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The map label for this gene is tgt [H]

Identifier: 138896155

GI number: 138896155

Start: 2623874

End: 2625016

Strand: Reverse

Name: tgt [H]

Synonym: GTNG_2518

Alternate gene names: 138896155

Gene position: 2625016-2623874 (Counterclockwise)

Preceding gene: 138896156

Following gene: 138896154

Centisome position: 73.94

GC content: 52.67

Gene sequence:

>1143_bases
TTGACGACACCGATTCGCTTTGAACTGATCAAAACGTGTCGGCAGACAGGAGCGCGCCTTGGCATTCTCCACACGCCGCA
CGGCTCGTTTGAAACACCGATGTTTATGCCAGTCGGGACGCTTGCGACGGTCAAAACGCTATCGCCAGAAGAGTTGAAGG
AAATGGGGGCAGGCGTCATTTTAAGCAATACGTACCATCTTTGGCTGCGCCCAGGCCATGACATTGTCGCTGAGGCGGGC
GGCCTCCACGCTTTTATGAACTGGGATCGCGGCATTTTGACGGATTCCGGTGGATTCCAAGTGTTTAGCTTGAGCGAATT
TCGCCGGATTGAGGAAGAAGGTGTCTATTTCCGCAACCATTTAAACGGCGACAAGCTGTTTTTATCGCCGGAAAAAGCGA
CTGAAATCCAAAATGCGCTCGGTGCTGACATCATCATGGCGTTTGACGAATGTCCGCCGTATCCGGCGACGTATGACTAT
ATGAAACGGTCGGTCGAGCGGACGAGCCGCTGGGCGGAGCGCTGCCTGAAAGCCCATCGACGCTCGAATGAACAAGGGCT
GTTTGGCATCGTCCAAGGCGGTGAATATGAGGACTTACGCCGGCAAAGCGCGCGCGATTTAGTGTCGCTCGATTTTCCTG
GCTATGCCGTCGGTGGGTTGTCGGTCGGCGAGCCGAAAGATGTGATGAATCGGGTGCTTGAGTTTACAACGCCACTTTTG
CCGACGGACAAGCCCCGCTATTTAATGGGCGTCGGTTCACCCGATTCACTCATTGACGGTGCGATCCGCGGCATCGACAT
GTTTGACTGCGTCTTGCCGACGCGCATCGGCCGCAACGGAACGGTTATGACGAGTGAGGGGCGGGTTGTCATTAAAAACG
CCCAGTACGCCCGTGACTTCACACCGCTTGATCCGAACTGCGATTGCTATACGTGTCGAAACTACACGCGCGCCTATATT
CGCCATCTCATTAAATGTGATGAAACATTTGGTATCCGGCTCACGTCTTACCATAACGTCTATTTTTTGATAAAATTAAT
GGAGCAGGTGAGACAAGCGATTCGTGAAGATCGACTCGCCGATTTCCGTGAGGAATTTTTTGAGCGTTACGGCTTTAACA
AGCCAAACGCGAAAAACTTTTAG

Upstream 100 bases:

>100_bases
AGCATATTTTGCACGCGTATGAAGTGGCGGTCAAAGAGCAGTACCGCTTTTTCAGCTTCGGCGATGCGATGCTCATCATT
TAAGAAAGGAGAACAGCACG

Downstream 100 bases:

>100_bases
TCATTAGAAAGGAGGGGGATTGAATGAACGCGACGATTGCGAATTTATTGCCGATCGTGCTGTTTTTCGTCATTTTCTAC
TTTTTGCTTATTCGCCCACA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTTPIRFELIKTCRQTGARLGILHTPHGSFETPMFMPVGTLATVKTLSPEELKEMGAGVILSNTYHLWLRPGHDIVAEAG
GLHAFMNWDRGILTDSGGFQVFSLSEFRRIEEEGVYFRNHLNGDKLFLSPEKATEIQNALGADIIMAFDECPPYPATYDY
MKRSVERTSRWAERCLKAHRRSNEQGLFGIVQGGEYEDLRRQSARDLVSLDFPGYAVGGLSVGEPKDVMNRVLEFTTPLL
PTDKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIGRNGTVMTSEGRVVIKNAQYARDFTPLDPNCDCYTCRNYTRAYI
RHLIKCDETFGIRLTSYHNVYFLIKLMEQVRQAIREDRLADFREEFFERYGFNKPNAKNF

Sequences:

>Translated_380_residues
MTTPIRFELIKTCRQTGARLGILHTPHGSFETPMFMPVGTLATVKTLSPEELKEMGAGVILSNTYHLWLRPGHDIVAEAG
GLHAFMNWDRGILTDSGGFQVFSLSEFRRIEEEGVYFRNHLNGDKLFLSPEKATEIQNALGADIIMAFDECPPYPATYDY
MKRSVERTSRWAERCLKAHRRSNEQGLFGIVQGGEYEDLRRQSARDLVSLDFPGYAVGGLSVGEPKDVMNRVLEFTTPLL
PTDKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIGRNGTVMTSEGRVVIKNAQYARDFTPLDPNCDCYTCRNYTRAYI
RHLIKCDETFGIRLTSYHNVYFLIKLMEQVRQAIREDRLADFREEFFERYGFNKPNAKNF
>Mature_379_residues
TTPIRFELIKTCRQTGARLGILHTPHGSFETPMFMPVGTLATVKTLSPEELKEMGAGVILSNTYHLWLRPGHDIVAEAGG
LHAFMNWDRGILTDSGGFQVFSLSEFRRIEEEGVYFRNHLNGDKLFLSPEKATEIQNALGADIIMAFDECPPYPATYDYM
KRSVERTSRWAERCLKAHRRSNEQGLFGIVQGGEYEDLRRQSARDLVSLDFPGYAVGGLSVGEPKDVMNRVLEFTTPLLP
TDKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIGRNGTVMTSEGRVVIKNAQYARDFTPLDPNCDCYTCRNYTRAYIR
HLIKCDETFGIRLTSYHNVYFLIKLMEQVRQAIREDRLADFREEFFERYGFNKPNAKNF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=366, Percent_Identity=43.4426229508197, Blast_Score=339, Evalue=2e-93,
Organism=Homo sapiens, GI13375872, Length=286, Percent_Identity=25.8741258741259, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=55.1075268817204, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=47.1751412429379, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI25146502, Length=246, Percent_Identity=27.2357723577236, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19920510, Length=372, Percent_Identity=43.5483870967742, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI21355311, Length=255, Percent_Identity=25.8823529411765, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43274; Mature: 43143

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPIRFELIKTCRQTGARLGILHTPHGSFETPMFMPVGTLATVKTLSPEELKEMGAGVI
CCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEECCCCHHHEECCCHHHHHHHCCCEE
LSNTYHLWLRPGHDIVAEAGGLHAFMNWDRGILTDSGGFQVFSLSEFRRIEEEGVYFRNH
EECCEEEEEECCCHHHHCCCCEEEEECCCCCEEECCCCEEEEEHHHHHHHHHCCCEEEEC
LNGDKLFLSPEKATEIQNALGADIIMAFDECPPYPATYDYMKRSVERTSRWAERCLKAHR
CCCCEEEECCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RSNEQGLFGIVQGGEYEDLRRQSARDLVSLDFPGYAVGGLSVGEPKDVMNRVLEFTTPLL
CCCCCCEEEEECCCCHHHHHHHHHHCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCC
PTDKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIGRNGTVMTSEGRVVIKNAQYARDF
CCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCEEEEECCHHHCCC
TPLDPNCDCYTCRNYTRAYIRHLIKCDETFGIRLTSYHNVYFLIKLMEQVRQAIREDRLA
CCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
DFREEFFERYGFNKPNAKNF
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TTPIRFELIKTCRQTGARLGILHTPHGSFETPMFMPVGTLATVKTLSPEELKEMGAGVI
CCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEECCCCHHHEECCCHHHHHHHCCCEE
LSNTYHLWLRPGHDIVAEAGGLHAFMNWDRGILTDSGGFQVFSLSEFRRIEEEGVYFRNH
EECCEEEEEECCCHHHHCCCCEEEEECCCCCEEECCCCEEEEEHHHHHHHHHCCCEEEEC
LNGDKLFLSPEKATEIQNALGADIIMAFDECPPYPATYDYMKRSVERTSRWAERCLKAHR
CCCCEEEECCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RSNEQGLFGIVQGGEYEDLRRQSARDLVSLDFPGYAVGGLSVGEPKDVMNRVLEFTTPLL
CCCCCCEEEEECCCCHHHHHHHHHHCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCC
PTDKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIGRNGTVMTSEGRVVIKNAQYARDF
CCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCEEEEECCHHHCCC
TPLDPNCDCYTCRNYTRAYIRHLIKCDETFGIRLTSYHNVYFLIKLMEQVRQAIREDRLA
CCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
DFREEFFERYGFNKPNAKNF
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA