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The map label for this gene is gltD [H]

Identifier: 138894955

GI number: 138894955

Start: 1379017

End: 1380498

Strand: Direct

Name: gltD [H]

Synonym: GTNG_1293

Alternate gene names: 138894955

Gene position: 1379017-1380498 (Clockwise)

Preceding gene: 138894954

Following gene: 138894956

Centisome position: 38.84

GC content: 52.09

Gene sequence:

>1482_bases
GTGGGAAAAACAACAGGGTTTATGGAATACGCTCGTGAGGAAGAGAAAAAGCGCGATCCGCTTTCCCGTCTTGAAGATTG
GAAAGAGTATGCACAACCGTTTTCCGAGGATGTACTAGCGCGCCAAGGCGCTCGCTGTATGGATTGCGGTACGCCGTTTT
GTCATATGGGGTTGGAACTCAACGGCCTCACCTCCGGGTGCCCAGTGCACAACTTAATTCCGGAATGGAACGATTTAGTA
TACCGCGGCCGTTGGAAAGAAGCGCTTGACCGGCTTTTGAAAACGAACAACTTCCCGGAATTTACGGGGCGCGTCTGTCC
GGCACCGTGTGAAGGATCGTGTACGGTTGCCATCTCCGATCCGGCGGTCGCTATTAAAGGAATTGAACGAGCCATTATTG
ATAAAGGGTTTGCAGAAGGATGGGTGAAACCTCGCATCCCAAAAACGCGCACTGGAAAAAAGGTAGCCATTGTCGGCTCC
GGTCCGGCCGGTCTCGCCTGTGCCGACCAGCTCAACCAAGCCGGCCACTCGGTGACGGTGTACGAGCGGGCGGATCGTGT
CGGCGGCTTGCTGATGTATGGCATTCCGAACATGAAATTGGAAAAAGAAGTGGTCGAGCGGCGGGTGCGCTTGCTTGAAG
AGGAAGGTGTTACATTTGTTGTGAACACCGAGGTTGGCAAAGATATCACCGCCGATGAGTTGCGCGCTGAATATGATGCG
GTTGTGCTGTGTGTCGGGGCACAAAAGCAGCGTGACTTGGTCATTGAAGGCCGAGAGCTGGAAGGCGTTCATTTTGCTAT
GGATTATTTAACCGGTGTGACGAAAAGCTTAATGGATTCGAACTTTGCCGATGGTCAGTTTATCGATGCGAAAGATAAAC
ATGTTATCGTGATTGGCGGTGGTGATACGGGGGCTGACTGTGTAGCGACCGCGCTGCGCCAAGGCTGCAAAAGCGTTGTC
CAGTTTGGCAAACATCCAGCCCTGCCAGACAAACGCGCAGACCATAACCCGTGGCCGCAATACCCGCTCGTCTTTACGCT
TGACTACGCATATGAAGAAGCGAAAGCGAAGTTCGGTGTGGATCCACGGCAATATTGCATTCAGACGAAAAAAATCGTCG
GCGATGAATATGGACGGGTAAAAGAACTGCATACGATTCAAATGGAAAAAATCATCGACGAAAACGGCAAAGCCATCTTT
AAAGAAATTCCGGGGACAGAACAAGTATGGCCGTGTGATCTTGTTTTCATCGCTATCGGTTTCGAAGGTCCGGAACAGCC
GGTGTTGAAGCAGTTTGGCGTGGAAACGGCCAACAATAAGGTAAAAGCCCCGTACGGAAAATATACGACAAACATCGAAG
GGGTGTTTGCGGCCGGCGATGCTCGCCGCGGCCAAAGCTTGATCGTTTGGGCGATTCACGAAGGCCGGGGGGCGGCTCGT
GAAGTTGATCGCTTTTTGATGGGAGAGACGAAATTGCCGTGA

Upstream 100 bases:

>100_bases
CCGCGGCCCGCGCGGGGCTCGCTTTTTGACTGGCCAAACAGGCGGGGAAGGTCGCATGAGAGAAGAAAGGCCGTATACGG
AGAGAAGGAGGGGAACCATC

Downstream 100 bases:

>100_bases
CCATAGCTTCCTCCTTAAAAAGTACTGCTTTTTAAGGAGGATTTTTTGTTTTTCCGGAAAAATATTTATAGGAAAAACAA
ACAAGGAGTGAACCGAAGTG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MGKTTGFMEYAREEEKKRDPLSRLEDWKEYAQPFSEDVLARQGARCMDCGTPFCHMGLELNGLTSGCPVHNLIPEWNDLV
YRGRWKEALDRLLKTNNFPEFTGRVCPAPCEGSCTVAISDPAVAIKGIERAIIDKGFAEGWVKPRIPKTRTGKKVAIVGS
GPAGLACADQLNQAGHSVTVYERADRVGGLLMYGIPNMKLEKEVVERRVRLLEEEGVTFVVNTEVGKDITADELRAEYDA
VVLCVGAQKQRDLVIEGRELEGVHFAMDYLTGVTKSLMDSNFADGQFIDAKDKHVIVIGGGDTGADCVATALRQGCKSVV
QFGKHPALPDKRADHNPWPQYPLVFTLDYAYEEAKAKFGVDPRQYCIQTKKIVGDEYGRVKELHTIQMEKIIDENGKAIF
KEIPGTEQVWPCDLVFIAIGFEGPEQPVLKQFGVETANNKVKAPYGKYTTNIEGVFAAGDARRGQSLIVWAIHEGRGAAR
EVDRFLMGETKLP

Sequences:

>Translated_493_residues
MGKTTGFMEYAREEEKKRDPLSRLEDWKEYAQPFSEDVLARQGARCMDCGTPFCHMGLELNGLTSGCPVHNLIPEWNDLV
YRGRWKEALDRLLKTNNFPEFTGRVCPAPCEGSCTVAISDPAVAIKGIERAIIDKGFAEGWVKPRIPKTRTGKKVAIVGS
GPAGLACADQLNQAGHSVTVYERADRVGGLLMYGIPNMKLEKEVVERRVRLLEEEGVTFVVNTEVGKDITADELRAEYDA
VVLCVGAQKQRDLVIEGRELEGVHFAMDYLTGVTKSLMDSNFADGQFIDAKDKHVIVIGGGDTGADCVATALRQGCKSVV
QFGKHPALPDKRADHNPWPQYPLVFTLDYAYEEAKAKFGVDPRQYCIQTKKIVGDEYGRVKELHTIQMEKIIDENGKAIF
KEIPGTEQVWPCDLVFIAIGFEGPEQPVLKQFGVETANNKVKAPYGKYTTNIEGVFAAGDARRGQSLIVWAIHEGRGAAR
EVDRFLMGETKLP
>Mature_492_residues
GKTTGFMEYAREEEKKRDPLSRLEDWKEYAQPFSEDVLARQGARCMDCGTPFCHMGLELNGLTSGCPVHNLIPEWNDLVY
RGRWKEALDRLLKTNNFPEFTGRVCPAPCEGSCTVAISDPAVAIKGIERAIIDKGFAEGWVKPRIPKTRTGKKVAIVGSG
PAGLACADQLNQAGHSVTVYERADRVGGLLMYGIPNMKLEKEVVERRVRLLEEEGVTFVVNTEVGKDITADELRAEYDAV
VLCVGAQKQRDLVIEGRELEGVHFAMDYLTGVTKSLMDSNFADGQFIDAKDKHVIVIGGGDTGADCVATALRQGCKSVVQ
FGKHPALPDKRADHNPWPQYPLVFTLDYAYEEAKAKFGVDPRQYCIQTKKIVGDEYGRVKELHTIQMEKIIDENGKAIFK
EIPGTEQVWPCDLVFIAIGFEGPEQPVLKQFGVETANNKVKAPYGKYTTNIEGVFAAGDARRGQSLIVWAIHEGRGAARE
VDRFLMGETKLP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=299, Percent_Identity=25.4180602006689, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI111118981, Length=119, Percent_Identity=36.9747899159664, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI111118983, Length=119, Percent_Identity=36.9747899159664, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789606, Length=490, Percent_Identity=37.9591836734694, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1788811, Length=488, Percent_Identity=35.4508196721311, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI87082180, Length=427, Percent_Identity=34.192037470726, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1788468, Length=464, Percent_Identity=26.2931034482759, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789243, Length=428, Percent_Identity=25, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17570289, Length=498, Percent_Identity=48.995983935743, Blast_Score=488, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71984108, Length=478, Percent_Identity=24.4769874476987, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17543860, Length=316, Percent_Identity=26.5822784810127, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=496, Percent_Identity=50.6048387096774, Blast_Score=511, Evalue=1e-146,
Organism=Drosophila melanogaster, GI28574881, Length=490, Percent_Identity=54.4897959183673, Blast_Score=542, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24665539, Length=490, Percent_Identity=54.4897959183673, Blast_Score=542, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24665547, Length=490, Percent_Identity=54.4897959183673, Blast_Score=540, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24665543, Length=490, Percent_Identity=54.4897959183673, Blast_Score=540, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24640763, Length=301, Percent_Identity=27.2425249169435, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI18858217, Length=301, Percent_Identity=27.2425249169435, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54604; Mature: 54473

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTTGFMEYAREEEKKRDPLSRLEDWKEYAQPFSEDVLARQGARCMDCGTPFCHMGLEL
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCEECCCCCHHHCCEEE
NGLTSGCPVHNLIPEWNDLVYRGRWKEALDRLLKTNNFPEFTGRVCPAPCEGSCTVAISD
CCCCCCCCHHHCCCCHHHHEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECC
PAVAIKGIERAIIDKGFAEGWVKPRIPKTRTGKKVAIVGSGPAGLACADQLNQAGHSVTV
CCHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEE
YERADRVGGLLMYGIPNMKLEKEVVERRVRLLEEEGVTFVVNTEVGKDITADELRAEYDA
EEHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHCCCCE
VVLCVGAQKQRDLVIEGRELEGVHFAMDYLTGVTKSLMDSNFADGQFIDAKDKHVIVIGG
EEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEEEEC
GDTGADCVATALRQGCKSVVQFGKHPALPDKRADHNPWPQYPLVFTLDYAYEEAKAKFGV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCC
DPRQYCIQTKKIVGDEYGRVKELHTIQMEKIIDENGKAIFKEIPGTEQVWPCDLVFIAIG
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEEEEEEEE
FEGPEQPVLKQFGVETANNKVKAPYGKYTTNIEGVFAAGDARRGQSLIVWAIHEGRGAAR
CCCCCHHHHHHHCCCCCCCCEECCCCCCCCCCCEEEECCCCCCCCEEEEEEEECCCCHHH
EVDRFLMGETKLP
HHHHHHHCCCCCC
>Mature Secondary Structure 
GKTTGFMEYAREEEKKRDPLSRLEDWKEYAQPFSEDVLARQGARCMDCGTPFCHMGLEL
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCEECCCCCHHHCCEEE
NGLTSGCPVHNLIPEWNDLVYRGRWKEALDRLLKTNNFPEFTGRVCPAPCEGSCTVAISD
CCCCCCCCHHHCCCCHHHHEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECC
PAVAIKGIERAIIDKGFAEGWVKPRIPKTRTGKKVAIVGSGPAGLACADQLNQAGHSVTV
CCHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEE
YERADRVGGLLMYGIPNMKLEKEVVERRVRLLEEEGVTFVVNTEVGKDITADELRAEYDA
EEHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHCCCCE
VVLCVGAQKQRDLVIEGRELEGVHFAMDYLTGVTKSLMDSNFADGQFIDAKDKHVIVIGG
EEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEEEEC
GDTGADCVATALRQGCKSVVQFGKHPALPDKRADHNPWPQYPLVFTLDYAYEEAKAKFGV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCC
DPRQYCIQTKKIVGDEYGRVKELHTIQMEKIIDENGKAIFKEIPGTEQVWPCDLVFIAIG
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEEEEEEEE
FEGPEQPVLKQFGVETANNKVKAPYGKYTTNIEGVFAAGDARRGQSLIVWAIHEGRGAAR
CCCCCHHHHHHHCCCCCCCCEECCCCCCCCCCCEEEECCCCCCCCEEEEEEEECCCCHHH
EVDRFLMGETKLP
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]