Click here to switch to the map view.

The map label for this gene is thiE

Identifier: 138895024

GI number: 138895024

Start: 1450236

End: 1450901

Strand: Direct

Name: thiE

Synonym: GTNG_1362

Alternate gene names: 138895024

Gene position: 1450236-1450901 (Clockwise)

Preceding gene: 138895023

Following gene: 138895026

Centisome position: 40.85

GC content: 55.71

Gene sequence:

>666_bases
ATGGCACGCATTACGAGCGAAGAGATGAAAGAACGGCTTGCTGTTTACTTTATTATGGGAAGCCAAAACAGCGAACGGCC
GGCAGAGGATGTGCTCAAAGAAGCGCTTGACGGCGGTGTGACGCTGTTTCAATTTCGCGAAAAAGGAAGTGCTGCCTTAG
AAGGGGAGGAAAAAGAAGCGCTCGCCCGGCAGTTGCAGCGTCTTTGCCGCACTTACGGCGTGCCGTTTATCGTCAACGAT
GATGTTGAGCTGGCCATCGCCATTGATGCGGACGGCGTCCACGTTGGTCAAGATGACGAAGACGCACGGCGCGTGCGGGA
AAAAATTGGCGATAAAATTCTTGGTGTTTCGGCGCATAATGTGGAAGAGGCGAGAGCGGCCATCGAAGCGGGTGCCGACT
ACATCGGCGTCGGTCCGATTTATCCGACACGTTCAAAAGACGATGCGAATGAAGCGCAAGGTCCGGGAATTCTCCGCCAC
TTGCGCGAACAAGGAATCACGATCCCGATTGTTGCTATCGGTGGAATTACGGCTGATAATACGAGAGCGGTCATTGAAGC
TGGAGCAGATGGGGTGTCAGTCATTAGCGCCATCGCTTCCGCCCCGGAGCCAAAAGCGGCGGCTGCAGCGTTGGCGACGG
CGGTGCGGGAGGCCAACCTGCGCTAA

Upstream 100 bases:

>100_bases
AATCGTTCGTACAGGCGGCGATCTCTCATTCGCTCGGCATCGGCCAAGGCCACGGGCCGACCAACCATTGGGCATACCGT
CAATACGGGGAGTGAGAACG

Downstream 100 bases:

>100_bases
ACGACAGTGAAACCGTCAAAAAAAGGGTGTCCTCAATGGGACACCCTTTTTTCGTGCTACGATGTTGTTTGCCTCCATTT
CGGCAGCCGCCATTCCAAAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MARITSEEMKERLAVYFIMGSQNSERPAEDVLKEALDGGVTLFQFREKGSAALEGEEKEALARQLQRLCRTYGVPFIVND
DVELAIAIDADGVHVGQDDEDARRVREKIGDKILGVSAHNVEEARAAIEAGADYIGVGPIYPTRSKDDANEAQGPGILRH
LREQGITIPIVAIGGITADNTRAVIEAGADGVSVISAIASAPEPKAAAAALATAVREANLR

Sequences:

>Translated_221_residues
MARITSEEMKERLAVYFIMGSQNSERPAEDVLKEALDGGVTLFQFREKGSAALEGEEKEALARQLQRLCRTYGVPFIVND
DVELAIAIDADGVHVGQDDEDARRVREKIGDKILGVSAHNVEEARAAIEAGADYIGVGPIYPTRSKDDANEAQGPGILRH
LREQGITIPIVAIGGITADNTRAVIEAGADGVSVISAIASAPEPKAAAAALATAVREANLR
>Mature_220_residues
ARITSEEMKERLAVYFIMGSQNSERPAEDVLKEALDGGVTLFQFREKGSAALEGEEKEALARQLQRLCRTYGVPFIVNDD
VELAIAIDADGVHVGQDDEDARRVREKIGDKILGVSAHNVEEARAAIEAGADYIGVGPIYPTRSKDDANEAQGPGILRHL
REQGITIPIVAIGGITADNTRAVIEAGADGVSVISAIASAPEPKAAAAALATAVREANLR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=181, Percent_Identity=35.3591160220994, Blast_Score=80, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=194, Percent_Identity=39.1752577319588, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_GEOTN (A4IN28)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001125477.1
- ProteinModelPortal:   A4IN28
- SMR:   A4IN28
- STRING:   A4IN28
- GeneID:   4966301
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_1362
- NMPDR:   fig|420246.5.peg.1321
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- PhylomeDB:   A4IN28
- ProtClustDB:   PRK00043
- BioCyc:   GTHE420246:GTNG_1362-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23486; Mature: 23354

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARITSEEMKERLAVYFIMGSQNSERPAEDVLKEALDGGVTLFQFREKGSAALEGEEKEA
CCCCCHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHH
LARQLQRLCRTYGVPFIVNDDVELAIAIDADGVHVGQDDEDARRVREKIGDKILGVSAHN
HHHHHHHHHHHCCCCEEECCCCEEEEEEECCCEECCCCCHHHHHHHHHHCCCEECCCCCC
VEEARAAIEAGADYIGVGPIYPTRSKDDANEAQGPGILRHLREQGITIPIVAIGGITADN
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCC
TRAVIEAGADGVSVISAIASAPEPKAAAAALATAVREANLR
CCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARITSEEMKERLAVYFIMGSQNSERPAEDVLKEALDGGVTLFQFREKGSAALEGEEKEA
CCCCHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHH
LARQLQRLCRTYGVPFIVNDDVELAIAIDADGVHVGQDDEDARRVREKIGDKILGVSAHN
HHHHHHHHHHHCCCCEEECCCCEEEEEEECCCEECCCCCHHHHHHHHHHCCCEECCCCCC
VEEARAAIEAGADYIGVGPIYPTRSKDDANEAQGPGILRHLREQGITIPIVAIGGITADN
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCC
TRAVIEAGADGVSVISAIASAPEPKAAAAALATAVREANLR
CCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA