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The map label for this gene is murE

Identifier: 138894650

GI number: 138894650

Start: 1047075

End: 1048544

Strand: Direct

Name: murE

Synonym: GTNG_0980

Alternate gene names: 138894650

Gene position: 1047075-1048544 (Clockwise)

Preceding gene: 138894649

Following gene: 138894651

Centisome position: 29.49

GC content: 54.49

Gene sequence:

>1470_bases
ATGAAATTACAAACGCTGCTGTCACGCTTGCCCGGCTTTTGGGTGCATCGCGGGGGCAACCCGGATATCGTTGCGCTGGA
GATGGACTCGCGCCATGTCACGCCTGGTTCTCTCTTTTTTTGTGTGAAAGGATTTACCGTGGATGGACACGATTTTGCCG
AACAGGCGGTGGAGCGCGGGGCGGTGGCGATTGTCGCCGAGCGCCCGCTCTCGGTGAATGTGCCGGTTGTTGTTGTGCCG
GACAGCCGACGAGCGATGGCGATTTTAGCGGATGCGTTTTACGGTCAGCCGACTCACCGCCTTCATTTAATCGGAGTGAC
GGGCACAAACGGTAAAACAACGACGACTCATATCATTGAGCAGGTGGCGAGAAAAGCCGGCAAGAAAATCGGACTCATCG
GCACGGTTGGGACGAAAATCGGCGACCGTTCTTACCCGGCAGTGAATACGACACCGGAGTCACTTGTCTTGCAGCGTACG
TTTAAACAAATGGTTGACGAAGGTGTGGAGTTTGTAACCATGGAAGTGTCATCACACGCCCTTCACCAAGGACGGGTGCA
CGGTTGCGATTACGACGTCGCCGTGTTTACGAACTTGACACAAGACCACTTAGACTATCATGGAACAATGGAAGAATATC
GGAACGCCAAAGGGCTGCTGTTTGCCCAACTTGGCAACCGTTACGACGAGCGGCGGCCAAAATTTGCCGTTCTCAATCAT
GACGACCCCGTTTCGCAATATTATAAACATATGACAGCTGCACCGATCATCACATATGGCATAAAGGAAAAAAGTGATGT
GATGGCAGAGCAAATCGAAATGACTCCCGGCGGCATGGCATTTCAACTGTGCACTCCGCATGGGACCATGGCGATCGAAA
CGAAGCTGGTCGGCCTGTTTAACGTCTATAACCTCCTCGCTGCCACAGCCGCTTGCCTTGCGTCCGGCTTTTCGCTTGCG
ACGATTGCCGAGGCGCTCGCTAATGTCTCGCCGGTGCCGGGGCGGTTTGAAACGGTCGATGAAGGGCAAAATTTTACTGT
TATTGTCGATTATGCTCATACGCCGGATAGTGTGGAAAATGCACTGAAAACCGTGCGGCAATTTGCAAAGCGAAACGTGT
ATGTTGTGATCGGCTGCGGCGGTGACCGCGACCGAACAAAGCGGCCGCTCATGGCGCAAGCGGCGGTGCGCTATGCGGAT
GTCGCTGTTTTTACCTCCGACAATCCGCGCTCGGAAGACCCGAGGCAAATTTTGCGCGATATGGAGGCCGGCGTTAGTGC
TGGGGACGGGACGTATGTGACCATTCCCGACCGGGAAGAGGCGATCCGGTATGCCATTGGGCAGGCGCAAGAAGGTGATG
TCGTGTTAATTGCCGGCAAAGGGCATGAAACGTATCAAATCATCGGCGACGATGTGATCGACTTTGACGATCGCGCTGTC
GCCCGAGCGGCGGTGAAGGAGAGAAGATGA

Upstream 100 bases:

>100_bases
GGGGCGGCCGATGCGATGATTCATCGGGTTGGTTGTGCCCCTTTTTCCGCTGTCCGCATCGTTGGGCGGGCTATTTATAA
GCGAAAGGGATGAAGCAAAC

Downstream 100 bases:

>100_bases
CGATGGTTACGTATAAAATGGTGCAATCCATCGCGGTGGAGGCGCGCGGCATTATCGATGAGACGATTCGTTTTCACGCC
GTGTTTGTCGACCCCGCGAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MKLQTLLSRLPGFWVHRGGNPDIVALEMDSRHVTPGSLFFCVKGFTVDGHDFAEQAVERGAVAIVAERPLSVNVPVVVVP
DSRRAMAILADAFYGQPTHRLHLIGVTGTNGKTTTTHIIEQVARKAGKKIGLIGTVGTKIGDRSYPAVNTTPESLVLQRT
FKQMVDEGVEFVTMEVSSHALHQGRVHGCDYDVAVFTNLTQDHLDYHGTMEEYRNAKGLLFAQLGNRYDERRPKFAVLNH
DDPVSQYYKHMTAAPIITYGIKEKSDVMAEQIEMTPGGMAFQLCTPHGTMAIETKLVGLFNVYNLLAATAACLASGFSLA
TIAEALANVSPVPGRFETVDEGQNFTVIVDYAHTPDSVENALKTVRQFAKRNVYVVIGCGGDRDRTKRPLMAQAAVRYAD
VAVFTSDNPRSEDPRQILRDMEAGVSAGDGTYVTIPDREEAIRYAIGQAQEGDVVLIAGKGHETYQIIGDDVIDFDDRAV
ARAAVKERR

Sequences:

>Translated_489_residues
MKLQTLLSRLPGFWVHRGGNPDIVALEMDSRHVTPGSLFFCVKGFTVDGHDFAEQAVERGAVAIVAERPLSVNVPVVVVP
DSRRAMAILADAFYGQPTHRLHLIGVTGTNGKTTTTHIIEQVARKAGKKIGLIGTVGTKIGDRSYPAVNTTPESLVLQRT
FKQMVDEGVEFVTMEVSSHALHQGRVHGCDYDVAVFTNLTQDHLDYHGTMEEYRNAKGLLFAQLGNRYDERRPKFAVLNH
DDPVSQYYKHMTAAPIITYGIKEKSDVMAEQIEMTPGGMAFQLCTPHGTMAIETKLVGLFNVYNLLAATAACLASGFSLA
TIAEALANVSPVPGRFETVDEGQNFTVIVDYAHTPDSVENALKTVRQFAKRNVYVVIGCGGDRDRTKRPLMAQAAVRYAD
VAVFTSDNPRSEDPRQILRDMEAGVSAGDGTYVTIPDREEAIRYAIGQAQEGDVVLIAGKGHETYQIIGDDVIDFDDRAV
ARAAVKERR
>Mature_489_residues
MKLQTLLSRLPGFWVHRGGNPDIVALEMDSRHVTPGSLFFCVKGFTVDGHDFAEQAVERGAVAIVAERPLSVNVPVVVVP
DSRRAMAILADAFYGQPTHRLHLIGVTGTNGKTTTTHIIEQVARKAGKKIGLIGTVGTKIGDRSYPAVNTTPESLVLQRT
FKQMVDEGVEFVTMEVSSHALHQGRVHGCDYDVAVFTNLTQDHLDYHGTMEEYRNAKGLLFAQLGNRYDERRPKFAVLNH
DDPVSQYYKHMTAAPIITYGIKEKSDVMAEQIEMTPGGMAFQLCTPHGTMAIETKLVGLFNVYNLLAATAACLASGFSLA
TIAEALANVSPVPGRFETVDEGQNFTVIVDYAHTPDSVENALKTVRQFAKRNVYVVIGCGGDRDRTKRPLMAQAAVRYAD
VAVFTSDNPRSEDPRQILRDMEAGVSAGDGTYVTIPDREEAIRYAIGQAQEGDVVLIAGKGHETYQIIGDDVIDFDDRAV
ARAAVKERR

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=487, Percent_Identity=39.4250513347023, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1786274, Length=374, Percent_Identity=26.7379679144385, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_GEOTN (A4IM04)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001125103.1
- ProteinModelPortal:   A4IM04
- SMR:   A4IM04
- STRING:   A4IM04
- GeneID:   4965923
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_0980
- NMPDR:   fig|420246.5.peg.947
- eggNOG:   COG0769
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- PhylomeDB:   A4IM04
- ProtClustDB:   PRK00139
- BioCyc:   GTHE420246:GTNG_0980-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 53536; Mature: 53536

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 149-149 BINDING 177-177 BINDING 183-183 BINDING 185-185 BINDING 383-383 BINDING 459-459 BINDING 463-463

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQTLLSRLPGFWVHRGGNPDIVALEMDSRHVTPGSLFFCVKGFTVDGHDFAEQAVERG
CCHHHHHHHCCCEEEECCCCCCEEEEEECCCCCCCCEEEEEEECCEECCHHHHHHHHHCC
AVAIVAERPLSVNVPVVVVPDSRRAMAILADAFYGQPTHRLHLIGVTGTNGKTTTTHIIE
CEEEEECCCCEECCCEEEECCCCCCHHEEHHHHCCCCCCEEEEEEEECCCCCCHHHHHHH
QVARKAGKKIGLIGTVGTKIGDRSYPAVNTTPESLVLQRTFKQMVDEGVEFVTMEVSSHA
HHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHH
LHQGRVHGCDYDVAVFTNLTQDHLDYHGTMEEYRNAKGLLFAQLGNRYDERRPKFAVLNH
HHCCCCCCCCCCCCEEECCCHHHHHCCCCHHHHCCCCCEEEEHHCCHHHHCCCCEEEECC
DDPVSQYYKHMTAAPIITYGIKEKSDVMAEQIEMTPGGMAFQLCTPHGTMAIETKLVGLF
CCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEHHHHHHH
NVYNLLAATAACLASGFSLATIAEALANVSPVPGRFETVDEGQNFTVIVDYAHTPDSVEN
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHH
ALKTVRQFAKRNVYVVIGCGGDRDRTKRPLMAQAAVRYADVAVFTSDNPRSEDPRQILRD
HHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCHHHHHHH
MEAGVSAGDGTYVTIPDREEAIRYAIGQAQEGDVVLIAGKGHETYQIIGDDVIDFDDRAV
HHCCCCCCCCCEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHH
ARAAVKERR
HHHHHHHCC
>Mature Secondary Structure
MKLQTLLSRLPGFWVHRGGNPDIVALEMDSRHVTPGSLFFCVKGFTVDGHDFAEQAVERG
CCHHHHHHHCCCEEEECCCCCCEEEEEECCCCCCCCEEEEEEECCEECCHHHHHHHHHCC
AVAIVAERPLSVNVPVVVVPDSRRAMAILADAFYGQPTHRLHLIGVTGTNGKTTTTHIIE
CEEEEECCCCEECCCEEEECCCCCCHHEEHHHHCCCCCCEEEEEEEECCCCCCHHHHHHH
QVARKAGKKIGLIGTVGTKIGDRSYPAVNTTPESLVLQRTFKQMVDEGVEFVTMEVSSHA
HHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHH
LHQGRVHGCDYDVAVFTNLTQDHLDYHGTMEEYRNAKGLLFAQLGNRYDERRPKFAVLNH
HHCCCCCCCCCCCCEEECCCHHHHHCCCCHHHHCCCCCEEEEHHCCHHHHCCCCEEEECC
DDPVSQYYKHMTAAPIITYGIKEKSDVMAEQIEMTPGGMAFQLCTPHGTMAIETKLVGLF
CCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEHHHHHHH
NVYNLLAATAACLASGFSLATIAEALANVSPVPGRFETVDEGQNFTVIVDYAHTPDSVEN
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHH
ALKTVRQFAKRNVYVVIGCGGDRDRTKRPLMAQAAVRYADVAVFTSDNPRSEDPRQILRD
HHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCHHHHHHH
MEAGVSAGDGTYVTIPDREEAIRYAIGQAQEGDVVLIAGKGHETYQIIGDDVIDFDDRAV
HHCCCCCCCCCEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHH
ARAAVKERR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA