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The map label for this gene is queA [H]

Identifier: 138896156

GI number: 138896156

Start: 2625034

End: 2626062

Strand: Reverse

Name: queA [H]

Synonym: GTNG_2519

Alternate gene names: 138896156

Gene position: 2626062-2625034 (Counterclockwise)

Preceding gene: 138896157

Following gene: 138896155

Centisome position: 73.97

GC content: 52.48

Gene sequence:

>1029_bases
ATGAAAGTTGACTTGTTTGATTTCCATTTGCCTGAGGAACTCATTGCACAAACGCCGCTCCCGGATCGGGCGGCGTCGCG
GCTGATGGTGCTCGATAAACAAACAGGGGCCATCCGCCATGAAACATTTCGCAACATTATTTCGTATTTACACCCGGGCG
ATTGTCTCGTCTTAAACGACACCCGTGTCATGCCAGCGCGCCTGTACGGGGAAAAAGCGGAAACGGGCGGGATGGTCGAG
GTGCTCTTGTTAAAACAGTTAGACGGCGACCGTTGGGAGACGCTCGTCAAGCCAGGCAAACGGGTCAGACCGGGAACGAA
GCTCGTCTTTGGCGGCGGGAAACTTCAAGCCGTTTGTCTTGATACGCTTGAGCATGGCGGCCGGGTGCTTGAATTTTCGT
ACGAAGGGCTGTTTTATCAAGTATTGGCTGAACTTGGCGAAATGCCGCTCCCGCCGTATATTAAAGAAAAGCTCGATAAC
CCGGAGCGGTATCAGACAGTGTACGCTCGTGAAATCGGCTCAGCGGCAGCGCCAACAGCCGGCCTTCATTTCACTGAGGA
GCTGCTTGACGCCATTCGGGAAAAAGGGATACATATCGCCTTTATTACGCTTCATGTTGGGCTCGGTACATTCCGGCCGG
TGCAAGTGGATGACATCGAAAAACACAATATGCACGCTGAGTTTTATCAAATGAGTGAGGAAACGGCGGCGATATTGAAC
CGCGTGCGTGCGAACGGCGGTCGCATTATCGCCGTCGGTACGACATCGACGAGGACACTTGAGACGATCGCCAATCGGCA
TGATGGCCGGTTTGCCGCTGAGAGCGGTTGGACGGACATTTTCATTTATCCAGGCTATGAGTTTAAAGCCATTGATGGGC
TCGTGACGAACTTCCATTTGCCGAAATCGACACTCATTATGTTAGTGAGTGCATTCGCCGGACGCGAGCATATTTTGCAC
GCGTATGAAGTGGCGGTCAAAGAGCAGTACCGCTTTTTCAGCTTCGGCGATGCGATGCTCATCATTTAA

Upstream 100 bases:

>100_bases
GACCTTTTATTTTCCAATCGTCACATCGATATTGCTAAGCGTCGTATTATCATTGATTTTTTACGTGCTCGGGCGATTTC
GCTAAAGAAAGGAGAACTTT

Downstream 100 bases:

>100_bases
GAAAGGAGAACAGCACGTTGACGACACCGATTCGCTTTGAACTGATCAAAACGTGTCGGCAGACAGGAGCGCGCCTTGGC
ATTCTCCACACGCCGCACGG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKVDLFDFHLPEELIAQTPLPDRAASRLMVLDKQTGAIRHETFRNIISYLHPGDCLVLNDTRVMPARLYGEKAETGGMVE
VLLLKQLDGDRWETLVKPGKRVRPGTKLVFGGGKLQAVCLDTLEHGGRVLEFSYEGLFYQVLAELGEMPLPPYIKEKLDN
PERYQTVYAREIGSAAAPTAGLHFTEELLDAIREKGIHIAFITLHVGLGTFRPVQVDDIEKHNMHAEFYQMSEETAAILN
RVRANGGRIIAVGTTSTRTLETIANRHDGRFAAESGWTDIFIYPGYEFKAIDGLVTNFHLPKSTLIMLVSAFAGREHILH
AYEVAVKEQYRFFSFGDAMLII

Sequences:

>Translated_342_residues
MKVDLFDFHLPEELIAQTPLPDRAASRLMVLDKQTGAIRHETFRNIISYLHPGDCLVLNDTRVMPARLYGEKAETGGMVE
VLLLKQLDGDRWETLVKPGKRVRPGTKLVFGGGKLQAVCLDTLEHGGRVLEFSYEGLFYQVLAELGEMPLPPYIKEKLDN
PERYQTVYAREIGSAAAPTAGLHFTEELLDAIREKGIHIAFITLHVGLGTFRPVQVDDIEKHNMHAEFYQMSEETAAILN
RVRANGGRIIAVGTTSTRTLETIANRHDGRFAAESGWTDIFIYPGYEFKAIDGLVTNFHLPKSTLIMLVSAFAGREHILH
AYEVAVKEQYRFFSFGDAMLII
>Mature_342_residues
MKVDLFDFHLPEELIAQTPLPDRAASRLMVLDKQTGAIRHETFRNIISYLHPGDCLVLNDTRVMPARLYGEKAETGGMVE
VLLLKQLDGDRWETLVKPGKRVRPGTKLVFGGGKLQAVCLDTLEHGGRVLEFSYEGLFYQVLAELGEMPLPPYIKEKLDN
PERYQTVYAREIGSAAAPTAGLHFTEELLDAIREKGIHIAFITLHVGLGTFRPVQVDDIEKHNMHAEFYQMSEETAAILN
RVRANGGRIIAVGTTSTRTLETIANRHDGRFAAESGWTDIFIYPGYEFKAIDGLVTNFHLPKSTLIMLVSAFAGREHILH
AYEVAVKEQYRFFSFGDAMLII

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=351, Percent_Identity=48.7179487179487, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38403; Mature: 38403

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDLFDFHLPEELIAQTPLPDRAASRLMVLDKQTGAIRHETFRNIISYLHPGDCLVLND
CEEEEEECCCCHHHHHCCCCCHHHHCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEC
TRVMPARLYGEKAETGGMVEVLLLKQLDGDRWETLVKPGKRVRPGTKLVFGGGKLQAVCL
CCCCHHHHCCCCCCCCCCEEEEEHHHCCCCHHHHHHCCCCCCCCCCEEEECCCCEEEEEH
DTLEHGGRVLEFSYEGLFYQVLAELGEMPLPPYIKEKLDNPERYQTVYAREIGSAAAPTA
HHHHCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
GLHFTEELLDAIREKGIHIAFITLHVGLGTFRPVQVDDIEKHNMHAEFYQMSEETAAILN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHH
RVRANGGRIIAVGTTSTRTLETIANRHDGRFAAESGWTDIFIYPGYEFKAIDGLVTNFHL
HHHCCCCEEEEEECCCCHHHHHHHCCCCCCEEECCCCEEEEEECCCCEEEHHHHHHCCCC
PKSTLIMLVSAFAGREHILHAYEVAVKEQYRFFSFGDAMLII
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEEECCEEEEC
>Mature Secondary Structure
MKVDLFDFHLPEELIAQTPLPDRAASRLMVLDKQTGAIRHETFRNIISYLHPGDCLVLND
CEEEEEECCCCHHHHHCCCCCHHHHCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEC
TRVMPARLYGEKAETGGMVEVLLLKQLDGDRWETLVKPGKRVRPGTKLVFGGGKLQAVCL
CCCCHHHHCCCCCCCCCCEEEEEHHHCCCCHHHHHHCCCCCCCCCCEEEECCCCEEEEEH
DTLEHGGRVLEFSYEGLFYQVLAELGEMPLPPYIKEKLDNPERYQTVYAREIGSAAAPTA
HHHHCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
GLHFTEELLDAIREKGIHIAFITLHVGLGTFRPVQVDDIEKHNMHAEFYQMSEETAAILN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHH
RVRANGGRIIAVGTTSTRTLETIANRHDGRFAAESGWTDIFIYPGYEFKAIDGLVTNFHL
HHHCCCCEEEEEECCCCHHHHHHHCCCCCCEEECCCCEEEEEECCCCEEEHHHHHHCCCC
PKSTLIMLVSAFAGREHILHAYEVAVKEQYRFFSFGDAMLII
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA