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The map label for this gene is nadB [H]

Identifier: 138896168

GI number: 138896168

Start: 2635952

End: 2637514

Strand: Reverse

Name: nadB [H]

Synonym: GTNG_2531

Alternate gene names: 138896168

Gene position: 2637514-2635952 (Counterclockwise)

Preceding gene: 138896171

Following gene: 138896167

Centisome position: 74.29

GC content: 57.77

Gene sequence:

>1563_bases
GTGGGGGAAAACGGTATCATTATTGTTGGTAGCGGATTGGCCGCTTTAACAGTGGCTTATTATTTACAGGTGCAAGAAAA
TGTGATTGTTTTCACAAAAAAAGGGAAAACGGACTGCAACTCTTGGCGGGCGCAAGGTGGGGTGGCGGCAGCGTTAGCCC
CCGACGATGATTGGCGCGTTCATTTCCACGACACGCTTACAGCCGGCTGCCATCATAACGATGTGCGAATGGTGGAGTTG
CTCGTTCGTGAAGGGCCGAAGCGGCTGCAAGAATGGATGGACGCTGGGATGGCGTTCGATGCAAATGAACATGGACAGCT
TTGCTTCGGCCTTGAAGGCGGGCATAGCCACCGCCGCATTTTACACGCTGGCGGCGACCAAACGGGCAAGGAGATCGTTA
CATTTTTGCTTCAGCAGGTGAAAGGGCGCGTATCAGTGGTGGAAGGTGAACAAGTCATCGAACTTTTGATGGAAGACGGC
CGCTGTGTCGGGGTGAAAACGAAGCGAAAAGACGGTGCTGTCTCCGTTTGGCGGGCGGCGGCAGTCGTCCTAGCAACCGG
CGGCTGCGCCGGGCTGTATACGTTCACTTCCAACGCGCCGACCGTGACTGGTGATGGCATTGCCATGGCGTATCGCGCCG
GCGCGGCAGTCGCCGACATGGAATTTATCCAATTTCATCCGACGATGCTGGCTGTCGGAGGAAAAGCGGTCGGATTAGTA
TCGGAAGCGGTGCGCGGAGAAGGAGCGGTGCTTGAGACGGAAGACGGGCGGCGCTTAATGGATGGCGTTCACCCACTTCG
TGATTTGGCACCGCGTGATGTTGTCGCCCGCGCGATCGCCGCGGAAACGGAGCGAGGCGGCCGCGTCTACTTAAATATTT
CCGCCGTTCCTCATTTCCGCCGCCGTTTTCCGACGATTGCGGCGTTATGCGAGGCGCATGGCGTTGATCTTGAGGTCGGC
CGCCTCCCGGTTGTGCCAGGCGCCCACTTTTTAATGGGCGGCATTATGGTCAATGAATGGGGGCAGACGACCGTGCCGGG
CTTGTATGCCGTTGGGGAAGTGGCGTGCACCGGTGTGCACGGTGCCAACCGGTTGGCAAGCAATTCGTTGCTTGAAGCGA
TCGTCTTTGGAGCCCGTGTGGCCCACTCTATCAATCGCCGGGAGGAGTGGCCGACGTCAGTATACCGCGCTGCCCGACCG
TTTGGCCGCTCGCGCCGTCTTTTGGTGCCTCCGCTTCCGGAACGGTCGTGCATCCGTGAGCGATTGTCCACATTTGTCGG
TATCGTCCGTGAAAAAGGGCGGCTTTGTGAGGCGGTTCGCTGGTTCGAACAATTTTCCTTGCCAGATTGGTTGGATGGCG
ATCTTGAATGCTTGTCGGCAGAGGAGATTGAAACAGGGTATATGCTCCTTACCGGTTGGCTTGTCGCCTCGTCTGCGCTT
CGCCGCACGGAAAGCCGCGGCGGCCACTACCGAAGTGATTTTCCGTATGATCATCCGCACTGGAAAGGTCGACGCCTCGT
GCGGACGAAAGAAGAGTGGGAGCATGCACCAGTTGGACGGTAA

Upstream 100 bases:

>100_bases
TCCCGCCTTTTCTTTCACTTTTTTCAATCAACCTCTTGTCATTAGTTTAAATATGTGTAAATATAGATGTCAAGACAGAT
GAAAAGAGAGGGGAGCGGTT

Downstream 100 bases:

>100_bases
AGCGGGAATAAGATCAAAAGGAGGATGAGGCGATGAATCGGTTAAAACTCGAACAGTTGTTGAGGCAGTTTTTTCTTGAA
GATATCGGCGATGGGGATGT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MGENGIIIVGSGLAALTVAYYLQVQENVIVFTKKGKTDCNSWRAQGGVAAALAPDDDWRVHFHDTLTAGCHHNDVRMVEL
LVREGPKRLQEWMDAGMAFDANEHGQLCFGLEGGHSHRRILHAGGDQTGKEIVTFLLQQVKGRVSVVEGEQVIELLMEDG
RCVGVKTKRKDGAVSVWRAAAVVLATGGCAGLYTFTSNAPTVTGDGIAMAYRAGAAVADMEFIQFHPTMLAVGGKAVGLV
SEAVRGEGAVLETEDGRRLMDGVHPLRDLAPRDVVARAIAAETERGGRVYLNISAVPHFRRRFPTIAALCEAHGVDLEVG
RLPVVPGAHFLMGGIMVNEWGQTTVPGLYAVGEVACTGVHGANRLASNSLLEAIVFGARVAHSINRREEWPTSVYRAARP
FGRSRRLLVPPLPERSCIRERLSTFVGIVREKGRLCEAVRWFEQFSLPDWLDGDLECLSAEEIETGYMLLTGWLVASSAL
RRTESRGGHYRSDFPYDHPHWKGRRLVRTKEEWEHAPVGR

Sequences:

>Translated_520_residues
MGENGIIIVGSGLAALTVAYYLQVQENVIVFTKKGKTDCNSWRAQGGVAAALAPDDDWRVHFHDTLTAGCHHNDVRMVEL
LVREGPKRLQEWMDAGMAFDANEHGQLCFGLEGGHSHRRILHAGGDQTGKEIVTFLLQQVKGRVSVVEGEQVIELLMEDG
RCVGVKTKRKDGAVSVWRAAAVVLATGGCAGLYTFTSNAPTVTGDGIAMAYRAGAAVADMEFIQFHPTMLAVGGKAVGLV
SEAVRGEGAVLETEDGRRLMDGVHPLRDLAPRDVVARAIAAETERGGRVYLNISAVPHFRRRFPTIAALCEAHGVDLEVG
RLPVVPGAHFLMGGIMVNEWGQTTVPGLYAVGEVACTGVHGANRLASNSLLEAIVFGARVAHSINRREEWPTSVYRAARP
FGRSRRLLVPPLPERSCIRERLSTFVGIVREKGRLCEAVRWFEQFSLPDWLDGDLECLSAEEIETGYMLLTGWLVASSAL
RRTESRGGHYRSDFPYDHPHWKGRRLVRTKEEWEHAPVGR
>Mature_519_residues
GENGIIIVGSGLAALTVAYYLQVQENVIVFTKKGKTDCNSWRAQGGVAAALAPDDDWRVHFHDTLTAGCHHNDVRMVELL
VREGPKRLQEWMDAGMAFDANEHGQLCFGLEGGHSHRRILHAGGDQTGKEIVTFLLQQVKGRVSVVEGEQVIELLMEDGR
CVGVKTKRKDGAVSVWRAAAVVLATGGCAGLYTFTSNAPTVTGDGIAMAYRAGAAVADMEFIQFHPTMLAVGGKAVGLVS
EAVRGEGAVLETEDGRRLMDGVHPLRDLAPRDVVARAIAAETERGGRVYLNISAVPHFRRRFPTIAALCEAHGVDLEVGR
LPVVPGAHFLMGGIMVNEWGQTTVPGLYAVGEVACTGVHGANRLASNSLLEAIVFGARVAHSINRREEWPTSVYRAARPF
GRSRRLLVPPLPERSCIRERLSTFVGIVREKGRLCEAVRWFEQFSLPDWLDGDLECLSAEEIETGYMLLTGWLVASSALR
RTESRGGHYRSDFPYDHPHWKGRRLVRTKEEWEHAPVGR

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=549, Percent_Identity=32.9690346083789, Blast_Score=206, Evalue=5e-53,
Organism=Escherichia coli, GI1788928, Length=526, Percent_Identity=38.212927756654, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1786942, Length=544, Percent_Identity=31.25, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1790597, Length=497, Percent_Identity=31.1871227364185, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17505833, Length=565, Percent_Identity=31.1504424778761, Blast_Score=204, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI17550100, Length=557, Percent_Identity=30.7001795332136, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI71986328, Length=457, Percent_Identity=28.4463894967177, Blast_Score=105, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6322416, Length=534, Percent_Identity=32.9588014981273, Blast_Score=206, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6322701, Length=525, Percent_Identity=31.6190476190476, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6320788, Length=478, Percent_Identity=26.9874476987448, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322511, Length=474, Percent_Identity=24.2616033755274, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI17137288, Length=519, Percent_Identity=32.9479768786127, Blast_Score=202, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24655642, Length=519, Percent_Identity=32.9479768786127, Blast_Score=202, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24655647, Length=519, Percent_Identity=32.9479768786127, Blast_Score=202, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24663005, Length=516, Percent_Identity=31.3953488372093, Blast_Score=183, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 56938; Mature: 56807

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGENGIIIVGSGLAALTVAYYLQVQENVIVFTKKGKTDCNSWRAQGGVAAALAPDDDWRV
CCCCCEEEEECCHHHHHHHHHHEEECCEEEEEECCCCCCHHHHCCCCEEEEECCCCCCEE
HFHDTLTAGCHHNDVRMVELLVREGPKRLQEWMDAGMAFDANEHGQLCFGLEGGHSHRRI
EEECCEECCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCEE
LHAGGDQTGKEIVTFLLQQVKGRVSVVEGEQVIELLMEDGRCVGVKTKRKDGAVSVWRAA
EECCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
AVVLATGGCAGLYTFTSNAPTVTGDGIAMAYRAGAAVADMEFIQFHPTMLAVGGKAVGLV
EEEEECCCCCEEEEEECCCCEECCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCHHHHH
SEAVRGEGAVLETEDGRRLMDGVHPLRDLAPRDVVARAIAAETERGGRVYLNISAVPHFR
HHHHCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCCHHH
RRFPTIAALCEAHGVDLEVGRLPVVPGAHFLMGGIMVNEWGQTTVPGLYAVGEVACTGVH
HHCHHHHHHHHHCCCEEECCCCCCCCCHHHHHCCEEEECCCCCCCCCHHHHHHHHEECCC
GANRLASNSLLEAIVFGARVAHSINRREEWPTSVYRAARPFGRSRRLLVPPLPERSCIRE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEECCCCCCHHHHHH
RLSTFVGIVREKGRLCEAVRWFEQFSLPDWLDGDLECLSAEEIETGYMLLTGWLVASSAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHHH
RRTESRGGHYRSDFPYDHPHWKGRRLVRTKEEWEHAPVGR
HHHHHCCCCCCCCCCCCCCCCCCCEECCCHHHHCCCCCCC
>Mature Secondary Structure 
GENGIIIVGSGLAALTVAYYLQVQENVIVFTKKGKTDCNSWRAQGGVAAALAPDDDWRV
CCCCEEEEECCHHHHHHHHHHEEECCEEEEEECCCCCCHHHHCCCCEEEEECCCCCCEE
HFHDTLTAGCHHNDVRMVELLVREGPKRLQEWMDAGMAFDANEHGQLCFGLEGGHSHRRI
EEECCEECCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCEE
LHAGGDQTGKEIVTFLLQQVKGRVSVVEGEQVIELLMEDGRCVGVKTKRKDGAVSVWRAA
EECCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
AVVLATGGCAGLYTFTSNAPTVTGDGIAMAYRAGAAVADMEFIQFHPTMLAVGGKAVGLV
EEEEECCCCCEEEEEECCCCEECCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCHHHHH
SEAVRGEGAVLETEDGRRLMDGVHPLRDLAPRDVVARAIAAETERGGRVYLNISAVPHFR
HHHHCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCCHHH
RRFPTIAALCEAHGVDLEVGRLPVVPGAHFLMGGIMVNEWGQTTVPGLYAVGEVACTGVH
HHCHHHHHHHHHCCCEEECCCCCCCCCHHHHHCCEEEECCCCCCCCCHHHHHHHHEECCC
GANRLASNSLLEAIVFGARVAHSINRREEWPTSVYRAARPFGRSRRLLVPPLPERSCIRE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEECCCCCCHHHHHH
RLSTFVGIVREKGRLCEAVRWFEQFSLPDWLDGDLECLSAEEIETGYMLLTGWLVASSAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHHH
RRTESRGGHYRSDFPYDHPHWKGRRLVRTKEEWEHAPVGR
HHHHHCCCCCCCCCCCCCCCCCCCEECCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]