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The map label for this gene is narK [H]

Identifier: 138894321

GI number: 138894321

Start: 724857

End: 726032

Strand: Direct

Name: narK [H]

Synonym: GTNG_0649

Alternate gene names: 138894321

Gene position: 724857-726032 (Clockwise)

Preceding gene: 138894320

Following gene: 138894322

Centisome position: 20.42

GC content: 51.11

Gene sequence:

>1176_bases
ATGAATAAACAAAAAGCATTGTTGCCGATTACGACATTAGCCATGACGTTTTCGTTCGCTGTTTGGGCCGTCTTTTCGCC
GCTGGCGAGCACGTTCCAAGAAATGTACGGGTTGACATCGACACAAAAAAGTATTTTAGTCGCCATTCCGGTGTTGCTTG
GTTCAATCATGCGTCTGCCGCTTGGCATTTGGACGGACCGGATTGGCGGGCGTAGGCTGTTTTCCCTGTTGTTGTTGTTT
TTAATCGTGCCGCTCATTGGCGCGGGGTTTGCGGATTCATATGCGATGTTAATGTTTTGGGCGTTTTTCATCGGCATGGC
CGGGACATCGTTCGCGATCTCGGTAACGTTCGTGTCGCGCTTGACTCCTCCGGAAAAGCAAGGGACGGCGCTGGGGATTA
ACGCGATGGGCAACTTCGGCACAGCAGTGGCCAGCTTTTCCATTCCGTCAATTGCTGCTGCTTTCGGTGTTTCGTGGGCG
TTTTGGGGAATGATCATCCCGATCGTCATCATGCTTATTCTCGTTTGGTTTTTCACACCGGATATGCCGAAACCAAAGCA
ACAAAAAACGATGCGCGAATCGCTGTCCGTGCTGAAATATAAACATACGTGGACGTTGTCGCTGTTTTACTTTGTCACAT
TCGGAGCATTTGTCGCTTTTGGTATTTACTTGCCGTCGCTCCTCGTTGACTTGTATGGGTTGACACCGGTCGATGCAGGG
ATGCGTGCCGCTGGATTTACCGTACTCGCTACGCTTGCGCGACCAGTTGGCGGCAACCTCGGCGATAAAATTGGCGCCGA
GCGGGTGTTGACGTTCGTCTACGCTGGAATGACAATCGGCGCATTAGTGATCGCGTTTTGGATGGAAAACATTTGGGTGA
TGACGGCAGCCTGCCTGTTGTTAGCGGTGATGTCTGGCTTAGGAAACGGTGCGGTCTTCAAACTCGTGCCACAGTTGTTC
CCAGCAGAAACGGGGGCTGTTACTGGTCTTGTCGGCGCTTGGGGCGGACTGGGCGGCTTTTTCCCGCCGATTTTAATGGG
GATCATCAAAGACGTCACTGGTTCTTACGTGCTCGGGTTTATGCTTCTTAGCTTATTTACGTTGATCTGCTTCTTATTGA
ATCGAATCGTCTTTGGCAAAAAAGCAGGCGAACCGGCAAATCGGTACGCATCGTAA

Upstream 100 bases:

>100_bases
TAAACATCACAAACTTTCCGCCCTCGGTCACGTATGATGAAACTACAAACGATGCCTAGCGTTTGGAAGTAGAGTCCGGG
AGGAAAAAGGAGGGCTTTTC

Downstream 100 bases:

>100_bases
CACCTTAAAGAGGTCAAAAGCATGTGCTTTTGGCCTCTTTTTCATTTGGGAAGACGACGGATTTAACGAAAGAAAGACAA
GTGACGGAGGCGGAGATGAT

Product: nitrate/nitrite extrusion protein

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MNKQKALLPITTLAMTFSFAVWAVFSPLASTFQEMYGLTSTQKSILVAIPVLLGSIMRLPLGIWTDRIGGRRLFSLLLLF
LIVPLIGAGFADSYAMLMFWAFFIGMAGTSFAISVTFVSRLTPPEKQGTALGINAMGNFGTAVASFSIPSIAAAFGVSWA
FWGMIIPIVIMLILVWFFTPDMPKPKQQKTMRESLSVLKYKHTWTLSLFYFVTFGAFVAFGIYLPSLLVDLYGLTPVDAG
MRAAGFTVLATLARPVGGNLGDKIGAERVLTFVYAGMTIGALVIAFWMENIWVMTAACLLLAVMSGLGNGAVFKLVPQLF
PAETGAVTGLVGAWGGLGGFFPPILMGIIKDVTGSYVLGFMLLSLFTLICFLLNRIVFGKKAGEPANRYAS

Sequences:

>Translated_391_residues
MNKQKALLPITTLAMTFSFAVWAVFSPLASTFQEMYGLTSTQKSILVAIPVLLGSIMRLPLGIWTDRIGGRRLFSLLLLF
LIVPLIGAGFADSYAMLMFWAFFIGMAGTSFAISVTFVSRLTPPEKQGTALGINAMGNFGTAVASFSIPSIAAAFGVSWA
FWGMIIPIVIMLILVWFFTPDMPKPKQQKTMRESLSVLKYKHTWTLSLFYFVTFGAFVAFGIYLPSLLVDLYGLTPVDAG
MRAAGFTVLATLARPVGGNLGDKIGAERVLTFVYAGMTIGALVIAFWMENIWVMTAACLLLAVMSGLGNGAVFKLVPQLF
PAETGAVTGLVGAWGGLGGFFPPILMGIIKDVTGSYVLGFMLLSLFTLICFLLNRIVFGKKAGEPANRYAS
>Mature_391_residues
MNKQKALLPITTLAMTFSFAVWAVFSPLASTFQEMYGLTSTQKSILVAIPVLLGSIMRLPLGIWTDRIGGRRLFSLLLLF
LIVPLIGAGFADSYAMLMFWAFFIGMAGTSFAISVTFVSRLTPPEKQGTALGINAMGNFGTAVASFSIPSIAAAFGVSWA
FWGMIIPIVIMLILVWFFTPDMPKPKQQKTMRESLSVLKYKHTWTLSLFYFVTFGAFVAFGIYLPSLLVDLYGLTPVDAG
MRAAGFTVLATLARPVGGNLGDKIGAERVLTFVYAGMTIGALVIAFWMENIWVMTAACLLLAVMSGLGNGAVFKLVPQLF
PAETGAVTGLVGAWGGLGGFFPPILMGIIKDVTGSYVLGFMLLSLFTLICFLLNRIVFGKKAGEPANRYAS

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=429, Percent_Identity=26.8065268065268, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787743, Length=448, Percent_Identity=25.8928571428571, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42176; Mature: 42176

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]