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The map label for this gene is murD [H]

Identifier: 138894653

GI number: 138894653

Start: 1050001

End: 1051323

Strand: Direct

Name: murD [H]

Synonym: GTNG_0983

Alternate gene names: 138894653

Gene position: 1050001-1051323 (Clockwise)

Preceding gene: 138894652

Following gene: 138894654

Centisome position: 29.57

GC content: 52.76

Gene sequence:

>1323_bases
GTGCTTGTCATTGGATTGGCTAAAAGTGGGGCGGCGGCGGCTCGGTTGCTTGCTGAATTAGGTGTTGAAGTCGTCGCCAA
CGACCAAAAACCGTTGGAGGAAAATGCGGAAGCGAAGAAGCTGGAGCAGCTCGGTATCCGCGTCGTTTGTGGTGGTCATC
CGCTCGAGCTGCTCGATGAGCCATTTGATCTTGTTGTGAAAAATCCAGGAATTCCGTATACAAACCCGTTGGTCGAAAAG
GCGCTCGAAAAAGGGCTTCCGATTGTGACAGAAGTGGAGCTTGCCTATCGTATTTCTGAGGCGCCGTTTATCGGTATTAC
CGGGTCGAACGGAAAAACGACGACAACGACGCTCATTTATGAGATGTTAAAGGCGGACGGCCAAGACCCGCTGCTTGCTG
GCAATATTGGTCTTGTGGCCTGCGAGGTGGCAAAGGAAGCGAAACCGGATCAATGGCTTGTCACCGAGCTGTCTTCGTTC
CAACTGGCCGGCATTGACGAGTTTCGTCCGAGGATCGCGGTCTTGCTCAACATTTTTGACGCCCACTTGGATTACCACGG
GACGAAAGAAGCATATGCAGCGGCGAAGGCGAACATTTTCCGCAACCAAACAGCACACGATTACGCCGTCGTCAATGCGG
ATGATCCGCTTGTGATGGACATCGTCTCGTCCGTTCGGGCGCAAAAGGTGCTGTTTTCGGTGACAAAGCCGCTTGGTGAA
GGGGCGTACGTCCAAGATGGCACTGTTTATTGGAACGGTGAGTCGGTCATCCAGATCGCCGACATCGTTCTCCCCGGTCA
GCACAATTTGGAAAATATTTTAGCTGCAGTAGCAGCTGCTAAACTAGCTGGCGCTAGCAATGAGGCCATCCGTCGGGTGT
TGACGACGTTTACGGGCGTGAAGCACCGACTTCAATATGTGGCTGAAGTCGACGGACGGCGATTTTACAATGATTCGAAG
GCGACGAACATATTGGCGACGCAAAAGGCGCTCGCGGCGTTTGCTGATGAGCCGGTCATTTTGCTGGCCGGTGGGCTTGA
TCGCGGCAATGAATTTGATGCCCTTCTCCCATACTTGCAACAGGTCAAAGCCATGGTGCTGTTTGGCCAAACGGCGGCCA
AGATTGGCCGCGTGGCTCGGGAGGCGGGAATAGAAACGGTAGAATATGTCGATAATGTGGAAAAGGCCGTCCCGGTTGCC
TTTCGGCTTTCAGAGCCGGGCGATGTCATTTTGCTCTCTCCGGCATGTGCTAGTTGGGATCAATATAAAACTTTCGAGGA
GAGAGGGGACATTTTTATTAACGCCGTGCATAAGTTGAAATAG

Upstream 100 bases:

>100_bases
CGTCACGTTTTGGGCTGTCGGCCTTTTATTCGCAATGCTAGGAATTTATATCGAGGTGTGGATTTAATTGAAACCGACTC
CTTTTTATCAACATCGTCGT

Downstream 100 bases:

>100_bases
AGGGAAAGGGACAGCCCTTTCCCTCTAGTTTACAAAATAGAGGTGTTGGGCATTGCCGCGAAAAAAGTCTGCACCGGATT
TTTTGCTCATTATTTTAACG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLVIGLAKSGAAAARLLAELGVEVVANDQKPLEENAEAKKLEQLGIRVVCGGHPLELLDEPFDLVVKNPGIPYTNPLVEK
ALEKGLPIVTEVELAYRISEAPFIGITGSNGKTTTTTLIYEMLKADGQDPLLAGNIGLVACEVAKEAKPDQWLVTELSSF
QLAGIDEFRPRIAVLLNIFDAHLDYHGTKEAYAAAKANIFRNQTAHDYAVVNADDPLVMDIVSSVRAQKVLFSVTKPLGE
GAYVQDGTVYWNGESVIQIADIVLPGQHNLENILAAVAAAKLAGASNEAIRRVLTTFTGVKHRLQYVAEVDGRRFYNDSK
ATNILATQKALAAFADEPVILLAGGLDRGNEFDALLPYLQQVKAMVLFGQTAAKIGRVAREAGIETVEYVDNVEKAVPVA
FRLSEPGDVILLSPACASWDQYKTFEERGDIFINAVHKLK

Sequences:

>Translated_440_residues
MLVIGLAKSGAAAARLLAELGVEVVANDQKPLEENAEAKKLEQLGIRVVCGGHPLELLDEPFDLVVKNPGIPYTNPLVEK
ALEKGLPIVTEVELAYRISEAPFIGITGSNGKTTTTTLIYEMLKADGQDPLLAGNIGLVACEVAKEAKPDQWLVTELSSF
QLAGIDEFRPRIAVLLNIFDAHLDYHGTKEAYAAAKANIFRNQTAHDYAVVNADDPLVMDIVSSVRAQKVLFSVTKPLGE
GAYVQDGTVYWNGESVIQIADIVLPGQHNLENILAAVAAAKLAGASNEAIRRVLTTFTGVKHRLQYVAEVDGRRFYNDSK
ATNILATQKALAAFADEPVILLAGGLDRGNEFDALLPYLQQVKAMVLFGQTAAKIGRVAREAGIETVEYVDNVEKAVPVA
FRLSEPGDVILLSPACASWDQYKTFEERGDIFINAVHKLK
>Mature_440_residues
MLVIGLAKSGAAAARLLAELGVEVVANDQKPLEENAEAKKLEQLGIRVVCGGHPLELLDEPFDLVVKNPGIPYTNPLVEK
ALEKGLPIVTEVELAYRISEAPFIGITGSNGKTTTTTLIYEMLKADGQDPLLAGNIGLVACEVAKEAKPDQWLVTELSSF
QLAGIDEFRPRIAVLLNIFDAHLDYHGTKEAYAAAKANIFRNQTAHDYAVVNADDPLVMDIVSSVRAQKVLFSVTKPLGE
GAYVQDGTVYWNGESVIQIADIVLPGQHNLENILAAVAAAKLAGASNEAIRRVLTTFTGVKHRLQYVAEVDGRRFYNDSK
ATNILATQKALAAFADEPVILLAGGLDRGNEFDALLPYLQQVKAMVLFGQTAAKIGRVAREAGIETVEYVDNVEKAVPVA
FRLSEPGDVILLSPACASWDQYKTFEERGDIFINAVHKLK

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=376, Percent_Identity=38.563829787234, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1786279, Length=304, Percent_Identity=28.2894736842105, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47587; Mature: 47587

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVIGLAKSGAAAARLLAELGVEVVANDQKPLEENAEAKKLEQLGIRVVCGGHPLELLDE
CEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCEEEEECCCCHHHHCC
PFDLVVKNPGIPYTNPLVEKALEKGLPIVTEVELAYRISEAPFIGITGSNGKTTTTTLIY
CCEEEEECCCCCCCCHHHHHHHHCCCCEEEEEHHHEEECCCCEEEEECCCCCCHHHHHHH
EMLKADGQDPLLAGNIGLVACEVAKEAKPDQWLVTELSSFQLAGIDEFRPRIAVLLNIFD
HHHHCCCCCCEEECCCCEEEEEHHHHCCCCCHHHHHHCCCEECCCHHHHHHHHHHHHHHH
AHLDYHGTKEAYAAAKANIFRNQTAHDYAVVNADDPLVMDIVSSVRAQKVLFSVTKPLGE
HHCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
GAYVQDGTVYWNGESVIQIADIVLPGQHNLENILAAVAAAKLAGASNEAIRRVLTTFTGV
CCEEECCEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KHRLQYVAEVDGRRFYNDSKATNILATQKALAAFADEPVILLAGGLDRGNEFDALLPYLQ
HHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
QVKAMVLFGQTAAKIGRVAREAGIETVEYVDNVEKAVPVAFRLSEPGDVILLSPACASWD
HHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCCCH
QYKTFEERGDIFINAVHKLK
HHHHHHHCCCEEEEEHHHCC
>Mature Secondary Structure
MLVIGLAKSGAAAARLLAELGVEVVANDQKPLEENAEAKKLEQLGIRVVCGGHPLELLDE
CEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCEEEEECCCCHHHHCC
PFDLVVKNPGIPYTNPLVEKALEKGLPIVTEVELAYRISEAPFIGITGSNGKTTTTTLIY
CCEEEEECCCCCCCCHHHHHHHHCCCCEEEEEHHHEEECCCCEEEEECCCCCCHHHHHHH
EMLKADGQDPLLAGNIGLVACEVAKEAKPDQWLVTELSSFQLAGIDEFRPRIAVLLNIFD
HHHHCCCCCCEEECCCCEEEEEHHHHCCCCCHHHHHHCCCEECCCHHHHHHHHHHHHHHH
AHLDYHGTKEAYAAAKANIFRNQTAHDYAVVNADDPLVMDIVSSVRAQKVLFSVTKPLGE
HHCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
GAYVQDGTVYWNGESVIQIADIVLPGQHNLENILAAVAAAKLAGASNEAIRRVLTTFTGV
CCEEECCEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KHRLQYVAEVDGRRFYNDSKATNILATQKALAAFADEPVILLAGGLDRGNEFDALLPYLQ
HHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
QVKAMVLFGQTAAKIGRVAREAGIETVEYVDNVEKAVPVAFRLSEPGDVILLSPACASWD
HHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCCCH
QYKTFEERGDIFINAVHKLK
HHHHHHHCCCEEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA