGGCTTTGACGTTGA
GTTGACACGGCGAGTGAGCGAAGCGGTCTCCGTTCCGGTTATCGCGTCTGGCGGCGCCGGTAAGGCGGAGCATTTTTTGG
ACGTCTTTGAGCGGGGGAAAGCCGATGCGGCGTTGGCAGCGTCTATTTTCCATTATAAAGAAACGTCCGTCGGGCAAGTG
AAGGCGTACTTAAGAGAGAGGGGGGTCAACGTGCGATGA

Upstream 100 bases:

>100_bases
GCGAATACGCCGAACAAGGCATCGGTGGGGCGATCGTTGGCAAGGCGCTGTACACAAACCAATTTACGCTCGCGGAAGCG
TTAAAGGCGGTGAACGAGCG

Downstream 100 bases:

>100_bases
TAGCGGACATTCGGTTTGACGAGAAGGGGCTTGTGCCTGCCATCGTTCAAGATGCGCAAAGCAAAGAAGTGCTCATGCTT
GCGTATATGAACAAAGAATC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MITKRIIPCLDVKDGRVVKGVQFVQLRDAGDPVELAKAYDEQGADELVFLDISASHEGRKTMVDVVERVAAQLAIPFTVG
GGIHSLDDMKRILRAGADKVSLNTAAVLHPSLVTEGADFFGSQCIVVAIDAKYDDTIGSWRVYTHGGRNATEWEVVAWAK
EAVRLGAGEILLTSMDADGGKNGFDVELTRRVSEAVSVPVIASGGAGKAEHFLDVFERGKADAALAASIFHYKETSVGQV
KAYLRERGVNVR

Sequences:

>Translated_252_residues
MITKRIIPCLDVKDGRVVKGVQFVQLRDAGDPVELAKAYDEQGADELVFLDISASHEGRKTMVDVVERVAAQLAIPFTVG
GGIHSLDDMKRILRAGADKVSLNTAAVLHPSLVTEGADFFGSQCIVVAIDAKYDDTIGSWRVYTHGGRNATEWEVVAWAK
EAVRLGAGEILLTSMDADGGKNGFDVELTRRVSEAVSVPVIASGGAGKAEHFLDVFERGKADAALAASIFHYKETSVGQV
KAYLRERGVNVR
>Mature_252_residues
MITKRIIPCLDVKDGRVVKGVQFVQLRDAGDPVELAKAYDEQGADELVFLDISASHEGRKTMVDVVERVAAQLAIPFTVG
GGIHSLDDMKRILRAGADKVSLNTAAVLHPSLVTEGADFFGSQCIVVAIDAKYDDTIGSWRVYTHGGRNATEWEVVAWAK
EAVRLGAGEILLTSMDADGGKNGFDVELTRRVSEAVSVPVIASGGAGKAEHFLDVFERGKADAALAASIFHYKETSVGQV
KAYLRERGVNVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=51.5625, Blast_Score=256, Evalue=8e-70,
Organism=Escherichia coli, GI87082028, Length=206, Percent_Identity=29.126213592233, Blast_Score=79, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=34.5047923322684, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27098; Mature: 27098

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKRIIPCLDVKDGRVVKGVQFVQLRDAGDPVELAKAYDEQGADELVFLDISASHEGRK
CCCCCCCCCCCCCCCCEECCEEEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHH
TMVDVVERVAAQLAIPFTVGGGIHSLDDMKRILRAGADKVSLNTAAVLHPSLVTEGADFF
HHHHHHHHHHHHEECEEEECCCCCCHHHHHHHHHCCCCEEECCCCEEECCHHHHCCHHHC
GSQCIVVAIDAKYDDTIGSWRVYTHGGRNATEWEVVAWAKEAVRLGAGEILLTSMDADGG
CCCEEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCC
KNGFDVELTRRVSEAVSVPVIASGGAGKAEHFLDVFERGKADAALAASIFHYKETSVGQV
CCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHH
KAYLRERGVNVR
HHHHHHCCCCCC
>Mature Secondary Structure
MITKRIIPCLDVKDGRVVKGVQFVQLRDAGDPVELAKAYDEQGADELVFLDISASHEGRK
CCCCCCCCCCCCCCCCEECCEEEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHH
TMVDVVERVAAQLAIPFTVGGGIHSLDDMKRILRAGADKVSLNTAAVLHPSLVTEGADFF
HHHHHHHHHHHHEECEEEECCCCCCHHHHHHHHHCCCCEEECCCCEEECCHHHHCCHHHC
GSQCIVVAIDAKYDDTIGSWRVYTHGGRNATEWEVVAWAKEAVRLGAGEILLTSMDADGG
CCCEEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCC
KNGFDVELTRRVSEAVSVPVIASGGAGKAEHFLDVFERGKADAALAASIFHYKETSVGQV
CCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHH
KAYLRERGVNVR
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA