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The map label for this gene is dagK [H]

Identifier: 138893973

GI number: 138893973

Start: 337598

End: 338518

Strand: Direct

Name: dagK [H]

Synonym: GTNG_0297

Alternate gene names: 138893973

Gene position: 337598-338518 (Clockwise)

Preceding gene: 138893972

Following gene: 138893974

Centisome position: 9.51

GC content: 52.55

Gene sequence:

>921_bases
ATGAAACGAGCTCGAGTGATTTATAATCCGACATCGGGACGGGAGCTGTTTAAGCGCCATTTGCCCGATGTGCTCGTCCG
ATTAGAAAAGGCTGGATATGAAACGTCGTGCCACGCTACTGAAGGGCCGGGGGATGCGACGAAAGCAGCGAGGCAAGCGG
CCGAGAGGGAATTTGACCTCGTTGTGGCGGCAGGCGGCGATGGAACGATTAACGAGGTGGTCAATGGCATTGCGAATCAG
CCATACCGCCCGAAGCTCGGCGTGATTCCTGTTGGAACGACGAACGATTTTGCCCGTGCCATCGGTGTGCCGCGTTCGAT
CGAAGGGGCGTGCGAGGTGATCGCTACGGGTGAACCTGTCGCGATTGACATCGGCTCTGTCACCAATGAAGATCAGACAC
GTTATTTCATCAATATCGCGGGCGGCGGGCGCCTGACCGAGCTCACCTATGAAGTGCCAAGCAAGTTGAAAACGATGCTC
GGCCAGCTGGCCTATTACTTAAAAGGGATTGAAATGCTTCCGTCGATTAAGGCAACGGAGGCGCAAATTGAGTATGACGG
CAAACTATTTGAAGGCGAAATCATGATGTTTTTAGTCTCGCTGACCAACTCGGTCGGCGGCTTTGAAAAATTAGCGCCTG
ATTCGTCACTCAATGACGGCCTGTTTGACTTCATCATTGTCAAGAAAACGAATTTGGCTGAGTTTATCCGTCTCGTCACG
CTAGCGGCGCGCGGGGAGCACATTAACGACCCGCATGTCATTTACACGAAAGCCAATCGGGTGAAAGTACGCTCTTCGAT
GCAGCTGAACTTAGACGGCGAATTCGGCGGCATGCTTCCGGGCGAGTTTGTCAACTTATACCGCCATCTCGAAGTATTCA
TGCCAAAAGAAAAAGCAGCACAAGTGAGAACAGGGCCATAA

Upstream 100 bases:

>100_bases
TTTGCTTCATAGTTGCGCAGGGAGCGCCGTTTCGCTATGATGTGAAATAGTGGAAGGTTCCTTTTCAAGGATGGAAAAAG
AGGAAGTAGGTTGATGATGA

Downstream 100 bases:

>100_bases
GCCTGTTCTTTTCTTATGTCCTCACGCTAGAAGGGGCACGCTAAACATACACGATGGTCAGAAAGGGGAACGACATGGCG
AAACAACAAGCACCGGTCGC

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKRARVIYNPTSGRELFKRHLPDVLVRLEKAGYETSCHATEGPGDATKAARQAAEREFDLVVAAGGDGTINEVVNGIANQ
PYRPKLGVIPVGTTNDFARAIGVPRSIEGACEVIATGEPVAIDIGSVTNEDQTRYFINIAGGGRLTELTYEVPSKLKTML
GQLAYYLKGIEMLPSIKATEAQIEYDGKLFEGEIMMFLVSLTNSVGGFEKLAPDSSLNDGLFDFIIVKKTNLAEFIRLVT
LAARGEHINDPHVIYTKANRVKVRSSMQLNLDGEFGGMLPGEFVNLYRHLEVFMPKEKAAQVRTGP

Sequences:

>Translated_306_residues
MKRARVIYNPTSGRELFKRHLPDVLVRLEKAGYETSCHATEGPGDATKAARQAAEREFDLVVAAGGDGTINEVVNGIANQ
PYRPKLGVIPVGTTNDFARAIGVPRSIEGACEVIATGEPVAIDIGSVTNEDQTRYFINIAGGGRLTELTYEVPSKLKTML
GQLAYYLKGIEMLPSIKATEAQIEYDGKLFEGEIMMFLVSLTNSVGGFEKLAPDSSLNDGLFDFIIVKKTNLAEFIRLVT
LAARGEHINDPHVIYTKANRVKVRSSMQLNLDGEFGGMLPGEFVNLYRHLEVFMPKEKAAQVRTGP
>Mature_306_residues
MKRARVIYNPTSGRELFKRHLPDVLVRLEKAGYETSCHATEGPGDATKAARQAAEREFDLVVAAGGDGTINEVVNGIANQ
PYRPKLGVIPVGTTNDFARAIGVPRSIEGACEVIATGEPVAIDIGSVTNEDQTRYFINIAGGGRLTELTYEVPSKLKTML
GQLAYYLKGIEMLPSIKATEAQIEYDGKLFEGEIMMFLVSLTNSVGGFEKLAPDSSLNDGLFDFIIVKKTNLAEFIRLVT
LAARGEHINDPHVIYTKANRVKVRSSMQLNLDGEFGGMLPGEFVNLYRHLEVFMPKEKAAQVRTGP

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=267, Percent_Identity=28.4644194756554, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 33465; Mature: 33465

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRARVIYNPTSGRELFKRHLPDVLVRLEKAGYETSCHATEGPGDATKAARQAAEREFDL
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEE
VVAAGGDGTINEVVNGIANQPYRPKLGVIPVGTTNDFARAIGVPRSIEGACEVIATGEPV
EEEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCCCCCCCEEEEECCCCE
AIDIGSVTNEDQTRYFINIAGGGRLTELTYEVPSKLKTMLGQLAYYLKGIEMLPSIKATE
EEEECCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AQIEYDGKLFEGEIMMFLVSLTNSVGGFEKLAPDSSLNDGLFDFIIVKKTNLAEFIRLVT
EEEEECCEEEHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHH
LAARGEHINDPHVIYTKANRVKVRSSMQLNLDGEFGGMLPGEFVNLYRHLEVFMPKEKAA
HHHCCCCCCCCEEEEEECCEEEEECEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHC
QVRTGP
CCCCCC
>Mature Secondary Structure
MKRARVIYNPTSGRELFKRHLPDVLVRLEKAGYETSCHATEGPGDATKAARQAAEREFDL
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCEEE
VVAAGGDGTINEVVNGIANQPYRPKLGVIPVGTTNDFARAIGVPRSIEGACEVIATGEPV
EEEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCCCCCCCEEEEECCCCE
AIDIGSVTNEDQTRYFINIAGGGRLTELTYEVPSKLKTMLGQLAYYLKGIEMLPSIKATE
EEEECCCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AQIEYDGKLFEGEIMMFLVSLTNSVGGFEKLAPDSSLNDGLFDFIIVKKTNLAEFIRLVT
EEEEECCEEEHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHH
LAARGEHINDPHVIYTKANRVKVRSSMQLNLDGEFGGMLPGEFVNLYRHLEVFMPKEKAA
HHHCCCCCCCCEEEEEECCEEEEECEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHC
QVRTGP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]