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The map label for this gene is ddl

Identifier: 126696830

GI number: 126696830

Start: 1262469

End: 1263536

Strand: Direct

Name: ddl

Synonym: P9301_14921

Alternate gene names: 126696830

Gene position: 1262469-1263536 (Clockwise)

Preceding gene: 126696829

Following gene: 126696831

Centisome position: 76.89

GC content: 29.4

Gene sequence:

>1068_bases
ATGATCGGGGAAAAGAAAAAATGTATTGGGTTAATATTTGGCGGGCATTCCAATGAACATGAAGTATCGATATCCTCTGC
AAAAACAGTTTTTAAAGCATTTAAAGCACAAATAAATAGAGAACGCTTTACAGTTAAAGCCTTTTACATAAACAAATATG
GAGATTGGCTTGATAGTGATATTTCAGAAAAAATCCTAACTGGTGAAATTGAAAACTATAAAACAAAAAAACAAGAAATT
TTTAATCAAGAAAAAATTAACTTCCTTGACGGAATAGAATTTCAAAATATTGATGTTTGGTTTCCTCTTTTACATGGATT
TAATGGTGAAGACGGATCAATTCATGGCTTAATTAGATTTACAAAGAAACCTTTAGTCGGGTGCGGAATTATTGGCTCTG
CACTTGGAATGGATAAAATATTGATGAAAACAATTTTCTCAAATCATAAACTGCCACAAGTTAATTACCTAGTTTTTCAA
AATGAAGATCTCAACGATAAACAAGTAAAAAATAAAATTATTAATGAAATTTTAAAAAAATTAAAATTTCCTGTTTTTGT
TAAACCATCGAACTCTGGATCATCTCTTGGCATCTCCAAAGTCAAAAATGAATCGGAAATATTACTAGCATTAGAAAAGG
CTTGGGGAATTGATCCAAGAATCTTAATAGAGGAAGGTTTAGAGGTAAGGGAAATTGAATGCGGAATAATTGGGAATTCA
AAACTCATAACCTCTGAAATAGGCGAGGTAAATTACGAAAGTGATTGGTATGATTACGATTCAAAATATCACTCAAATAA
TAAAATAATTATCCCAGCCGAAATAGATTCTAAAATCACAAACGAAATTAAAGAAATTGCTATTAAAAGTTGTAGAGCAC
TAAATATTTTCGGTTTTGCAAGAGTAGATTTCTTTTTAGAAAAATCTTCAAATAAAATTTTACTAAATGAAATAAATACA
ATTCCTGGTTTTACAAAAAACAGTATGTTTCCAATGCTTTGGGAAGCTTCAGGTTTAAAAATTGAACAACTTGTGGCTAA
ACTGGTAGATATATCTTTAGATTTGTAA

Upstream 100 bases:

>100_bases
AACTTTAATTTTTCTTTGGGAGATGAGATAAATGTCAGAATAAATGAAGCAAGACCTTTTTCTTTAACAGGAGAACTTTC
TTTATAAAAATTTTTTTTAA

Downstream 100 bases:

>100_bases
TTTAAATAAAATGTTGCATGGACTACTTTGGTTACCATTACTTTTAATCTTCATTTTATTAACTGCACTTGGATGGTTAG
AAAGAAGAAGGCAAAATCTT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MIGEKKKCIGLIFGGHSNEHEVSISSAKTVFKAFKAQINRERFTVKAFYINKYGDWLDSDISEKILTGEIENYKTKKQEI
FNQEKINFLDGIEFQNIDVWFPLLHGFNGEDGSIHGLIRFTKKPLVGCGIIGSALGMDKILMKTIFSNHKLPQVNYLVFQ
NEDLNDKQVKNKIINEILKKLKFPVFVKPSNSGSSLGISKVKNESEILLALEKAWGIDPRILIEEGLEVREIECGIIGNS
KLITSEIGEVNYESDWYDYDSKYHSNNKIIIPAEIDSKITNEIKEIAIKSCRALNIFGFARVDFFLEKSSNKILLNEINT
IPGFTKNSMFPMLWEASGLKIEQLVAKLVDISLDL

Sequences:

>Translated_355_residues
MIGEKKKCIGLIFGGHSNEHEVSISSAKTVFKAFKAQINRERFTVKAFYINKYGDWLDSDISEKILTGEIENYKTKKQEI
FNQEKINFLDGIEFQNIDVWFPLLHGFNGEDGSIHGLIRFTKKPLVGCGIIGSALGMDKILMKTIFSNHKLPQVNYLVFQ
NEDLNDKQVKNKIINEILKKLKFPVFVKPSNSGSSLGISKVKNESEILLALEKAWGIDPRILIEEGLEVREIECGIIGNS
KLITSEIGEVNYESDWYDYDSKYHSNNKIIIPAEIDSKITNEIKEIAIKSCRALNIFGFARVDFFLEKSSNKILLNEINT
IPGFTKNSMFPMLWEASGLKIEQLVAKLVDISLDL
>Mature_355_residues
MIGEKKKCIGLIFGGHSNEHEVSISSAKTVFKAFKAQINRERFTVKAFYINKYGDWLDSDISEKILTGEIENYKTKKQEI
FNQEKINFLDGIEFQNIDVWFPLLHGFNGEDGSIHGLIRFTKKPLVGCGIIGSALGMDKILMKTIFSNHKLPQVNYLVFQ
NEDLNDKQVKNKIINEILKKLKFPVFVKPSNSGSSLGISKVKNESEILLALEKAWGIDPRILIEEGLEVREIECGIIGNS
KLITSEIGEVNYESDWYDYDSKYHSNNKIIIPAEIDSKITNEIKEIAIKSCRALNIFGFARVDFFLEKSSNKILLNEINT
IPGFTKNSMFPMLWEASGLKIEQLVAKLVDISLDL

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=357, Percent_Identity=36.4145658263305, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1786280, Length=345, Percent_Identity=28.695652173913, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_PROM0 (A3PEE0)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091716.1
- ProteinModelPortal:   A3PEE0
- SMR:   A3PEE0
- STRING:   A3PEE0
- GeneID:   4912719
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_14921
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   PMAR167546:P9301ORF_1517-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 40279; Mature: 40279

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGEKKKCIGLIFGGHSNEHEVSISSAKTVFKAFKAQINRERFTVKAFYINKYGDWLDSD
CCCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHCCH
ISEKILTGEIENYKTKKQEIFNQEKINFLDGIEFQNIDVWFPLLHGFNGEDGSIHGLIRF
HHHHHHHCCHHHHHHHHHHHHCHHHCHHHCCCCCCCCEEEEHHHCCCCCCCCCEEEEEEE
TKKPLVGCGIIGSALGMDKILMKTIFSNHKLPQVNYLVFQNEDLNDKQVKNKIINEILKK
CCCCCEECCHHHHHHCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
LKFPVFVKPSNSGSSLGISKVKNESEILLALEKAWGIDPRILIEEGLEVREIECGIIGNS
CCCCEEEEECCCCCCCCHHHCCCCCCEEEEEHHHCCCCCEEEECCCCCEEEEEEEEECCC
KLITSEIGEVNYESDWYDYDSKYHSNNKIIIPAEIDSKITNEIKEIAIKSCRALNIFGFA
EEEEHHHCCCCCCCCCCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEE
RVDFFLEKSSNKILLNEINTIPGFTKNSMFPMLWEASGLKIEQLVAKLVDISLDL
EEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIGEKKKCIGLIFGGHSNEHEVSISSAKTVFKAFKAQINRERFTVKAFYINKYGDWLDSD
CCCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHCCH
ISEKILTGEIENYKTKKQEIFNQEKINFLDGIEFQNIDVWFPLLHGFNGEDGSIHGLIRF
HHHHHHHCCHHHHHHHHHHHHCHHHCHHHCCCCCCCCEEEEHHHCCCCCCCCCEEEEEEE
TKKPLVGCGIIGSALGMDKILMKTIFSNHKLPQVNYLVFQNEDLNDKQVKNKIINEILKK
CCCCCEECCHHHHHHCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
LKFPVFVKPSNSGSSLGISKVKNESEILLALEKAWGIDPRILIEEGLEVREIECGIIGNS
CCCCEEEEECCCCCCCCHHHCCCCCCEEEEEHHHCCCCCEEEECCCCCEEEEEEEEECCC
KLITSEIGEVNYESDWYDYDSKYHSNNKIIIPAEIDSKITNEIKEIAIKSCRALNIFGFA
EEEEHHHCCCCCCCCCCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEE
RVDFFLEKSSNKILLNEINTIPGFTKNSMFPMLWEASGLKIEQLVAKLVDISLDL
EEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA