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The map label for this gene is pilT [H]

Identifier: 138896195

GI number: 138896195

Start: 2658307

End: 2659362

Strand: Reverse

Name: pilT [H]

Synonym: GTNG_2558

Alternate gene names: 138896195

Gene position: 2659362-2658307 (Counterclockwise)

Preceding gene: 138896196

Following gene: 138896194

Centisome position: 74.9

GC content: 52.27

Gene sequence:

>1056_bases
ATGAAAGAAAAACTTGAGGCGTTATTACGTGCCGCCTTTGAATGCCGTGCGTCCGATGTGCATTTAACTGTCGGCGCACC
GCCGATTTTTCGTGTAGACGGGGAACTGAAAGCATACGGCCATGAGCGGCTTCGTCCGGAAGAGACCGAACAAATGGCAA
AAGCCATCATTCCACCGCCGCTTTGGCAGCAGTTTCAAGAAACAGGGGAGCTCGACTTTTCGTACGGCATAACCGGAGTT
TCCCGCTTCCGTTTAAATGTGTTTAAACAGCGGTCGTGTGTTTCTTTAGCTGTGCGCCTCATTCCGATGCGCATCCCGAC
ACTTGAGGAGCTTGGCATGCCGGCCGTCTTAAAACGCATCGCCGAAAGGCCGCATGGGCTCGTGCTTGTCACCGGGCCGA
CCGGCAGTGGTAAATCGACGACATTGGCAGCCATGATCGACTATATGAACAAAACGATGTCCAAACATATCATTACGCTC
GAAGATCCGATCGAATATTTACATAAACATGGAAAATCGATCATCGACCAGCGTGAAATCGGGTTTGACACCGGCAGCTT
TGCCGCTGGTTTGCGCGCTGCACTTCGCCAAGATCCGGATGTCATTTTAGTCGGGGAAATGCGCGATTTGGAGACGATTC
AAACGGCGATCACCGCCGCGGAAACGGGGCATTTCGTATTAGGCACGCTGCATACGGCAAGCGCGCCGGCGGCGATCGAG
CGGATGATTGACGTTTTTCCGTCGGCCCAGCAGACGCAAATTCGCTTGCAGCTCGCTTCCGTTCTTGTCGCAGTCATCGC
CCAACGTCTGTTTCCGAATAGCCGGGGGAACGGACGAGTGGCAGCGTTCGAAATTTTAATCAATAATTCCGTGGTCGCCA
ACTTAATTCGTAATGAAAAAGAACATCAAATCGTTAACGTGATGCAAACAAGCAAAAACGTTGGCATGCAGACGATGGAA
ATGAGCATCAAAGAGCTTGTGGCCGACGGGCGCATCGATCCACGTGCAGCCGAGGCGTATTTGGTAGGGGAGCGTGGCGA
ACATGGCACAATTTAA

Upstream 100 bases:

>100_bases
GATTTTTTTGCTTGATGACGGGCTATTGAAAGTGAAGCAAGGGATAACGACACTCGAAGAAGTGCTGAAAGTAGCCATTT
TGCATTGATAGGTGAGATCC

Downstream 100 bases:

>100_bases
GTACGAGGGGCGCGATCGGAGCGGGCGAAAAAAGGCGGGGATGATCACCGCCGTCTCGCGGCGCGAGGCAGCCATCAAAC
TGCGGGAAAAAGGGATTCGC

Product: pili biogenesis protein PilT-like ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKEKLEALLRAAFECRASDVHLTVGAPPIFRVDGELKAYGHERLRPEETEQMAKAIIPPPLWQQFQETGELDFSYGITGV
SRFRLNVFKQRSCVSLAVRLIPMRIPTLEELGMPAVLKRIAERPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREIGFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHFVLGTLHTASAPAAIE
RMIDVFPSAQQTQIRLQLASVLVAVIAQRLFPNSRGNGRVAAFEILINNSVVANLIRNEKEHQIVNVMQTSKNVGMQTME
MSIKELVADGRIDPRAAEAYLVGERGEHGTI

Sequences:

>Translated_351_residues
MKEKLEALLRAAFECRASDVHLTVGAPPIFRVDGELKAYGHERLRPEETEQMAKAIIPPPLWQQFQETGELDFSYGITGV
SRFRLNVFKQRSCVSLAVRLIPMRIPTLEELGMPAVLKRIAERPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREIGFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHFVLGTLHTASAPAAIE
RMIDVFPSAQQTQIRLQLASVLVAVIAQRLFPNSRGNGRVAAFEILINNSVVANLIRNEKEHQIVNVMQTSKNVGMQTME
MSIKELVADGRIDPRAAEAYLVGERGEHGTI
>Mature_351_residues
MKEKLEALLRAAFECRASDVHLTVGAPPIFRVDGELKAYGHERLRPEETEQMAKAIIPPPLWQQFQETGELDFSYGITGV
SRFRLNVFKQRSCVSLAVRLIPMRIPTLEELGMPAVLKRIAERPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREIGFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHFVLGTLHTASAPAAIE
RMIDVFPSAQQTQIRLQLASVLVAVIAQRLFPNSRGNGRVAAFEILINNSVVANLIRNEKEHQIVNVMQTSKNVGMQTME
MSIKELVADGRIDPRAAEAYLVGERGEHGTI

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=329, Percent_Identity=45.5927051671732, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1789723, Length=169, Percent_Identity=49.112426035503, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786296, Length=291, Percent_Identity=36.0824742268041, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38722; Mature: 38722

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKLEALLRAAFECRASDVHLTVGAPPIFRVDGELKAYGHERLRPEETEQMAKAIIPPP
CCHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCHHHCCCCCCCCHHHHHHHHHCCCCH
LWQQFQETGELDFSYGITGVSRFRLNVFKQRSCVSLAVRLIPMRIPTLEELGMPAVLKRI
HHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHH
AERPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITLEDPIEYLHKHGKSIIDQREI
HCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHC
GFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHFVLGTLHTASAPAAIE
CCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RMIDVFPSAQQTQIRLQLASVLVAVIAQRLFPNSRGNGRVAAFEILINNSVVANLIRNEK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHCCH
EHQIVNVMQTSKNVGMQTMEMSIKELVADGRIDPRAAEAYLVGERGEHGTI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MKEKLEALLRAAFECRASDVHLTVGAPPIFRVDGELKAYGHERLRPEETEQMAKAIIPPP
CCHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCHHHCCCCCCCCHHHHHHHHHCCCCH
LWQQFQETGELDFSYGITGVSRFRLNVFKQRSCVSLAVRLIPMRIPTLEELGMPAVLKRI
HHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHH
AERPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITLEDPIEYLHKHGKSIIDQREI
HCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHC
GFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHFVLGTLHTASAPAAIE
CCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RMIDVFPSAQQTQIRLQLASVLVAVIAQRLFPNSRGNGRVAAFEILINNSVVANLIRNEK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHCCH
EHQIVNVMQTSKNVGMQTMEMSIKELVADGRIDPRAAEAYLVGERGEHGTI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]