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The map label for this gene is ilvB [H]

Identifier: 138896227

GI number: 138896227

Start: 2698836

End: 2700569

Strand: Reverse

Name: ilvB [H]

Synonym: GTNG_2590

Alternate gene names: 138896227

Gene position: 2700569-2698836 (Counterclockwise)

Preceding gene: 138896228

Following gene: 138896226

Centisome position: 76.07

GC content: 55.42

Gene sequence:

>1734_bases
ATGGCAAAGATGAAGGTGGAGGAACAAGCGAAGACGAAGGCGAGAATGAGCGGGTCGCTGATGTTGATTGAAGCGCTCAA
GGCGGAAAATGTCGAAGTCATTTTCGGCTATCCGGGCGGTGCGGTGTTGCCGCTTTACGACGAGCTGTACAAAGCCGGTG
TGTTTCACGTGTTGACGCGCCATGAGCAAGGCGCCATCCATGCGGCGGAAGGGTACGCGCGCATTTCCGGAAAACCTGGG
GTTGTCATCGCCACTTCTGGCCCGGGAGCGACAAACATCGTCACAGGGCTGACGGATGCGATGATGGATTCGCTGCCGCT
CGTTGTCTTTACCGGGCAAGTGGCGACGAACGTCATCGGTTCGGATGCGTTTCAGGAAGCAGACGTTGTCGGGATTACGA
TGCCGATCACAAAACATAACTACCAAGTGCGTGACATCAGCGAACTTCCGAAAATCATCAAAGAAGCGTTTCATATTGCG
ACGACCGGGCGGCCAGGGCCGGTGTTGATTGACATTCCAAAAGATGTGACGATCGCCGAAGGGGAATTTGACTACAACCA
AGATGTCCATCTGCCAGGTTATCAACCGACGACGCAGCCAAACCATTGGCAAATCCGCCGGCTTGTCGAGGCGGTCAGCC
AGTCGAAGCGGCCGGTCATTTTAGCCGGTGCCGGCGTCTTGCACGCCAACGCAGCCGATGAATTGCAGCAATATGCAGAA
CAACAAAACATTCCGGTCGCCCATACGCTCCTTGGCCTCGGTGGTTTCCCAGCTGATCATCCGCTTTTCCTTGGCATGGC
CGGCATGCACGGCACGTATGCGGCGAATATGGCGCTGTATGAGTGCGACTTGCTTATTAACATTGGCGCCCGCTTCGATG
ACCGGGTCACCGGCAATTTAAAATATTTTGCACCAAAGGCGACGGTCGCTCATATTGACATCGATCCAGCGGAAATCGGC
AAAAACGTACCAACGAAAATCCCGATCGTCAGCGATGCGAAGGCGGCCTTGCAAGAGCTGATTGAACAGCAAGGCAAACC
GGCTGACAACGCGGCATGGCTCGCGCAGCTTAATGAATGGAAGCGGCGCTTCCCGCTTCATTACGAGCCGGAAGCCGGGG
CGATCAAACCGCAAAAGCTCATTGAGATGATTTATGAGGTAACGGGCGGCGAAGCGATCGTCACGACCGATGTCGGCCAA
CATCAAATGTGGGCGGCGCAATATTACAAGTTTAATCGGCCGAACCGCTGGGTCACATCCGGGGGACTCGGCACAATGGG
CTTCGGGCTTCCGGCGGCCATCGGTGCCCAGCTTGCTGACCAGAGTGCCACGGTCGTTTCGATCGTCGGCGACGGTGGCT
TCCAAATGACATGCCAAGAGCTGTCGGTCATTCAAGAGCTGCAGCTGCCGATCAAAGTCGTCATCGTCAACAACCAGGCG
CTCGGCATGGTGCGGCAATGGCAGGAACTGTTTTATGACAAACGGTACTCCCATTCACTCATTCCGAACCAGCCGGATTT
CGTGAAATTAGCCGAAGCTTACGGCATGCCGGGGCTACGGGCGAAGACGGAAGCGGAAGCCGCCGAAGTATTAAAACAAG
CATTTGCCATCGACGGTCCGGTGCTGCTTGACTTCCATGTCTGCGCCGACGAAAACGTCTATCCGATGGTCGCGCCCGGA
AAAGGGCTTCATGAAATGGTGGGGGTGAAAGCATGCGAAGAATTATCACAATGA

Upstream 100 bases:

>100_bases
GCGGAAAGGAAACCTTTTCGCCCCTTTGGCTGGGTAAGGCCGGCCATGGAGGGGGAAAAGGTTTTTTATTATGGATGACA
AACAACGGGGAGGATCGACG

Downstream 100 bases:

>100_bases
CCGTCAACAACCGTCCAGGCGTATTAAATCGCATCACCGGGCTGTTTACGAAGCGGCATTACAACATTGAAAGCATCACC
GTCGGCCATACGGAAATCGA

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MAKMKVEEQAKTKARMSGSLMLIEALKAENVEVIFGYPGGAVLPLYDELYKAGVFHVLTRHEQGAIHAAEGYARISGKPG
VVIATSGPGATNIVTGLTDAMMDSLPLVVFTGQVATNVIGSDAFQEADVVGITMPITKHNYQVRDISELPKIIKEAFHIA
TTGRPGPVLIDIPKDVTIAEGEFDYNQDVHLPGYQPTTQPNHWQIRRLVEAVSQSKRPVILAGAGVLHANAADELQQYAE
QQNIPVAHTLLGLGGFPADHPLFLGMAGMHGTYAANMALYECDLLINIGARFDDRVTGNLKYFAPKATVAHIDIDPAEIG
KNVPTKIPIVSDAKAALQELIEQQGKPADNAAWLAQLNEWKRRFPLHYEPEAGAIKPQKLIEMIYEVTGGEAIVTTDVGQ
HQMWAAQYYKFNRPNRWVTSGGLGTMGFGLPAAIGAQLADQSATVVSIVGDGGFQMTCQELSVIQELQLPIKVVIVNNQA
LGMVRQWQELFYDKRYSHSLIPNQPDFVKLAEAYGMPGLRAKTEAEAAEVLKQAFAIDGPVLLDFHVCADENVYPMVAPG
KGLHEMVGVKACEELSQ

Sequences:

>Translated_577_residues
MAKMKVEEQAKTKARMSGSLMLIEALKAENVEVIFGYPGGAVLPLYDELYKAGVFHVLTRHEQGAIHAAEGYARISGKPG
VVIATSGPGATNIVTGLTDAMMDSLPLVVFTGQVATNVIGSDAFQEADVVGITMPITKHNYQVRDISELPKIIKEAFHIA
TTGRPGPVLIDIPKDVTIAEGEFDYNQDVHLPGYQPTTQPNHWQIRRLVEAVSQSKRPVILAGAGVLHANAADELQQYAE
QQNIPVAHTLLGLGGFPADHPLFLGMAGMHGTYAANMALYECDLLINIGARFDDRVTGNLKYFAPKATVAHIDIDPAEIG
KNVPTKIPIVSDAKAALQELIEQQGKPADNAAWLAQLNEWKRRFPLHYEPEAGAIKPQKLIEMIYEVTGGEAIVTTDVGQ
HQMWAAQYYKFNRPNRWVTSGGLGTMGFGLPAAIGAQLADQSATVVSIVGDGGFQMTCQELSVIQELQLPIKVVIVNNQA
LGMVRQWQELFYDKRYSHSLIPNQPDFVKLAEAYGMPGLRAKTEAEAAEVLKQAFAIDGPVLLDFHVCADENVYPMVAPG
KGLHEMVGVKACEELSQ
>Mature_576_residues
AKMKVEEQAKTKARMSGSLMLIEALKAENVEVIFGYPGGAVLPLYDELYKAGVFHVLTRHEQGAIHAAEGYARISGKPGV
VIATSGPGATNIVTGLTDAMMDSLPLVVFTGQVATNVIGSDAFQEADVVGITMPITKHNYQVRDISELPKIIKEAFHIAT
TGRPGPVLIDIPKDVTIAEGEFDYNQDVHLPGYQPTTQPNHWQIRRLVEAVSQSKRPVILAGAGVLHANAADELQQYAEQ
QNIPVAHTLLGLGGFPADHPLFLGMAGMHGTYAANMALYECDLLINIGARFDDRVTGNLKYFAPKATVAHIDIDPAEIGK
NVPTKIPIVSDAKAALQELIEQQGKPADNAAWLAQLNEWKRRFPLHYEPEAGAIKPQKLIEMIYEVTGGEAIVTTDVGQH
QMWAAQYYKFNRPNRWVTSGGLGTMGFGLPAAIGAQLADQSATVVSIVGDGGFQMTCQELSVIQELQLPIKVVIVNNQAL
GMVRQWQELFYDKRYSHSLIPNQPDFVKLAEAYGMPGLRAKTEAEAAEVLKQAFAIDGPVLLDFHVCADENVYPMVAPGK
GLHEMVGVKACEELSQ

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=25.1760563380282, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI21361361, Length=544, Percent_Identity=25, Blast_Score=159, Evalue=7e-39,
Organism=Escherichia coli, GI87081685, Length=564, Percent_Identity=46.9858156028369, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1790104, Length=573, Percent_Identity=43.2809773123909, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1786717, Length=542, Percent_Identity=35.9778597785978, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1787096, Length=539, Percent_Identity=29.1280148423006, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI1788716, Length=481, Percent_Identity=25.7796257796258, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=573, Percent_Identity=26.7015706806283, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=573, Percent_Identity=26.7015706806283, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=576, Percent_Identity=24.1319444444444, Blast_Score=138, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=47.1304347826087, Blast_Score=506, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6320816, Length=484, Percent_Identity=24.3801652892562, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321524, Length=487, Percent_Identity=22.9979466119097, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=491, Percent_Identity=24.0325865580448, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=484, Percent_Identity=23.1404958677686, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922626, Length=484, Percent_Identity=25.4132231404959, Blast_Score=159, Evalue=6e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62662; Mature: 62530

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMKVEEQAKTKARMSGSLMLIEALKAENVEVIFGYPGGAVLPLYDELYKAGVFHVLTR
CCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCEEHHHHHHHHCCHHHHHHC
HEQGAIHAAEGYARISGKPGVVIATSGPGATNIVTGLTDAMMDSLPLVVFTGQVATNVIG
CCCCCEEECCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHC
SDAFQEADVVGITMPITKHNYQVRDISELPKIIKEAFHIATTGRPGPVLIDIPKDVTIAE
CCHHCCCCEEEEEEEEECCCCEECCHHHHHHHHHHHHHEEECCCCCCEEEECCCCCEEEC
GEFDYNQDVHLPGYQPTTQPNHWQIRRLVEAVSQSKRPVILAGAGVLHANAADELQQYAE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEECCCHHHHHHHHH
QQNIPVAHTLLGLGGFPADHPLFLGMAGMHGTYAANMALYECDLLINIGARFDDRVTGNL
HCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCHHHCEEEEEEEEEEEECCCCCCCCCCCE
KYFAPKATVAHIDIDPAEIGKNVPTKIPIVSDAKAALQELIEQQGKPADNAAWLAQLNEW
EEECCCCEEEEEECCHHHHCCCCCCCCCEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KRRFPLHYEPEAGAIKPQKLIEMIYEVTGGEAIVTTDVGQHQMWAAQYYKFNRPNRWVTS
HHHCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEC
GGLGTMGFGLPAAIGAQLADQSATVVSIVGDGGFQMTCQELSVIQELQLPIKVVIVNNQA
CCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCEEHHHHHHHHHHCCCCEEEEEECCCH
LGMVRQWQELFYDKRYSHSLIPNQPDFVKLAEAYGMPGLRAKTEAEAAEVLKQAFAIDGP
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
VLLDFHVCADENVYPMVAPGKGLHEMVGVKACEELSQ
EEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKMKVEEQAKTKARMSGSLMLIEALKAENVEVIFGYPGGAVLPLYDELYKAGVFHVLTR
CCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCEEHHHHHHHHCCHHHHHHC
HEQGAIHAAEGYARISGKPGVVIATSGPGATNIVTGLTDAMMDSLPLVVFTGQVATNVIG
CCCCCEEECCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHC
SDAFQEADVVGITMPITKHNYQVRDISELPKIIKEAFHIATTGRPGPVLIDIPKDVTIAE
CCHHCCCCEEEEEEEEECCCCEECCHHHHHHHHHHHHHEEECCCCCCEEEECCCCCEEEC
GEFDYNQDVHLPGYQPTTQPNHWQIRRLVEAVSQSKRPVILAGAGVLHANAADELQQYAE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEECCCHHHHHHHHH
QQNIPVAHTLLGLGGFPADHPLFLGMAGMHGTYAANMALYECDLLINIGARFDDRVTGNL
HCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCHHHCEEEEEEEEEEEECCCCCCCCCCCE
KYFAPKATVAHIDIDPAEIGKNVPTKIPIVSDAKAALQELIEQQGKPADNAAWLAQLNEW
EEECCCCEEEEEECCHHHHCCCCCCCCCEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KRRFPLHYEPEAGAIKPQKLIEMIYEVTGGEAIVTTDVGQHQMWAAQYYKFNRPNRWVTS
HHHCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEC
GGLGTMGFGLPAAIGAQLADQSATVVSIVGDGGFQMTCQELSVIQELQLPIKVVIVNNQA
CCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCEEHHHHHHHHHHCCCCEEEEEECCCH
LGMVRQWQELFYDKRYSHSLIPNQPDFVKLAEAYGMPGLRAKTEAEAAEVLKQAFAIDGP
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
VLLDFHVCADENVYPMVAPGKGLHEMVGVKACEELSQ
EEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]