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The map label for this gene is cwlM [H]

Identifier: 138893863

GI number: 138893863

Start: 198393

End: 199124

Strand: Direct

Name: cwlM [H]

Synonym: GTNG_0187

Alternate gene names: 138893863

Gene position: 198393-199124 (Clockwise)

Preceding gene: 138893862

Following gene: 138893864

Centisome position: 5.59

GC content: 50.96

Gene sequence:

>732_bases
ATGCCGAAGATTTTTCTTGATCCAGGGCATGGGGGCAATGATACGGGCGCGGTCGGCAACGGATTGCGTGAGAAGGATAT
CACGCTGTCCATCGCCTTGGAGATAAGCCGCATTTTGCAAAACGAGTACGAAGGAGTCTCGGTGCAGCTGAGCCGAACGA
AAGATGAAACGGTATCGCTTGCTGAACGGACGAGGCGGGCCAATAGTTGGGGAGCCGATCTATATGTGTCAATTCACGTG
AATGCGGGCGGTGGCACAGGATTTGAAGACTACATTTACCATGGGCTGTCGGAAAGTTCCCAAACGGCCCGCATTCGCGA
TGTGCTGCATGAAGAAATTGTCCGAGCGACTCAATTCCGCGACCGCGGCAAAAAGAAAGCAAATTTTCATGTGCTAAGGG
AAACGAAAATGTCGGCGGTGTTGACGGAAAACGGGTTTATCGACCATAAGGAAGACGCAAAACAATTGACCGACCCTAAT
TTTTTGCGGATGATTGCCCGCGGTCATGTCAACGGGTTGGAACGGGCACTTGGATTGAAGAAGAAAGCAAGTAACCCGAA
CAATCCTCCTAGAGATCCAGACAAAGGCATGCCGAGCACTGGCCGTTTGACGCGTGTCATCGTGGATGGGAAGCAAGTCG
GTGCTTTTGTTGAACCAGACAACGTGTTGCGCCAAGTGGAGCATTATTTAGGAAGAGCGAAACGGATTGTGCTTGAGCAA
GTAGAGGAATGA

Upstream 100 bases:

>100_bases
CCTGGGGAAGTATCTATGGCATTTTGGAATGACATGCTTATTCTAATAGTAACAAATTCTAATAGTAACAAACTCCCAAA
TGACGGAAAGGGGAAGGGAG

Downstream 100 bases:

>100_bases
TGGGAACGCTTGCCTAGTTTTAATGAGGCAAGCGTTTTTTCGTCGGTTCTAGTGGAAATGCCGCTTCCTTCTTTGTCCAT
TCATCATATGAGTATAGCAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MPKIFLDPGHGGNDTGAVGNGLREKDITLSIALEISRILQNEYEGVSVQLSRTKDETVSLAERTRRANSWGADLYVSIHV
NAGGGTGFEDYIYHGLSESSQTARIRDVLHEEIVRATQFRDRGKKKANFHVLRETKMSAVLTENGFIDHKEDAKQLTDPN
FLRMIARGHVNGLERALGLKKKASNPNNPPRDPDKGMPSTGRLTRVIVDGKQVGAFVEPDNVLRQVEHYLGRAKRIVLEQ
VEE

Sequences:

>Translated_243_residues
MPKIFLDPGHGGNDTGAVGNGLREKDITLSIALEISRILQNEYEGVSVQLSRTKDETVSLAERTRRANSWGADLYVSIHV
NAGGGTGFEDYIYHGLSESSQTARIRDVLHEEIVRATQFRDRGKKKANFHVLRETKMSAVLTENGFIDHKEDAKQLTDPN
FLRMIARGHVNGLERALGLKKKASNPNNPPRDPDKGMPSTGRLTRVIVDGKQVGAFVEPDNVLRQVEHYLGRAKRIVLEQ
VEE
>Mature_242_residues
PKIFLDPGHGGNDTGAVGNGLREKDITLSIALEISRILQNEYEGVSVQLSRTKDETVSLAERTRRANSWGADLYVSIHVN
AGGGTGFEDYIYHGLSESSQTARIRDVLHEEIVRATQFRDRGKKKANFHVLRETKMSAVLTENGFIDHKEDAKQLTDPNF
LRMIARGHVNGLERALGLKKKASNPNNPPRDPDKGMPSTGRLTRVIVDGKQVGAFVEPDNVLRQVEHYLGRAKRIVLEQV
EE

Specific function: Hydrolyzes the cell wall of M.luteus more efficiently than that of B.licheniformis and B.subtilis. The C-terminal region, including the repeats, determines substrate specificity [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Probable). Note=Accumulates in cells as inclusion bodies. May be secreted by a mechanism different from the normal export system [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=216, Percent_Identity=28.2407407407407, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87082163, Length=216, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 27064; Mature: 26933

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKIFLDPGHGGNDTGAVGNGLREKDITLSIALEISRILQNEYEGVSVQLSRTKDETVSL
CCCEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
AERTRRANSWGADLYVSIHVNAGGGTGFEDYIYHGLSESSQTARIRDVLHEEIVRATQFR
HHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
DRGKKKANFHVLRETKMSAVLTENGFIDHKEDAKQLTDPNFLRMIARGHVNGLERALGLK
HCCCCCCCCEEEHHHHHHHHEECCCCCCCCHHHHHCCCHHHHHHHHHHCHHHHHHHHCCH
KKASNPNNPPRDPDKGMPSTGRLTRVIVDGKQVGAFVEPDNVLRQVEHYLGRAKRIVLEQ
HHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCEECHHHHHHHHHHHHHHHHHHHHHH
VEE
HCC
>Mature Secondary Structure 
PKIFLDPGHGGNDTGAVGNGLREKDITLSIALEISRILQNEYEGVSVQLSRTKDETVSL
CCEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
AERTRRANSWGADLYVSIHVNAGGGTGFEDYIYHGLSESSQTARIRDVLHEEIVRATQFR
HHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
DRGKKKANFHVLRETKMSAVLTENGFIDHKEDAKQLTDPNFLRMIARGHVNGLERALGLK
HCCCCCCCCEEEHHHHHHHHEECCCCCCCCHHHHHCCCHHHHHHHHHHCHHHHHHHHCCH
KKASNPNNPPRDPDKGMPSTGRLTRVIVDGKQVGAFVEPDNVLRQVEHYLGRAKRIVLEQ
HHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCEECHHHHHHHHHHHHHHHHHHHHHH
VEE
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1495475 [H]