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The map label for this gene is dnaG [H]

Identifier: 138896057

GI number: 138896057

Start: 2528087

End: 2529886

Strand: Reverse

Name: dnaG [H]

Synonym: GTNG_2420

Alternate gene names: 138896057

Gene position: 2529886-2528087 (Counterclockwise)

Preceding gene: 138896058

Following gene: 138896056

Centisome position: 71.26

GC content: 50.94

Gene sequence:

>1800_bases
ATGGGATATCGTATTCCTGAAGAAACGATTGAAGCGATTCGCCGCAGCGTCGACATTGTTGACGTCATTAGCGAATATGT
TCAACTGAAAAAACAAGGTCGCAACTATTTTGGTTTGTGCCCGTTTCACGGAGAAAAAACGCCATCGTTTTCCGTTTCCC
CGGAGAAACAAATTTTCCACTGCTTCGGCTGTGGGGCGGGAGGGAATGCCTTTACGTTTTTAATGGATATAGAGGGCATT
CCGTTTGTGGAAGCGGCGAAACGTCTTGCGGCTAAAGCAGGCATTGACTTGTCTGCCTATCAGCTGAATGTTCGCGGGCA
TGATGATGGCAAAGCCGGCGAGGCTAAGGTGATGATTGAAGCACACACCTTGCTGAAACGTTTTTACCACCATTTGCTTG
TTTATACAAAAGAAGGACAAGCAGCGCTTGATTACTTGCAGGCGCGCGGATGGACAAAAGAAACAATCGACCGGTTTGAA
ATCGGTTATGCGCCGGATGCGCCCGATGCGGCGGCCAAGTTGCTCGATAGCCATTCCTTTTCACTTCCGATGCTGGAAAA
GGCGGGCTTGGTCACGAAAAAAGAAGACGGACGGTACGTCGACCGCTTCCGAAATCGGATTATGTTTCCGATCCTTGATC
ACCGCGGTGAGACGGTTGGGTTTTCCGGCCGTCTGCTCGGTGAGGGGCAGCCGAAATATGTTAACAGCCCGGAAACACCG
ATTTTCCGTAAAGGCATGCTTTTATATCACTTTCATGAGGCGCGGGTGCCGATACGCAAGCGGCAAGAGGCGTTGTTGTT
TGAAGGGTTTGCGGATGTGATTTCCGCCGTGCAGGCTGGCATTGATTATGCGGTGGCAACGATGGGCACGTCGCTGACCG
AGGAGCAGGCACGCATTTTGCGCCGCCATGCGGAAACGGTCACGATTTGCTACGATGGTGACAACGCCGGAATGGAAGCC
GCTTGGCGCGCCGCAGAGCAACTGAGCGCGCTCGGATGCCGCGTCAAGGTGGCATCCATTCCGAACGGCCTTGATCCGGA
TGAATATATACGCGTTTACGGCAAGGACCGGTTTGTTGGCGAAATTGCTGCTGCTCAGCCGCTTATGGCGTTTAAAATGA
ACCATTTGCGACGGGGGAAAAATTTGCAGCACGAAGGCGACCGGCTTCGTTATATTGAGGAAGTGCTCCGCGAAATCGGC
AAATTGCCGAGTCCTGTCGAAAAAGATTATTATGTACGTCAGCTCGCGGATGAGTTTTCTTTGTCGCTCTCCGCGCTCAA
TGAGCAGCTGTCCCGCTACAAGCAGGAAACGTCAAAGCCGCGGGAAACGTCAGCCAACGAAAAGACCTCACGGCCGACGT
TAACAAAAAAGCTGCTACCGGCTTTCCACAATGCAGAGCGGCTGCTGCTTGCTCATATGATGCGAAGCCGCGATGTGGCA
TTGTTCGTTCAAGAGCGGGTCGGCGGACGTTTTAACATCGAGGAGCATCGGGCGTTAGCCGCTTATATTTATGCTTTTTA
TGAAGAAGGGCATGAAGCTGACGTCGGTGCGCTCATGATTCGGCTTCCTGATGAGTTGCAGTCGTTAGCGAGTGAGCTGT
CGCTTCTGTTGGTGACGGATGACGTCTCCGAGCAAGAGCTTGCCGATTATATTAAACATGTGTTGAATCACCCGAAATGG
TTAATGCTAAAGGAAAAAGAGCAAGAAAAAATTGAAGCGGAGCGAAGAAAAGACTTTTTGACGGCCGCCCGCATCGCCAA
AGAAATGATTGAAATGAAAAAAATGTTATCTTCTTCATAA

Upstream 100 bases:

>100_bases
GAATAAGAGTCGGTTACCCATGTGAAGCCATGGAAGAAGCTCTGTAGTACACAAGACCCCCATGGCTTTAGCCATCAAGG
AGGTTCAGGGGGTTGTTCAT

Downstream 100 bases:

>100_bases
CATGGTGGTATGAGTTTTTAAAGGAGGTAATGAAATGGCGGAAAAACCAGCCCAATCAAAGCAGGCGGAAGCTGCTGCCG
ACACGCTTGAGCAAGCGAAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MGYRIPEETIEAIRRSVDIVDVISEYVQLKKQGRNYFGLCPFHGEKTPSFSVSPEKQIFHCFGCGAGGNAFTFLMDIEGI
PFVEAAKRLAAKAGIDLSAYQLNVRGHDDGKAGEAKVMIEAHTLLKRFYHHLLVYTKEGQAALDYLQARGWTKETIDRFE
IGYAPDAPDAAAKLLDSHSFSLPMLEKAGLVTKKEDGRYVDRFRNRIMFPILDHRGETVGFSGRLLGEGQPKYVNSPETP
IFRKGMLLYHFHEARVPIRKRQEALLFEGFADVISAVQAGIDYAVATMGTSLTEEQARILRRHAETVTICYDGDNAGMEA
AWRAAEQLSALGCRVKVASIPNGLDPDEYIRVYGKDRFVGEIAAAQPLMAFKMNHLRRGKNLQHEGDRLRYIEEVLREIG
KLPSPVEKDYYVRQLADEFSLSLSALNEQLSRYKQETSKPRETSANEKTSRPTLTKKLLPAFHNAERLLLAHMMRSRDVA
LFVQERVGGRFNIEEHRALAAYIYAFYEEGHEADVGALMIRLPDELQSLASELSLLLVTDDVSEQELADYIKHVLNHPKW
LMLKEKEQEKIEAERRKDFLTAARIAKEMIEMKKMLSSS

Sequences:

>Translated_599_residues
MGYRIPEETIEAIRRSVDIVDVISEYVQLKKQGRNYFGLCPFHGEKTPSFSVSPEKQIFHCFGCGAGGNAFTFLMDIEGI
PFVEAAKRLAAKAGIDLSAYQLNVRGHDDGKAGEAKVMIEAHTLLKRFYHHLLVYTKEGQAALDYLQARGWTKETIDRFE
IGYAPDAPDAAAKLLDSHSFSLPMLEKAGLVTKKEDGRYVDRFRNRIMFPILDHRGETVGFSGRLLGEGQPKYVNSPETP
IFRKGMLLYHFHEARVPIRKRQEALLFEGFADVISAVQAGIDYAVATMGTSLTEEQARILRRHAETVTICYDGDNAGMEA
AWRAAEQLSALGCRVKVASIPNGLDPDEYIRVYGKDRFVGEIAAAQPLMAFKMNHLRRGKNLQHEGDRLRYIEEVLREIG
KLPSPVEKDYYVRQLADEFSLSLSALNEQLSRYKQETSKPRETSANEKTSRPTLTKKLLPAFHNAERLLLAHMMRSRDVA
LFVQERVGGRFNIEEHRALAAYIYAFYEEGHEADVGALMIRLPDELQSLASELSLLLVTDDVSEQELADYIKHVLNHPKW
LMLKEKEQEKIEAERRKDFLTAARIAKEMIEMKKMLSSS
>Mature_598_residues
GYRIPEETIEAIRRSVDIVDVISEYVQLKKQGRNYFGLCPFHGEKTPSFSVSPEKQIFHCFGCGAGGNAFTFLMDIEGIP
FVEAAKRLAAKAGIDLSAYQLNVRGHDDGKAGEAKVMIEAHTLLKRFYHHLLVYTKEGQAALDYLQARGWTKETIDRFEI
GYAPDAPDAAAKLLDSHSFSLPMLEKAGLVTKKEDGRYVDRFRNRIMFPILDHRGETVGFSGRLLGEGQPKYVNSPETPI
FRKGMLLYHFHEARVPIRKRQEALLFEGFADVISAVQAGIDYAVATMGTSLTEEQARILRRHAETVTICYDGDNAGMEAA
WRAAEQLSALGCRVKVASIPNGLDPDEYIRVYGKDRFVGEIAAAQPLMAFKMNHLRRGKNLQHEGDRLRYIEEVLREIGK
LPSPVEKDYYVRQLADEFSLSLSALNEQLSRYKQETSKPRETSANEKTSRPTLTKKLLPAFHNAERLLLAHMMRSRDVAL
FVQERVGGRFNIEEHRALAAYIYAFYEEGHEADVGALMIRLPDELQSLASELSLLLVTDDVSEQELADYIKHVLNHPKWL
MLKEKEQEKIEAERRKDFLTAARIAKEMIEMKKMLSSS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=592, Percent_Identity=34.4594594594595, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 67880; Mature: 67748

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYRIPEETIEAIRRSVDIVDVISEYVQLKKQGRNYFGLCPFHGEKTPSFSVSPEKQIFH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHEEE
CFGCGAGGNAFTFLMDIEGIPFVEAAKRLAAKAGIDLSAYQLNVRGHDDGKAGEAKVMIE
EECCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEH
AHTLLKRFYHHLLVYTKEGQAALDYLQARGWTKETIDRFEIGYAPDAPDAAAKLLDSHSF
HHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCC
SLPMLEKAGLVTKKEDGRYVDRFRNRIMFPILDHRGETVGFSGRLLGEGQPKYVNSPETP
CCCCHHHCCCCEECCCCHHHHHHHCCEECEECCCCCCEECCCCEEECCCCCCCCCCCCCC
IFRKGMLLYHFHEARVPIRKRQEALLFEGFADVISAVQAGIDYAVATMGTSLTEEQARIL
HHHCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHH
RRHAETVTICYDGDNAGMEAAWRAAEQLSALGCRVKVASIPNGLDPDEYIRVYGKDRFVG
HHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHEEECCCCCHHH
EIAAAQPLMAFKMNHLRRGKNLQHEGDRLRYIEEVLREIGKLPSPVEKDYYVRQLADEFS
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LSLSALNEQLSRYKQETSKPRETSANEKTSRPTLTKKLLPAFHNAERLLLAHMMRSRDVA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LFVQERVGGRFNIEEHRALAAYIYAFYEEGHEADVGALMIRLPDELQSLASELSLLLVTD
EEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCEEEEEEC
DVSEQELADYIKHVLNHPKWLMLKEKEQEKIEAERRKDFLTAARIAKEMIEMKKMLSSS
CCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GYRIPEETIEAIRRSVDIVDVISEYVQLKKQGRNYFGLCPFHGEKTPSFSVSPEKQIFH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHEEE
CFGCGAGGNAFTFLMDIEGIPFVEAAKRLAAKAGIDLSAYQLNVRGHDDGKAGEAKVMIE
EECCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEH
AHTLLKRFYHHLLVYTKEGQAALDYLQARGWTKETIDRFEIGYAPDAPDAAAKLLDSHSF
HHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCC
SLPMLEKAGLVTKKEDGRYVDRFRNRIMFPILDHRGETVGFSGRLLGEGQPKYVNSPETP
CCCCHHHCCCCEECCCCHHHHHHHCCEECEECCCCCCEECCCCEEECCCCCCCCCCCCCC
IFRKGMLLYHFHEARVPIRKRQEALLFEGFADVISAVQAGIDYAVATMGTSLTEEQARIL
HHHCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHH
RRHAETVTICYDGDNAGMEAAWRAAEQLSALGCRVKVASIPNGLDPDEYIRVYGKDRFVG
HHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHEEECCCCCHHH
EIAAAQPLMAFKMNHLRRGKNLQHEGDRLRYIEEVLREIGKLPSPVEKDYYVRQLADEFS
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LSLSALNEQLSRYKQETSKPRETSANEKTSRPTLTKKLLPAFHNAERLLLAHMMRSRDVA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LFVQERVGGRFNIEEHRALAAYIYAFYEEGHEADVGALMIRLPDELQSLASELSLLLVTD
EEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCEEEEEEC
DVSEQELADYIKHVLNHPKWLMLKEKEQEKIEAERRKDFLTAARIAKEMIEMKKMLSSS
CCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10095067; 10745010 [H]