Click here to switch to the map view.

The map label for this gene is iolD

Identifier: 138895462

GI number: 138895462

Start: 1919985

End: 1921904

Strand: Reverse

Name: iolD

Synonym: GTNG_1810

Alternate gene names: 138895462

Gene position: 1921904-1919985 (Counterclockwise)

Preceding gene: 138895463

Following gene: 138895461

Centisome position: 54.13

GC content: 44.27

Gene sequence:

>1920_bases
GTGAGTACCATTCGGTTAACCACTGCACAAGCTCTTGTTAAATTTTTGAATAATCAATATGTAGAGTTTGATGGGAAACA
ACAAAGATTTGTAAAAGGAATCTTTACTATTTTTGGTCATGGCAATGTGCTTGGTCTCGGTCAAGCACTTGAAGAAGATC
CGGGGGAATTAGAAGTATATCAAGGGCGAAATGAACAGGGAATGGCTAACGCGGCGATTGCATTTGCAAAACAAAAAAAC
CGTAAGCAAATTATGGCCGCTACTTCATCTGTCGGTCCTGGTGCAGCGAATATGGTCACATCCGCCGCAACAGCAACGGC
AAATAATATCCCAGTACTTTTGCTTCCTGGTGATGTATTTGCAACTCGTCAGCCTGATCCCGTTTTACAACAAATTGAAC
ACACGCATGATTTGTCTATATCTACCAATGATGCTTTCCGTGCAGTTAGCAAGTATTGGGATCGTGTTTGCCGTCCGGAG
CAACTCATGTCTGCCATGTTGAATGCCATGCGTGTTTTGACAAATCCTGCTGATACAGGTGCTGTAACCATCGCTCTTCC
TCAAGATGTTCAGGGAGAAGCGTGGGATTTTCCTGAAAGCTTTTTTGCCAAGCGTGTCCATCGGATTGAACGTCGACAAC
CGTCTCTCGAAAGTGTTCGAGATGCAGTGAAACTCATTCGTTCCAAGAAAAAGCCGTTGCTCGTTTTAGGTGGTGGCGTT
CGTTATTCAGAGGCAGCTGACGCTTTCGTGAAATTTGCAGAAAAGTTCAACATTCCATTTGCTGAAACTCAAGCTGGAAA
AGGTACGATTGAAAGCTCTCACCCTCTTAATCTAGGAGGGATAGGGGTGACGGGGAACTTAGCGGCTAACACCATTGCCA
AACAGGCGGATTTAATTATTGGAGTCGGTACTCGTTTCACGGATTTTACGACTGCTTCTAAACAGTTGTTCAGCAGTGCG
GAAATTTTAACGATCAATGTTTCAGAATTCCATGCGTCGAAATTAGACGCGGTGAAGGTAGTTGCTGATGCAAAGGCTGG
ACTTGAGGCCATAGCTGAAGCGTTGGGGGATTATGTTTCTGCTTATGGAAATGAGATTTCCGAAGCGAAGCAAGCATGGA
ACAGAGAATTAGAACGTCTTTGCTCTGTTGCCTATGGAGAAAACTTTACACCGGAAATTGCTGGCCACTTAGATGAAAAA
CTGCCTGAATATAGAGAAGCGTTTGGTTCGGAATTGACACAAACCGGCGTAATTGGCAAAGTCAATGAATTGATTGATGA
TGACGCTGTGATTGTAGGGGCTTCGGGCAGCCTTCCTGGTGACCTGCAGCGAATGTGGGTGTGCAAAGACCGCAACACCT
ATCATATGGAATATGGTTATTCTTGCATGGGCTACGAAATCGCTGGGGCGTTTGGTGTCAAGCTTGCTGAACCGGATAAA
GAAGTGTATGCAATGGTTGGAGATGGAAGCTTTTTAATGCTCCACTCTGAACTGGTAACAAGCCTGCAGGAAGGGCAGAA
AATTAATATTATCTTGTTTGATAACTCTGGGTTTGGATGTATCAACAACTTGCAAATGGAAAATGGCATGGGCAGCTTTG
TTACCGAATTCCGTAAACGGAACCTGGAGACCGGGCAACTCGATGGGCCTATTATGACGATTGATTATGCAAAAGTGGCA
GAGGGCTATGGTCTAAAAACATATTCTGTCCGTACAATGGAAGAACTTGAGACGGCTCTTATCGACTCCAAAAAGCAATC
CATCAGCACACTCATCGATATTAAAGTGCTGCCTAAAACAATGACACATGGCTATGGATCTTGGTGGCACGTTGGCGTTG
CAGAAGTGTCGACAAAAGAAAGTGTTCAAGCTGCCTATCGAAATAAACAAGAAAATCTTAAACTAGCACGCAAATACTGA

Upstream 100 bases:

>100_bases
AGAAGAAGTTAATCCAGCAGCTGCTATCTAATCGTTGTTGGTGAAAGCGGATTCGTAGAGAACCTATATTCCGGACAATG
GAAGAGGAGGTTAGAAAATA

Downstream 100 bases:

>100_bases
TATAGCATGCGCTGCAAATAGGGATTAAAGGGGGGAGTTAGAGTTCTGTCCGCAGCTTAGAAGAAAACGGAGGGAAGGGG
AATATGTTCAAAGAAAACAA

Product: IolD protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MSTIRLTTAQALVKFLNNQYVEFDGKQQRFVKGIFTIFGHGNVLGLGQALEEDPGELEVYQGRNEQGMANAAIAFAKQKN
RKQIMAATSSVGPGAANMVTSAATATANNIPVLLLPGDVFATRQPDPVLQQIEHTHDLSISTNDAFRAVSKYWDRVCRPE
QLMSAMLNAMRVLTNPADTGAVTIALPQDVQGEAWDFPESFFAKRVHRIERRQPSLESVRDAVKLIRSKKKPLLVLGGGV
RYSEAADAFVKFAEKFNIPFAETQAGKGTIESSHPLNLGGIGVTGNLAANTIAKQADLIIGVGTRFTDFTTASKQLFSSA
EILTINVSEFHASKLDAVKVVADAKAGLEAIAEALGDYVSAYGNEISEAKQAWNRELERLCSVAYGENFTPEIAGHLDEK
LPEYREAFGSELTQTGVIGKVNELIDDDAVIVGASGSLPGDLQRMWVCKDRNTYHMEYGYSCMGYEIAGAFGVKLAEPDK
EVYAMVGDGSFLMLHSELVTSLQEGQKINIILFDNSGFGCINNLQMENGMGSFVTEFRKRNLETGQLDGPIMTIDYAKVA
EGYGLKTYSVRTMEELETALIDSKKQSISTLIDIKVLPKTMTHGYGSWWHVGVAEVSTKESVQAAYRNKQENLKLARKY

Sequences:

>Translated_639_residues
MSTIRLTTAQALVKFLNNQYVEFDGKQQRFVKGIFTIFGHGNVLGLGQALEEDPGELEVYQGRNEQGMANAAIAFAKQKN
RKQIMAATSSVGPGAANMVTSAATATANNIPVLLLPGDVFATRQPDPVLQQIEHTHDLSISTNDAFRAVSKYWDRVCRPE
QLMSAMLNAMRVLTNPADTGAVTIALPQDVQGEAWDFPESFFAKRVHRIERRQPSLESVRDAVKLIRSKKKPLLVLGGGV
RYSEAADAFVKFAEKFNIPFAETQAGKGTIESSHPLNLGGIGVTGNLAANTIAKQADLIIGVGTRFTDFTTASKQLFSSA
EILTINVSEFHASKLDAVKVVADAKAGLEAIAEALGDYVSAYGNEISEAKQAWNRELERLCSVAYGENFTPEIAGHLDEK
LPEYREAFGSELTQTGVIGKVNELIDDDAVIVGASGSLPGDLQRMWVCKDRNTYHMEYGYSCMGYEIAGAFGVKLAEPDK
EVYAMVGDGSFLMLHSELVTSLQEGQKINIILFDNSGFGCINNLQMENGMGSFVTEFRKRNLETGQLDGPIMTIDYAKVA
EGYGLKTYSVRTMEELETALIDSKKQSISTLIDIKVLPKTMTHGYGSWWHVGVAEVSTKESVQAAYRNKQENLKLARKY
>Mature_638_residues
STIRLTTAQALVKFLNNQYVEFDGKQQRFVKGIFTIFGHGNVLGLGQALEEDPGELEVYQGRNEQGMANAAIAFAKQKNR
KQIMAATSSVGPGAANMVTSAATATANNIPVLLLPGDVFATRQPDPVLQQIEHTHDLSISTNDAFRAVSKYWDRVCRPEQ
LMSAMLNAMRVLTNPADTGAVTIALPQDVQGEAWDFPESFFAKRVHRIERRQPSLESVRDAVKLIRSKKKPLLVLGGGVR
YSEAADAFVKFAEKFNIPFAETQAGKGTIESSHPLNLGGIGVTGNLAANTIAKQADLIIGVGTRFTDFTTASKQLFSSAE
ILTINVSEFHASKLDAVKVVADAKAGLEAIAEALGDYVSAYGNEISEAKQAWNRELERLCSVAYGENFTPEIAGHLDEKL
PEYREAFGSELTQTGVIGKVNELIDDDAVIVGASGSLPGDLQRMWVCKDRNTYHMEYGYSCMGYEIAGAFGVKLAEPDKE
VYAMVGDGSFLMLHSELVTSLQEGQKINIILFDNSGFGCINNLQMENGMGSFVTEFRKRNLETGQLDGPIMTIDYAKVAE
GYGLKTYSVRTMEELETALIDSKKQSISTLIDIKVLPKTMTHGYGSWWHVGVAEVSTKESVQAAYRNKQENLKLARKY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG)

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=22.2419928825623, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1790104, Length=606, Percent_Identity=25.2475247524752, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI87081685, Length=577, Percent_Identity=24.7833622183709, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1786717, Length=499, Percent_Identity=24.8496993987976, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787096, Length=522, Percent_Identity=22.2222222222222, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788716, Length=270, Percent_Identity=25.1851851851852, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17531299, Length=504, Percent_Identity=20.8333333333333, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17531301, Length=504, Percent_Identity=20.8333333333333, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323755, Length=584, Percent_Identity=24.1438356164384, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI19922626, Length=590, Percent_Identity=22.5423728813559, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IOLD_GEOTN (A4IPB6)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001125915.1
- ProteinModelPortal:   A4IPB6
- STRING:   A4IPB6
- GeneID:   4966743
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_1810
- NMPDR:   fig|420246.5.peg.1742
- eggNOG:   COG3962
- HOGENOM:   HBG294332
- OMA:   PVIRMRR
- ProtClustDB:   CLSK2304880
- BioCyc:   GTHE420246:GTNG_1810-MONOMER
- HAMAP:   MF_01669
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 69909; Mature: 69778

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIRLTTAQALVKFLNNQYVEFDGKQQRFVKGIFTIFGHGNVLGLGQALEEDPGELEVY
CCEEEEHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCEEEE
QGRNEQGMANAAIAFAKQKNRKQIMAATSSVGPGAANMVTSAATATANNIPVLLLPGDVF
ECCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCEE
ATRQPDPVLQQIEHTHDLSISTNDAFRAVSKYWDRVCRPEQLMSAMLNAMRVLTNPADTG
CCCCCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTIALPQDVQGEAWDFPESFFAKRVHRIERRQPSLESVRDAVKLIRSKKKPLLVLGGGV
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCC
RYSEAADAFVKFAEKFNIPFAETQAGKGTIESSHPLNLGGIGVTGNLAANTIAKQADLII
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCEEE
GVGTRFTDFTTASKQLFSSAEILTINVSEFHASKLDAVKVVADAKAGLEAIAEALGDYVS
ECCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYGNEISEAKQAWNRELERLCSVAYGENFTPEIAGHLDEKLPEYREAFGSELTQTGVIGK
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCHH
VNELIDDDAVIVGASGSLPGDLQRMWVCKDRNTYHMEYGYSCMGYEIAGAFGVKLAEPDK
HHHHCCCCCEEEECCCCCCCCHHEEEEECCCCEEEEECCCEEECEEEECCCCCEECCCCC
EVYAMVGDGSFLMLHSELVTSLQEGQKINIILFDNSGFGCINNLQMENGMGSFVTEFRKR
EEEEEECCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEECCCCHHHHHHHHHC
NLETGQLDGPIMTIDYAKVAEGYGLKTYSVRTMEELETALIDSKKQSISTLIDIKVLPKT
CCCCCCCCCCEEEEEHHHHHCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHEEEEECHH
MTHGYGSWWHVGVAEVSTKESVQAAYRNKQENLKLARKY
HHCCCCCEEEEEHHHCCHHHHHHHHHHCCHHCCHHHCCC
>Mature Secondary Structure 
STIRLTTAQALVKFLNNQYVEFDGKQQRFVKGIFTIFGHGNVLGLGQALEEDPGELEVY
CEEEEHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCEEEE
QGRNEQGMANAAIAFAKQKNRKQIMAATSSVGPGAANMVTSAATATANNIPVLLLPGDVF
ECCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCEE
ATRQPDPVLQQIEHTHDLSISTNDAFRAVSKYWDRVCRPEQLMSAMLNAMRVLTNPADTG
CCCCCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTIALPQDVQGEAWDFPESFFAKRVHRIERRQPSLESVRDAVKLIRSKKKPLLVLGGGV
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCC
RYSEAADAFVKFAEKFNIPFAETQAGKGTIESSHPLNLGGIGVTGNLAANTIAKQADLII
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCEEE
GVGTRFTDFTTASKQLFSSAEILTINVSEFHASKLDAVKVVADAKAGLEAIAEALGDYVS
ECCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYGNEISEAKQAWNRELERLCSVAYGENFTPEIAGHLDEKLPEYREAFGSELTQTGVIGK
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCHH
VNELIDDDAVIVGASGSLPGDLQRMWVCKDRNTYHMEYGYSCMGYEIAGAFGVKLAEPDK
HHHHCCCCCEEEECCCCCCCCHHEEEEECCCCEEEEECCCEEECEEEECCCCCEECCCCC
EVYAMVGDGSFLMLHSELVTSLQEGQKINIILFDNSGFGCINNLQMENGMGSFVTEFRKR
EEEEEECCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEECCCCHHHHHHHHHC
NLETGQLDGPIMTIDYAKVAEGYGLKTYSVRTMEELETALIDSKKQSISTLIDIKVLPKT
CCCCCCCCCCEEEEEHHHHHCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHEEEEECHH
MTHGYGSWWHVGVAEVSTKESVQAAYRNKQENLKLARKY
HHCCCCCEEEEEHHHCCHHHHHHHHHHCCHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA