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The map label for this gene is cysL [H]

Identifier: 138894930

GI number: 138894930

Start: 1337358

End: 1338254

Strand: Reverse

Name: cysL [H]

Synonym: GTNG_1268

Alternate gene names: 138894930

Gene position: 1338254-1337358 (Counterclockwise)

Preceding gene: 138894945

Following gene: 138894925

Centisome position: 37.69

GC content: 45.71

Gene sequence:

>897_bases
ATGTACTATGACGAATTAAAAACGTTTATTACGTTGGCAGAAGTGAAAAATTTCACAAAAACAGCTGAAATCCTTCATCT
CTCCCAGCCAAGTGTCAGTTTGCACATCAAAAATTTAGAAAAAGAGTTTCAAACAAAACTGTTCATCCGTTCCCCGAAAC
GGCTGCGCATGACGCCAACCGGCGAACTTCTGTATGACCGCGCCAAACAAATGATGGCACTCTACGAACAGACGAAACAA
GACATTTTAGAGCATCACCACTCGGTGAAAGGCGAATTAAAAATCGGGGCCAGCTTTACGATCGGGGAGTATATTTTGCC
GCTGTTTTTGCTCGACTTCCAACAGCGCTATCCGGAACTTGAACTTGAAGTCATGATCGGCAACACAAAAGAAATCGTCG
AGCTCGTCCGCTCATACCAAGTGGATATCGGTTTGATCGAAGGACAAACGAATGACAAAGAACTGTCCGTTCACCCGTTC
ATGCAGGACGAACTTGTCATCGTTGCTTCAAACTGCCACGAATTGGCACAAAAAGAGGAAGTGACGATCACCGATTTGCA
AAACGAAACATGGGTAGCACGCGAAATCGGTTCCGGCACACGCGAATATTTCAATCACTTTATCCGTTCAAACGGGCTAA
AGGTGAAGTCGCTGATGATCATCAGCAGCAACCAAGGCATTAAAGAAACGTTAATCAACGGCAATGCCTTGTCCCTCCTG
TCACGCAGCGTCGTCGCCCGCGACATCGAGCACGGTCACCTGTCGATCATCCGTCTAAAAAACCCACCGTTTTACCGGAT
GTTTTCCTATATTTATTCGCCGATCATGGAAAACAAGCAAAACGTTCGCATTTTCCTTGAAACGTTGCAAACCAACTGGA
CTGGCGCCAACAAATAA

Upstream 100 bases:

>100_bases
AACAAAACATTAGTAAAATAAATATAAATTATTAAGACTATTAATATTTCTAATCAACTTCTACACCTAGATGGTGCCAT
CGCGGGCAGGGGGAATGAGT

Downstream 100 bases:

>100_bases
CAGAAGGGCGTGGTCATTGATGACCCGCCCTTCACACTTCCATCTCCCCATGGCGGATGTGTGGCATTTCGATTTGAATG
GTTGCATGGCGAATGTGGAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MYYDELKTFITLAEVKNFTKTAEILHLSQPSVSLHIKNLEKEFQTKLFIRSPKRLRMTPTGELLYDRAKQMMALYEQTKQ
DILEHHHSVKGELKIGASFTIGEYILPLFLLDFQQRYPELELEVMIGNTKEIVELVRSYQVDIGLIEGQTNDKELSVHPF
MQDELVIVASNCHELAQKEEVTITDLQNETWVAREIGSGTREYFNHFIRSNGLKVKSLMIISSNQGIKETLINGNALSLL
SRSVVARDIEHGHLSIIRLKNPPFYRMFSYIYSPIMENKQNVRIFLETLQTNWTGANK

Sequences:

>Translated_298_residues
MYYDELKTFITLAEVKNFTKTAEILHLSQPSVSLHIKNLEKEFQTKLFIRSPKRLRMTPTGELLYDRAKQMMALYEQTKQ
DILEHHHSVKGELKIGASFTIGEYILPLFLLDFQQRYPELELEVMIGNTKEIVELVRSYQVDIGLIEGQTNDKELSVHPF
MQDELVIVASNCHELAQKEEVTITDLQNETWVAREIGSGTREYFNHFIRSNGLKVKSLMIISSNQGIKETLINGNALSLL
SRSVVARDIEHGHLSIIRLKNPPFYRMFSYIYSPIMENKQNVRIFLETLQTNWTGANK
>Mature_298_residues
MYYDELKTFITLAEVKNFTKTAEILHLSQPSVSLHIKNLEKEFQTKLFIRSPKRLRMTPTGELLYDRAKQMMALYEQTKQ
DILEHHHSVKGELKIGASFTIGEYILPLFLLDFQQRYPELELEVMIGNTKEIVELVRSYQVDIGLIEGQTNDKELSVHPF
MQDELVIVASNCHELAQKEEVTITDLQNETWVAREIGSGTREYFNHFIRSNGLKVKSLMIISSNQGIKETLINGNALSLL
SRSVVARDIEHGHLSIIRLKNPPFYRMFSYIYSPIMENKQNVRIFLETLQTNWTGANK

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=283, Percent_Identity=33.2155477031802, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI157672245, Length=258, Percent_Identity=28.2945736434109, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790399, Length=295, Percent_Identity=22.0338983050847, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=25.4295532646048, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI145693105, Length=196, Percent_Identity=23.9795918367347, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788748, Length=236, Percent_Identity=22.8813559322034, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787806, Length=246, Percent_Identity=21.5447154471545, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082132, Length=260, Percent_Identity=26.9230769230769, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788887, Length=271, Percent_Identity=27.6752767527675, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787530, Length=231, Percent_Identity=26.8398268398268, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788296, Length=227, Percent_Identity=25.9911894273128, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994962, Length=141, Percent_Identity=29.7872340425532, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790208, Length=168, Percent_Identity=30.3571428571429, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790262, Length=209, Percent_Identity=24.8803827751196, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788297, Length=174, Percent_Identity=24.1379310344828, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787879, Length=201, Percent_Identity=25.3731343283582, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787601, Length=138, Percent_Identity=23.9130434782609, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788706, Length=180, Percent_Identity=30.5555555555556, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789506, Length=221, Percent_Identity=22.6244343891403, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87081904, Length=220, Percent_Identity=25.9090909090909, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=227, Percent_Identity=24.6696035242291, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI145693193, Length=119, Percent_Identity=30.2521008403361, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34509; Mature: 34509

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYDELKTFITLAEVKNFTKTAEILHLSQPSVSLHIKNLEKEFQTKLFIRSPKRLRMTPT
CCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEHHHHHHHHHHHEEECCCEEEECCC
GELLYDRAKQMMALYEQTKQDILEHHHSVKGELKIGASFTIGEYILPLFLLDFQQRYPEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEHHHHHHHHHHHHHHHHCCCE
ELEVMIGNTKEIVELVRSYQVDIGLIEGQTNDKELSVHPFMQDELVIVASNCHELAQKEE
EEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCCEEECCCCCCCEEEEECCHHHHHHCCC
VTITDLQNETWVAREIGSGTREYFNHFIRSNGLKVKSLMIISSNQGIKETLINGNALSLL
EEEEECCCCCCHHHHHCCCHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHCCCCHHHHH
SRSVVARDIEHGHLSIIRLKNPPFYRMFSYIYSPIMENKQNVRIFLETLQTNWTGANK
HHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCCC
>Mature Secondary Structure
MYYDELKTFITLAEVKNFTKTAEILHLSQPSVSLHIKNLEKEFQTKLFIRSPKRLRMTPT
CCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEHHHHHHHHHHHEEECCCEEEECCC
GELLYDRAKQMMALYEQTKQDILEHHHSVKGELKIGASFTIGEYILPLFLLDFQQRYPEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEHHHHHHHHHHHHHHHHCCCE
ELEVMIGNTKEIVELVRSYQVDIGLIEGQTNDKELSVHPFMQDELVIVASNCHELAQKEE
EEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCCEEECCCCCCCEEEEECCHHHHHHCCC
VTITDLQNETWVAREIGSGTREYFNHFIRSNGLKVKSLMIISSNQGIKETLINGNALSLL
EEEEECCCCCCHHHHHCCCHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHCCCCHHHHH
SRSVVARDIEHGHLSIIRLKNPPFYRMFSYIYSPIMENKQNVRIFLETLQTNWTGANK
HHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]