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The map label for this gene is yefA [H]

Identifier: 138893974

GI number: 138893974

Start: 338593

End: 339975

Strand: Direct

Name: yefA [H]

Synonym: GTNG_0298

Alternate gene names: 138893974

Gene position: 338593-339975 (Clockwise)

Preceding gene: 138893973

Following gene: 138893976

Centisome position: 9.54

GC content: 54.88

Gene sequence:

>1383_bases
ATGGCGAAACAACAAGCACCGGTCGCCAAAAACGACTACTATGACGTGACATTCACAGATTTGACTCACGACGGTCTTGG
CGTCGCGAAAGTGGATGGCTTTCCGTTGTTTGTGAAAAACGCGCTTCCAGGCGAGCGGGCGAAAGTGAAAGTGATCAAGG
TGAAAAAAGGGTACGGGTACGGCCGCCTCATCGAGCTGTACGAACCGAGCCCGGACCGCGTCGAGCCGCCCTGTCCCGTT
TATCGCCAATGCGGTGGCTGTCAGCTGCAACATCTAAGCTACGAAGGACAGCTGAAGGCGAAAGAAAAACAAGTGAAAGA
AGTGCTCGCCCGCATCGGCAAGCTAGAACACGTCACCGTCCATCCGGTCATCGGGATGAAGAACCCATGGCGCTACCGAA
ATAAGGCACAAGTGCCAGTCGGTGAGCGCGAAGGGGGACTGATTGCCGGATTTTACAAAGAGCGAAGTCACGAGATCATC
GACATGGACGCTTGTTTCATCCAACAGGAGAAAAATGATGTCGTCGTCCAAGCAGTGAAACGGATCGCCGAGCGCTATGG
TGTCCGACCGTACGATGAACTCACCCACCGCGGCGTGCTCCGTCATATTGTCGCCCGCTACGGCGCGGCAACAGGTGAGG
TGATGGTCGTGCTCGTCACCCGCACCGAACGTTTGCCGCACGAACAAGAGATTGTCCGCGACATCATCCGCGACATCCCT
GACGTCAAATCGATCGTGCAAAATATCAACCCTGAGCGGACGAACGTCATTTTCGGAGCGAAAACGCGGGTATTATGGGG
AAGTGAGTTCATCACCGACCGAATTGGCGACATCCAATTCGCCATCTCGGCTCGTTCATTTTACCAGGTCAACCCGGAAC
AGACGAAAGTGCTGTACGACAAAGCGCTTGAATACGCTGAGCTAACCGGAACGGAGACAGTCATTGACGCCTACTGCGGC
ATCGGCACGATCTCGCTCTTTTTAGCCAAAAAGGCTAAACATGTCTACGGCGTTGAAATCGTCCCCGAAGCGATCGAAGA
CGCCAAACGCAACGCGGCGCTCAACGGCATCACAAACGTCACGTTCGCTGTCGGCGCTGCTGAAGACGTCATCCCGCGTT
GGTACGAAGAAGGCATTCGTGCCGACTGTCTCGTCGTTGACCCTCCGCGTAAAGGCTGTGACGCGGCGCTGCTCGAGACG
ATCATGGCCATGAAACCGCCGCGCGTCGTCTATGTCTCGTGCAACCCAGCCACCTTGGCCCGCGACTTGCGCATCTTAGA
GGATGGGGGTTACGAAACGATCGAAGTCCAGCCTGTTGATATGTTTCCTCATACGGCTCATGTAGAAAGTGTAGCTCGAT
TAGCGTTAAAGATAAGAGCGTAA

Upstream 100 bases:

>100_bases
CAGCACAAGTGAGAACAGGGCCATAAGCCTGTTCTTTTCTTATGTCCTCACGCTAGAAGGGGCACGCTAAACATACACGA
TGGTCAGAAAGGGGAACGAC

Downstream 100 bases:

>100_bases
ATAAACTCTTGTTAAAAACTCTTCTTGCCAAAAACCGAAGGAAAGTTTTGCTCATATAACAAGAACATCCTTCTCTATCC
AGTTGACTGAACGGGGTAGG

Product: RNA methyltransferase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MAKQQAPVAKNDYYDVTFTDLTHDGLGVAKVDGFPLFVKNALPGERAKVKVIKVKKGYGYGRLIELYEPSPDRVEPPCPV
YRQCGGCQLQHLSYEGQLKAKEKQVKEVLARIGKLEHVTVHPVIGMKNPWRYRNKAQVPVGEREGGLIAGFYKERSHEII
DMDACFIQQEKNDVVVQAVKRIAERYGVRPYDELTHRGVLRHIVARYGAATGEVMVVLVTRTERLPHEQEIVRDIIRDIP
DVKSIVQNINPERTNVIFGAKTRVLWGSEFITDRIGDIQFAISARSFYQVNPEQTKVLYDKALEYAELTGTETVIDAYCG
IGTISLFLAKKAKHVYGVEIVPEAIEDAKRNAALNGITNVTFAVGAAEDVIPRWYEEGIRADCLVVDPPRKGCDAALLET
IMAMKPPRVVYVSCNPATLARDLRILEDGGYETIEVQPVDMFPHTAHVESVARLALKIRA

Sequences:

>Translated_460_residues
MAKQQAPVAKNDYYDVTFTDLTHDGLGVAKVDGFPLFVKNALPGERAKVKVIKVKKGYGYGRLIELYEPSPDRVEPPCPV
YRQCGGCQLQHLSYEGQLKAKEKQVKEVLARIGKLEHVTVHPVIGMKNPWRYRNKAQVPVGEREGGLIAGFYKERSHEII
DMDACFIQQEKNDVVVQAVKRIAERYGVRPYDELTHRGVLRHIVARYGAATGEVMVVLVTRTERLPHEQEIVRDIIRDIP
DVKSIVQNINPERTNVIFGAKTRVLWGSEFITDRIGDIQFAISARSFYQVNPEQTKVLYDKALEYAELTGTETVIDAYCG
IGTISLFLAKKAKHVYGVEIVPEAIEDAKRNAALNGITNVTFAVGAAEDVIPRWYEEGIRADCLVVDPPRKGCDAALLET
IMAMKPPRVVYVSCNPATLARDLRILEDGGYETIEVQPVDMFPHTAHVESVARLALKIRA
>Mature_459_residues
AKQQAPVAKNDYYDVTFTDLTHDGLGVAKVDGFPLFVKNALPGERAKVKVIKVKKGYGYGRLIELYEPSPDRVEPPCPVY
RQCGGCQLQHLSYEGQLKAKEKQVKEVLARIGKLEHVTVHPVIGMKNPWRYRNKAQVPVGEREGGLIAGFYKERSHEIID
MDACFIQQEKNDVVVQAVKRIAERYGVRPYDELTHRGVLRHIVARYGAATGEVMVVLVTRTERLPHEQEIVRDIIRDIPD
VKSIVQNINPERTNVIFGAKTRVLWGSEFITDRIGDIQFAISARSFYQVNPEQTKVLYDKALEYAELTGTETVIDAYCGI
GTISLFLAKKAKHVYGVEIVPEAIEDAKRNAALNGITNVTFAVGAAEDVIPRWYEEGIRADCLVVDPPRKGCDAALLETI
MAMKPPRVVYVSCNPATLARDLRILEDGGYETIEVQPVDMFPHTAHVESVARLALKIRA

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=196, Percent_Identity=38.265306122449, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI51173878, Length=196, Percent_Identity=38.265306122449, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI269784680, Length=414, Percent_Identity=26.0869565217391, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI269784676, Length=414, Percent_Identity=26.0869565217391, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI20149688, Length=414, Percent_Identity=26.0869565217391, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI269784678, Length=353, Percent_Identity=25.2124645892351, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1789148, Length=451, Percent_Identity=32.5942350332594, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1787083, Length=381, Percent_Identity=29.9212598425197, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1790403, Length=379, Percent_Identity=25.8575197889182, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71997438, Length=163, Percent_Identity=31.9018404907975, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322909, Length=164, Percent_Identity=32.9268292682927, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24666579, Length=419, Percent_Identity=31.2649164677804, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 51507; Mature: 51376

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQQAPVAKNDYYDVTFTDLTHDGLGVAKVDGFPLFVKNALPGERAKVKVIKVKKGYGY
CCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCEEEEECCCCCCCCEEEEEEEECCCCC
GRLIELYEPSPDRVEPPCPVYRQCGGCQLQHLSYEGQLKAKEKQVKEVLARIGKLEHVTV
CEEEEEECCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEE
HPVIGMKNPWRYRNKAQVPVGEREGGLIAGFYKERSHEIIDMDACFIQQEKNDVVVQAVK
EEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEECHHHEEECCCCHHHHHHHH
RIAERYGVRPYDELTHRGVLRHIVARYGAATGEVMVVLVTRTERLPHEQEIVRDIIRDIP
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCH
DVKSIVQNINPERTNVIFGAKTRVLWGSEFITDRIGDIQFAISARSFYQVNPEQTKVLYD
HHHHHHHHCCCCCCEEEEECCEEEEECCHHHHHCCCCEEEEEEECEEEEECHHHHHHHHH
KALEYAELTGTETVIDAYCGIGTISLFLAKKAKHVYGVEIVPEAIEDAKRNAALNGITNV
HHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCEECEEECHHHHHHHHHHHHHCCCCEE
TFAVGAAEDVIPRWYEEGIRADCLVVDPPRKGCDAALLETIMAMKPPRVVYVSCNPATLA
EEEECCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCHHHHH
RDLRILEDGGYETIEVQPVDMFPHTAHVESVARLALKIRA
HHHEEEECCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKQQAPVAKNDYYDVTFTDLTHDGLGVAKVDGFPLFVKNALPGERAKVKVIKVKKGYGY
CCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCEEEEECCCCCCCCEEEEEEEECCCCC
GRLIELYEPSPDRVEPPCPVYRQCGGCQLQHLSYEGQLKAKEKQVKEVLARIGKLEHVTV
CEEEEEECCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEE
HPVIGMKNPWRYRNKAQVPVGEREGGLIAGFYKERSHEIIDMDACFIQQEKNDVVVQAVK
EEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEECHHHEEECCCCHHHHHHHH
RIAERYGVRPYDELTHRGVLRHIVARYGAATGEVMVVLVTRTERLPHEQEIVRDIIRDIP
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCH
DVKSIVQNINPERTNVIFGAKTRVLWGSEFITDRIGDIQFAISARSFYQVNPEQTKVLYD
HHHHHHHHCCCCCCEEEEECCEEEEECCHHHHHCCCCEEEEEEECEEEEECHHHHHHHHH
KALEYAELTGTETVIDAYCGIGTISLFLAKKAKHVYGVEIVPEAIEDAKRNAALNGITNV
HHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCEECEEECHHHHHHHHHHHHHCCCCEE
TFAVGAAEDVIPRWYEEGIRADCLVVDPPRKGCDAALLETIMAMKPPRVVYVSCNPATLA
EEEECCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCHHHHH
RDLRILEDGGYETIEVQPVDMFPHTAHVESVARLALKIRA
HHHEEEECCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]