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The map label for this gene is yjfH [C]

Identifier: 126696822

GI number: 126696822

Start: 1252718

End: 1253431

Strand: Reverse

Name: yjfH [C]

Synonym: P9301_14841

Alternate gene names: 126696822

Gene position: 1253431-1252718 (Counterclockwise)

Preceding gene: 126696824

Following gene: 126696821

Centisome position: 76.34

GC content: 32.77

Gene sequence:

>714_bases
TTGCTTAAGTCTGGAAATTCTCCCTCCAAAATTTTAGTTACTGAAAAATGGCTAATAAAAAACCAAAATCTTAGCAAACA
GTTTGATGAATCATTATTAACTTTGGTCTCTGAGGAGGTTTTAGCTTCTGCGATTTCAACAGTTAATCCAGATGGCATCG
CAGCATTAGTAGAAATTTCATCGATACCTAATTATCAATTCAATAGTAAAGATGATTTTATTCTTGTTCTTGATAGAATT
CAAGATCCTGGGAATATGGGTAATCTTTTTAGAACTGCTTTAGCTGCTGGTGTCGATGCAATTTTTTTAGCTGGAGGTGC
GCACCCATTAAACCAAAAAGTATTAAGAGCATCAACTGGCGCGGTCTTTAATCTCCCATTTAAAAGATATGAGGGTACTG
AAGAAGAAATAATAAATTCTTTATTAAAGTCTTTATCTGAATTATCAAATGAAGGATTTAAGATTTTTTCCACTAGTAGC
TTTAATAAAAGCTCAAAAAAAACTTCAAAACCATACTGGGAAATTGATTGGACTAAGTCTACTGTCTTGATTTTGGGTAA
TGAAGGTCAAGGTATTCATAAGAAAATTCAAGAAGCTTTTAATGAAACAATTACAATTCCGCATAGTGAGCTTGTAGAAT
CATTGAATGTGGCTTGTGTTGCAGTTCCATTATTACTAGAACGAAAAAGAGTCGCATACACCTCTAATAAATAA

Upstream 100 bases:

>100_bases
AAACAATAATCTAGTTAAAAGATTTAGATCATTTAAAAGCGGATCCTCTCGCAAAGATAAAGATTTTTTTTGTATAGAAG
GTACCCATCTTATTGAGGAA

Downstream 100 bases:

>100_bases
ATAAAAAGTGACTGATTCAAGTTTTGATTTCGATTTAATTGTAATAGGAGCAGGATATGGAGGTTTTGATGCCGCTAAAC
ATGCTGCTGGCAAGGGACTG

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MLKSGNSPSKILVTEKWLIKNQNLSKQFDESLLTLVSEEVLASAISTVNPDGIAALVEISSIPNYQFNSKDDFILVLDRI
QDPGNMGNLFRTALAAGVDAIFLAGGAHPLNQKVLRASTGAVFNLPFKRYEGTEEEIINSLLKSLSELSNEGFKIFSTSS
FNKSSKKTSKPYWEIDWTKSTVLILGNEGQGIHKKIQEAFNETITIPHSELVESLNVACVAVPLLLERKRVAYTSNK

Sequences:

>Translated_237_residues
MLKSGNSPSKILVTEKWLIKNQNLSKQFDESLLTLVSEEVLASAISTVNPDGIAALVEISSIPNYQFNSKDDFILVLDRI
QDPGNMGNLFRTALAAGVDAIFLAGGAHPLNQKVLRASTGAVFNLPFKRYEGTEEEIINSLLKSLSELSNEGFKIFSTSS
FNKSSKKTSKPYWEIDWTKSTVLILGNEGQGIHKKIQEAFNETITIPHSELVESLNVACVAVPLLLERKRVAYTSNK
>Mature_237_residues
MLKSGNSPSKILVTEKWLIKNQNLSKQFDESLLTLVSEEVLASAISTVNPDGIAALVEISSIPNYQFNSKDDFILVLDRI
QDPGNMGNLFRTALAAGVDAIFLAGGAHPLNQKVLRASTGAVFNLPFKRYEGTEEEIINSLLKSLSELSNEGFKIFSTSS
FNKSSKKTSKPYWEIDWTKSTVLILGNEGQGIHKKIQEAFNETITIPHSELVESLNVACVAVPLLLERKRVAYTSNK

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=170, Percent_Identity=27.0588235294118, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26103; Mature: 26103

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSGNSPSKILVTEKWLIKNQNLSKQFDESLLTLVSEEVLASAISTVNPDGIAALVEIS
CCCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
SIPNYQFNSKDDFILVLDRIQDPGNMGNLFRTALAAGVDAIFLAGGAHPLNQKVLRASTG
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCC
AVFNLPFKRYEGTEEEIINSLLKSLSELSNEGFKIFSTSSFNKSSKKTSKPYWEIDWTKS
CEEECCHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEECCE
TVLILGNEGQGIHKKIQEAFNETITIPHSELVESLNVACVAVPLLLERKRVAYTSNK
EEEEECCCCCHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKSGNSPSKILVTEKWLIKNQNLSKQFDESLLTLVSEEVLASAISTVNPDGIAALVEIS
CCCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
SIPNYQFNSKDDFILVLDRIQDPGNMGNLFRTALAAGVDAIFLAGGAHPLNQKVLRASTG
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCC
AVFNLPFKRYEGTEEEIINSLLKSLSELSNEGFKIFSTSSFNKSSKKTSKPYWEIDWTKS
CEEECCHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEECCE
TVLILGNEGQGIHKKIQEAFNETITIPHSELVESLNVACVAVPLLLERKRVAYTSNK
EEEEECCCCCHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]