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The map label for this gene is ytsJ [H]

Identifier: 138895047

GI number: 138895047

Start: 1471853

End: 1473079

Strand: Direct

Name: ytsJ [H]

Synonym: GTNG_1385

Alternate gene names: 138895047

Gene position: 1471853-1473079 (Clockwise)

Preceding gene: 138895046

Following gene: 138895048

Centisome position: 41.46

GC content: 55.34

Gene sequence:

>1227_bases
ATGGAAAATTTGCGGGAACGAGCATTACTTGTCCACCGACAGGCAAAAGGAAAACTGTCGGTTGAAGTGAAAGTGCCTGT
TGTGGATGCAAATGACTTAAGCGTCATTTATTCGCCGGGAGTGGCTGAACCGTGCAAAGAAATTTACGCCAACAAAGATG
AGATGTATGACTATACGATAAAAGGCAATTTCGTCGCCGTTGTTTCCAACGGTACTGCGGTGCTCGGGCTCGGCAACATC
GGTGCGCGGGCGGCGCTTCCGGTGATGGAAGGGAAAGCCGCCTTGTTTAAAGCGTTCGCCGGCATTGATGCGGTGCCGCT
TTGCTTAGACACCGAGGACCCAGAACAAATCATCCAAACTGTCAAACTGCTCGAGCCAACATTTGGTGGTATCAATTTGG
AGGATATTTCCGCCCCTGCCTGCTTTATCATTGAAGAGCGGCTGCGCCAAGAAATGGCGATCCCTGTGTTTCACGATGAT
CAGCACGGTACGGCTATTGTCGTCGCCGCTGGGCTGACGAACGCCTTGAAAGTTGTCGGCAAACGGCTCGCCGATTGTCG
GGTTGTCATCAACGGTGCAGGCGCTGCCGGGGTGGCGATCGCCAAACTGCTGTTGTCGATCGGGGTCGGCGACCTCATTG
TTTGTGATACAAAAGGCGCGATTTATGAAGGGCGCCCACACGGTATGAATGCCATGAAAGAGGAATTAGCAAAACAGACG
AATCAGCGCCGTGTTTCCGGGATGCTGGAAGAGGTGATTTCAGGTGCCGACGTGTTTATCGGCGTCTCAGTTGCCGGTGC
GCTGACGCCGGCAATGGTTCGGTCGATGAACCGGGGGGCGATTGTATTCGCCTTGGCCAATCCGGTGCCGGAAATCATGC
CGGATGAGGCGAAAGCCGCAGGCGCTGCCGTCGTGGCAACCGGCCGCTCAGACTTGCCGAACCAAGTGAACAATGTGCTG
GCGTTCCCCGGCATCTTCCGCGGTGCGCTCGATGTGCGGGCGACGCAAATTAACGAGGCGATGAAAATCGCTGCTGCCGA
AGCCATCGCCGGGCTCATTCAGCCGGAAGAACTGACTGAGGAGTACGTCATCCCGAACCCGTTTGACCAACATGTTGTGG
CGGCGGTCGCTTCTGCTGTTGCCCGTGCTGCCATCGAGACCGGGGTGGCTCGGAACAAGGAGAAATATGAGGGAAAAACC
TTGTTAGAGAGGGGTAACCACCAATGA

Upstream 100 bases:

>100_bases
TAGAAAGGGGGCAGCTCCTAGTTGCTACTGGCCGGTTACAGAACGCAAAGCAGCATGAAGGCGGCATAGATGGGACGAGG
TTAGTAGCTAGGGATTGGTT

Downstream 100 bases:

>100_bases
AGTTTGCCCGTTTTATCGCCGCTGGCGAGATTCGCACCGGTGTAGTGAGCGGCGAAGTCATCCGTGAAATTGAAGGAGAC
ATGTTTACGGCTTGGGATTA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MENLRERALLVHRQAKGKLSVEVKVPVVDANDLSVIYSPGVAEPCKEIYANKDEMYDYTIKGNFVAVVSNGTAVLGLGNI
GARAALPVMEGKAALFKAFAGIDAVPLCLDTEDPEQIIQTVKLLEPTFGGINLEDISAPACFIIEERLRQEMAIPVFHDD
QHGTAIVVAAGLTNALKVVGKRLADCRVVINGAGAAGVAIAKLLLSIGVGDLIVCDTKGAIYEGRPHGMNAMKEELAKQT
NQRRVSGMLEEVISGADVFIGVSVAGALTPAMVRSMNRGAIVFALANPVPEIMPDEAKAAGAAVVATGRSDLPNQVNNVL
AFPGIFRGALDVRATQINEAMKIAAAEAIAGLIQPEELTEEYVIPNPFDQHVVAAVASAVARAAIETGVARNKEKYEGKT
LLERGNHQ

Sequences:

>Translated_408_residues
MENLRERALLVHRQAKGKLSVEVKVPVVDANDLSVIYSPGVAEPCKEIYANKDEMYDYTIKGNFVAVVSNGTAVLGLGNI
GARAALPVMEGKAALFKAFAGIDAVPLCLDTEDPEQIIQTVKLLEPTFGGINLEDISAPACFIIEERLRQEMAIPVFHDD
QHGTAIVVAAGLTNALKVVGKRLADCRVVINGAGAAGVAIAKLLLSIGVGDLIVCDTKGAIYEGRPHGMNAMKEELAKQT
NQRRVSGMLEEVISGADVFIGVSVAGALTPAMVRSMNRGAIVFALANPVPEIMPDEAKAAGAAVVATGRSDLPNQVNNVL
AFPGIFRGALDVRATQINEAMKIAAAEAIAGLIQPEELTEEYVIPNPFDQHVVAAVASAVARAAIETGVARNKEKYEGKT
LLERGNHQ
>Mature_408_residues
MENLRERALLVHRQAKGKLSVEVKVPVVDANDLSVIYSPGVAEPCKEIYANKDEMYDYTIKGNFVAVVSNGTAVLGLGNI
GARAALPVMEGKAALFKAFAGIDAVPLCLDTEDPEQIIQTVKLLEPTFGGINLEDISAPACFIIEERLRQEMAIPVFHDD
QHGTAIVVAAGLTNALKVVGKRLADCRVVINGAGAAGVAIAKLLLSIGVGDLIVCDTKGAIYEGRPHGMNAMKEELAKQT
NQRRVSGMLEEVISGADVFIGVSVAGALTPAMVRSMNRGAIVFALANPVPEIMPDEAKAAGAAVVATGRSDLPNQVNNVL
AFPGIFRGALDVRATQINEAMKIAAAEAIAGLIQPEELTEEYVIPNPFDQHVVAAVASAVARAAIETGVARNKEKYEGKT
LLERGNHQ

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=399, Percent_Identity=25.8145363408521, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI62420882, Length=361, Percent_Identity=27.1468144044321, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI62420880, Length=361, Percent_Identity=27.1468144044321, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI239049447, Length=361, Percent_Identity=27.1468144044321, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI4505145, Length=361, Percent_Identity=26.5927977839335, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI270265879, Length=320, Percent_Identity=27.5, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1788806, Length=407, Percent_Identity=51.3513513513513, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17537199, Length=406, Percent_Identity=26.3546798029557, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322823, Length=387, Percent_Identity=28.4237726098191, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281362672, Length=439, Percent_Identity=26.6514806378132, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI281362674, Length=439, Percent_Identity=26.6514806378132, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21356279, Length=453, Percent_Identity=26.4900662251656, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24646388, Length=438, Percent_Identity=27.3972602739726, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24646386, Length=438, Percent_Identity=27.3972602739726, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI78707242, Length=481, Percent_Identity=25.1559251559252, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI78707238, Length=481, Percent_Identity=25.1559251559252, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI78707240, Length=460, Percent_Identity=25.2173913043478, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 43103; Mature: 43103

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLRERALLVHRQAKGKLSVEVKVPVVDANDLSVIYSPGVAEPCKEIYANKDEMYDYTI
CCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEECCCCCHHHHHHHCCCCCCEEEEE
KGNFVAVVSNGTAVLGLGNIGARAALPVMEGKAALFKAFAGIDAVPLCLDTEDPEQIIQT
ECCEEEEEECCEEEEEECCCCCHHCCCHHCCHHHHHHHHHCCCCEEEEECCCCHHHHHHH
VKLLEPTFGGINLEDISAPACFIIEERLRQEMAIPVFHDDQHGTAIVVAAGLTNALKVVG
HHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCHHHHHHHHH
KRLADCRVVINGAGAAGVAIAKLLLSIGVGDLIVCDTKGAIYEGRPHGMNAMKEELAKQT
HHHCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCEECCCCCCHHHHHHHHHHHH
NQRRVSGMLEEVISGADVFIGVSVAGALTPAMVRSMNRGAIVFALANPVPEIMPDEAKAA
HHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHC
GAAVVATGRSDLPNQVNNVLAFPGIFRGALDVRATQINEAMKIAAAEAIAGLIQPEELTE
CEEEEECCCCCCCHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EYVIPNPFDQHVVAAVASAVARAAIETGVARNKEKYEGKTLLERGNHQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHCCCCC
>Mature Secondary Structure
MENLRERALLVHRQAKGKLSVEVKVPVVDANDLSVIYSPGVAEPCKEIYANKDEMYDYTI
CCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEECCCCCHHHHHHHCCCCCCEEEEE
KGNFVAVVSNGTAVLGLGNIGARAALPVMEGKAALFKAFAGIDAVPLCLDTEDPEQIIQT
ECCEEEEEECCEEEEEECCCCCHHCCCHHCCHHHHHHHHHCCCCEEEEECCCCHHHHHHH
VKLLEPTFGGINLEDISAPACFIIEERLRQEMAIPVFHDDQHGTAIVVAAGLTNALKVVG
HHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCHHHHHHHHH
KRLADCRVVINGAGAAGVAIAKLLLSIGVGDLIVCDTKGAIYEGRPHGMNAMKEELAKQT
HHHCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCEECCCCCCHHHHHHHHHHHH
NQRRVSGMLEEVISGADVFIGVSVAGALTPAMVRSMNRGAIVFALANPVPEIMPDEAKAA
HHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHC
GAAVVATGRSDLPNQVNNVLAFPGIFRGALDVRATQINEAMKIAAAEAIAGLIQPEELTE
CEEEEECCCCCCCHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EYVIPNPFDQHVVAAVASAVARAAIETGVARNKEKYEGKTLLERGNHQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]