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The map label for this gene is narI2 [H]

Identifier: 138895371

GI number: 138895371

Start: 1812069

End: 1812773

Strand: Direct

Name: narI2 [H]

Synonym: GTNG_1715

Alternate gene names: 138895371

Gene position: 1812069-1812773 (Clockwise)

Preceding gene: 138895370

Following gene: 138895378

Centisome position: 51.04

GC content: 53.48

Gene sequence:

>705_bases
ATGGCTCGTCAATTTCTCTGGGTAATTGTTCCTTATTTGTCGCTTGTGCTCTTTATTGCTGGCCATATTTGGCGCTACCA
ACGCGACCAGTTCGGCTGGACATCAAAATCAAGCGAACTGCTCGAAAAACGGCAGTTGCGCCTTGGAAGCCTTCTCTTTC
ACTGGGGTATCTTGTTCGTTTTCGTCGGTCACGTGATGGGAATTCTCATTCCTGAATCATTTTATACAGCGTTAGGCATC
ACCGAAGAGATGTACCACGTCATCGCCTTGGCGGGCGGCATCCCAGCCGGCTTGGCCGCGCTCGTCGGTTTAGTGATCCT
TTTCCGTCGCCGCCTCACGGTGAAACGTGTGCGGGCGACAAGCAGCACTGGTGACTGGGTGGCGCTCGGATTTTTAGCCA
TCGTCATCGCCAGCGGACTATCATCGACATTTCTGAACGTCGATTCGCACGGGTTTGACTACCGGACGACGATCGGCCCG
TGGTTCCGTGGCCTTCTTACGTTCCGTCCGAACCCAGCCTATATGGAAACGGTGCCGCTTTGGTTTCAAATCCATATTCT
CGCCGCGTTCGGCTTGTTCGCTTCGTGGCCATTCACCCGTCTCGTTCACGTGTTCAGCTTTCCGATCCGCTACTTGCAGC
GCAGCTATGTCGTTTATCGCAAGCGAATGCCAAAACAAGCAGAACCGTTCAAACGCGCTCAATGA

Upstream 100 bases:

>100_bases
TGGCAGCCGCGGGCAGCCCGTACGTACAGCTATTTGAAACGCTCAACCTTACCTTTACTCAACTCGGCGTCACGCCGATA
CCGAAAGGAAGTGCAACGGC

Downstream 100 bases:

>100_bases
GCGAGCGGCGAGCATTACACCAGGAACGCCAAGCACGGCGATCATGGAGCAGGCGAGGACAAGTTGCCCTCGCCATCTTA
TACGACTGCGGCTCTTCAAC

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MARQFLWVIVPYLSLVLFIAGHIWRYQRDQFGWTSKSSELLEKRQLRLGSLLFHWGILFVFVGHVMGILIPESFYTALGI
TEEMYHVIALAGGIPAGLAALVGLVILFRRRLTVKRVRATSSTGDWVALGFLAIVIASGLSSTFLNVDSHGFDYRTTIGP
WFRGLLTFRPNPAYMETVPLWFQIHILAAFGLFASWPFTRLVHVFSFPIRYLQRSYVVYRKRMPKQAEPFKRAQ

Sequences:

>Translated_234_residues
MARQFLWVIVPYLSLVLFIAGHIWRYQRDQFGWTSKSSELLEKRQLRLGSLLFHWGILFVFVGHVMGILIPESFYTALGI
TEEMYHVIALAGGIPAGLAALVGLVILFRRRLTVKRVRATSSTGDWVALGFLAIVIASGLSSTFLNVDSHGFDYRTTIGP
WFRGLLTFRPNPAYMETVPLWFQIHILAAFGLFASWPFTRLVHVFSFPIRYLQRSYVVYRKRMPKQAEPFKRAQ
>Mature_233_residues
ARQFLWVIVPYLSLVLFIAGHIWRYQRDQFGWTSKSSELLEKRQLRLGSLLFHWGILFVFVGHVMGILIPESFYTALGIT
EEMYHVIALAGGIPAGLAALVGLVILFRRRLTVKRVRATSSTGDWVALGFLAIVIASGLSSTFLNVDSHGFDYRTTIGPW
FRGLLTFRPNPAYMETVPLWFQIHILAAFGLFASWPFTRLVHVFSFPIRYLQRSYVVYRKRMPKQAEPFKRAQ

Specific function: The Nitrate Reductase Enzyme Complex Allows E.Coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Gamma Chain Is A Membrane-Embedded Heme-Iron Unit Resembling Cytochrome B, Which Transfers Electrons From Quinones To The Beta Subunit.

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=222, Percent_Identity=34.6846846846847, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787738, Length=220, Percent_Identity=36.3636363636364, Blast_Score=117, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 26873; Mature: 26742

Theoretical pI: Translated: 11.11; Mature: 11.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQFLWVIVPYLSLVLFIAGHIWRYQRDQFGWTSKSSELLEKRQLRLGSLLFHWGILFV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FVGHVMGILIPESFYTALGITEEMYHVIALAGGIPAGLAALVGLVILFRRRLTVKRVRAT
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
SSTGDWVALGFLAIVIASGLSSTFLNVDSHGFDYRTTIGPWFRGLLTFRPNPAYMETVPL
CCCCCHHHHHHHHHHHHHCHHHHHEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHH
WFQIHILAAFGLFASWPFTRLVHVFSFPIRYLQRSYVVYRKRMPKQAEPFKRAQ
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ARQFLWVIVPYLSLVLFIAGHIWRYQRDQFGWTSKSSELLEKRQLRLGSLLFHWGILFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FVGHVMGILIPESFYTALGITEEMYHVIALAGGIPAGLAALVGLVILFRRRLTVKRVRAT
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
SSTGDWVALGFLAIVIASGLSSTFLNVDSHGFDYRTTIGPWFRGLLTFRPNPAYMETVPL
CCCCCHHHHHHHHHHHHHCHHHHHEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHH
WFQIHILAAFGLFASWPFTRLVHVFSFPIRYLQRSYVVYRKRMPKQAEPFKRAQ
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377; 7557333 [H]