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The map label for this gene is nasF [H]

Identifier: 138895338

GI number: 138895338

Start: 1771682

End: 1772452

Strand: Reverse

Name: nasF [H]

Synonym: GTNG_1682

Alternate gene names: 138895338

Gene position: 1772452-1771682 (Counterclockwise)

Preceding gene: 138895339

Following gene: 138895337

Centisome position: 49.92

GC content: 59.4

Gene sequence:

>771_bases
ATGACCGTCGGCAAAGTGTATTTAGTCGGCGCTGGGCCGGGTGATGAAAAGCTCATCACGGTTTACGGCCGCGAATGCCT
AGAACGGGCGGATGTCATTATTTACGACCGGCTCATCAACCGTAAGCTGCTGCGCTACGCGAAGCCGGACGCTGAGCTTC
TTTACTGCGGCAAAGAGCCGGGAAAACATGACACGGTTCAAGAACAAATTCATGAGCTGCTCGTTTGGCATGCGCAACAA
GGAAAAACGGTCGTCCGCTTAAAAGGCGGCGATCCGTGCATTTTTGGCCGCGTTGGTGAAGAGGCGGAAGTGTTGGTGAA
AGCCGGAATTCCGTTTGAAATCGTTCCAGGGGTGACATCCGGCATCGCTGTGCCGGCGTACGCCGGCATTCCGGTCACCC
ACCGTGATTACGCCGCCTCGGTGGCGATGGTCAGCGGGCATCGGAGTGAGCGGATCGATTGGGAGGCGCTGACTCGAGGA
TGCGATACGATCATTGTCTACATGGGAGCGGCCAACTTGTCGGATATTTGCGGCCGTTTGATGGCGGCCGGAAAGCCGTC
GGATACGCCGGCGGCCATCATTGAATGGGGGACGACCGAGCGGCAGCGCACGGTGGCGGCGACGCTTTCGACGCTGTCAG
ACGAGGCGGAACAGGCGGGCATTTCCCATCCGGCCATCATCATCATCGGCGATGTCGTTCGTCTGCGTGAGACGCTCCAA
TGGTTTCCGGAGCGGCAAGGGGGCGTGGTCGATGCCGACGCCTCGTCGTAG

Upstream 100 bases:

>100_bases
CGTTTCCGCTCTTGATTTCAGCAATGGCGATGGTGAACGAAATGGCGACGTTCGCCTCGGCGGGCATTTCCACGGCCAAT
GCGAAAGGGGAGAAGGGGCG

Downstream 100 bases:

>100_bases
CGGGCGTGTCATCGTTTATACGGGCGACGGAAAAGGAAAAACGACGGCCGCATTGGGGCTTGCCCTGCGTGCCGTCGGCC
GGGGAATGAACGTCGCGGTT

Product: uroporphyrin-III C-methyltransferase-like protein

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTVGKVYLVGAGPGDEKLITVYGRECLERADVIIYDRLINRKLLRYAKPDAELLYCGKEPGKHDTVQEQIHELLVWHAQQ
GKTVVRLKGGDPCIFGRVGEEAEVLVKAGIPFEIVPGVTSGIAVPAYAGIPVTHRDYAASVAMVSGHRSERIDWEALTRG
CDTIIVYMGAANLSDICGRLMAAGKPSDTPAAIIEWGTTERQRTVAATLSTLSDEAEQAGISHPAIIIIGDVVRLRETLQ
WFPERQGGVVDADASS

Sequences:

>Translated_256_residues
MTVGKVYLVGAGPGDEKLITVYGRECLERADVIIYDRLINRKLLRYAKPDAELLYCGKEPGKHDTVQEQIHELLVWHAQQ
GKTVVRLKGGDPCIFGRVGEEAEVLVKAGIPFEIVPGVTSGIAVPAYAGIPVTHRDYAASVAMVSGHRSERIDWEALTRG
CDTIIVYMGAANLSDICGRLMAAGKPSDTPAAIIEWGTTERQRTVAATLSTLSDEAEQAGISHPAIIIIGDVVRLRETLQ
WFPERQGGVVDADASS
>Mature_255_residues
TVGKVYLVGAGPGDEKLITVYGRECLERADVIIYDRLINRKLLRYAKPDAELLYCGKEPGKHDTVQEQIHELLVWHAQQG
KTVVRLKGGDPCIFGRVGEEAEVLVKAGIPFEIVPGVTSGIAVPAYAGIPVTHRDYAASVAMVSGHRSERIDWEALTRGC
DTIIVYMGAANLSDICGRLMAAGKPSDTPAAIIEWGTTERQRTVAATLSTLSDEAEQAGISHPAIIIIGDVVRLRETLQW
FPERQGGVVDADASS

Specific function: Multifunctional Enzyme That Catalyze The Sam-Dependent Methylation Of Uroporphyrinogen III At Position C-2 And C-7 To Form Precorrin-2 And Then Position C-12 Or C-18 To Form Trimethylpyrrocorphin 2. It Also Catalyzes The Conversion Of Precorrin-2 Into Sir

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=241, Percent_Identity=46.8879668049792, Blast_Score=228, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6322922, Length=254, Percent_Identity=30.3149606299213, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 27691; Mature: 27560

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVGKVYLVGAGPGDEKLITVYGRECLERADVIIYDRLINRKLLRYAKPDAELLYCGKEP
CCCCEEEEEECCCCCCCEEEEECHHHHHHCCEEEEHHHHHHHHHHHCCCCCEEEEECCCC
GKHDTVQEQIHELLVWHAQQGKTVVRLKGGDPCIFGRVGEEAEVLVKAGIPFEIVPGVTS
CCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCEEEEEECCCCEEECCCCCC
GIAVPAYAGIPVTHRDYAASVAMVSGHRSERIDWEALTRGCDTIIVYMGAANLSDICGRL
CCCCCCCCCCCCCCCHHHHHHHEECCCCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHH
MAAGKPSDTPAAIIEWGTTERQRTVAATLSTLSDEAEQAGISHPAIIIIGDVVRLRETLQ
HHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHH
WFPERQGGVVDADASS
HCCCCCCCEEECCCCC
>Mature Secondary Structure 
TVGKVYLVGAGPGDEKLITVYGRECLERADVIIYDRLINRKLLRYAKPDAELLYCGKEP
CCCEEEEEECCCCCCCEEEEECHHHHHHCCEEEEHHHHHHHHHHHCCCCCEEEEECCCC
GKHDTVQEQIHELLVWHAQQGKTVVRLKGGDPCIFGRVGEEAEVLVKAGIPFEIVPGVTS
CCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCEEEEEECCCCEEECCCCCC
GIAVPAYAGIPVTHRDYAASVAMVSGHRSERIDWEALTRGCDTIIVYMGAANLSDICGRL
CCCCCCCCCCCCCCCHHHHHHHEECCCCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHH
MAAGKPSDTPAAIIEWGTTERQRTVAATLSTLSDEAEQAGISHPAIIIIGDVVRLRETLQ
HHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHH
WFPERQGGVVDADASS
HCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]