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The map label for this gene is aprX [H]

Identifier: 138893864

GI number: 138893864

Start: 199249

End: 200577

Strand: Direct

Name: aprX [H]

Synonym: GTNG_0188

Alternate gene names: 138893864

Gene position: 199249-200577 (Clockwise)

Preceding gene: 138893863

Following gene: 138893866

Centisome position: 5.61

GC content: 53.2

Gene sequence:

>1329_bases
GTGTTTGGCTATTCGATTGTGCAGTTAGCCCGCCGTCATGCGGGGCGGCTTGATCACCCTCTCCGCCATACGCTTGTCAA
CATGTACAAACCGATGACACGCATGCCATGCATTTTCCACCGTTGGATCGAGCGCTGGCTGCGGAAGTGGCGGACTGTTT
CTGTTCTGATCGAGGTTGATGACGATGAAGGGATTGAAGCGCTCACCGAGGCGGAACGGAATCATTTTCGCATGAAAACG
CATCACCATTTCCGTCATGTCCCGATATGGAGCGCGACAGTGACACCGGCAGCACTTGAGCAGTTGCTGGAGAATCCAAA
AGTGAAAAAAGTGTATCTCAACCGCACGATCAAGGCGGTATTAAACAACGCGGTGCCATCGGCCAATGCGAAACACGTCA
CTGTCAATGAAACAGAGCTGAGCGGCAAAGGAGTGACGATTGCGATTGTCGACACAGGCATTGACCCTCATCCGGATTTA
GAGGGGCGGATTACGGCGTTTGTCGACTTGGTGAATGGAAAAACGGCACCGTATGATGATAACGGACATGGAACTCACTG
CGCTGGCGATGCGGCCGGAAACGGACGCATGTCGGACGGGCTATACGCCGGGCCAGCGTATGAGGCGAACCTCGTTGGCG
TCAAAGTGCTCAATCGCTCAGGGATGGGTACGCTAGAAACCATTATGCGCGGTATTGAATGGTGCATCGACTACAACAAG
AAAAACCCGGCCAATCCAATTCATATTATTTCTTTATCGCTTGGTGGGGAACCACAGCCGTTCCCGGCTGAAAATGACGA
TCCACTTGTACAAGTCGCTGAGCGGGCATGGGAAAATGGCATTGTTGTCTGTGCGGCAGCGGGAAATGAGGGGCCGAATT
ATGGGACGATTGCCAGCCCAGGCATTAGCGACCGCATCATTACTGTCGGTGCCCTTGATGACCGTGATACAGCGTCAACA
CGCACCGATGATGCTGTAGCGCCTTTTTCGAGCCGGGGGCCGACAAGATATGGTGTGACAAAACCGGATCTGGTCGTTCC
CGGGGTCAACATTATATCGCTTCGTGCCCCACGTTCGTTTCTTGACAAGATGAATAAGCAAAGCCGGGTTGGTGACTATT
ATATGTCGATGTCCGGCACATCGATGGCGACGCCGATTTGTGCTGGCATTGTTGCTTTAATGCTTCAGGCCAAACCAGAT
GCAACGCCGGACGAGATCAAGCAGGCGTTAAGGGACAGTGCCGATTTATGGAAAGACCGCGACCCGAACGTATATGGGGC
GGGTTACGTGAATGCGAAGCGGGCCATCGAACAGTTACTGAAGCGTTAG

Upstream 100 bases:

>100_bases
GAGGCAAGCGTTTTTTCGTCGGTTCTAGTGGAAATGCCGCTTCCTTCTTTGTCCATTCATCATATGAGTATAGCAATCAA
CGAAGGAAGGGGGAAGGGCA

Downstream 100 bases:

>100_bases
AAAAAAGAGGGAGGCGCTTATTGAGCGCTTCCCTCTTTGCTTTCGCGGTTGTTGTAGCCTCCGCGGTTGTTATAGTCGTA
TTTCGACGGGTCGACCGGCT

Product: intracellular alkaline serine protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MFGYSIVQLARRHAGRLDHPLRHTLVNMYKPMTRMPCIFHRWIERWLRKWRTVSVLIEVDDDEGIEALTEAERNHFRMKT
HHHFRHVPIWSATVTPAALEQLLENPKVKKVYLNRTIKAVLNNAVPSANAKHVTVNETELSGKGVTIAIVDTGIDPHPDL
EGRITAFVDLVNGKTAPYDDNGHGTHCAGDAAGNGRMSDGLYAGPAYEANLVGVKVLNRSGMGTLETIMRGIEWCIDYNK
KNPANPIHIISLSLGGEPQPFPAENDDPLVQVAERAWENGIVVCAAAGNEGPNYGTIASPGISDRIITVGALDDRDTAST
RTDDAVAPFSSRGPTRYGVTKPDLVVPGVNIISLRAPRSFLDKMNKQSRVGDYYMSMSGTSMATPICAGIVALMLQAKPD
ATPDEIKQALRDSADLWKDRDPNVYGAGYVNAKRAIEQLLKR

Sequences:

>Translated_442_residues
MFGYSIVQLARRHAGRLDHPLRHTLVNMYKPMTRMPCIFHRWIERWLRKWRTVSVLIEVDDDEGIEALTEAERNHFRMKT
HHHFRHVPIWSATVTPAALEQLLENPKVKKVYLNRTIKAVLNNAVPSANAKHVTVNETELSGKGVTIAIVDTGIDPHPDL
EGRITAFVDLVNGKTAPYDDNGHGTHCAGDAAGNGRMSDGLYAGPAYEANLVGVKVLNRSGMGTLETIMRGIEWCIDYNK
KNPANPIHIISLSLGGEPQPFPAENDDPLVQVAERAWENGIVVCAAAGNEGPNYGTIASPGISDRIITVGALDDRDTAST
RTDDAVAPFSSRGPTRYGVTKPDLVVPGVNIISLRAPRSFLDKMNKQSRVGDYYMSMSGTSMATPICAGIVALMLQAKPD
ATPDEIKQALRDSADLWKDRDPNVYGAGYVNAKRAIEQLLKR
>Mature_442_residues
MFGYSIVQLARRHAGRLDHPLRHTLVNMYKPMTRMPCIFHRWIERWLRKWRTVSVLIEVDDDEGIEALTEAERNHFRMKT
HHHFRHVPIWSATVTPAALEQLLENPKVKKVYLNRTIKAVLNNAVPSANAKHVTVNETELSGKGVTIAIVDTGIDPHPDL
EGRITAFVDLVNGKTAPYDDNGHGTHCAGDAAGNGRMSDGLYAGPAYEANLVGVKVLNRSGMGTLETIMRGIEWCIDYNK
KNPANPIHIISLSLGGEPQPFPAENDDPLVQVAERAWENGIVVCAAAGNEGPNYGTIASPGISDRIITVGALDDRDTAST
RTDDAVAPFSSRGPTRYGVTKPDLVVPGVNIISLRAPRSFLDKMNKQSRVGDYYMSMSGTSMATPICAGIVALMLQAKPD
ATPDEIKQALRDSADLWKDRDPNVYGAGYVNAKRAIEQLLKR

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=287, Percent_Identity=29.616724738676, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI31317307, Length=281, Percent_Identity=28.1138790035587, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17533391, Length=273, Percent_Identity=27.4725274725275, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319893, Length=276, Percent_Identity=33.695652173913, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6320775, Length=295, Percent_Identity=32.2033898305085, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324576, Length=285, Percent_Identity=32.9824561403509, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45550681, Length=385, Percent_Identity=27.5324675324675, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 48558; Mature: 48558

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGYSIVQLARRHAGRLDHPLRHTLVNMYKPMTRMPCIFHRWIERWLRKWRTVSVLIEVD
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHCCEEEEEEEEC
DDEGIEALTEAERNHFRMKTHHHFRHVPIWSATVTPAALEQLLENPKVKKVYLNRTIKAV
CCCCHHHHHHHHHHHEEEHHCCCEEECCEECCCCCHHHHHHHHCCCCEEEHHHHHHHHHH
LNNAVPSANAKHVTVNETELSGKGVTIAIVDTGIDPHPDLEGRITAFVDLVNGKTAPYDD
HHHCCCCCCCCEEEEECCEECCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCC
NGHGTHCAGDAAGNGRMSDGLYAGPAYEANLVGVKVLNRSGMGTLETIMRGIEWCIDYNK
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCC
KNPANPIHIISLSLGGEPQPFPAENDDPLVQVAERAWENGIVVCAAAGNEGPNYGTIASP
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
GISDRIITVGALDDRDTASTRTDDAVAPFSSRGPTRYGVTKPDLVVPGVNIISLRAPRSF
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEECCCHHH
LDKMNKQSRVGDYYMSMSGTSMATPICAGIVALMLQAKPDATPDEIKQALRDSADLWKDR
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
DPNVYGAGYVNAKRAIEQLLKR
CCCEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MFGYSIVQLARRHAGRLDHPLRHTLVNMYKPMTRMPCIFHRWIERWLRKWRTVSVLIEVD
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHCCEEEEEEEEC
DDEGIEALTEAERNHFRMKTHHHFRHVPIWSATVTPAALEQLLENPKVKKVYLNRTIKAV
CCCCHHHHHHHHHHHEEEHHCCCEEECCEECCCCCHHHHHHHHCCCCEEEHHHHHHHHHH
LNNAVPSANAKHVTVNETELSGKGVTIAIVDTGIDPHPDLEGRITAFVDLVNGKTAPYDD
HHHCCCCCCCCEEEEECCEECCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCC
NGHGTHCAGDAAGNGRMSDGLYAGPAYEANLVGVKVLNRSGMGTLETIMRGIEWCIDYNK
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCC
KNPANPIHIISLSLGGEPQPFPAENDDPLVQVAERAWENGIVVCAAAGNEGPNYGTIASP
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
GISDRIITVGALDDRDTASTRTDDAVAPFSSRGPTRYGVTKPDLVVPGVNIISLRAPRSF
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEECCCHHH
LDKMNKQSRVGDYYMSMSGTSMATPICAGIVALMLQAKPDATPDEIKQALRDSADLWKDR
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
DPNVYGAGYVNAKRAIEQLLKR
CCCEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]