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The map label for this gene is rpoD [H]

Identifier: 138896056

GI number: 138896056

Start: 2526925

End: 2528052

Strand: Reverse

Name: rpoD [H]

Synonym: GTNG_2419

Alternate gene names: 138896056

Gene position: 2528052-2526925 (Counterclockwise)

Preceding gene: 138896057

Following gene: 138896055

Centisome position: 71.21

GC content: 52.84

Gene sequence:

>1128_bases
ATGGCGGAAAAACCAGCCCAATCAAAGCAGGCGGAAGCTGCTGCCGACACGCTTGAGCAAGCGAAAGAGCAGCTCGCCGA
GCTCGGCAAGAAACGCGGCATCCTCACGTACGAGGAGATCGCCGAGCGGCTCTCCGGCTTTGATTTGGACTCCGACCAGA
TGGATGAATATTACGAATACCTTGCTGATCAGGGCATTGAAGTGATCAGCGAATCTGACATTGAGACCGATCCGGATATT
GATGAACTGGTGAAGGAGGAAGAGTTCGACTTAAACGACTTGTCCGTTCCTCCTGGTGTGAAAATCAATGATCCGGTGCG
CATGTACTTAAAAGAAATCGGCCGTGTTCCGCTTTTGTCGGCGGAAGAAGAGATCGAGCTGGCCAAACGGATCGAGCAAG
GCGACGAAGAAGCGAAACGCCGGCTGACAGAAGCGAACCTCCGTCTCGTCGTCAGCATTGCCAAACGGTATGTTGGCCGT
GGTATGCTCTTTCTTGATTTGATCCAAGAAGGAAACATGGGATTGATCAAAGCGGTTGAAAAATTTGACTACCGCAAAGG
CTATAAATTCAGCACGTATGCGACATGGTGGATTCGCCAAGCCATTACAAGAGCGATCGCTGATCAGGCGCGGACGATCC
GCATCCCTGTTCATATGGTTGAGACGATCAATAAACTGATCCGTGTTCAACGGCAGTTGCTCCAAGACCTCGGGCGCGAA
CCAACGCCGGAAGAAATCGCCGAGGAAATGGACTTGACGCCGGAGAAAGTACGGGAAATTTTAAAAATCGCTCAAGAACC
CGTGTCGCTCGAGACGCCGATCGGCGAGGAAGACGACTCGCACCTTGGTGATTTCATCGAAGACCAAGAAGCGACATCGC
CGTCGGAACACGCTGCGTACGAGCTGTTGAAAGAGCAGCTTGAAGATGTCCTTGATACGCTGACAGACCGTGAAGAAAAC
GTGCTCCGCCTTCGCTTCGGGCTGGACGACGGTCGGACGCGGACGTTGGAAGAGGTCGGGAAAGTGTTTGGTGTGACACG
TGAACGCATTCGCCAAATCGAGGCCAAGGCGTTACGCAAGCTCCGCCATCCAAGCCGCAGCAAACGGCTGAAAGACTTTT
TAGAATGA

Upstream 100 bases:

>100_bases
TTTTTGACGGCCGCCCGCATCGCCAAAGAAATGATTGAAATGAAAAAAATGTTATCTTCTTCATAACATGGTGGTATGAG
TTTTTAAAGGAGGTAATGAA

Downstream 100 bases:

>100_bases
TCGCTTTTTTCGTAGTTTACTTCTTCGGAAGTAAACTATTTTCTTTACCTGATGAAAGGTGATTGACGAATGAATCGGCG
ACGACGGGAAACGATTATCC

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MAEKPAQSKQAEAAADTLEQAKEQLAELGKKRGILTYEEIAERLSGFDLDSDQMDEYYEYLADQGIEVISESDIETDPDI
DELVKEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVPLLSAEEEIELAKRIEQGDEEAKRRLTEANLRLVVSIAKRYVGR
GMLFLDLIQEGNMGLIKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGRE
PTPEEIAEEMDLTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPSEHAAYELLKEQLEDVLDTLTDREEN
VLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLE

Sequences:

>Translated_375_residues
MAEKPAQSKQAEAAADTLEQAKEQLAELGKKRGILTYEEIAERLSGFDLDSDQMDEYYEYLADQGIEVISESDIETDPDI
DELVKEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVPLLSAEEEIELAKRIEQGDEEAKRRLTEANLRLVVSIAKRYVGR
GMLFLDLIQEGNMGLIKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGRE
PTPEEIAEEMDLTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPSEHAAYELLKEQLEDVLDTLTDREEN
VLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLE
>Mature_374_residues
AEKPAQSKQAEAAADTLEQAKEQLAELGKKRGILTYEEIAERLSGFDLDSDQMDEYYEYLADQGIEVISESDIETDPDID
ELVKEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVPLLSAEEEIELAKRIEQGDEEAKRRLTEANLRLVVSIAKRYVGRG
MLFLDLIQEGNMGLIKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREP
TPEEIAEEMDLTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPSEHAAYELLKEQLEDVLDTLTDREENV
LRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=260, Percent_Identity=64.6153846153846, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1789098, Length=301, Percent_Identity=43.8538205980066, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=31.9852941176471, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=29.7029702970297, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 43126; Mature: 42995

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKPAQSKQAEAAADTLEQAKEQLAELGKKRGILTYEEIAERLSGFDLDSDQMDEYYEY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
LADQGIEVISESDIETDPDIDELVKEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVPLLS
HHHCCHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
AEEEIELAKRIEQGDEEAKRRLTEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVE
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHH
KFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGRE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
PTPEEIAEEMDLTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPSEHAAY
CCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
ELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRK
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRHPSRSKRLKDFLE
HHCCHHHHHHHHHHC
>Mature Secondary Structure 
AEKPAQSKQAEAAADTLEQAKEQLAELGKKRGILTYEEIAERLSGFDLDSDQMDEYYEY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
LADQGIEVISESDIETDPDIDELVKEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVPLLS
HHHCCHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
AEEEIELAKRIEQGDEEAKRRLTEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVE
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHH
KFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGRE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
PTPEEIAEEMDLTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPSEHAAY
CCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
ELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRK
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRHPSRSKRLKDFLE
HHCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987246; 3086839; 8969508; 9384377; 2166045; 8363585 [H]